Objectives To investigate the change and clinical significance of clopidogrel on platelet membrane CD40L in coronary artery disease patients before and after percutaneous coronary intervention(PCI).Methods 30 cases wh...Objectives To investigate the change and clinical significance of clopidogrel on platelet membrane CD40L in coronary artery disease patients before and after percutaneous coronary intervention(PCI).Methods 30 cases who were diagnosis coronary artery diseases(CAD)by coronary angiography,mean age 56±9 years old.All the patients who had no antiplatelet aggregation contraindication,were treated with standard anti angina pectoris drugs.Before PCI,all the patients took clopidogrel 75 mg per day.Activated platelet membrane CD40L express rate was measured by flow cytometry before and after PCI 6 hours.Results Activated platelet membrane CD40L express rate were 3.73±2.15 and 2.46±0.90,respectively in 30 patients before and after PCI 6 hours.Activated platelet membrane CD40L express rate was significantly decrease after PCI 6 hours than that before PCI(P<0.01).Conclusions Clopidogrel has significance effect on platelet membrane CD40L in coronary artery disease patients undergoing PCI.Clopidogrel can suppression platelet activation and prevent thromboembolism event occurrence.展开更多
Designing efficient electrocatalysts for the hydrogen evolution reaction （HER） has attracted substantial attention owing to the urgent demand for clean energy to face the energy crisis and subsequent environmental i...Designing efficient electrocatalysts for the hydrogen evolution reaction （HER） has attracted substantial attention owing to the urgent demand for clean energy to face the energy crisis and subsequent environmental issues in the near future. Among the large variety of HER catalysts, molybdenum disulfide （MoS2） has been regarded as the most famous catalyst owing to its abundance, low price, high efficiency, and definite catalytic mechanism. In this study, defect-engineered MoS2 nanowall （NW） catalysts with controllable thickness were fabricated and exhibited a significantly enhanced HER performance. Benefiting from the highly exposed active edge sites and the rough surface accompanied by the robust NW structure, the defect-rich MoS2 NW catalyst with an optimized thickness showed an ultralow onset overpotential of 85 mV, a high current density of 310.6 mA·cm^-2 at η = 300 mV, and a low potential of 95 mV to drive a 10 mA·cm^-2 cathodic current. Additionally, excellent electrochemical stability was realized, making this freestanding NW catalyst a promising candidate for practical water splitting and hydrogen production.展开更多
Protein fibrillation is recognized as an attractive strategy to broaden and improve the function of food proteins.This research investigated the influence of different NaCl concentrations(0-210 mM)on the thermal aggre...Protein fibrillation is recognized as an attractive strategy to broaden and improve the function of food proteins.This research investigated the influence of different NaCl concentrations(0-210 mM)on the thermal aggregation behavior of rice proteins(RP)at pH 2.0.Under lower(<100 mM)and higher(>100 mM)NaCl concentrations,RP formed aggregates with different sizes and structures.At lower concentrations,the zeta-potential of RP increased from 14.3 mV to 22.8 mV during incubation,indicating that the electrostatic repulsion of RP was enhanced and numerous positively charged building blocks were generated.The circular dichroism(CD)results indicated that the native structure of RP was destroyed after heating at 90℃ for 24 h,and a secondary structure dominated byβ-sheets(more than 50%)was formed.Proteins formed long fibrils after 24 h heating,as observed by atomic force microscope(AFM).However,at higher NaCl concentrations,the hydrophobic interaction was the dominant force due to electrostatic shielding.The protein aggregated randomly to form amorphous aggregates with particle sizes over 600 nm.This study will provide a new insight of RP-based fibrils in food application.展开更多
Cadmium(Cd)is a toxic heavy metal and leads to various altered physiological functions,which are harmful to health.This study aimed to evaluate the protective effect of insoluble dietary fiber from rice bran(RBIDF,non...Cadmium(Cd)is a toxic heavy metal and leads to various altered physiological functions,which are harmful to health.This study aimed to evaluate the protective effect of insoluble dietary fiber from rice bran(RBIDF,non-starch polysaccharides)against Cd-induced toxicity in mice.The results showed that Cd exposure caused a significant decrease in body weight and that RBIDF recovered the body weight gain of mice(P<0.05).Moreover,RBIDF reversed the decrease of liver and kidney weight,and the increase of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(CREA)and urea(UREA)levels.The histopathological results showed that RBIDF alleviated severe liver damage induced by Cd exposure,and improved liver cell injury and glomerulus destruction.RBIDF also decreased the Cd accumulation in the intestine and Cd absorption and increased Cd content in mouse feces(P<0.05).In addition,at phylum level,RBIDF increased Bacteroidetes abundance,while decreased Firmicutes and Proteobacteria abundances.At genus level,it increased Lactobacillus abundance as well.It could be seen that RBIDF ameliorated the Cd-induced decrease in gut microbiota disorders.The results indicated that RBIDF was an effective component in the daily diet against Cd toxicity.展开更多
文摘Objectives To investigate the change and clinical significance of clopidogrel on platelet membrane CD40L in coronary artery disease patients before and after percutaneous coronary intervention(PCI).Methods 30 cases who were diagnosis coronary artery diseases(CAD)by coronary angiography,mean age 56±9 years old.All the patients who had no antiplatelet aggregation contraindication,were treated with standard anti angina pectoris drugs.Before PCI,all the patients took clopidogrel 75 mg per day.Activated platelet membrane CD40L express rate was measured by flow cytometry before and after PCI 6 hours.Results Activated platelet membrane CD40L express rate were 3.73±2.15 and 2.46±0.90,respectively in 30 patients before and after PCI 6 hours.Activated platelet membrane CD40L express rate was significantly decrease after PCI 6 hours than that before PCI(P<0.01).Conclusions Clopidogrel has significance effect on platelet membrane CD40L in coronary artery disease patients undergoing PCI.Clopidogrel can suppression platelet activation and prevent thromboembolism event occurrence.
基金Acknowledgements This work was financially supported by the National Basic Research Program of China （No. 2015CB932302）, the National Natural Science Foundation of China （Nos. 21501112, 21331005, 21401181, U1532265, U1632149, 91422303, and 11321503）, and Natural Science Foundation of Shandong Province （No. ZR2014BQ007）.
文摘Designing efficient electrocatalysts for the hydrogen evolution reaction （HER） has attracted substantial attention owing to the urgent demand for clean energy to face the energy crisis and subsequent environmental issues in the near future. Among the large variety of HER catalysts, molybdenum disulfide （MoS2） has been regarded as the most famous catalyst owing to its abundance, low price, high efficiency, and definite catalytic mechanism. In this study, defect-engineered MoS2 nanowall （NW） catalysts with controllable thickness were fabricated and exhibited a significantly enhanced HER performance. Benefiting from the highly exposed active edge sites and the rough surface accompanied by the robust NW structure, the defect-rich MoS2 NW catalyst with an optimized thickness showed an ultralow onset overpotential of 85 mV, a high current density of 310.6 mA·cm^-2 at η = 300 mV, and a low potential of 95 mV to drive a 10 mA·cm^-2 cathodic current. Additionally, excellent electrochemical stability was realized, making this freestanding NW catalyst a promising candidate for practical water splitting and hydrogen production.
基金support for this research was provided by the National Natural Science Foundation of China(No.32072263)Jiangsu rice green production and intensive processing technology integration innovation and demonstration application(No.BE2019395)+2 种基金the Key R&D Program of Jiangsu Province(BE202113592-2)the Fundamental Research Funds for the Central Universities(JUSRP622010)Agricultural Research Outstanding Talents Training Program(13210291).
文摘Protein fibrillation is recognized as an attractive strategy to broaden and improve the function of food proteins.This research investigated the influence of different NaCl concentrations(0-210 mM)on the thermal aggregation behavior of rice proteins(RP)at pH 2.0.Under lower(<100 mM)and higher(>100 mM)NaCl concentrations,RP formed aggregates with different sizes and structures.At lower concentrations,the zeta-potential of RP increased from 14.3 mV to 22.8 mV during incubation,indicating that the electrostatic repulsion of RP was enhanced and numerous positively charged building blocks were generated.The circular dichroism(CD)results indicated that the native structure of RP was destroyed after heating at 90℃ for 24 h,and a secondary structure dominated byβ-sheets(more than 50%)was formed.Proteins formed long fibrils after 24 h heating,as observed by atomic force microscope(AFM).However,at higher NaCl concentrations,the hydrophobic interaction was the dominant force due to electrostatic shielding.The protein aggregated randomly to form amorphous aggregates with particle sizes over 600 nm.This study will provide a new insight of RP-based fibrils in food application.
基金support for this research was provided by National Natural Science Foundation of China(No.32072263)National Natural Science Foundation of China(No.32001737)+3 种基金Key R&D Program of Jiangsu Province(BE202113592-2)Agricultural Research Outstanding Talents Training Program(13210291)the Fundamental Research Funds for the Central Universities(JUSRP122007)Qinghai Province Science and Technology Department Project(2019-SF-C15).
文摘Cadmium(Cd)is a toxic heavy metal and leads to various altered physiological functions,which are harmful to health.This study aimed to evaluate the protective effect of insoluble dietary fiber from rice bran(RBIDF,non-starch polysaccharides)against Cd-induced toxicity in mice.The results showed that Cd exposure caused a significant decrease in body weight and that RBIDF recovered the body weight gain of mice(P<0.05).Moreover,RBIDF reversed the decrease of liver and kidney weight,and the increase of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(CREA)and urea(UREA)levels.The histopathological results showed that RBIDF alleviated severe liver damage induced by Cd exposure,and improved liver cell injury and glomerulus destruction.RBIDF also decreased the Cd accumulation in the intestine and Cd absorption and increased Cd content in mouse feces(P<0.05).In addition,at phylum level,RBIDF increased Bacteroidetes abundance,while decreased Firmicutes and Proteobacteria abundances.At genus level,it increased Lactobacillus abundance as well.It could be seen that RBIDF ameliorated the Cd-induced decrease in gut microbiota disorders.The results indicated that RBIDF was an effective component in the daily diet against Cd toxicity.
