为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲...为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。展开更多
[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with ...[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.展开更多
[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese o...[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese on day 28 with the same genetic background and similar initial weight(1 384.83.73±0.76 g) were randomly assigned into 3 groups, with 4 replicates per group and 10 geese per replicate. Geese were fed the diets containing 4.29%, 5.29% and 6.29 % crude fiber, respectively. The experiment lasted 35 d.[Result] Final weight and daily gain of 5.29 %CF group were significantly higher than those of 6.29 % CF group(P<0.05);Feed intake was markedly higher than those of 4.29% and 6.29 % CF groups(P<0.05);Feed gain ratio was significantly lower than that of 6.29 % CF group(P<0.05). Compared with 4.29% and 6.29 % CF groups, serum albumin concentrations of 5.29 % CF group increased significantly(P<0.05), while serum total protein and globin contents were higher(P>0.05), but serum uric acid level was lower(P>0.05).[Conclusion] 5.29 % crude fiber could lead to higher growth performance of Dingan geese.展开更多
In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat,six 7-month-old Hainan black goats and six hybrid offsprings were s...In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat,six 7-month-old Hainan black goats and six hybrid offsprings were selected to determine slaughter performance and muscle quality.The results showed that hybridization of Nubian black goat and Hainan black goat significantly improved the production performance and slaughter performance of hybrid offsprings(P>0.05).The meat quality of F1 hybrid black goat had no significant difference with that of Hainan black goat except for the hardness and elasticity(P<0.05).Therefore,the crossbred progenies of Nubian black goat and Hainan black goat have advantages in meat yield and meat performance,and the main meat quality indexes are not significantly different,which meets the market requirements for high-yield and high-quality hybrid combinations,and can be used for cross promotion.展开更多
[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of ...[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.展开更多
[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-d...[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-day-old Dingan geese with the same genetic background and similar initial weight(88.75±0.21g)were randomly assigned into 3 groups with 4 replicates per group and 12 geese per replicate,and fed with the diets containing 2.56%,3.52% and 4.48% crude fiber,respectively.The trial lasted 28 d.[Result]1)The final weight and daily gain of 3.52% CF group were significantly higher than those of 2.56% CF group(P<0.05);the feed/gain ratio was markedly lower than that of 2.56% CF group(P<0.05).2)The slaughter rate,semi-eviscerated rate and leg muscle rate of 3.52% CF group were higher than those of 2.56% and 4.48% CF groups(P>0.05).3)As the dietary crude fiber levels rose,the abdominal fat rate showed a downward trend(P>0.05).4)The liver index of 3.52% CF group was significantly higher than that of 2.56% CF group(P<0.05).4)Compared with 2.5%and 4.48% CF groups,the serum albumin concentrations of 3.52% CF group markedly increased(P<0.05),the serum total protein and globin contents were higher(P>0.05),but the serum uric acid level was lower(P>0.05).[Conclusion]When the crude fiber level was 3.52%,the production performances of Dingan geese aged from 1 to 28 days were better,and the liver index and serum albumin level could be obviously improved.展开更多
[Objective] This paper was to study effects of pelleted total mixed ration with different concentrate-roughage ratios on production performance and serum biochemical parameters of fattening Hainan black goats.[Method]...[Objective] This paper was to study effects of pelleted total mixed ration with different concentrate-roughage ratios on production performance and serum biochemical parameters of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic background and similar initial weight of(14.08±0.13) kg were randomly assigned into three treatments. Goats were fed with Juncao + concentrate(control group), pelleted total mixed ration I(concentrate: roughage 50 ∶50, group Ⅰ) and Ⅱ(concentrate: roughage 45∶55, group Ⅱ), respectively. The test lasted 45 d.[Result] The final weight, daily gain and feed intake of rams in experimental group Ⅰ were significantly higher than those of control group and experimental group Ⅱ(P<0.05);the final weight and daily gain of rams in experimental group Ⅰ were also markedly higher than those of experimental group Ⅱ(P<0.05);the feed/gain of rams in experimental group Ⅰ was significantly lower than those in control group and experimental group Ⅱ(P<0.05). The feed gain cost of rams in experimental group Ⅰ was markedly lower than that in experimental group Ⅱ( P<0.05), but was significantly higher than that in experimental group Ⅰ(P<0.05);the gross profit of rams in experimental group Ⅰ was significantly higher than those in control group and experimental group Ⅱ(P<0.05). Compared with control group and experimental group Ⅱ, the serum total protein and glucose lev-els in experimental group Ⅱ increased significantly(P<0.05), but the urea nitrogen content reduced(P>0.05).[Conclusion] When the concentrateroughage ratio of pelleted total mixed ration was 50∶50, the fattening effect of Hainan black goat was better, and the serum total protein and glucose levels were evidently improved.展开更多
[Objective] The paper was to understand the meat quality characteristics of Hainan black goat and its hybrid with Nubian black goat. [Method]Four 6-month-old Hainan black goats and Hainan black goat × Nubian blac...[Objective] The paper was to understand the meat quality characteristics of Hainan black goat and its hybrid with Nubian black goat. [Method]Four 6-month-old Hainan black goats and Hainan black goat × Nubian black goat F1 were slaughtered respectively,and the contents of crude protein,total fat,moisture,amino acids,mineral elements( Se,Zn and Mn) and fatty acid were determined. [Result]There was no significant difference in total fat,crude fat and moisture content in muscle of Hainan black goat × Nubian black goat F1 and Hainan black goat( P > 0. 05). The Fe content in the muscle of Hainan black goat × Nubian black goat F1 was significantly higher than that in Hainan black goat( P < 0. 05). There was no significant difference in EAA,NEAA,TAA and FAA between Hainan black goat × Nubian black goat F1 and Hainan black goat( P > 0. 05). The C17∶ 0 methyl heptadecanoate content in the muscle of Hainan black goat × Nubian black goat F1 was extremely lower than that in Hainan black goat( P < 0. 01),but the saturated fatty acid content and total unsaturated fatty acid content in the muscle of Hainan black goat × Nubian black goat F1 was lower than that in Hainan black goat( P > 0. 05). [Conclusion]The meat quality characteristics of Hainan black goat × Nubian black goat F1 had little difference with its female parent,and the goal of breeding had been basically achieved.展开更多
[Objective]The paper was to investigate the effects of compound acidifier on growth performance and serum biochemical parameters of heat-stressed Wenchang chickens.[Method]A total of 28881-day-old Wenchang chickens wi...[Objective]The paper was to investigate the effects of compound acidifier on growth performance and serum biochemical parameters of heat-stressed Wenchang chickens.[Method]A total of 28881-day-old Wenchang chickens with the same genetic background and similar initial weight(985.63±20.35 g)were randomly divided into four groups,four replicates per group and 18 chickens per replicate.Birds were fed the basal diet supplemented with 0 g/kg(control group),1 g/kg(groupⅠ),2 g/kg(groupⅡ)and 3 g/kg(groupⅢ)compound acidifier.The trial lasted 35 d.[Result]The daily gain of experimental groupⅠwas significantly higher than those of control group and groupⅢ(P<0.05),and the feed intake of experimental groupⅠwas also markedly higher than that of control group(P<0.05),but the feed gain ratio of experimental groupⅠwas significantly lower than that of groupⅢ(P<0.05).Compared with control group,the serum total protein and albumin levels of experimental groupⅠincreased(P>0.05),but the serum uric acid concentration decreased(P>0.05).[Conclusion]Supplementing 1 g/kg compound acidifier could improve the growth performance of heat-stressed Wenchang chickens.展开更多
文摘为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)
文摘[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.
基金Special Project for Technical Development of Hainan Scientific Research Institutions(SQ2017JSKF0008)China Agricultural Industry Research System(CARS-43-42)
文摘[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese on day 28 with the same genetic background and similar initial weight(1 384.83.73±0.76 g) were randomly assigned into 3 groups, with 4 replicates per group and 10 geese per replicate. Geese were fed the diets containing 4.29%, 5.29% and 6.29 % crude fiber, respectively. The experiment lasted 35 d.[Result] Final weight and daily gain of 5.29 %CF group were significantly higher than those of 6.29 % CF group(P<0.05);Feed intake was markedly higher than those of 4.29% and 6.29 % CF groups(P<0.05);Feed gain ratio was significantly lower than that of 6.29 % CF group(P<0.05). Compared with 4.29% and 6.29 % CF groups, serum albumin concentrations of 5.29 % CF group increased significantly(P<0.05), while serum total protein and globin contents were higher(P>0.05), but serum uric acid level was lower(P>0.05).[Conclusion] 5.29 % crude fiber could lead to higher growth performance of Dingan geese.
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01)Youth Science and Technology Talents Innovation Project of Hainan Association for Science and Technology(QCXM201802)
文摘In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat,six 7-month-old Hainan black goats and six hybrid offsprings were selected to determine slaughter performance and muscle quality.The results showed that hybridization of Nubian black goat and Hainan black goat significantly improved the production performance and slaughter performance of hybrid offsprings(P>0.05).The meat quality of F1 hybrid black goat had no significant difference with that of Hainan black goat except for the hardness and elasticity(P<0.05).Therefore,the crossbred progenies of Nubian black goat and Hainan black goat have advantages in meat yield and meat performance,and the main meat quality indexes are not significantly different,which meets the market requirements for high-yield and high-quality hybrid combinations,and can be used for cross promotion.
基金Supported by Technical Development Project of Provincial Scientific Research Institutes in Hainan(KYYS-2016-12)
文摘[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.
基金Supported by Special Fund for Technology Development of Hainan Scientific Research Institute(KYYS-2019-04)Natural Science Foundation of Hainan Province(318MS109)+1 种基金Special Fund for Construction of China Agricultural Industry Research System(CARS-43-42)Special Funds for Local Science and Technology Development Guided by the Central Committee(ZY2019HN01)
文摘[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-day-old Dingan geese with the same genetic background and similar initial weight(88.75±0.21g)were randomly assigned into 3 groups with 4 replicates per group and 12 geese per replicate,and fed with the diets containing 2.56%,3.52% and 4.48% crude fiber,respectively.The trial lasted 28 d.[Result]1)The final weight and daily gain of 3.52% CF group were significantly higher than those of 2.56% CF group(P<0.05);the feed/gain ratio was markedly lower than that of 2.56% CF group(P<0.05).2)The slaughter rate,semi-eviscerated rate and leg muscle rate of 3.52% CF group were higher than those of 2.56% and 4.48% CF groups(P>0.05).3)As the dietary crude fiber levels rose,the abdominal fat rate showed a downward trend(P>0.05).4)The liver index of 3.52% CF group was significantly higher than that of 2.56% CF group(P<0.05).4)Compared with 2.5%and 4.48% CF groups,the serum albumin concentrations of 3.52% CF group markedly increased(P<0.05),the serum total protein and globin contents were higher(P>0.05),but the serum uric acid level was lower(P>0.05).[Conclusion]When the crude fiber level was 3.52%,the production performances of Dingan geese aged from 1 to 28 days were better,and the liver index and serum albumin level could be obviously improved.
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)Youth Science and Technology Talents Innovation Project of Hainan Science&Technology Association(QCXM201802)
文摘[Objective] This paper was to study effects of pelleted total mixed ration with different concentrate-roughage ratios on production performance and serum biochemical parameters of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic background and similar initial weight of(14.08±0.13) kg were randomly assigned into three treatments. Goats were fed with Juncao + concentrate(control group), pelleted total mixed ration I(concentrate: roughage 50 ∶50, group Ⅰ) and Ⅱ(concentrate: roughage 45∶55, group Ⅱ), respectively. The test lasted 45 d.[Result] The final weight, daily gain and feed intake of rams in experimental group Ⅰ were significantly higher than those of control group and experimental group Ⅱ(P<0.05);the final weight and daily gain of rams in experimental group Ⅰ were also markedly higher than those of experimental group Ⅱ(P<0.05);the feed/gain of rams in experimental group Ⅰ was significantly lower than those in control group and experimental group Ⅱ(P<0.05). The feed gain cost of rams in experimental group Ⅰ was markedly lower than that in experimental group Ⅱ( P<0.05), but was significantly higher than that in experimental group Ⅰ(P<0.05);the gross profit of rams in experimental group Ⅰ was significantly higher than those in control group and experimental group Ⅱ(P<0.05). Compared with control group and experimental group Ⅱ, the serum total protein and glucose lev-els in experimental group Ⅱ increased significantly(P<0.05), but the urea nitrogen content reduced(P>0.05).[Conclusion] When the concentrateroughage ratio of pelleted total mixed ration was 50∶50, the fattening effect of Hainan black goat was better, and the serum total protein and glucose levels were evidently improved.
基金Supported by Applied Technology Research and Development and Demonstration Project of Hainan Province(ZDXM2015036)Key Science and Technology Project of Hainan Province(ZDKJ2016017)
文摘[Objective] The paper was to understand the meat quality characteristics of Hainan black goat and its hybrid with Nubian black goat. [Method]Four 6-month-old Hainan black goats and Hainan black goat × Nubian black goat F1 were slaughtered respectively,and the contents of crude protein,total fat,moisture,amino acids,mineral elements( Se,Zn and Mn) and fatty acid were determined. [Result]There was no significant difference in total fat,crude fat and moisture content in muscle of Hainan black goat × Nubian black goat F1 and Hainan black goat( P > 0. 05). The Fe content in the muscle of Hainan black goat × Nubian black goat F1 was significantly higher than that in Hainan black goat( P < 0. 05). There was no significant difference in EAA,NEAA,TAA and FAA between Hainan black goat × Nubian black goat F1 and Hainan black goat( P > 0. 05). The C17∶ 0 methyl heptadecanoate content in the muscle of Hainan black goat × Nubian black goat F1 was extremely lower than that in Hainan black goat( P < 0. 01),but the saturated fatty acid content and total unsaturated fatty acid content in the muscle of Hainan black goat × Nubian black goat F1 was lower than that in Hainan black goat( P > 0. 05). [Conclusion]The meat quality characteristics of Hainan black goat × Nubian black goat F1 had little difference with its female parent,and the goal of breeding had been basically achieved.
基金Supported by Special Fund for Technology Development of Hainan Provincial Research Institutes(SQ2017JSKF0007)Special Fund for Construction of China Agricultural Industry Research System(CARS-41-Z17)Special Funds for Local Scicncc and Tcchnoloty Development Guided by the Ccntoral Committee (ZY2019HN01)
文摘[Objective]The paper was to investigate the effects of compound acidifier on growth performance and serum biochemical parameters of heat-stressed Wenchang chickens.[Method]A total of 28881-day-old Wenchang chickens with the same genetic background and similar initial weight(985.63±20.35 g)were randomly divided into four groups,four replicates per group and 18 chickens per replicate.Birds were fed the basal diet supplemented with 0 g/kg(control group),1 g/kg(groupⅠ),2 g/kg(groupⅡ)and 3 g/kg(groupⅢ)compound acidifier.The trial lasted 35 d.[Result]The daily gain of experimental groupⅠwas significantly higher than those of control group and groupⅢ(P<0.05),and the feed intake of experimental groupⅠwas also markedly higher than that of control group(P<0.05),but the feed gain ratio of experimental groupⅠwas significantly lower than that of groupⅢ(P<0.05).Compared with control group,the serum total protein and albumin levels of experimental groupⅠincreased(P>0.05),but the serum uric acid concentration decreased(P>0.05).[Conclusion]Supplementing 1 g/kg compound acidifier could improve the growth performance of heat-stressed Wenchang chickens.
