[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods...[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods]The chromatographic separation was performed on a GL Sciences(19H0044724)-C_(18)column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.05%formic acid solution at a flow rate of 1 mL/min.The injection volume was 10μL,detection wavelength was set at 280 nm,and column temperature was maintained at 25℃.[Results]The linear ranges of forsythin,baicalin,and chlorogenic acid were 10.5-52.5,20.6-103,and 14.2-71μg/mL,respectively,with good linear relationships between concentration and peak area(R^(2)=0.9999).The relative standard deviations(RSD s)for precision and repeatability tests were all≤1.0%.The average recoveries were 98.51%,98.48%,and 97.71%for the three components,with RSD s of 0.96%,0.97%,and 0.73%,respectively.[Conclusions]This method demonstrates strong specificity,high precision,excellent accuracy,and simplicity of operation,making it suitable for the simultaneous quantification of forsythin,baicalin,and chlorogenic acid in traditional Chinese medicine spray disinfectants.It provides a reliable basis for quality control and practical applications in animal breeding environments.展开更多
基金Supported by Shandong Province Major Agricultural Technologies Collaborative Promotion Project(SDNYXTTG-2024-04)National Key Laboratory for Quality Control of Chinese Medicinal Materials and Decoction Pieces Project(2024GSMPA-KL16)Weifang Municipal Science and Technology Bureau Project(2021GX031).
文摘[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods]The chromatographic separation was performed on a GL Sciences(19H0044724)-C_(18)column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.05%formic acid solution at a flow rate of 1 mL/min.The injection volume was 10μL,detection wavelength was set at 280 nm,and column temperature was maintained at 25℃.[Results]The linear ranges of forsythin,baicalin,and chlorogenic acid were 10.5-52.5,20.6-103,and 14.2-71μg/mL,respectively,with good linear relationships between concentration and peak area(R^(2)=0.9999).The relative standard deviations(RSD s)for precision and repeatability tests were all≤1.0%.The average recoveries were 98.51%,98.48%,and 97.71%for the three components,with RSD s of 0.96%,0.97%,and 0.73%,respectively.[Conclusions]This method demonstrates strong specificity,high precision,excellent accuracy,and simplicity of operation,making it suitable for the simultaneous quantification of forsythin,baicalin,and chlorogenic acid in traditional Chinese medicine spray disinfectants.It provides a reliable basis for quality control and practical applications in animal breeding environments.
