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高效体积排阻色谱法定量检测口蹄疫疫苗中146S的疫苗预处理方法 被引量:4
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作者 宋艳民 杨延丽 +6 位作者 苏志国 刘丽丽 朱元源 徐嫄 邹兴启 赵启祖 张松平 《生物工程学报》 CAS CSCD 北大核心 2019年第8期1441-1452,共12页
旨在建立一种口蹄疫灭活病毒疫苗的处理方法,去除尺寸排阻色谱法(HPSEC)检测146S抗原过程中的杂质干扰,获得最佳的检测信号并实现146S抗原含量的准确测定。分别考察了超速离心法、PEG沉淀法、核酸酶消化法对两批疫苗样品中的HPSEC检测... 旨在建立一种口蹄疫灭活病毒疫苗的处理方法,去除尺寸排阻色谱法(HPSEC)检测146S抗原过程中的杂质干扰,获得最佳的检测信号并实现146S抗原含量的准确测定。分别考察了超速离心法、PEG沉淀法、核酸酶消化法对两批疫苗样品中的HPSEC检测干扰杂质的去除效果。在优化条件下,超速离心法处理后146S检测结果分别为7.1、7.6 μg/mL,PEG沉淀法为9.7、10.4 μg/mL;酶消化法处理后的检测值最高,分别为10.5、10.4 μg/mL,且杂质去除完全、处理速度快、操作条件温和。通过响应面法确定最优酶消化处理条件如下:200 μL水相中添加终浓度421 U/mL的Benzonase,25.1 ℃下反应1.29 h。在该最优条件下,对4家企业各3种不同血清型共12批疫苗样品进行146S含量检测,结果表明建立的方法对不同疫苗均有良好适用性,且重复性好(RSD<5.3%,n=3)。通过该处理方法,解决了杂质对146S定量检测的干扰,扩大了HPSEC检测技术的应用范围,进一步确保了检测结果的准确可靠。 展开更多
关键词 尺寸排阻色谱 口蹄疫疫苗 146S 定量 酶消化
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Measuring the impact of human–AI collaboration on knowledge diffusion in new product development projects
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作者 Ying HAN Qing YANG +3 位作者 xingqi zou Pingye TIAN Yang FENG Tao YAO 《Frontiers of Engineering Management》 2025年第4期899-915,共17页
Artificial Intelligence (AI) is playing an increasingly pivotal role in New Product Development (NPD) project management.We propose a comprehensive framework to explore the impact of human–AI collaboration on organiz... Artificial Intelligence (AI) is playing an increasingly pivotal role in New Product Development (NPD) project management.We propose a comprehensive framework to explore the impact of human–AI collaboration on organizational knowledge diffusion.First,we develop a knowledge diffusion model based on continuous human–AI interactions,and we use the Agent-Based Modeling (ABM) method to simulate the diffusion process within the collaborative team and assess diffusion rates and efficiency based on knowledge levels.Second,we examine the interdependencies among members under different roles of AI,integrating AI cognitive capabilities,human–AI cognitive trust,and task interdependencies,and build a tie strength measurement model from the Social Network Analysis (SNA) perspective.Third,an entropy-based model is introduced to measure AI’s cognitive capability,accounting for project complexity and AI-generated solution uncertainty.We also establish a dynamic cognitive trust model that incorporates both the dynamic nature of trust in human–AI interactions and AI’s cognitive capability.Task interdependencies are assessed through a multi-dimensional activity network,and visualized by the Dependency Structure Matrix (DSM) method.Finally,an industrial example is provided to demonstrate the proposed model.Results show that organizational knowledge diffusion performs best when AI acts both as a collaborator and a tool.Moreover,this paper provides new insights,including how trust and task interdependencies significantly impact knowledge diffusion in human–AI collaborative organizations. 展开更多
关键词 human–AI collaboration knowledge diffusion TRUST Agent-Based Modeling(ABM) product development project
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