Portal vein thrombosis(PVT)is a common complication during liver transplantation,with an incidence of approximately 5-26%among patients with end-stage liver disease(1).Its presence of PVT significantly increases surgi...Portal vein thrombosis(PVT)is a common complication during liver transplantation,with an incidence of approximately 5-26%among patients with end-stage liver disease(1).Its presence of PVT significantly increases surgical complexity,potentially leading to failure of portal vein reconstruction,postoperative anastomotic stenosis,or even graft liver failure(1,2).展开更多
Black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is a promi-nent insect for the bioconversion of various organic wastes.As a saprotrophic insect,the BSF inhabits microbe-rich environments.However,the in...Black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is a promi-nent insect for the bioconversion of various organic wastes.As a saprotrophic insect,the BSF inhabits microbe-rich environments.However,the influences of the intestinal mi-croorganisms on BSF growth and development are not very clear.In this study,the dy-namics of the intestinal bacterial community of BSF larvae(BSFL)were analyzed using pyrosequencing.Actinobacteria,Bacteroidetes,Firmicutes,and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars.The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level.Klebsiella,Clostridium,Providencia,and Dysgonomonas were the relatively most abundant bacteria in the 1st-to 4th-instar BSFL,respectively.Dysgonomonas and Providencia also dominated the 5th-and 6th-instar larvae,at ratios of 31.1%and 47.2%,respectively.In total,148 bacterial strains affiliated with 20 genera were isolated on differ-ent media under aerobic and anaerobic conditions.Among them,6 bacteria,BSF1-BSF6,were selected for further study.The inoculation of the 6 isolates independently into germ-free BSFL feeding on an artificial diet showed that all the bacteria,except BSF4,sig-nificantly promoted BSF growth and development compared with the germ-free control.Citrobacter,Dysgonomonas,Klebsiella,Ochrobactrum,and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae,as well as increasing the prepupae and eclosion rates.In addition,Citrobacter,Klebsiella and Prov-idencia shortened the BSF life cycle significantly.The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.展开更多
基金supported by grants from the National Natural Science Foundation of China(Nos.82470690 and 92159202)Hainan Province Science and Technology Special Fund(No.ZDYF2025LCLH012)+1 种基金the National Key R&D Program of China(No.2023YFC2306800)the Major Research Plan of Key Research and Development Project of Zhejiang Province(Nos.2024C03149 and 2023C03046).
文摘Portal vein thrombosis(PVT)is a common complication during liver transplantation,with an incidence of approximately 5-26%among patients with end-stage liver disease(1).Its presence of PVT significantly increases surgical complexity,potentially leading to failure of portal vein reconstruction,postoperative anastomotic stenosis,or even graft liver failure(1,2).
基金supported by the National Natural Science Foundation of China(31670004)the Fundamental Research Funds for the Central Universities,Central China Normal University(KJ02072019-0383 and CCNU20CG005).
文摘Black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is a promi-nent insect for the bioconversion of various organic wastes.As a saprotrophic insect,the BSF inhabits microbe-rich environments.However,the influences of the intestinal mi-croorganisms on BSF growth and development are not very clear.In this study,the dy-namics of the intestinal bacterial community of BSF larvae(BSFL)were analyzed using pyrosequencing.Actinobacteria,Bacteroidetes,Firmicutes,and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars.The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level.Klebsiella,Clostridium,Providencia,and Dysgonomonas were the relatively most abundant bacteria in the 1st-to 4th-instar BSFL,respectively.Dysgonomonas and Providencia also dominated the 5th-and 6th-instar larvae,at ratios of 31.1%and 47.2%,respectively.In total,148 bacterial strains affiliated with 20 genera were isolated on differ-ent media under aerobic and anaerobic conditions.Among them,6 bacteria,BSF1-BSF6,were selected for further study.The inoculation of the 6 isolates independently into germ-free BSFL feeding on an artificial diet showed that all the bacteria,except BSF4,sig-nificantly promoted BSF growth and development compared with the germ-free control.Citrobacter,Dysgonomonas,Klebsiella,Ochrobactrum,and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae,as well as increasing the prepupae and eclosion rates.In addition,Citrobacter,Klebsiella and Prov-idencia shortened the BSF life cycle significantly.The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
