Since the evolving standardization of cellular V2X(C-V2X)technologies is continuously developed by 3GPP,research on new radio(NR-V2X)has been on schedule by academic and industries.Though millimeter wave(mm Wave)frequ...Since the evolving standardization of cellular V2X(C-V2X)technologies is continuously developed by 3GPP,research on new radio(NR-V2X)has been on schedule by academic and industries.Though millimeter wave(mm Wave)frequency band is supposed to provide large transmission bandwidth,yet the development of mm Wave band in NR-V2X is still in preliminary stage.In this article,a comprehensive discussion of mm Wave NR-V2X is given covering trends,standardization landscape,and enabling technologies,aiming at tackling the challenge of channel modeling,directional channel access,beamforming and blockage management.The vision of mm Wave NR-V2X is to fully support the development of automated driving,holographic control display and improved in-car infotainment for the future.展开更多
Objective:To analyze the clinical effect of laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors.Methods:A total of 100 patients with gastric neuroe...Objective:To analyze the clinical effect of laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors.Methods:A total of 100 patients with gastric neuroendocrine tumors that were treated in the Affiliated Hospital of Chifeng University from January 2016 to March 2021 were selected as the research subjects・They were divided into two groups by the digital table method.The control group underwent laparoscopic partial gastrectomy while the research group underwent laparoscopy combined with intraoperative gastroscopic partial gastrectomy.The curative effects of the two groups were compared.Results:There was no significant difference in the number of surgical lymph node dissections between the two groups(p>0.05);the amount of bleeding in the research group was lower than that in the control group;the operation time,time taken to get out of bed,gastrointestinal fimction recovery time,time taken for first eating,and hospital stay were shorter than those in the control group(p<0.05);the incidence of complications and the recurrence rate in the research group were lower than those in the control group(p<0.05).Conclusion:Laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors has significant clinical effect and high safety.展开更多
Single atom catalysts(SACs)have garnered significant attention in the field of catalysis over the past decade due to their exceptional atom utilization efficiency and distinct physical and chemical properties.For the ...Single atom catalysts(SACs)have garnered significant attention in the field of catalysis over the past decade due to their exceptional atom utilization efficiency and distinct physical and chemical properties.For the semiconductor-based electrical gas sensor,the core is the catalysis process of target gas molecules on the sensitive materials.In this context,the SACs offer great potential for highly sensitive and selective gas sensing,however,only some of the bubbles come to the surface.To facilitate practical applications,we present a comprehensive review of the preparation strategies for SACs,with a focus on overcoming the challenges of aggregation and low loading.Extensive research efforts have been devoted to investigating the gas sensing mechanism,exploring sensitive materials,optimizing device structures,and refining signal post-processing techniques.Finally,the challenges and future perspectives on the SACs based gas sensing are presented.展开更多
The importance of continuous healthcare management has significantly accelerated the development of wearable devices for monitoring health-related physical and biochemical markers. Despite extensive research on wearab...The importance of continuous healthcare management has significantly accelerated the development of wearable devices for monitoring health-related physical and biochemical markers. Despite extensive research on wearable devices for physiological and biochemical monitoring, critical issues of power management and device/skin interfacial properties restrict the advancement of personalized healthcare and early disease detection. Here, we report a multimodal sweat monitoring device featuring a real-time display and long-term data analysis based on self-powered format of sweat-activated batteries (SABs). The polyvinyl alcohol-sucrose (PVA-Suc) hydrogel serves as the key component for the SAB, offering not only great long-term adhesive properties for conformable wearability but also significant power generation capabilities. A maximum current density of 44.06 mA cm^(−2) and a maximum power density of 21.89 mW cm^(−2) can be realized for the hydrogel based SAB. The resulting device integrates an advanced colorimetric and electrochemical sensor array to measure pH levels, glucose concentrations, and chloride ion levels in human sweat, with data wirelessly transmitted by Near Field Communication. The self-powering features and multiple mode sensing function offer sufficient power to support real-time monitoring of metabolic biomarkers in sweat, with the ability to visually observe changes in the colorimetric sensors for long-term data monitoring.展开更多
In order to reduce the interference,a novel,cluster-based medium access control(MAC)protocol with load aware for VANETs is proposed in this paper.First,all vehicles on roads are grouped into stable clusters in the lig...In order to reduce the interference,a novel,cluster-based medium access control(MAC)protocol with load aware for VANETs is proposed in this paper.First,all vehicles on roads are grouped into stable clusters in the light of their direction,number of neighbors,link reliability,and traffic load.By utilizing the advantages of centralized control in software defined VANETs(SDVN),cluster stability can be maintained in real-time.Second,a contention-free MAC mechanism composed of inter-cluster multi-channel allocation and intra-cluster dynamic TDMA frame allocation is proposed to prevent co-channel interference and hidden terminal interference.Simulation results show that the proposed protocol outperforms some existing protocols in cluster stability,delivery ratio,throughput and delay performance.展开更多
The advent of Big Data has rendered Machine Learning tasks more intricate as they frequently involve higher-dimensional data.Feature Selection(FS)methods can abate the complexity of the data and enhance the accuracy,g...The advent of Big Data has rendered Machine Learning tasks more intricate as they frequently involve higher-dimensional data.Feature Selection(FS)methods can abate the complexity of the data and enhance the accuracy,generalizability,and interpretability of models.Meta-heuristic algorithms are often utilized for FS tasks due to their low requirements and efficient performance.This paper introduces an augmented Forensic-Based Investigation algorithm(DCFBI)that incorporates a Dynamic Individual Selection(DIS)and crisscross(CC)mechanism to improve the pursuit phase of the FBI.Moreover,a binary version of DCFBI(BDCFBI)is applied to FS.Experiments conducted on IEEE CEC 2017 with other metaheuristics demonstrate that DCFBI surpasses them in search capability.The influence of different mechanisms on the original FBI is analyzed on benchmark functions,while its scalability is verified by comparing it with the original FBI on benchmarks with varied dimensions.BDCFBI is then applied to 18 real datasets from the UCI machine learning database and the Wieslaw dataset to select near-optimal features,which are then compared with six renowned binary metaheuristics.The results show that BDCFBI can be more competitive than similar methods and acquire a subset of features with superior classification accuracy.展开更多
In this study, the transmission capacity of VANETs in a highway scenario is analysed on the basis of a 1D line model and the carrier sense multiple access with collision avoidance(CSMA/CA) protocol. We describe the CS...In this study, the transmission capacity of VANETs in a highway scenario is analysed on the basis of a 1D line model and the carrier sense multiple access with collision avoidance(CSMA/CA) protocol. We describe the CSMA/CA protocol used in IEEE802.11 p from the perspective of the geometric relationship amongst simultaneous transmitters. The desired channel and interfering channels are assumed to experience the same amount of path-loss and Rayleigh fading. On the basis of the proposed model, we analyse the attempted transmission probability of each road segment and the maximum intensity of active transmitters, including their theoretical values. Then, we employ the physical model to obtain the outage probability and derive the upper bound of the transmission capacity of a VANET, which is defined as the average spatial density of successful transmissions in the network. Simulation results indicate that the theoretical value offers a good bound on network capacity.展开更多
Hadal trenches are characterized by enhanced and infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter...Hadal trenches are characterized by enhanced and infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter the subseafloor microbiosphere.Currently,the role of high-rate episodic sedimentation in controlling the composition of the hadal subseafloor microbiosphere is unknown.Here,analyses of carbon isotope composition in a~750 cm long sediment core from the Challenger Deep revealed noncontinuous deposition,with anomalous ^(14)C ages likely caused by seismically driven mass transport and the funneling effect of trench geomorphology.Microbial community composition and diverse enzyme activities in the upper~27 cm differed from those at lower depths,probably due to sudden sediment deposition and differences in redox condition and organic matter availability.At lower depths,microbial population numbers,and composition remained relatively constant,except at some discrete depths with altered enzyme activity and microbial phyla abundance,possibly due to additional sudden sedimentation events of different magnitude.Evidence is provided of a unique role for high-rate episodic sedimentation events in controlling the subsurface microbiosphere in Earth’s deepest ocean floor and highlight the need to perform thorough analysis over a large depth range to characterize hadal benthic populations.Such depositional processes are likely crucial in shaping deep-water geochemical environments and thereby the deep subseafloor biosphere.展开更多
Background:Long non-coding ribonucleic acids(lncRNAs)regulate messenger RNA(mRNA)expression and influence cancer development and progression.Cuproptosis,a newly discovered form of cell death,plays an important role in...Background:Long non-coding ribonucleic acids(lncRNAs)regulate messenger RNA(mRNA)expression and influence cancer development and progression.Cuproptosis,a newly discovered form of cell death,plays an important role in cancer.Nonetheless,additional research investigating the association between cuproptosisrelated lncRNAs and prostate cancer(PCa)prognosis is required.Methods:Sequencing data and copy number variant data were obtained from 492 patients with PCa from The Cancer Genome Atlas(TCGA)Program.Prognostic models of PCa based on cuproptosis-related lncRNAs were constructed using a multi-level attention graph neural network(MLA-GNN)deep learning algorithm.Immune escape scoring was performed using Tumor Immune Dysfunction and Exclusion.Cellular experiments were conducted to explore the correlation between key lncRNAs and cuproptosis.Results:Data from 492 patients with PCa were randomized into two groups at a 1:1 ratio.Prognostic modeling was successfully established using MLA-GNN.Survival analysis suggested that patients could be divided into high-and low-risk groups according to model scores and that there was a significant difference in disease-free survival(DFS)(P<0.01).The area under the receiver operating characteristic(ROC)curve(AUC)indicated a strong predictive performance for the model,with AUCs of 0.913,0.847,and 0.863 for the training group and 0.815,0.907,and 0.866 for the test group at 12,36,and 60 months,respectively.The immune escape score and immune microenvironment analysis suggested that the high-risk group corresponded to a stronger immune escape and a poorer immune microenvironment(P<0.05).Cellular experiments revealed that the expression of all six key lncRNAs was upregulated in the presence of copper ion carriers(P<0.05).Conclusions:This study identified cuproptosis-related lncRNAs that were strongly associated with PCa prognosis.Key lncRNAs could affect copper metabolism and may serve as new therapeutic targets.展开更多
Background:Revealing the variations in soil aggregate-related organic carbon(OC)and labile organic carbon(LOC)fractions in a chronosequence of Chinese fir plantations plays an important role in better understanding th...Background:Revealing the variations in soil aggregate-related organic carbon(OC)and labile organic carbon(LOC)fractions in a chronosequence of Chinese fir plantations plays an important role in better understanding the impact of soil carbon sink or source on the Chinese fir plantation ecosystem.In this study,soil samples in a depth of 0–20 cm were collected from Chinese fir plantations at different stand ages(0,9,17,and 26 years old)in Guangxi,China.With the optimal moisture sieving method adopted,the soil aggregates of 4 different sizes were obtained,including>2-mm,2–1-mm,1–0.25-mm,and<0.25-mm aggregates.Soil OC and LOC fractions were measured in the aggregates of different sizes.The LOC fractions included readily oxidizable carbon(ROC),particulate organic carbon(POC),microbial biomass carbon(MBC),water-soluble organic carbon(WOC),and mineralized organic carbon(MOC).Results:Soil aggregate stability,as indicated by the mean weight diameter(MWD),was the highest in the 17-yearold Chinese fir plantations and was significantly positively related(p<0.05)to the concentrations of OC and LOC fractions(except for the ROC and MOC),with the POC in particular.As for all stand ages of Chinese fir plantations,the concentrations of soil OC and LOC fractions were significantly increased as the aggregate size decreased.Consequently,there were more OC and LOC fractions distributed in the<0.25-mm aggregates.During the stand development,the concentrations of soil OC and LOC fractions first increased and then decreased,with the highest levels detected in the 17-year-old Chinese fir plantations,indicating that the 17-year-old Chinese fir plantations were conducive to the accumulation of soil OC and LOC fractions.Conclusion:After 17 years of planting,promoted soil carbon(especially for the POC)accumulation contributes significantly to enhancing soil aggregate stability for the Chinese fir plantations in Guangxi,China.展开更多
Vibrio harveyi,which belongs to family Vibrionaceae of class G cunmap rote obacte ria,includes the species V.car char iae and V.trachuri as its junior synonyms.The organism is a well-recognized and serious bacterial p...Vibrio harveyi,which belongs to family Vibrionaceae of class G cunmap rote obacte ria,includes the species V.car char iae and V.trachuri as its junior synonyms.The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates,including penaeid shrimp,in aquaculture.Diseased fish may exhibit a range of lesions,including eye lesions/blindness,gastro-enteritis,muscle necrosis,skin ulcers,and tail rot disease.In shrimp,V.harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark.There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue.It is recognized that the pathogenicity mechanisms of V.harveyi may be different in fish and penaeid shrimp.In shrimp,the pathogenicity mechanisms involved the endotoxin lipopolysaccharide,and extracellular proteases,and interaction with bacteriophages.In fish,the pathogenicity mechanisms involved extracellular hemolysin(encoded by duplicate hemolysin genes),which was identified as a phospholipase B and could inactivate fish cells by apoptosis,via the caspase activation pathway.V.harveyi may enter the so-called viable but nonculturable(VBNC)state,and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture.Disease control measures center on dietary supplements(including probiotics),nonspecific immunostimulants,and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.展开更多
The spectrum scarcity of VANETs(Vehicular Ad hoc Networks)can be alleviated by spectrum sharing technology.We present a framework of CCR-VANETs(Cellular-based Cognitive-radio Vehicular Ad hoc Networks).In CCR-VANETs,c...The spectrum scarcity of VANETs(Vehicular Ad hoc Networks)can be alleviated by spectrum sharing technology.We present a framework of CCR-VANETs(Cellular-based Cognitive-radio Vehicular Ad hoc Networks).In CCR-VANETs,cellular network performs as primary network while VANET shares the downlink spectrum of cellular network.We consider a scalable urban grid scenario in which vehicles detect available spectrum holes and opportunistically access them according to a carrier-sensing multiple-access protocol.To restrict vehicles'interference to primary receivers,we set a square preservation region around each particular street block where an active base station is located.The number of street blocks in the preservation region is calculated with the practical assumption that vehicles only know the locations of primary transmitters.We analyze the aggregate interference power from primary and secondary networks,then derive the lower-bound of downlink capacity for the primary network and lower-bound of V2V(Vehicle-to-Vehicle)channel capacity for the secondary network respectively.The numerical results demonstrate the impacts of di erent network parameters on inter-networks interference level and network capacities.展开更多
基金supported by the National Key Research and Development Program of China(2020YFB1807900)the National Natural Science Foundation of China(61931005,62001051)。
文摘Since the evolving standardization of cellular V2X(C-V2X)technologies is continuously developed by 3GPP,research on new radio(NR-V2X)has been on schedule by academic and industries.Though millimeter wave(mm Wave)frequency band is supposed to provide large transmission bandwidth,yet the development of mm Wave band in NR-V2X is still in preliminary stage.In this article,a comprehensive discussion of mm Wave NR-V2X is given covering trends,standardization landscape,and enabling technologies,aiming at tackling the challenge of channel modeling,directional channel access,beamforming and blockage management.The vision of mm Wave NR-V2X is to fully support the development of automated driving,holographic control display and improved in-car infotainment for the future.
文摘Objective:To analyze the clinical effect of laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors.Methods:A total of 100 patients with gastric neuroendocrine tumors that were treated in the Affiliated Hospital of Chifeng University from January 2016 to March 2021 were selected as the research subjects・They were divided into two groups by the digital table method.The control group underwent laparoscopic partial gastrectomy while the research group underwent laparoscopy combined with intraoperative gastroscopic partial gastrectomy.The curative effects of the two groups were compared.Results:There was no significant difference in the number of surgical lymph node dissections between the two groups(p>0.05);the amount of bleeding in the research group was lower than that in the control group;the operation time,time taken to get out of bed,gastrointestinal fimction recovery time,time taken for first eating,and hospital stay were shorter than those in the control group(p<0.05);the incidence of complications and the recurrence rate in the research group were lower than those in the control group(p<0.05).Conclusion:Laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors has significant clinical effect and high safety.
基金supported by the National Key Research and Development Program of China(2022YFB3204700)the National Natural Science Foundation of China(52122513)+2 种基金the Natural Science Foundation of Heilongjiang Province(YQ2021E022)the Natural Science Foundation of Chongqing(2023NSCQ-MSX2286)the Fundamental Research Funds for the Central Universities(HIT.BRET.2021010)。
文摘Single atom catalysts(SACs)have garnered significant attention in the field of catalysis over the past decade due to their exceptional atom utilization efficiency and distinct physical and chemical properties.For the semiconductor-based electrical gas sensor,the core is the catalysis process of target gas molecules on the sensitive materials.In this context,the SACs offer great potential for highly sensitive and selective gas sensing,however,only some of the bubbles come to the surface.To facilitate practical applications,we present a comprehensive review of the preparation strategies for SACs,with a focus on overcoming the challenges of aggregation and low loading.Extensive research efforts have been devoted to investigating the gas sensing mechanism,exploring sensitive materials,optimizing device structures,and refining signal post-processing techniques.Finally,the challenges and future perspectives on the SACs based gas sensing are presented.
基金Research Grants Council,University Grants Committee,Grant/Award Numbers:11211523,11213721,11215722,RFS2324-1S03National Natural Science Foundation of China,Grant/Award Number:62122002City University of Hong Kong,Grant/Award Numbers:9667199,9667221,9667246,9680322。
文摘The importance of continuous healthcare management has significantly accelerated the development of wearable devices for monitoring health-related physical and biochemical markers. Despite extensive research on wearable devices for physiological and biochemical monitoring, critical issues of power management and device/skin interfacial properties restrict the advancement of personalized healthcare and early disease detection. Here, we report a multimodal sweat monitoring device featuring a real-time display and long-term data analysis based on self-powered format of sweat-activated batteries (SABs). The polyvinyl alcohol-sucrose (PVA-Suc) hydrogel serves as the key component for the SAB, offering not only great long-term adhesive properties for conformable wearability but also significant power generation capabilities. A maximum current density of 44.06 mA cm^(−2) and a maximum power density of 21.89 mW cm^(−2) can be realized for the hydrogel based SAB. The resulting device integrates an advanced colorimetric and electrochemical sensor array to measure pH levels, glucose concentrations, and chloride ion levels in human sweat, with data wirelessly transmitted by Near Field Communication. The self-powering features and multiple mode sensing function offer sufficient power to support real-time monitoring of metabolic biomarkers in sweat, with the ability to visually observe changes in the colorimetric sensors for long-term data monitoring.
文摘In order to reduce the interference,a novel,cluster-based medium access control(MAC)protocol with load aware for VANETs is proposed in this paper.First,all vehicles on roads are grouped into stable clusters in the light of their direction,number of neighbors,link reliability,and traffic load.By utilizing the advantages of centralized control in software defined VANETs(SDVN),cluster stability can be maintained in real-time.Second,a contention-free MAC mechanism composed of inter-cluster multi-channel allocation and intra-cluster dynamic TDMA frame allocation is proposed to prevent co-channel interference and hidden terminal interference.Simulation results show that the proposed protocol outperforms some existing protocols in cluster stability,delivery ratio,throughput and delay performance.
基金supported by Special Fund of Fundamental Scientific Research Business Expense for Higher School of Central Government(ZY20180119)the Natural Science Foundation of Zhejiang Province(LZ22F020005)+1 种基金the Natural Science Foundation of Hebei Province(D2022512001)National Natural Science Foundation of China(42164002,62076185).
文摘The advent of Big Data has rendered Machine Learning tasks more intricate as they frequently involve higher-dimensional data.Feature Selection(FS)methods can abate the complexity of the data and enhance the accuracy,generalizability,and interpretability of models.Meta-heuristic algorithms are often utilized for FS tasks due to their low requirements and efficient performance.This paper introduces an augmented Forensic-Based Investigation algorithm(DCFBI)that incorporates a Dynamic Individual Selection(DIS)and crisscross(CC)mechanism to improve the pursuit phase of the FBI.Moreover,a binary version of DCFBI(BDCFBI)is applied to FS.Experiments conducted on IEEE CEC 2017 with other metaheuristics demonstrate that DCFBI surpasses them in search capability.The influence of different mechanisms on the original FBI is analyzed on benchmark functions,while its scalability is verified by comparing it with the original FBI on benchmarks with varied dimensions.BDCFBI is then applied to 18 real datasets from the UCI machine learning database and the Wieslaw dataset to select near-optimal features,which are then compared with six renowned binary metaheuristics.The results show that BDCFBI can be more competitive than similar methods and acquire a subset of features with superior classification accuracy.
基金supported in part by the National Natural Science Foundation of China under Grant No.61271184 and 61571065
文摘In this study, the transmission capacity of VANETs in a highway scenario is analysed on the basis of a 1D line model and the carrier sense multiple access with collision avoidance(CSMA/CA) protocol. We describe the CSMA/CA protocol used in IEEE802.11 p from the perspective of the geometric relationship amongst simultaneous transmitters. The desired channel and interfering channels are assumed to experience the same amount of path-loss and Rayleigh fading. On the basis of the proposed model, we analyse the attempted transmission probability of each road segment and the maximum intensity of active transmitters, including their theoretical values. Then, we employ the physical model to obtain the outage probability and derive the upper bound of the transmission capacity of a VANET, which is defined as the average spatial density of successful transmissions in the network. Simulation results indicate that the theoretical value offers a good bound on network capacity.
基金supported by the Laoshan laboratory(LSKJ202203206)National Natural Science Foundation of China(92051115 and 42230412)+1 种基金the Fundamental Research Funds for the Central Universities(202172002 and 202141009)the Shandong Province Natural Science Foundation(ZR2022YQ38).
文摘Hadal trenches are characterized by enhanced and infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter the subseafloor microbiosphere.Currently,the role of high-rate episodic sedimentation in controlling the composition of the hadal subseafloor microbiosphere is unknown.Here,analyses of carbon isotope composition in a~750 cm long sediment core from the Challenger Deep revealed noncontinuous deposition,with anomalous ^(14)C ages likely caused by seismically driven mass transport and the funneling effect of trench geomorphology.Microbial community composition and diverse enzyme activities in the upper~27 cm differed from those at lower depths,probably due to sudden sediment deposition and differences in redox condition and organic matter availability.At lower depths,microbial population numbers,and composition remained relatively constant,except at some discrete depths with altered enzyme activity and microbial phyla abundance,possibly due to additional sudden sedimentation events of different magnitude.Evidence is provided of a unique role for high-rate episodic sedimentation events in controlling the subsurface microbiosphere in Earth’s deepest ocean floor and highlight the need to perform thorough analysis over a large depth range to characterize hadal benthic populations.Such depositional processes are likely crucial in shaping deep-water geochemical environments and thereby the deep subseafloor biosphere.
基金supported by grants from the National Natural Science Foundation of China(Nos.82372912,81802527,81972471,82073408,81974395,and 82173036)National Key Research and Development Program of China(Nos.2022YFC3602900 and 2022YFC3602904)+13 种基金BeijingBethune CharitableFoundation(Nos.mnzl202001 and mnzl202026)Guangzhou Science and Technology Project(Nos.202206010078 and 202201020574)Sun Yat-sen University Clinical Research 5010 Program(Nos.2018007 and 2019005)Sun Yat-sen Clinical Research Cultivating Program(No.SYS-C-201801)Guangdong Medical Science and Technology Program(No.A2020558)Tencent Charity Foundation(No.7670020025)Scientific Research Launch Project of Sun Yat-sen Memorial Hospital(No.YXQH202209)GuangdongBasicand AppliedBasicResearchFoundation(No.2019A1515011437)International Science and Technology Cooperation Project Plan of Guangdong Province(No.2021A0505030085)Guangzhou Science and Technology Key R&D Project(No.202206010117)Beijing CSCO Clinical Oncology Research Foundation(No.Y-tongshu2021/ms-0162)Guangdong Provincial Clinical Research Center for Urological Diseases(No.2020B1111170006)Guangdong Science and Technology Department(No.2020B1212060018)open research funds from the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital.
文摘Background:Long non-coding ribonucleic acids(lncRNAs)regulate messenger RNA(mRNA)expression and influence cancer development and progression.Cuproptosis,a newly discovered form of cell death,plays an important role in cancer.Nonetheless,additional research investigating the association between cuproptosisrelated lncRNAs and prostate cancer(PCa)prognosis is required.Methods:Sequencing data and copy number variant data were obtained from 492 patients with PCa from The Cancer Genome Atlas(TCGA)Program.Prognostic models of PCa based on cuproptosis-related lncRNAs were constructed using a multi-level attention graph neural network(MLA-GNN)deep learning algorithm.Immune escape scoring was performed using Tumor Immune Dysfunction and Exclusion.Cellular experiments were conducted to explore the correlation between key lncRNAs and cuproptosis.Results:Data from 492 patients with PCa were randomized into two groups at a 1:1 ratio.Prognostic modeling was successfully established using MLA-GNN.Survival analysis suggested that patients could be divided into high-and low-risk groups according to model scores and that there was a significant difference in disease-free survival(DFS)(P<0.01).The area under the receiver operating characteristic(ROC)curve(AUC)indicated a strong predictive performance for the model,with AUCs of 0.913,0.847,and 0.863 for the training group and 0.815,0.907,and 0.866 for the test group at 12,36,and 60 months,respectively.The immune escape score and immune microenvironment analysis suggested that the high-risk group corresponded to a stronger immune escape and a poorer immune microenvironment(P<0.05).Cellular experiments revealed that the expression of all six key lncRNAs was upregulated in the presence of copper ion carriers(P<0.05).Conclusions:This study identified cuproptosis-related lncRNAs that were strongly associated with PCa prognosis.Key lncRNAs could affect copper metabolism and may serve as new therapeutic targets.
基金The National Natural Science Foundation of China(No.31460196)Research Basic Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities(No.2021KY0014)financed the present study。
文摘Background:Revealing the variations in soil aggregate-related organic carbon(OC)and labile organic carbon(LOC)fractions in a chronosequence of Chinese fir plantations plays an important role in better understanding the impact of soil carbon sink or source on the Chinese fir plantation ecosystem.In this study,soil samples in a depth of 0–20 cm were collected from Chinese fir plantations at different stand ages(0,9,17,and 26 years old)in Guangxi,China.With the optimal moisture sieving method adopted,the soil aggregates of 4 different sizes were obtained,including>2-mm,2–1-mm,1–0.25-mm,and<0.25-mm aggregates.Soil OC and LOC fractions were measured in the aggregates of different sizes.The LOC fractions included readily oxidizable carbon(ROC),particulate organic carbon(POC),microbial biomass carbon(MBC),water-soluble organic carbon(WOC),and mineralized organic carbon(MOC).Results:Soil aggregate stability,as indicated by the mean weight diameter(MWD),was the highest in the 17-yearold Chinese fir plantations and was significantly positively related(p<0.05)to the concentrations of OC and LOC fractions(except for the ROC and MOC),with the POC in particular.As for all stand ages of Chinese fir plantations,the concentrations of soil OC and LOC fractions were significantly increased as the aggregate size decreased.Consequently,there were more OC and LOC fractions distributed in the<0.25-mm aggregates.During the stand development,the concentrations of soil OC and LOC fractions first increased and then decreased,with the highest levels detected in the 17-year-old Chinese fir plantations,indicating that the 17-year-old Chinese fir plantations were conducive to the accumulation of soil OC and LOC fractions.Conclusion:After 17 years of planting,promoted soil carbon(especially for the POC)accumulation contributes significantly to enhancing soil aggregate stability for the Chinese fir plantations in Guangxi,China.
基金The work was supported by the National Key Research and Development Program of China(No.2018YFE0124100)the National Natural Science Foundation of China(Nos.41730530 and 91751202)Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0406-4).
文摘Vibrio harveyi,which belongs to family Vibrionaceae of class G cunmap rote obacte ria,includes the species V.car char iae and V.trachuri as its junior synonyms.The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates,including penaeid shrimp,in aquaculture.Diseased fish may exhibit a range of lesions,including eye lesions/blindness,gastro-enteritis,muscle necrosis,skin ulcers,and tail rot disease.In shrimp,V.harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark.There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue.It is recognized that the pathogenicity mechanisms of V.harveyi may be different in fish and penaeid shrimp.In shrimp,the pathogenicity mechanisms involved the endotoxin lipopolysaccharide,and extracellular proteases,and interaction with bacteriophages.In fish,the pathogenicity mechanisms involved extracellular hemolysin(encoded by duplicate hemolysin genes),which was identified as a phospholipase B and could inactivate fish cells by apoptosis,via the caspase activation pathway.V.harveyi may enter the so-called viable but nonculturable(VBNC)state,and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture.Disease control measures center on dietary supplements(including probiotics),nonspecific immunostimulants,and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.
基金This work is supported in part by the National Natural Science Foundation of China(Nos.61271184,61571065).
文摘The spectrum scarcity of VANETs(Vehicular Ad hoc Networks)can be alleviated by spectrum sharing technology.We present a framework of CCR-VANETs(Cellular-based Cognitive-radio Vehicular Ad hoc Networks).In CCR-VANETs,cellular network performs as primary network while VANET shares the downlink spectrum of cellular network.We consider a scalable urban grid scenario in which vehicles detect available spectrum holes and opportunistically access them according to a carrier-sensing multiple-access protocol.To restrict vehicles'interference to primary receivers,we set a square preservation region around each particular street block where an active base station is located.The number of street blocks in the preservation region is calculated with the practical assumption that vehicles only know the locations of primary transmitters.We analyze the aggregate interference power from primary and secondary networks,then derive the lower-bound of downlink capacity for the primary network and lower-bound of V2V(Vehicle-to-Vehicle)channel capacity for the secondary network respectively.The numerical results demonstrate the impacts of di erent network parameters on inter-networks interference level and network capacities.
