To address the challenge of missing modal information in entity alignment and to mitigate information loss or bias arising frommodal heterogeneity during fusion,while also capturing shared information acrossmodalities...To address the challenge of missing modal information in entity alignment and to mitigate information loss or bias arising frommodal heterogeneity during fusion,while also capturing shared information acrossmodalities,this paper proposes a Multi-modal Pre-synergistic Entity Alignmentmodel based on Cross-modalMutual Information Strategy Optimization(MPSEA).The model first employs independent encoders to process multi-modal features,including text,images,and numerical values.Next,a multi-modal pre-synergistic fusion mechanism integrates graph structural and visual modal features into the textual modality as preparatory information.This pre-fusion strategy enables unified perception of heterogeneous modalities at the model’s initial stage,reducing discrepancies during the fusion process.Finally,using cross-modal deep perception reinforcement learning,the model achieves adaptive multilevel feature fusion between modalities,supporting learningmore effective alignment strategies.Extensive experiments on multiple public datasets show that the MPSEA method achieves gains of up to 7% in Hits@1 and 8.2% in MRR on the FBDB15K dataset,and up to 9.1% in Hits@1 and 7.7% in MRR on the FBYG15K dataset,compared to existing state-of-the-art methods.These results confirm the effectiveness of the proposed model.展开更多
In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undat...In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undatus (Haw.) Britton & Rose (H. undatus) has not been reported yet. Through transcriptomic analysis, virus-induced gene silencing (VIGS) technology, and RT-qPCR validation, we investigated the role of the LBD TFs in the senescence of H. undatus. Transcriptomic analysis revealed that HuLBD1 is a key transcription factor of the LBD family regulating H. undatus senescence. After silencing HuLBD1, 5075 differentially expressed genes (DEGs) were identified. GSEA results showed that these DEGs were mainly enriched in the phenylpropanoids and flavonoid biosynthesis pathway. The leading subset of genes in these two pathways included 16 DEGs, with four upregulated genes, HuCHS1-2, HuFLS1, HuCYP75B2, and HuCHS5-2 being central in the Protein-Protein Interaction (PPI) network. H. undatus phenotypic experiments confirmed that silencing HuLBD1 significantly increased the flavonoid content in the fruit peel, and RT-qPCR validation showed that the gene expression trends were consistent with the RNA-seq data. The study indicated that HuLBD1 likely delays the fruit senescence process by negatively regulating the expression of four key genes, including HuCHS1-2, thus promoting the synthesis of phenylpropanoids such as flavonoids. In conclusion, our findings suggest that the HuLBD1 TF plays an important role in the senescence of H. undatus fruit, providing a theoretical basis for postharvest senescence control.展开更多
The development of high-performance layered oxide cathodes for sodium ion batteries (SIBs) continues to facing be hindered by severe challenges to date.Herein,a single-phase P2-Na0.67Mn0.6Ni0.2Co0.1Cu0.1O2(NMNCC) comp...The development of high-performance layered oxide cathodes for sodium ion batteries (SIBs) continues to facing be hindered by severe challenges to date.Herein,a single-phase P2-Na0.67Mn0.6Ni0.2Co0.1Cu0.1O2(NMNCC) comprising multiple-layer-oriented stacked nanoflakes is designed and synthesized via a simple sol-gel method.The large lattice parameters ensure a large three-dimensional frame,which enables the diffusion of sodium ions.Owing to its optimal morphology structure modulation transition metal substitution strategy,the MNCC electrode delivers a reversible capacity of 131.3 mAh g^-1 at 0.1 C with retention of 86.7%after 200 cycles.In addition,it provides an initial capacity of 86.7 mAh g^-1,and a retention of 80.0%after 500 cycles even at a current density of up to 1 A g^-1.The stable single-phase structure and slight volume shrinkage observed after Na+extraction further delay structural degradation.High Na+mobility and low Na+diffusion resistance are also guarantee the excellent rate performance of the NMNCC electrode.Thus,we determine that the NMNCC cathode is significant in the advancement of promising novel layered oxide cathodes.展开更多
The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the result...The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the results of multi-center clinical nutrition research for congenital heart disease following thefirst Chinese consensus edition of 2016.The consensus was also shaped by the results of three discussion sessions and two questionnaires con-ducted by the 13-member collaboration group.This process was informed by both clinical guidelines and expert consensus.The quality of literature,both in English and Chinese,and the level of recommendations were evaluated using the Grading of Recommendations Assessment,Development,and Evaluations(GRADE)system.展开更多
African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the ...African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus(r NDV) expressing ASFV protein 72(p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, r NDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in r NDV/p72 was stably maintained through ten passages. Mice immunized with r NDV/p72 developed high titers of ASFV p72 specific Ig G antibody, and had higher levels of Ig G1 than IgG2 a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that r NDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.展开更多
Rheumatoid arthritis(RA)is an autoimmune disease.Early studies hold an opinion that gut microbiota is environmentally acquired and associated with RA susceptibility.However,accumulating evidence demonstrates that gene...Rheumatoid arthritis(RA)is an autoimmune disease.Early studies hold an opinion that gut microbiota is environmentally acquired and associated with RA susceptibility.However,accumulating evidence demonstrates that genetics also shape the gut microbiota.It is known that some strains of inbred laboratory mice are highly susceptible to collagen-induced arthritis(CIA),while the others are resistant to CIA.Here,we show that transplantation of fecal microbiota of CIA-resistant C57BL/6J mice to CIA-susceptible DBA/1J mice confer CIA resistance in DBA/1J mice.C57BL/6J mice and healthy human individuals have enriched B.fragilis than DBA/1J mice and RA patients.Transplantation of B.fragilis prevents CIA in DBA/1J mice.We identify that B.fragilis mainly produces propionate and C57BL/6J mice and healthy human individuals have higher level of propionate.Fibroblast-like synoviocytes(FLSs)in RA are activated to undergo tumor-like transformation.Propionate disrupts HDAC3-FOXK1 interaction to increase acetylation of FOXK1,resulting in reduced FOXK1 stability,blocked interferon signaling and deactivation of RA-FLSs.We treat CIA mice with propionate and show that propionate attenuates CIA.Moreover,a combination of propionate with anti-TNF etanercept synergistically relieves CIA.These results suggest that B.fragilis or propionate could be an alternative or complementary approach to the current therapies.展开更多
Climate change threatens China’s rice production,making it crucial to assess the impact of climate change and climate year type(CYT)on rice production across regions to safeguard food security.The impact of climate c...Climate change threatens China’s rice production,making it crucial to assess the impact of climate change and climate year type(CYT)on rice production across regions to safeguard food security.The impact of climate change under nine CYTs with different combinations of temperature and precipitation on two rice cropping systems,including single rice and double rice(early and late rice)was evaluated.The results indicate that:(1)the Northeast region was expected to undergo the greatest warming of 2.03–2.48℃,and future climate conditions would be dominated by Warm-Humid,Warm-Normal,and Warm-Dry CYTs across all regions.(2)Climate change would significantly shorten anthesis days after sowing and maturity days after sowing of single rice by 6–12 days and 9–24 days,with little change observed for late rice(<1 day).Late rice yield suffered more from climate change compared to single and early rice yield,declining by 8.8%–16.13%.(3)Different CYTs exhibited varying impacts on rice yields.Yields were projected to decrease by approximately 4.765%to 18.645%in Warm-Humid,Warm-Normal,and Warm-Dry CYTs.Conversely,the Northeast region was anticipated to experience an increase in yield.(4)Relationships between rice yield and meteorological factors varied by region,variety,and CYT.Among the nine CYTs,high killing degree days,mean daily temperature,mean solar radiation and warm spell duration index were the main factors influencing changes in rice yield,explaining nearly 80%of yield change.Our results would help to develop adaptation strategies in different regions and rice cropping systems.展开更多
Neurosyphilis(NS)is an infectious disease caused by Treponema pallidum invading the central nervous system.It can manifest at any stage of syphilis,and is often misdiagnosed due to its atypical and progressive symptom...Neurosyphilis(NS)is an infectious disease caused by Treponema pallidum invading the central nervous system.It can manifest at any stage of syphilis,and is often misdiagnosed due to its atypical and progressive symptoms.The increasing incidence of NS underscores the necessity for early and accurate diagnosis.Here,we present a case where routine cerebrospinal fluid metagenomic next-generation sequencing(mNGS)was used to diagnose a patient with neurosyphilis.The patient exhibited cognitive impairment and was initially diagnosed with cerebral infarction due to syphilitic cerebral arteritis.Thus,the patient was treated with dual antiplatelet therapy(aspirin and clopidogrel)and statins to stabilize the plaques.Neurosyphilis was treated with penicillin sodium injections,resulting in significant improvement in the patient’s mental state.This case is a rare instance of neurosyphilis associated with cerebral infarction.These findings suggest that mNGS is a valuable tool in diagnosing neurosyphilis,potentially improving diagnostic accuracy and patient outcomes.展开更多
Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites.Flavonoids are natural compounds that possess many important biological activities,playing a significant role in the medicina...Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites.Flavonoids are natural compounds that possess many important biological activities,playing a significant role in the medicinal activity of Eucommia ulmoides(E.ulmoides).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in E.ulmoides,a comprehensive analysis of metabolomics and transcriptomics was conducted.The results indicated that salt stress led to the wilting and darkening of E.ulmoides leaves,accompanied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical conductivity.During salt stress,most metabolites in the flavonoid biosynthesis pathway of E.ulmoides were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt stress.Among them,secondary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt stress.The correlation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as 6-Hydroxyluteolin.The co-expression network showed a strong positive correlation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative correlation with Catechin biosynthesis.The branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid compound biosynthesis in the plant stress response during salt stress.The results of this study provided a preliminary mechanism of secondary metabolites such as flavonoids in the medicinal plant E.ulmoides induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress response.It also provided useful information for subsequent exploration of resistance genes in E.ulmoides.展开更多
During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseflavonoid compound levels and reduce oxidative damage.To investigate the mechanism of trypsin-inducedflavonoid bio-...During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseflavonoid compound levels and reduce oxidative damage.To investigate the mechanism of trypsin-inducedflavonoid bio-synthesis in C.sativus,we conducted a combined analysis of transcriptomics and widely targeted metabolomics.One hundred and seventy-five significantly different metabolites were obtained from metabolomics data.The kyoto encyclopedia of genes and genomes(KEGG)functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway.By combining the results of the weighted gene co-expression network analysis(WGCNA)with the 130 upregulated phenylpropanoid metabolites,22 significantly upregulated phenylpropanoid metabolites were identified.Trilobatin was identified as the most prominent meta-bolite through cluster analysis and variable importance in projection(VIP)analysis.High performance liquid chro-matography(HPLC)experiments confirmed that trilobatin was the key metabolite induced by trypsin.The transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated withflavonoid biosynthesis.The gene set enrichment analysis(GSEA)identified leading edges in 4 key KEGG path-ways.Finally,combined with WGCNA and GSEA analysis results,35 core genes were obtained.The co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of trilobatin.The quantitative real-time polymerase chain reaction(RT-qPCR)and dual luciferase experiments con-firmed the activation effect of CsWRKY28 on CsMYC2 and downstream target genes.This study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in C.sativus and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.展开更多
Aims:Multiple genes and environmental factors are known to be involved in congenital heart disease(CHD),but epigenetic variation has received little attention.Monozygotic(MZ)twins with CHD provide a unique model for e...Aims:Multiple genes and environmental factors are known to be involved in congenital heart disease(CHD),but epigenetic variation has received little attention.Monozygotic(MZ)twins with CHD provide a unique model for exploring this phenomenon.In order to investigate the potential role of Deoxyribonucleic Acid(DNA)methyla-tion in CHD pathogenesis,the present study examined DNA methylation variation in MZ twins discordant for CHD,especially ventricular septal defect(VSD).Methods and Results:Using genome-wide DNA methylation profiles,we identified 4004 differentially methylated regions(DMRs)in 18 MZ twin pairs discordant for CHD,and 2826 genes were identified.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed a list of CHD-associated pathways.To further investigate the role of DNA methylation in VSD,data from 7 pairs of MZ twins with VSD were analyzed.We identified 1614 DMRs corresponding to 1443 genes associated with arrhythmogenic right ventricular cardiomyopathy,cyclic guanosine monopho-sphate-protein kinase G(cGMP-PKG)signaling pathway by KEGG analysis,and cell-cell adhesion,calcium ion transmembrane transport by GO analysis.A proportion of DMR-associated genes were involved in calcium signaling pathways.The methylation changes of calcium signaling genes might be related to VSD pathogenesis.Conclusion:CHD is associated with differential DNA methylation in MZ twins.CHD may be etiologically linked to DNA methylation,and methylation of calcium signaling genes may be involved in the development of VSD.展开更多
Objective:Limited data are available regarding current outcomes after revascularization for angiographically confirmed very late stent thrombosis(VLST).Therefore,this study investigated the current outcomes of patient...Objective:Limited data are available regarding current outcomes after revascularization for angiographically confirmed very late stent thrombosis(VLST).Therefore,this study investigated the current outcomes of patients with VLST and the risk factors for major adverse cardiovascular events(MACE).Methods:Consecutive patients who underwent revascularization for angiographically confirmed VLST between January 2014 and January 2016 were enrolled in this study.The patients were divided into two groups according to the occurrence of MACE during follow-up.The clinical and interventional parameters between groups were compared.Results:A total of 564 patients with an average age of 61.61±10.71 years were enrolled.Eight patients died during hospitalization.MACE occurred in 100 patients after a mean follow-up of 620 days.The total MACE rate was 35.46%,and Kaplan-Meier survival analysis indicated an estimated MACE-free survival rate of 22%.Multivariable Cox regression analysis revealed that left ventricular ejection fraction and peak troponin I at VLST onset were independent predictors of MACE.Conclusions:Long-term outcomes after revascularization for VLST remained unfavorable.Left ventricular ejection fraction and peak troponin I at the onset of VLST were independent risk factors for poor prognosis.展开更多
Gene transcription is a stochastic process characterized by fluctuations in mRNA levels of the same gene in isogenic cell populations.A central question in single-cell studies is how to map transcriptional variability...Gene transcription is a stochastic process characterized by fluctuations in mRNA levels of the same gene in isogenic cell populations.A central question in single-cell studies is how to map transcriptional variability to phenotypic differences between isogenic cells.We introduced a measurable and statistical transcription threshold I for critical genes that determine the entry level of Waddington’s canal toward a specific cell fate.Subsequently,J_(I),which is the probability that a cell has at least I mRNA molecules of a given gene,approximates the likelihood of a cell committing to the corresponding fate.In this study,we extended the previous results of J_(I) of the classical telegraph model by considering more complex models with different gene activation frameworks.We showed that(a)the upregulation of the critical gene may significantly suppress cell fate change and(b)increasing transcription noise performs a bidirectional role that can either enhance or suppress the cell fate change.These observations matched accurately with the data from bacterial,yeast,and mammalian cells.We estimated the threshold I from these data and predicted that(a)the traditional human immunodeficiency virus(HIV)activators that modulate gene activation frequency at high doses may largely suppress HIV reactivation and(b)the cells may favor noisier(or less noisy)regulation of stress genes under high(or low)environmental pressures to maintain cell viability.展开更多
Herein,we define the role of ferroptosis in the pathogenesis of diabetic cardiomyopathy(DCM)by examining the expression of key regulators of ferroptosis in mice with DCM and a new ex vivo DCM model.Advanced glycation ...Herein,we define the role of ferroptosis in the pathogenesis of diabetic cardiomyopathy(DCM)by examining the expression of key regulators of ferroptosis in mice with DCM and a new ex vivo DCM model.Advanced glycation end-products(AGEs),an important pathogenic factor of DCM,were found to induce ferroptosis in engineered cardiac tissues(ECTs),as reflected through increased levels of Ptgs2 and lipid peroxides and decreased ferritin and SLC7 A11 levels.Typical morphological changes of ferroptosis in cardiomyocytes were observed using transmission electron microscopy.Inhibition of ferroptosis with ferrostatin-1 and deferoxamine prevented AGE-induced ECT remodeling and dysfunction.Ferroptosis was also evidenced in the heart of type 2 diabetic mice with DCM.Inhibition of ferroptosis by liproxstatin-1 prevented the development of diastolic dysfunction at 3 months after the onset of diabetes.Nuclear factor erythroid 2-related factor 2(NRF2)activated by sulforaphane inhibited cardiac cell ferroptosis in both AGE-treated ECTs and hearts of DCM mice by upregulating ferritin and SLC7 A11 levels.The protective effect of sulforaphane on ferroptosis was AMP-activated protein kinase(AMPK)-dependent.These findings suggest that ferroptosis plays an essential role in the pathogenesis of DCM;sulforaphane prevents ferroptosis and associated pathogenesis via AMPK-mediated NRF2 activation.This suggests a feasible therapeutic approach with sulforaphane to clinically prevent ferroptosis and DCM.展开更多
A significant gap remains between rural and urban students in the rate of admission to senior high school. One reason for this gap might be the high levels of tuition and fees for senior high school By reducing stude...A significant gap remains between rural and urban students in the rate of admission to senior high school. One reason for this gap might be the high levels of tuition and fees for senior high school By reducing students＇ expectations of attending high school, high levels of tuition and fees could be reducing student aeademic performance in junior high schools. In this paper we evaluate the impacts of a senior high tuition relief program on the test scores of poor rural seventh grade students in China. We surveyed three counties in Shaanxi Province and exploited the fact that, while the counties are adjacent to one another and share similar characteristics, only one of the three implemented a tuition relief program. Using several alternative estimation strategies, including difference-in-differences, propensity score matching and difference-in-differences matching, we find that the tuition program has a statistically significant and positive impact on the mathematics scores of seventh grade students. More importantly, this program is shown to have a statistically significant and positive effect on the poorest students in the treatment group compared to their wealthier peers.展开更多
基金partially supported by the National Natural Science Foundation of China under Grants 62471493 and 62402257(for conceptualization and investigation)partially supported by the Natural Science Foundation of Shandong Province,China under Grants ZR2023LZH017,ZR2024MF066,and 2023QF025(for formal analysis and validation)+1 种基金partially supported by the Open Foundation of Key Laboratory of Computing Power Network and Information Security,Ministry of Education,Qilu University of Technology(Shandong Academy of Sciences)under Grant 2023ZD010(for methodology and model design)partially supported by the Russian Science Foundation(RSF)Project under Grant 22-71-10095-P(for validation and results verification).
文摘To address the challenge of missing modal information in entity alignment and to mitigate information loss or bias arising frommodal heterogeneity during fusion,while also capturing shared information acrossmodalities,this paper proposes a Multi-modal Pre-synergistic Entity Alignmentmodel based on Cross-modalMutual Information Strategy Optimization(MPSEA).The model first employs independent encoders to process multi-modal features,including text,images,and numerical values.Next,a multi-modal pre-synergistic fusion mechanism integrates graph structural and visual modal features into the textual modality as preparatory information.This pre-fusion strategy enables unified perception of heterogeneous modalities at the model’s initial stage,reducing discrepancies during the fusion process.Finally,using cross-modal deep perception reinforcement learning,the model achieves adaptive multilevel feature fusion between modalities,supporting learningmore effective alignment strategies.Extensive experiments on multiple public datasets show that the MPSEA method achieves gains of up to 7% in Hits@1 and 8.2% in MRR on the FBDB15K dataset,and up to 9.1% in Hits@1 and 7.7% in MRR on the FBYG15K dataset,compared to existing state-of-the-art methods.These results confirm the effectiveness of the proposed model.
基金supported by the National Key Research and Development Program of China(2017YFC1600802)Henan Provincial Science and Technology Research Project,China(No.232102110134)the National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang of China(No.FE202303).
文摘In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undatus (Haw.) Britton & Rose (H. undatus) has not been reported yet. Through transcriptomic analysis, virus-induced gene silencing (VIGS) technology, and RT-qPCR validation, we investigated the role of the LBD TFs in the senescence of H. undatus. Transcriptomic analysis revealed that HuLBD1 is a key transcription factor of the LBD family regulating H. undatus senescence. After silencing HuLBD1, 5075 differentially expressed genes (DEGs) were identified. GSEA results showed that these DEGs were mainly enriched in the phenylpropanoids and flavonoid biosynthesis pathway. The leading subset of genes in these two pathways included 16 DEGs, with four upregulated genes, HuCHS1-2, HuFLS1, HuCYP75B2, and HuCHS5-2 being central in the Protein-Protein Interaction (PPI) network. H. undatus phenotypic experiments confirmed that silencing HuLBD1 significantly increased the flavonoid content in the fruit peel, and RT-qPCR validation showed that the gene expression trends were consistent with the RNA-seq data. The study indicated that HuLBD1 likely delays the fruit senescence process by negatively regulating the expression of four key genes, including HuCHS1-2, thus promoting the synthesis of phenylpropanoids such as flavonoids. In conclusion, our findings suggest that the HuLBD1 TF plays an important role in the senescence of H. undatus fruit, providing a theoretical basis for postharvest senescence control.
基金supported by the National Natural Science Foundation of China(No.21471162)the Hunan Provincial Science and Technology Plan Project(No.2017TP1001)Postgraduate Innovation project(No.502211822)。
文摘The development of high-performance layered oxide cathodes for sodium ion batteries (SIBs) continues to facing be hindered by severe challenges to date.Herein,a single-phase P2-Na0.67Mn0.6Ni0.2Co0.1Cu0.1O2(NMNCC) comprising multiple-layer-oriented stacked nanoflakes is designed and synthesized via a simple sol-gel method.The large lattice parameters ensure a large three-dimensional frame,which enables the diffusion of sodium ions.Owing to its optimal morphology structure modulation transition metal substitution strategy,the MNCC electrode delivers a reversible capacity of 131.3 mAh g^-1 at 0.1 C with retention of 86.7%after 200 cycles.In addition,it provides an initial capacity of 86.7 mAh g^-1,and a retention of 80.0%after 500 cycles even at a current density of up to 1 A g^-1.The stable single-phase structure and slight volume shrinkage observed after Na+extraction further delay structural degradation.High Na+mobility and low Na+diffusion resistance are also guarantee the excellent rate performance of the NMNCC electrode.Thus,we determine that the NMNCC cathode is significant in the advancement of promising novel layered oxide cathodes.
基金supported by the National Natural Science Foundation of China(81970265,82270310)a Sub-Project of the National Key R&D Program“The recognition and Identification of Genetic Pathogenic Genes for Structural Birth Defects”(2021YFC2701002)+1 种基金Nanjing Science and Technology Development Project(2019060007)Jiangsu Provincial Key Research and Development Program(BE2023662).
文摘The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the results of multi-center clinical nutrition research for congenital heart disease following thefirst Chinese consensus edition of 2016.The consensus was also shaped by the results of three discussion sessions and two questionnaires con-ducted by the 13-member collaboration group.This process was informed by both clinical guidelines and expert consensus.The quality of literature,both in English and Chinese,and the level of recommendations were evaluated using the Grading of Recommendations Assessment,Development,and Evaluations(GRADE)system.
基金financially supported by the National Supporting Program(2013BAD12B05)Natural Science Foundation of China(NSFC)(no.31101839 and no.31302117)+1 种基金the key program of Gansu Province(no.1013JHTA008)Jiangsu Co-innovation Center program for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
文摘African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus(r NDV) expressing ASFV protein 72(p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, r NDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in r NDV/p72 was stably maintained through ten passages. Mice immunized with r NDV/p72 developed high titers of ASFV p72 specific Ig G antibody, and had higher levels of Ig G1 than IgG2 a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that r NDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.
基金supported by the National Natural Science Foundation Council of China(82172386 and 81922081 to C.L.,82100943 to X.F.,82104216 to J.L.,and 82230081,82250710175 and 8226116039 to G.X.)the Department of Education of Guangdong Province(2021KTSCX104 to C.L.)+5 种基金the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund(Guangdong-Hong Kong-Macao Joint Lab)(2020B1212030006 to A.L.)the Guangdong Provincial Science and Technology Innovation Council Grant(2017B030301018 to G.X.)the Guangdong Basic and Applied Basic Research Foundation(2022A1515012164 to C.L.,and 2023A1515012000 to X.F.)the Science,Technology and Innovation Commission of Shenzhen(JCYJ20210324104201005 to C.L.,JCYJ20220530115006014 to X.F.,JCYJ20230807095118035 to J.L.,and JCYJ20220818100617036 to G.X.)the Hong Kong General Research Fund(12102722 to A.L.)the Hong Kong RGC Themebased Research Scheme(T12-201/20-R to A.L.).
文摘Rheumatoid arthritis(RA)is an autoimmune disease.Early studies hold an opinion that gut microbiota is environmentally acquired and associated with RA susceptibility.However,accumulating evidence demonstrates that genetics also shape the gut microbiota.It is known that some strains of inbred laboratory mice are highly susceptible to collagen-induced arthritis(CIA),while the others are resistant to CIA.Here,we show that transplantation of fecal microbiota of CIA-resistant C57BL/6J mice to CIA-susceptible DBA/1J mice confer CIA resistance in DBA/1J mice.C57BL/6J mice and healthy human individuals have enriched B.fragilis than DBA/1J mice and RA patients.Transplantation of B.fragilis prevents CIA in DBA/1J mice.We identify that B.fragilis mainly produces propionate and C57BL/6J mice and healthy human individuals have higher level of propionate.Fibroblast-like synoviocytes(FLSs)in RA are activated to undergo tumor-like transformation.Propionate disrupts HDAC3-FOXK1 interaction to increase acetylation of FOXK1,resulting in reduced FOXK1 stability,blocked interferon signaling and deactivation of RA-FLSs.We treat CIA mice with propionate and show that propionate attenuates CIA.Moreover,a combination of propionate with anti-TNF etanercept synergistically relieves CIA.These results suggest that B.fragilis or propionate could be an alternative or complementary approach to the current therapies.
基金supported by the National Natural Science Foundation of China(Grants No.42371354,42375129)Fundamental Research Funds for National University,China University of Geosciences,Wuhan(Grant No.CUGDCJJ202201).
文摘Climate change threatens China’s rice production,making it crucial to assess the impact of climate change and climate year type(CYT)on rice production across regions to safeguard food security.The impact of climate change under nine CYTs with different combinations of temperature and precipitation on two rice cropping systems,including single rice and double rice(early and late rice)was evaluated.The results indicate that:(1)the Northeast region was expected to undergo the greatest warming of 2.03–2.48℃,and future climate conditions would be dominated by Warm-Humid,Warm-Normal,and Warm-Dry CYTs across all regions.(2)Climate change would significantly shorten anthesis days after sowing and maturity days after sowing of single rice by 6–12 days and 9–24 days,with little change observed for late rice(<1 day).Late rice yield suffered more from climate change compared to single and early rice yield,declining by 8.8%–16.13%.(3)Different CYTs exhibited varying impacts on rice yields.Yields were projected to decrease by approximately 4.765%to 18.645%in Warm-Humid,Warm-Normal,and Warm-Dry CYTs.Conversely,the Northeast region was anticipated to experience an increase in yield.(4)Relationships between rice yield and meteorological factors varied by region,variety,and CYT.Among the nine CYTs,high killing degree days,mean daily temperature,mean solar radiation and warm spell duration index were the main factors influencing changes in rice yield,explaining nearly 80%of yield change.Our results would help to develop adaptation strategies in different regions and rice cropping systems.
文摘Neurosyphilis(NS)is an infectious disease caused by Treponema pallidum invading the central nervous system.It can manifest at any stage of syphilis,and is often misdiagnosed due to its atypical and progressive symptoms.The increasing incidence of NS underscores the necessity for early and accurate diagnosis.Here,we present a case where routine cerebrospinal fluid metagenomic next-generation sequencing(mNGS)was used to diagnose a patient with neurosyphilis.The patient exhibited cognitive impairment and was initially diagnosed with cerebral infarction due to syphilitic cerebral arteritis.Thus,the patient was treated with dual antiplatelet therapy(aspirin and clopidogrel)and statins to stabilize the plaques.Neurosyphilis was treated with penicillin sodium injections,resulting in significant improvement in the patient’s mental state.This case is a rare instance of neurosyphilis associated with cerebral infarction.These findings suggest that mNGS is a valuable tool in diagnosing neurosyphilis,potentially improving diagnostic accuracy and patient outcomes.
基金supported by the National Key Research and Development Program of China(2017YFC1600802)Henan Provincial Science and Technology Research Project,China(No.232102110134).
文摘Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites.Flavonoids are natural compounds that possess many important biological activities,playing a significant role in the medicinal activity of Eucommia ulmoides(E.ulmoides).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in E.ulmoides,a comprehensive analysis of metabolomics and transcriptomics was conducted.The results indicated that salt stress led to the wilting and darkening of E.ulmoides leaves,accompanied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical conductivity.During salt stress,most metabolites in the flavonoid biosynthesis pathway of E.ulmoides were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt stress.Among them,secondary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt stress.The correlation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as 6-Hydroxyluteolin.The co-expression network showed a strong positive correlation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative correlation with Catechin biosynthesis.The branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid compound biosynthesis in the plant stress response during salt stress.The results of this study provided a preliminary mechanism of secondary metabolites such as flavonoids in the medicinal plant E.ulmoides induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress response.It also provided useful information for subsequent exploration of resistance genes in E.ulmoides.
基金This work was supported by the National Key Research and Development Program of China(2017YFC1600802)the National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang of China(No.FE202303).
文摘During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseflavonoid compound levels and reduce oxidative damage.To investigate the mechanism of trypsin-inducedflavonoid bio-synthesis in C.sativus,we conducted a combined analysis of transcriptomics and widely targeted metabolomics.One hundred and seventy-five significantly different metabolites were obtained from metabolomics data.The kyoto encyclopedia of genes and genomes(KEGG)functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway.By combining the results of the weighted gene co-expression network analysis(WGCNA)with the 130 upregulated phenylpropanoid metabolites,22 significantly upregulated phenylpropanoid metabolites were identified.Trilobatin was identified as the most prominent meta-bolite through cluster analysis and variable importance in projection(VIP)analysis.High performance liquid chro-matography(HPLC)experiments confirmed that trilobatin was the key metabolite induced by trypsin.The transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated withflavonoid biosynthesis.The gene set enrichment analysis(GSEA)identified leading edges in 4 key KEGG path-ways.Finally,combined with WGCNA and GSEA analysis results,35 core genes were obtained.The co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of trilobatin.The quantitative real-time polymerase chain reaction(RT-qPCR)and dual luciferase experiments con-firmed the activation effect of CsWRKY28 on CsMYC2 and downstream target genes.This study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in C.sativus and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.
基金China’s National Natural Science Foundation provided funding for this study(81900222)Guangzhou Science and Technology Program(SL2022A04J01269,202201020646)Guangzhou Health Science and Technology Program(20211A010026).
文摘Aims:Multiple genes and environmental factors are known to be involved in congenital heart disease(CHD),but epigenetic variation has received little attention.Monozygotic(MZ)twins with CHD provide a unique model for exploring this phenomenon.In order to investigate the potential role of Deoxyribonucleic Acid(DNA)methyla-tion in CHD pathogenesis,the present study examined DNA methylation variation in MZ twins discordant for CHD,especially ventricular septal defect(VSD).Methods and Results:Using genome-wide DNA methylation profiles,we identified 4004 differentially methylated regions(DMRs)in 18 MZ twin pairs discordant for CHD,and 2826 genes were identified.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed a list of CHD-associated pathways.To further investigate the role of DNA methylation in VSD,data from 7 pairs of MZ twins with VSD were analyzed.We identified 1614 DMRs corresponding to 1443 genes associated with arrhythmogenic right ventricular cardiomyopathy,cyclic guanosine monopho-sphate-protein kinase G(cGMP-PKG)signaling pathway by KEGG analysis,and cell-cell adhesion,calcium ion transmembrane transport by GO analysis.A proportion of DMR-associated genes were involved in calcium signaling pathways.The methylation changes of calcium signaling genes might be related to VSD pathogenesis.Conclusion:CHD is associated with differential DNA methylation in MZ twins.CHD may be etiologically linked to DNA methylation,and methylation of calcium signaling genes may be involved in the development of VSD.
基金supported partly by the Health Talent Project of Jilin Province(JLSWSRCZX2021 to Xiang Wang,China)the General Project of Natural Science Foundation of Jilin Province(YDZJ202201ZYTS013 to Xiang Wang,China)the National Natural Science Foundation Youth Program(82200397 to Xiang Wang,China).
文摘Objective:Limited data are available regarding current outcomes after revascularization for angiographically confirmed very late stent thrombosis(VLST).Therefore,this study investigated the current outcomes of patients with VLST and the risk factors for major adverse cardiovascular events(MACE).Methods:Consecutive patients who underwent revascularization for angiographically confirmed VLST between January 2014 and January 2016 were enrolled in this study.The patients were divided into two groups according to the occurrence of MACE during follow-up.The clinical and interventional parameters between groups were compared.Results:A total of 564 patients with an average age of 61.61±10.71 years were enrolled.Eight patients died during hospitalization.MACE occurred in 100 patients after a mean follow-up of 620 days.The total MACE rate was 35.46%,and Kaplan-Meier survival analysis indicated an estimated MACE-free survival rate of 22%.Multivariable Cox regression analysis revealed that left ventricular ejection fraction and peak troponin I at VLST onset were independent predictors of MACE.Conclusions:Long-term outcomes after revascularization for VLST remained unfavorable.Left ventricular ejection fraction and peak troponin I at the onset of VLST were independent risk factors for poor prognosis.
基金The National Natural Science Foundation of China,Grant/Award Numbers:12271118,12331017。
文摘Gene transcription is a stochastic process characterized by fluctuations in mRNA levels of the same gene in isogenic cell populations.A central question in single-cell studies is how to map transcriptional variability to phenotypic differences between isogenic cells.We introduced a measurable and statistical transcription threshold I for critical genes that determine the entry level of Waddington’s canal toward a specific cell fate.Subsequently,J_(I),which is the probability that a cell has at least I mRNA molecules of a given gene,approximates the likelihood of a cell committing to the corresponding fate.In this study,we extended the previous results of J_(I) of the classical telegraph model by considering more complex models with different gene activation frameworks.We showed that(a)the upregulation of the critical gene may significantly suppress cell fate change and(b)increasing transcription noise performs a bidirectional role that can either enhance or suppress the cell fate change.These observations matched accurately with the data from bacterial,yeast,and mammalian cells.We estimated the threshold I from these data and predicted that(a)the traditional human immunodeficiency virus(HIV)activators that modulate gene activation frequency at high doses may largely suppress HIV reactivation and(b)the cells may favor noisier(or less noisy)regulation of stress genes under high(or low)environmental pressures to maintain cell viability.
基金supported in part by American Diabetes Association(1-18-IBS-082 to LC,USA)the National Key R&D Program of China(2016YFC0900903 to YZ,China)。
文摘Herein,we define the role of ferroptosis in the pathogenesis of diabetic cardiomyopathy(DCM)by examining the expression of key regulators of ferroptosis in mice with DCM and a new ex vivo DCM model.Advanced glycation end-products(AGEs),an important pathogenic factor of DCM,were found to induce ferroptosis in engineered cardiac tissues(ECTs),as reflected through increased levels of Ptgs2 and lipid peroxides and decreased ferritin and SLC7 A11 levels.Typical morphological changes of ferroptosis in cardiomyocytes were observed using transmission electron microscopy.Inhibition of ferroptosis with ferrostatin-1 and deferoxamine prevented AGE-induced ECT remodeling and dysfunction.Ferroptosis was also evidenced in the heart of type 2 diabetic mice with DCM.Inhibition of ferroptosis by liproxstatin-1 prevented the development of diastolic dysfunction at 3 months after the onset of diabetes.Nuclear factor erythroid 2-related factor 2(NRF2)activated by sulforaphane inhibited cardiac cell ferroptosis in both AGE-treated ECTs and hearts of DCM mice by upregulating ferritin and SLC7 A11 levels.The protective effect of sulforaphane on ferroptosis was AMP-activated protein kinase(AMPK)-dependent.These findings suggest that ferroptosis plays an essential role in the pathogenesis of DCM;sulforaphane prevents ferroptosis and associated pathogenesis via AMPK-mediated NRF2 activation.This suggests a feasible therapeutic approach with sulforaphane to clinically prevent ferroptosis and DCM.
基金the financial assistance of the National Natural Science Foundation of China(Grant No.71110107028,71033003,71273237 and 70803047)the grant from Poverty and Economic Policy Research Network(PEP)The generous supports of local officials and especially those from Ningshan County Government
文摘A significant gap remains between rural and urban students in the rate of admission to senior high school. One reason for this gap might be the high levels of tuition and fees for senior high school By reducing students＇ expectations of attending high school, high levels of tuition and fees could be reducing student aeademic performance in junior high schools. In this paper we evaluate the impacts of a senior high tuition relief program on the test scores of poor rural seventh grade students in China. We surveyed three counties in Shaanxi Province and exploited the fact that, while the counties are adjacent to one another and share similar characteristics, only one of the three implemented a tuition relief program. Using several alternative estimation strategies, including difference-in-differences, propensity score matching and difference-in-differences matching, we find that the tuition program has a statistically significant and positive impact on the mathematics scores of seventh grade students. More importantly, this program is shown to have a statistically significant and positive effect on the poorest students in the treatment group compared to their wealthier peers.
