Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on t...Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.展开更多
Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose me...Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose metabolism.By limiting invertase activity,INHs keep cellular sugar levels elevated,which provides enhanced protection to plants under stress.Our results showed that the expression of PpVIN2,the only vacuolar invertase(VIN)gene in peach fruit sensitive to chilling temperatures,increases significantly during cold storage,while VIN enzyme activity increases more modestly.We also found that peach fruit transiently overexpressing PpINH1 had decreased VIN activity.Interactions of PpINH1 and PpVIN2 with recombinant proteins were shown by yeast two-hybrid assays and bimolecular fluorescence complementation assays,as well as in vitro.During cold storage,trehalose-treated peach fruit had significantly increased PpINH1 expression,decreased VIN enzyme activity,and significantly higher sucrose content than did untreated fruit.As a result,the treated fruit had enhanced resistance to chilling injury.Collectively,our data show that the post-translational repression of VIN enzyme activity by PpINH1 helps maintain sucrose levels in peach fruit during cold storage,thereby improving resistance to chilling injury.展开更多
To study the changes in sugar metabolism caused by fungal infection in post-harvest peaches,fruit from two cultivars(‘Baifeng’and‘Yulu’)was inoculated with Monilinia fructicola and stored at 10°C.During disea...To study the changes in sugar metabolism caused by fungal infection in post-harvest peaches,fruit from two cultivars(‘Baifeng’and‘Yulu’)was inoculated with Monilinia fructicola and stored at 10°C.During disease development,soluble sugar content was monitored,as well as the activities and expression of selected enzymes.Disease progression was accompanied by a decrease in sucrose content and increases in reducing sugars and soluble solids,consistent with higher enzyme activities for acid invertase,neutral invertase and sucrose synthase-cleavage,and lower activities for sucrose synthase-synthesis and sucrose phosphate synthase.Activities of phosphofructokinase,hexokinase,and pyruvate kinase,which are related to hexose metabolism,also increased.These changes stimulate the Embden–Meyerhof–Parnas(EMP)pathway.We conclude that the fungal disease in peach fruit accelerates the decomposition of sucrose,thereby providing more glucose as a substrate to the EMP pathway.展开更多
A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are ofte...A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.展开更多
Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal ag...Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.展开更多
基金supported by the Zhejiang Key R&D Program of China(2020C02037)the Ningbo Agricultural Research Program of China(2022S1542022S138).
文摘Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.
基金supported by the National Key R&D Program of China(No.2018YFD1000200)National Science Foundation of China(Nos.31671903 and 31972124).
文摘Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose metabolism.By limiting invertase activity,INHs keep cellular sugar levels elevated,which provides enhanced protection to plants under stress.Our results showed that the expression of PpVIN2,the only vacuolar invertase(VIN)gene in peach fruit sensitive to chilling temperatures,increases significantly during cold storage,while VIN enzyme activity increases more modestly.We also found that peach fruit transiently overexpressing PpINH1 had decreased VIN activity.Interactions of PpINH1 and PpVIN2 with recombinant proteins were shown by yeast two-hybrid assays and bimolecular fluorescence complementation assays,as well as in vitro.During cold storage,trehalose-treated peach fruit had significantly increased PpINH1 expression,decreased VIN enzyme activity,and significantly higher sucrose content than did untreated fruit.As a result,the treated fruit had enhanced resistance to chilling injury.Collectively,our data show that the post-translational repression of VIN enzyme activity by PpINH1 helps maintain sucrose levels in peach fruit during cold storage,thereby improving resistance to chilling injury.
基金This study was sponsored by the National Science Foundation of China(No.31671903)the Nature Science Foundation of Zhejiang Province(No.LR15C200002)the K.C.Wong Magna Fund in Ningbo University.
文摘To study the changes in sugar metabolism caused by fungal infection in post-harvest peaches,fruit from two cultivars(‘Baifeng’and‘Yulu’)was inoculated with Monilinia fructicola and stored at 10°C.During disease development,soluble sugar content was monitored,as well as the activities and expression of selected enzymes.Disease progression was accompanied by a decrease in sucrose content and increases in reducing sugars and soluble solids,consistent with higher enzyme activities for acid invertase,neutral invertase and sucrose synthase-cleavage,and lower activities for sucrose synthase-synthesis and sucrose phosphate synthase.Activities of phosphofructokinase,hexokinase,and pyruvate kinase,which are related to hexose metabolism,also increased.These changes stimulate the Embden–Meyerhof–Parnas(EMP)pathway.We conclude that the fungal disease in peach fruit accelerates the decomposition of sucrose,thereby providing more glucose as a substrate to the EMP pathway.
基金supported by Natural Science Foundation of Zhejiang Province[LY16C200003]Zhejiang Provincial Key Research and Development,Program[2020C02037].
文摘A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.
基金This research was kindly supported through funding from National Natural Science Foundation of China(31772030)Student Research and Innovation Program of Ningbo University(2021SRIP3607).
文摘Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.
