Neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),amyotrophic lateral sclerosis(ALS),and huntington’s disease,pose significant threats to human health,with current treatment op-tion...Neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),amyotrophic lateral sclerosis(ALS),and huntington’s disease,pose significant threats to human health,with current treatment op-tions remaining limited.Piezoelectric materials,known for their ability to convert mechanical energy into electrical signals at the nanoscale,hold great promise in the diagnosis and treatment of neurodegenerative diseases due to their excellent electromechanical properties,environmental stability,and sensitivity.This review systematically outlines the working principles and classifications of piezoelectric materials.Subsequently,the recent advances in piezoelectric materials and their applications in the diagnosis and treatment of neurode-generative diseases are highlighted.Finally,the challenges and perspectives regarding the development of future piezoelectric materials are discussed.This review aims to provide a comprehensive reference for the further application of piezoelectric materials in neurodegenerative diseases.展开更多
Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessiv...Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessive fibrotic behavior.Glycolysis dysregulation has been shown to participate in the incidence and progression of various fibrotic diseases and shows potential as a means of controlling HS formation.This work aimed to discuss the impact of augmented glycolysis on HS and to propose a method for controlling HS formation through glycolysis regulation.Methods:Here,augmented glycolysis was confirmed together with enhanced fibrotic activity in both HS fibroblasts(HFs)and HS tissues,and the suppression of glycolysis also attenuated fibroblast activation.We also introduced IR780,a heptamethine cyanine dye,to regulate glycolysis for the control of HS formation.Results:In vitro,cell studies indicated that IR780 significantly down-regulated glycolysis and suppressed the fibrotic activity of HFs.In vivo,the intralesional injection of IR780 into rabbit HS models led to the downregulation of glycolysis and the control of HS formation.Furthermore,IR780 accumulated preferentially in activated fibroblasts in both in vitro and in vivo studies,and thus specifically downregulated glycolysis and efficiently controlled fibrosis by targeting activated fibroblasts.Conclusions:This work identified a strategy for controlling fibrosis and HS formation from the perspective of glycolysis regulation with IR780 targeting of activated fibroblasts.展开更多
Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-amin...Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-aminolevulinic acid(ALA,a commercial photosensitizer in dermatology)result in an insufficient ROS generation,and thus limit the clinical application of PDT treating HS(HS-PDT).Consequently,sophisticated transdermal co-delivery nanoethosomes(named A/A-ES)with ALA and Au nanotriangles(AuNTs)in cores are prepared via an in-situ seed-mediated growth method,and then applied to improve HS-PDT through localized surface plasmon resonance(LSPR)-enhanced ROS generation.A/A-ES display a satisfactory performance in co-delivery in HS tissue with sufficient protoporphyrin IX production and LSPR effect in cytoplasm,which is beneficial for ALA excitation as well as ROS generation.In vitrolvivo studies reveal that A/A-ES significantly improve HS-PDT in promoting to fibroblast apoptosis and collagen remodeling through LSPR-enhanced ROS generation.Therefore,this study provides a feasible strategy that integrates transdermal delivery and LSPR to enable the beneficial effects of HS-PDT through boosting the delivery and excitation of ALA.展开更多
The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including...The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live imaging.The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse model.However,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee joints.In summary,NIR-II imaging could precisely identify the in vivo degradation rate of collagen.Moreover,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.展开更多
基金supported by the National Natural Science Foundation of China(82172222,82000456)Shanghai Jiao Tong University“Star Project”of Biomedical Multi-discipline Research Program(YG2024ZD13).
文摘Neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),amyotrophic lateral sclerosis(ALS),and huntington’s disease,pose significant threats to human health,with current treatment op-tions remaining limited.Piezoelectric materials,known for their ability to convert mechanical energy into electrical signals at the nanoscale,hold great promise in the diagnosis and treatment of neurodegenerative diseases due to their excellent electromechanical properties,environmental stability,and sensitivity.This review systematically outlines the working principles and classifications of piezoelectric materials.Subsequently,the recent advances in piezoelectric materials and their applications in the diagnosis and treatment of neurode-generative diseases are highlighted.Finally,the challenges and perspectives regarding the development of future piezoelectric materials are discussed.This review aims to provide a comprehensive reference for the further application of piezoelectric materials in neurodegenerative diseases.
基金supported by National Natural Science Foundation of China(82172222 and 82102328)ShanghaiMunicipal Education Commission-Gaofeng Clinical Medicine Grant Support(20152227)+3 种基金ClinicalMulti-Disciplinary Team Research Program of ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(2017-1-007)Cross Research Project of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202009)Shanghai Health Industry Clinical Research Special Project(20204Y0443)Shanghai Municipal Key Clinical Specialty(shslczdzk00901).
文摘Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessive fibrotic behavior.Glycolysis dysregulation has been shown to participate in the incidence and progression of various fibrotic diseases and shows potential as a means of controlling HS formation.This work aimed to discuss the impact of augmented glycolysis on HS and to propose a method for controlling HS formation through glycolysis regulation.Methods:Here,augmented glycolysis was confirmed together with enhanced fibrotic activity in both HS fibroblasts(HFs)and HS tissues,and the suppression of glycolysis also attenuated fibroblast activation.We also introduced IR780,a heptamethine cyanine dye,to regulate glycolysis for the control of HS formation.Results:In vitro,cell studies indicated that IR780 significantly down-regulated glycolysis and suppressed the fibrotic activity of HFs.In vivo,the intralesional injection of IR780 into rabbit HS models led to the downregulation of glycolysis and the control of HS formation.Furthermore,IR780 accumulated preferentially in activated fibroblasts in both in vitro and in vivo studies,and thus specifically downregulated glycolysis and efficiently controlled fibrosis by targeting activated fibroblasts.Conclusions:This work identified a strategy for controlling fibrosis and HS formation from the perspective of glycolysis regulation with IR780 targeting of activated fibroblasts.
基金This study was supported by China Postdoctoral Science Foundation(Nos.2017M620159 and 2019T120345)National Natural Science Foundation of China(Nos.81772098 and 81801917),Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support(No.20152227)+3 种基金Cross Research Project of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(No.JYJC202009)Shanghai Health Industry Clinical Research Special Project(No.20204Y0443)Shanghai Municipal Key Clinical Specialty(shslczdzk00901)Scientific Research Foundation of Shanghai Municipal Commission of Health and Family Planning(No.20154Y002).
文摘Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-aminolevulinic acid(ALA,a commercial photosensitizer in dermatology)result in an insufficient ROS generation,and thus limit the clinical application of PDT treating HS(HS-PDT).Consequently,sophisticated transdermal co-delivery nanoethosomes(named A/A-ES)with ALA and Au nanotriangles(AuNTs)in cores are prepared via an in-situ seed-mediated growth method,and then applied to improve HS-PDT through localized surface plasmon resonance(LSPR)-enhanced ROS generation.A/A-ES display a satisfactory performance in co-delivery in HS tissue with sufficient protoporphyrin IX production and LSPR effect in cytoplasm,which is beneficial for ALA excitation as well as ROS generation.In vitrolvivo studies reveal that A/A-ES significantly improve HS-PDT in promoting to fibroblast apoptosis and collagen remodeling through LSPR-enhanced ROS generation.Therefore,this study provides a feasible strategy that integrates transdermal delivery and LSPR to enable the beneficial effects of HS-PDT through boosting the delivery and excitation of ALA.
基金supported by National Key R&D Program of China(2021YFA1201303)National Natural Science Foundation of China(82172511,81972121,81972129,82072521,82011530023 and 82111530200)+5 种基金Sanming Project of Medicine in Shenzhen(SZSM201612078)the Introduction Project of Clinical Medicine Expert Team for Suzhou(SZYJTD201714)Shanghai Talent Development Funding Scheme(2020080)Shanghai Sailing Program(21YF1404100 and 22YF1405200)Shanghai Committee of Science and Technology(22DZ2204900)Medical Engineering Joint Fund of Fudan University(YG2022-14).
文摘The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live imaging.The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse model.However,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee joints.In summary,NIR-II imaging could precisely identify the in vivo degradation rate of collagen.Moreover,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.
