Objective: To investigate the specific mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) in the regulation of human sperm apoptosis, and to provide a new theoretical reference and scientific basis for the diagno...Objective: To investigate the specific mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) in the regulation of human sperm apoptosis, and to provide a new theoretical reference and scientific basis for the diagnosis and treatment of asthenospermia and other related conditions. Methods: Semen samples were categorized into the normal group and asthenospermia group based on sperm motility criteria. HIF-1α interfering agent cobalt chloride (CoCl2) and guanylate cyclase activator (Lificiguat, YC-1) were added respectively, with a control group established accordingly. Sperm motility (using anterior viability rate as an index), apoptosis level, ATP level, mitochondrial membrane potential, and reactive oxygen species (ROS) level were measured. The expression levels of HIF-1α, p-PI3K, and Bcl-2 in the samples were analyzed using Western blotting. Results: Following CoCl2 treatment, there was a significant increase in sperm apoptosis compared to the normal control group (12.51% ± 2.50% VS 11.15% ± 2.42%);additionally, sperm motility (45.34% ± 3.37% VS 51.36% ± 11.68%), ATP production (11.51 ± 2.87 nM/µL VS 14.99 ± 2.83 nM/µL), ROS levels, and mitochondrial membrane potential all decreased significantly (all P α and p-PI3K increased significantly while Bcl-2 expression decreased (all P α in the YC-1 treatment group were decreased, and the expression level of Bcl-2 was increased (all P α can influence human sperm apoptosis and motility through the PI3K signaling pathway.展开更多
The research progress on the development and application of new natural tobacco humectants was reviewed in the paper.Plants,animals and microbes contain special moisturizing substances,showing distinctive advantages a...The research progress on the development and application of new natural tobacco humectants was reviewed in the paper.Plants,animals and microbes contain special moisturizing substances,showing distinctive advantages and outstanding effects in respect of the tobacco moisture content maintaining and cigarette suction comfort promoting.The development necessity,major types,functional mechanism and influence on cigarette quality of new natural tobacco humectants development were compared and elaborated.Additionally,the prospect of new natural tobacco humectants development and application was forecasted.展开更多
In recent years,bamboo has been widely used in a broad range of applications,a thorough understanding of the structural characteristics of bamboo nodes is essential for better processing and manufacturing of biomimeti...In recent years,bamboo has been widely used in a broad range of applications,a thorough understanding of the structural characteristics of bamboo nodes is essential for better processing and manufacturing of biomimetic materials.This study investigated the complex anatomical structure for the nodes of two bamboo species,Indocalamus latifolius(Keng)McClure and Shibataea chinensis Nakai,using a high-resolution X-ray microtomography(μCT).The results show that the vascular bundle system in the nodal region of I.latifolius and S.chinensis is a net-like structure composed of horizontal and axial vascular bundles.Furthermore,the fiber sheath surrounding metaxylem vessels tended to be shorter in the tangential direction.This structure of bamboo nodes facilitates the tangential and axial transport of moisture and nutrients.The anatomical structure of I.latifolius and S.chinensis nodes has obvious differences,especially in the arrangement of vascular bundles.Vascular bundle frequency was significantly higher in S.chinensis nodes than in I.latifolius nodes.These findings indicate thatμCT is a nondestructive three-dimensional imaging method that can used to examine the anatomical structure of bamboo nodes.展开更多
Chalcopyrite,copper indium gallium selenide(Cu(In,Ga)Se_(2),CIGS),as semiconductor materials,have been widely used as absorbers in thin-film solar cells,offering high power conversion efficiency(PCE)and good thermal s...Chalcopyrite,copper indium gallium selenide(Cu(In,Ga)Se_(2),CIGS),as semiconductor materials,have been widely used as absorbers in thin-film solar cells,offering high power conversion efficiency(PCE)and good thermal stability[1−3].Recently,the development of non-traditional photovoltaic(PV)devices such as semitransparent.展开更多
文摘Objective: To investigate the specific mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) in the regulation of human sperm apoptosis, and to provide a new theoretical reference and scientific basis for the diagnosis and treatment of asthenospermia and other related conditions. Methods: Semen samples were categorized into the normal group and asthenospermia group based on sperm motility criteria. HIF-1α interfering agent cobalt chloride (CoCl2) and guanylate cyclase activator (Lificiguat, YC-1) were added respectively, with a control group established accordingly. Sperm motility (using anterior viability rate as an index), apoptosis level, ATP level, mitochondrial membrane potential, and reactive oxygen species (ROS) level were measured. The expression levels of HIF-1α, p-PI3K, and Bcl-2 in the samples were analyzed using Western blotting. Results: Following CoCl2 treatment, there was a significant increase in sperm apoptosis compared to the normal control group (12.51% ± 2.50% VS 11.15% ± 2.42%);additionally, sperm motility (45.34% ± 3.37% VS 51.36% ± 11.68%), ATP production (11.51 ± 2.87 nM/µL VS 14.99 ± 2.83 nM/µL), ROS levels, and mitochondrial membrane potential all decreased significantly (all P α and p-PI3K increased significantly while Bcl-2 expression decreased (all P α in the YC-1 treatment group were decreased, and the expression level of Bcl-2 was increased (all P α can influence human sperm apoptosis and motility through the PI3K signaling pathway.
基金Supported by the Fund for the Open Project of Anhui Key Laboratory of Tobacco Chemistry (No.W2015JSKF0493 and W2015JSKF0492)the China Postdoctoral Science Foundation (No.2015T80646 and 2013M531501)the National Natural Science Foundation of China (No.31400049)
文摘The research progress on the development and application of new natural tobacco humectants was reviewed in the paper.Plants,animals and microbes contain special moisturizing substances,showing distinctive advantages and outstanding effects in respect of the tobacco moisture content maintaining and cigarette suction comfort promoting.The development necessity,major types,functional mechanism and influence on cigarette quality of new natural tobacco humectants development were compared and elaborated.Additionally,the prospect of new natural tobacco humectants development and application was forecasted.
基金This research was funded by the Nature Science Foundation of China(Grant No.31670565)the National Key Research&Development Program(No.2016YFD0600904).
文摘In recent years,bamboo has been widely used in a broad range of applications,a thorough understanding of the structural characteristics of bamboo nodes is essential for better processing and manufacturing of biomimetic materials.This study investigated the complex anatomical structure for the nodes of two bamboo species,Indocalamus latifolius(Keng)McClure and Shibataea chinensis Nakai,using a high-resolution X-ray microtomography(μCT).The results show that the vascular bundle system in the nodal region of I.latifolius and S.chinensis is a net-like structure composed of horizontal and axial vascular bundles.Furthermore,the fiber sheath surrounding metaxylem vessels tended to be shorter in the tangential direction.This structure of bamboo nodes facilitates the tangential and axial transport of moisture and nutrients.The anatomical structure of I.latifolius and S.chinensis nodes has obvious differences,especially in the arrangement of vascular bundles.Vascular bundle frequency was significantly higher in S.chinensis nodes than in I.latifolius nodes.These findings indicate thatμCT is a nondestructive three-dimensional imaging method that can used to examine the anatomical structure of bamboo nodes.
基金This work is supported by the National Natural Science Foundation of China(22075150)the National Key Research and Development Program of China(2019YFE0118100)+2 种基金L.Ding thanks the National Key Research and Development Program of China(2022YFB3803300)the open research fund of Songshan Lake Materials Laboratory(2021SLABFK02)the National Natural Science Foundation of China(21961160720).
文摘Chalcopyrite,copper indium gallium selenide(Cu(In,Ga)Se_(2),CIGS),as semiconductor materials,have been widely used as absorbers in thin-film solar cells,offering high power conversion efficiency(PCE)and good thermal stability[1−3].Recently,the development of non-traditional photovoltaic(PV)devices such as semitransparent.
