Objective:To study the intervention effect of Peiyuan Huayu Decoction on the neuron damage in model rats with acute subdural hematoma (ASDH).Methods: 160 SD rats were randomly divided into four groups, and the ASDH mo...Objective:To study the intervention effect of Peiyuan Huayu Decoction on the neuron damage in model rats with acute subdural hematoma (ASDH).Methods: 160 SD rats were randomly divided into four groups, and the ASDH model rats were made by stereotactic autoblood injection, and sham operation group received craniotomy without blood injection. Sham operation group and model group were normally bred after model establishment, and 6 h after model establishment, the treatment group received intragastric administration of Peiyuan Huayu Decoction, and control group received intragastric administration of Piracetam Tablets, 1 time a day. On the 1d, 3d, 5d and 7d after model establishment, the general conditions of rats (activity, food intake and mental state) were observed, blood was collected via auricula dextra, ELISA method was used to determine peripheral plasma NSE and S100β protein contents, routine HE staining was conducted after perfusion fixation, the neurons in blood injection side of brain tissue were counted, and the neuron damage was observed.Results: 26 rats were dead in the experiment. The general conditions of sham operation group were significantly better than those of other groups, treatment group was significantly better than model group and control group on the 5d group (P<0.05), and there was no significant difference on the 1d, 3d and 7d (P>0.05);neuron count of sham operation group was basically stable, treatment group was not different from model group and control group on the 1d (P>0.05), treatment group was better than model group (P<0.05), and not different from control group (P>0.05) on the 3d, and treatment group was better than model group and control group on the 5d and 7d (P<0.05);peripheral plasma S100β protein and NSE contents of sham operation group were at lower levels, treatment group was not significantly different from model group and control group on the 1d (P>0.05), S100β protein and NSE contents decreased significantly on the 3d, and treatment group was significantly different from model group and control group (P<0.05), S100β protein and NSE contents increased on the 5d and 7d, the increase in treatment group was slower than that in model group and control group, and there was significant difference (P<0.05).Conclusion:Peiyuan Huayu Decoction has obvious protective effect on the neurons in ASDH model rats, and this effect may be based on the inhibition of secondary neuron damage.展开更多
Objective:The purpose of this study was to explore the effective compounds and molecular mechanism of Tong Xie Yao Fang(TXYF)in the treatment of Irritable Bowel Syndrome(IBS)based on network pharmacology.Method:The ef...Objective:The purpose of this study was to explore the effective compounds and molecular mechanism of Tong Xie Yao Fang(TXYF)in the treatment of Irritable Bowel Syndrome(IBS)based on network pharmacology.Method:The effective components in TXYF were screened using the TCMSP database,and the targets were predicted and analyzed with the PharmMapper server.The potential targets were imported into the MAS 3.0 database for target annotation and analysis.Finally,the KEGG database was used for pathway analysis.Result:Forty-three compounds were identified in the TXYF,corresponding to 247 targets.Among these,a total of 33 targets were found to be closely related to IBS.Pathway enrichment analysis identified 22 significant pathways to be associated with TXYF and its treatment.The results showed that components such as polyphenols,flavonoids,and terpenoids can enhance the immune function and alter hypoxia-induced stress by regulating cell proliferation,oxidative reactions,endocrine metabolism,and inflammatory reactions.Conclusion:The mechanism of TXYF in treating IBS is closely related to metabolic reactions,cell proliferation,inflammatory reactions,immune regulation,and endocrine hormone metabolism.展开更多
文摘Objective:To study the intervention effect of Peiyuan Huayu Decoction on the neuron damage in model rats with acute subdural hematoma (ASDH).Methods: 160 SD rats were randomly divided into four groups, and the ASDH model rats were made by stereotactic autoblood injection, and sham operation group received craniotomy without blood injection. Sham operation group and model group were normally bred after model establishment, and 6 h after model establishment, the treatment group received intragastric administration of Peiyuan Huayu Decoction, and control group received intragastric administration of Piracetam Tablets, 1 time a day. On the 1d, 3d, 5d and 7d after model establishment, the general conditions of rats (activity, food intake and mental state) were observed, blood was collected via auricula dextra, ELISA method was used to determine peripheral plasma NSE and S100β protein contents, routine HE staining was conducted after perfusion fixation, the neurons in blood injection side of brain tissue were counted, and the neuron damage was observed.Results: 26 rats were dead in the experiment. The general conditions of sham operation group were significantly better than those of other groups, treatment group was significantly better than model group and control group on the 5d group (P<0.05), and there was no significant difference on the 1d, 3d and 7d (P>0.05);neuron count of sham operation group was basically stable, treatment group was not different from model group and control group on the 1d (P>0.05), treatment group was better than model group (P<0.05), and not different from control group (P>0.05) on the 3d, and treatment group was better than model group and control group on the 5d and 7d (P<0.05);peripheral plasma S100β protein and NSE contents of sham operation group were at lower levels, treatment group was not significantly different from model group and control group on the 1d (P>0.05), S100β protein and NSE contents decreased significantly on the 3d, and treatment group was significantly different from model group and control group (P<0.05), S100β protein and NSE contents increased on the 5d and 7d, the increase in treatment group was slower than that in model group and control group, and there was significant difference (P<0.05).Conclusion:Peiyuan Huayu Decoction has obvious protective effect on the neurons in ASDH model rats, and this effect may be based on the inhibition of secondary neuron damage.
文摘Objective:The purpose of this study was to explore the effective compounds and molecular mechanism of Tong Xie Yao Fang(TXYF)in the treatment of Irritable Bowel Syndrome(IBS)based on network pharmacology.Method:The effective components in TXYF were screened using the TCMSP database,and the targets were predicted and analyzed with the PharmMapper server.The potential targets were imported into the MAS 3.0 database for target annotation and analysis.Finally,the KEGG database was used for pathway analysis.Result:Forty-three compounds were identified in the TXYF,corresponding to 247 targets.Among these,a total of 33 targets were found to be closely related to IBS.Pathway enrichment analysis identified 22 significant pathways to be associated with TXYF and its treatment.The results showed that components such as polyphenols,flavonoids,and terpenoids can enhance the immune function and alter hypoxia-induced stress by regulating cell proliferation,oxidative reactions,endocrine metabolism,and inflammatory reactions.Conclusion:The mechanism of TXYF in treating IBS is closely related to metabolic reactions,cell proliferation,inflammatory reactions,immune regulation,and endocrine hormone metabolism.
