A sulfate polysaccharide fraction(PYSP)was extracted and purified through DEAE cellucose-52 and Sephadex G-100 from Porphyra yezoensis.The PYSP is an acidic polysaccharide with a Mw of 152.93 kDa.PYSP was composed of ...A sulfate polysaccharide fraction(PYSP)was extracted and purified through DEAE cellucose-52 and Sephadex G-100 from Porphyra yezoensis.The PYSP is an acidic polysaccharide with a Mw of 152.93 kDa.PYSP was composed of galactose,glucose,and Glc-UA sugars with a molar ratio of 82.38:5.02:12.59.PYSP has an irregular fragmentation structure,with a sulfuric acid group content of 43.93%±0.47%.It suggested that PYSP has effective cholate and cholesterol adsorption capacity,α-glucosidase inhibition activity.When the concentration of PYSP was 1.0 mg/mL,the inhibition rates ofα-glucosidase were 45.02%.PYSP could decreaseα-helix and increaseβ-fold and random curl ofα-glucosidase.In addition,the in vitro hypoglycemic activities of PYSP were dosedependent.PYSP could improve glucose consumption,glycogen synthesis,and pyruvate kinase activity in insulin-resistant HepG2 cells.These results suggested that PYSP is a novel material for functional hypoglycemic foods.展开更多
基金supported by the Jiangsu Modern Agricultural Industry Technology System Construction Project(JATS[2022]167)the China Postdoctoral Science Foundation under Grant Number(2023M731402)the Jiangsu Funding Program for Excellent Post-doctoral Talent(2022ZB765).
文摘A sulfate polysaccharide fraction(PYSP)was extracted and purified through DEAE cellucose-52 and Sephadex G-100 from Porphyra yezoensis.The PYSP is an acidic polysaccharide with a Mw of 152.93 kDa.PYSP was composed of galactose,glucose,and Glc-UA sugars with a molar ratio of 82.38:5.02:12.59.PYSP has an irregular fragmentation structure,with a sulfuric acid group content of 43.93%±0.47%.It suggested that PYSP has effective cholate and cholesterol adsorption capacity,α-glucosidase inhibition activity.When the concentration of PYSP was 1.0 mg/mL,the inhibition rates ofα-glucosidase were 45.02%.PYSP could decreaseα-helix and increaseβ-fold and random curl ofα-glucosidase.In addition,the in vitro hypoglycemic activities of PYSP were dosedependent.PYSP could improve glucose consumption,glycogen synthesis,and pyruvate kinase activity in insulin-resistant HepG2 cells.These results suggested that PYSP is a novel material for functional hypoglycemic foods.
