Ischemia–reperfusion injury is a common pathophysiological mechanism in retinal degeneration.PANoptosis is a newly defined integral form of regulated cell death that combines the key features of pyroptosis,apoptosis,...Ischemia–reperfusion injury is a common pathophysiological mechanism in retinal degeneration.PANoptosis is a newly defined integral form of regulated cell death that combines the key features of pyroptosis,apoptosis,and necroptosis.Oligomerization of mitochondrial voltage-dependent anion channel 1 is an important pathological event in regulating cell death in retinal ischemia–reperfusion injury.However,its role in PANoptosis remains largely unknown.In this study,we demonstrated that voltage-dependent anion channel 1 oligomerization-mediated mitochondrial dysfunction was associated with PANoptosis in retinal ischemia–reperfusion injury.Inhibition of voltage-dependent anion channel 1 oligomerization suppressed mitochondrial dysfunction and PANoptosis in retinal cells subjected to ischemia–reperfusion injury.Mechanistically,mitochondria-derived reactive oxygen species played a central role in the voltagedependent anion channel 1-mediated regulation of PANoptosis by promoting PANoptosome assembly.Moreover,inhibiting voltage-dependent anion channel 1 oligomerization protected against PANoptosis in the retinas of rats subjected to ischemia–reperfusion injury.Overall,our findings reveal the critical role of voltage-dependent anion channel 1 oligomerization in regulating PANoptosis in retinal ischemia–reperfusion injury,highlighting voltage-dependent anion channel 1 as a promising therapeutic target.展开更多
Heavy metal concentrations in urban soils are likely to increase over time because of contin-uous urbanization and heavy metal emissions.To estimate the accumulation rates of heavy metals in urban soils,we collected s...Heavy metal concentrations in urban soils are likely to increase over time because of contin-uous urbanization and heavy metal emissions.To estimate the accumulation rates of heavy metals in urban soils,we collected soil samples from residential areas with different build-ing ages in the metropolitan cities of Shanghai,Shenzhen,and Beijing,China.Heavy metal concentrations in the soils varied among the cities and were primarily affected by soil parent material and the intensity of anthropogenic sources.Regression analyses revealed that the accumulation rates of Cd and Cu in the soils ranged from 0.0034 to 0.0039 mg/(kg·year)and 0.343 to 0.391 mg/(kg·year),respectively,and were similar across the three cities,while ac-cumulation rates of Zn and Pb in Shanghai were higher than those in Shenzhen and Beijing.The higher accumulation rates of Zn and Pb in Shanghai can be explained by differences in city history and industrial structures among the cities.Residential soils with high health risks posed by the heavy metals were mostly collected from old towns of Shanghai because of high Pb content in the areas.Although recent urbanization resulted in elevated concen-trations of Cd,Cu,Zn,and Pb in the residential soils,the effect on the total health risks of residents exposed to the soils was negligible.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82172196(to KX),82372507(to KX)the Natural Science Foundation of Hunan Province,China,No.2023JJ40804(to QZ)the Key Laboratory of Emergency and Trauma(Hainan Medical University)of the Ministry of Education,China,No.KLET-202210(to QZ)。
文摘Ischemia–reperfusion injury is a common pathophysiological mechanism in retinal degeneration.PANoptosis is a newly defined integral form of regulated cell death that combines the key features of pyroptosis,apoptosis,and necroptosis.Oligomerization of mitochondrial voltage-dependent anion channel 1 is an important pathological event in regulating cell death in retinal ischemia–reperfusion injury.However,its role in PANoptosis remains largely unknown.In this study,we demonstrated that voltage-dependent anion channel 1 oligomerization-mediated mitochondrial dysfunction was associated with PANoptosis in retinal ischemia–reperfusion injury.Inhibition of voltage-dependent anion channel 1 oligomerization suppressed mitochondrial dysfunction and PANoptosis in retinal cells subjected to ischemia–reperfusion injury.Mechanistically,mitochondria-derived reactive oxygen species played a central role in the voltagedependent anion channel 1-mediated regulation of PANoptosis by promoting PANoptosome assembly.Moreover,inhibiting voltage-dependent anion channel 1 oligomerization protected against PANoptosis in the retinas of rats subjected to ischemia–reperfusion injury.Overall,our findings reveal the critical role of voltage-dependent anion channel 1 oligomerization in regulating PANoptosis in retinal ischemia–reperfusion injury,highlighting voltage-dependent anion channel 1 as a promising therapeutic target.
基金We acknowledge the financial support provided by the National Natural Science Foundation of China(No.41771532).
文摘Heavy metal concentrations in urban soils are likely to increase over time because of contin-uous urbanization and heavy metal emissions.To estimate the accumulation rates of heavy metals in urban soils,we collected soil samples from residential areas with different build-ing ages in the metropolitan cities of Shanghai,Shenzhen,and Beijing,China.Heavy metal concentrations in the soils varied among the cities and were primarily affected by soil parent material and the intensity of anthropogenic sources.Regression analyses revealed that the accumulation rates of Cd and Cu in the soils ranged from 0.0034 to 0.0039 mg/(kg·year)and 0.343 to 0.391 mg/(kg·year),respectively,and were similar across the three cities,while ac-cumulation rates of Zn and Pb in Shanghai were higher than those in Shenzhen and Beijing.The higher accumulation rates of Zn and Pb in Shanghai can be explained by differences in city history and industrial structures among the cities.Residential soils with high health risks posed by the heavy metals were mostly collected from old towns of Shanghai because of high Pb content in the areas.Although recent urbanization resulted in elevated concen-trations of Cd,Cu,Zn,and Pb in the residential soils,the effect on the total health risks of residents exposed to the soils was negligible.
