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基于全基因组测序的大熊猫源厌氧大肠杆菌抗性与毒力基因分析
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作者 柳承希 邓雯文 +5 位作者 龙梅 杨盛智 韩新锋 李才武 赵珂 邹立扣 《遗传》 北大核心 2026年第2期213-226,I0001,共15页
大肠杆菌(Escherichia coli)是一种兼性厌氧菌,在大熊猫肠道中的检出率较高。本研究从13只大熊猫新鲜粪便中厌氧分离得到169株厌氧菌,其中13株大肠杆菌。通过全基因组测序及分析发现,大肠杆菌分为9个序列型(sequence type,ST),共检测到9... 大肠杆菌(Escherichia coli)是一种兼性厌氧菌,在大熊猫肠道中的检出率较高。本研究从13只大熊猫新鲜粪便中厌氧分离得到169株厌氧菌,其中13株大肠杆菌。通过全基因组测序及分析发现,大肠杆菌分为9个序列型(sequence type,ST),共检测到96种抗生素抗性基因、103种重金属抗性基因和213种毒力基因,其中mdtA、mdtB、mdtC和cusA、cusB、cusC、cusF基因在大肠杆菌中普遍存在。对其抗性与毒力基因的遗传环境进一步分析发现,mdtABC的上游存在baeS/baeR可能具有调控其表达产生抗性的能力;cusABCF上游携带铁载体相关的毒力基因fepA和entD、下游携带cusS/cusR;cusABCF上下游携带的可移动遗传元件有助于抗性基因、毒力基因的传播。本研究通过全基因组测序分析了大熊猫源厌氧大肠杆菌中存在的抗生素抗性基因、重金属抗性基因和毒力基因情况,对大熊猫的安全健康保护具有重要意义。 展开更多
关键词 大熊猫 大肠杆菌 厌氧 全基因组测序 抗性
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Bacillus and lactic acid bacteria inoculation to transform kitchen waste using Hermetia illucens
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作者 Guoyou Deng Min Yan +5 位作者 Shengzhi Yang Lin Ji xinfeng han Xiumei Yu Ke Zhao Likou Zou 《Journal of Environmental Sciences》 2026年第2期765-776,共12页
In this study,Bacillus mojavensis and Lactiplantibacillus herbarum were used to co-treat kitchen waste(KW)with Black soldier fly larvae(BSFL).The effects on the physicochemical properties,heavy metal content,and micro... In this study,Bacillus mojavensis and Lactiplantibacillus herbarum were used to co-treat kitchen waste(KW)with Black soldier fly larvae(BSFL).The effects on the physicochemical properties,heavy metal content,and microbial community of the BSFL sand were determined.Compared to the control group,the L.herbarum inoculation reduced 19.04%of the soluble salt(TSS),15.48%of Ni,and 13.04%of Zn in the residues;the B.mojavensis inoculation reduced 23.84%of TSS,13.61%of Pb,and 20.32%of the Ni in the residues;the L.herbarum and B.mojavensis inoculation reduced 29.53%of Cr,20.23%of Pb,18.06%of Ni,and 25.68%of the Zn in the residues.The microbial inoculants significantly enhanced the BSFL sand microbial diversity(Tukey,P<0.05).The dominant phylum and genus in the BSFL sand were Firmicutes(53.08%)and Corynebacterium(47.01%),respectively.The microbial inoculants resulted in an approximate 12%reduction in Corynebacterium.The linear discriminant analysis effective size analysis showed that the Corynebacterium abundance was significantly reduced.The microbial inoculants significantly affected the Corynebacterium relative abundance by significantly altering the substrate TSS,moisture content,and Ni.In conclusion,the effect of B.mojavensis and L.herbarum on the BSFL treatment of KW was beneficial,and their potential should be further exploited. 展开更多
关键词 Black soldier fly Bacteria Kitchen waste Waste management
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