Plant synthetic biology has emerged as a transformative field in agriculture,offering innovative solutions to enhance food security,provide resilience to climate change,and transition to sustainable farming practices....Plant synthetic biology has emerged as a transformative field in agriculture,offering innovative solutions to enhance food security,provide resilience to climate change,and transition to sustainable farming practices.By integrating advanced genetic tools,computational modeling,and systems biology,researchers can precisely modify plant genomes to enhance traits such as yield,stress tolerance,and nutrient use efficiency.The ability to design plants with specific characteristics tailored to diverse environmental conditions and agricultural needs holds great potential to address global food security challenges.Here,we highlight recent advancements and applications of plant synthetic biology in agriculture,focusing on key areas such as photosynthetic efficiency,nitrogen fixation,drought tolerance,pathogen resistance,nutrient use efficiency,biofortification,climate resilience,microbiology engineering,synthetic plant genomes,and the integration of artificial intelligence with synthetic biology.These innovations aim to maximize resource use efficiency,reduce reliance on external inputs,and mitigate environmental impacts associated with conventional agricultural practices.Despite challenges related to regulatory approval and public acceptance,the integration of synthetic biology in agriculture holds immense promise for creating more resilient and sustainable agricultural systems,contributing to global food security and environmental sustainability.Rigorous multi-field testing of these approaches will undoubtedly be required to ensure reproducibility.展开更多
Carbon assimilation is a crucial part of the photosynthetic process,wherein inorganic carbon,typically in the form of CO_(2),is converted into organic compounds by living organisms,including plants,algae,and a subset ...Carbon assimilation is a crucial part of the photosynthetic process,wherein inorganic carbon,typically in the form of CO_(2),is converted into organic compounds by living organisms,including plants,algae,and a subset of bacteria.Although several carbon fixation pathways have been elucidated,the Calvin-Benson-Bassham(CBB)cycle remains fundamental to carbon metabolism,playing a pivotal role in the biosynthesis of starch and sucrose in plants,algae,and cyanobacteria.However,Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO),the key carboxylase enzyme of the CBB cycle,exhibits low kinetic efficiency,low substrate specificity,and high temperature sensitivity,all of which have the potential to limit flux through this pathway.Consequently,RuBisCO needs to be present at very high concentrations,which is one of the factors contributing to its status as the most prevalent protein on Earth.Numerous attempts have been made to optimize the catalytic efficiency of RuBisCO and thereby promote plant growth.Furthermore,the limitations of this process highlight the potential benefits of engineering or discovering more efficient carbon fixation mechanisms,either by improving RuBisCO itself or by introducing alternative pathways.Here,we review advances in artificial carbon assimilation engineering,including the integration of synthetic biology,genetic engineering,metabolic pathway optimization,and artificial intelligence in order to create plants capable of performing more efficient photosynthesis.We additionally provide a perspective of current challenges and potential solutions alongside a personal opinion of the most promising future directions of this emerging field.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Category B,XDB1090000).
文摘Plant synthetic biology has emerged as a transformative field in agriculture,offering innovative solutions to enhance food security,provide resilience to climate change,and transition to sustainable farming practices.By integrating advanced genetic tools,computational modeling,and systems biology,researchers can precisely modify plant genomes to enhance traits such as yield,stress tolerance,and nutrient use efficiency.The ability to design plants with specific characteristics tailored to diverse environmental conditions and agricultural needs holds great potential to address global food security challenges.Here,we highlight recent advancements and applications of plant synthetic biology in agriculture,focusing on key areas such as photosynthetic efficiency,nitrogen fixation,drought tolerance,pathogen resistance,nutrient use efficiency,biofortification,climate resilience,microbiology engineering,synthetic plant genomes,and the integration of artificial intelligence with synthetic biology.These innovations aim to maximize resource use efficiency,reduce reliance on external inputs,and mitigate environmental impacts associated with conventional agricultural practices.Despite challenges related to regulatory approval and public acceptance,the integration of synthetic biology in agriculture holds immense promise for creating more resilient and sustainable agricultural systems,contributing to global food security and environmental sustainability.Rigorous multi-field testing of these approaches will undoubtedly be required to ensure reproducibility.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Category B,XDB1090000).
文摘Carbon assimilation is a crucial part of the photosynthetic process,wherein inorganic carbon,typically in the form of CO_(2),is converted into organic compounds by living organisms,including plants,algae,and a subset of bacteria.Although several carbon fixation pathways have been elucidated,the Calvin-Benson-Bassham(CBB)cycle remains fundamental to carbon metabolism,playing a pivotal role in the biosynthesis of starch and sucrose in plants,algae,and cyanobacteria.However,Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO),the key carboxylase enzyme of the CBB cycle,exhibits low kinetic efficiency,low substrate specificity,and high temperature sensitivity,all of which have the potential to limit flux through this pathway.Consequently,RuBisCO needs to be present at very high concentrations,which is one of the factors contributing to its status as the most prevalent protein on Earth.Numerous attempts have been made to optimize the catalytic efficiency of RuBisCO and thereby promote plant growth.Furthermore,the limitations of this process highlight the potential benefits of engineering or discovering more efficient carbon fixation mechanisms,either by improving RuBisCO itself or by introducing alternative pathways.Here,we review advances in artificial carbon assimilation engineering,including the integration of synthetic biology,genetic engineering,metabolic pathway optimization,and artificial intelligence in order to create plants capable of performing more efficient photosynthesis.We additionally provide a perspective of current challenges and potential solutions alongside a personal opinion of the most promising future directions of this emerging field.
