Lunar wrinkle ridges are an important stress geological structure on the Moon, which reflect the stress state and geological activity on the Moon. They provide important insights into the evolution of the Moon and are...Lunar wrinkle ridges are an important stress geological structure on the Moon, which reflect the stress state and geological activity on the Moon. They provide important insights into the evolution of the Moon and are key factors influencing future lunar activity, such as the choice of landing sites. However, automatic extraction of lunar wrinkle ridges is a challenging task due to their complex morphology and ambiguous features. Traditional manual extraction methods are time-consuming and labor-intensive. To achieve automated and detailed detection of lunar wrinkle ridges, we have constructed a lunar wrinkle ridge data set, incorporating previously unused aspect data to provide edge information, and proposed a Dual-Branch Ridge Detection Network(DBR-Net) based on deep learning technology. This method employs a dual-branch architecture and an Attention Complementary Feature Fusion module to address the issue of insufficient lunar wrinkle ridge features. Through comparisons with the results of various deep learning approaches, it is demonstrated that the proposed method exhibits superior detection performance. Furthermore, the trained model was applied to lunar mare regions, generating a distribution map of lunar mare wrinkle ridges;a significant linear relationship between the length and area of the lunar wrinkle ridges was obtained through statistical analysis, and six previously unrecorded potential lunar wrinkle ridges were detected. The proposed method upgrades the automated extraction of lunar wrinkle ridges to a pixel-level precision and verifies the effectiveness of DBR-Net in lunar wrinkle ridge detection.展开更多
A ternary Ti35Zr28Nb alloy was fabricated by powder metallurgy(PM)from pre-alloyed powder.The microstructure,hardness,corrosion behavior,and wear response of the produced alloy were investigated systematically.The res...A ternary Ti35Zr28Nb alloy was fabricated by powder metallurgy(PM)from pre-alloyed powder.The microstructure,hardness,corrosion behavior,and wear response of the produced alloy were investigated systematically.The results show that nearly full dense Ti35Zr28Nb alloy(relative density is 98.1±1.2%)can be fabricated by PM.The microstructure was dominated with uniform phase.The Ti35Zr28Nb alloy displayed spontaneous passivity in a naturally aerated simulated body fluid(SBF)solution at 37±0.5°C.The Ti35Zr28Nb alloy exhibited the highest corrosion resistance as compared to as-cast Ti6Al4V and pure Ti because of the formation of a protective passive film containing TiO2,Nb2O5,and ZrO2,including the highest corrosion potential(-0.22±0.01 V),the lowest corrosion current density(57.45±1.88 nA),the lowest passive potential(0.05±0.01 V)and the widest passivation range(1.29±0.09 V).Under the same wear condition,the wear rate of the Ti35Zr28Nb alloy(0.0021±0.0002 mm3/m·N)was lower than that of the CP Ti(0.0029±0.0004 mm3/m·N)and close to that of the Ti6Al4V(0.0020±0.0003 mm3/m·N).The wear mechanism of the Ti35Zr28Nb alloy was mainly dominated by abrasive wear,accompanied by adhesive wear.The highest corrosion resistance together with the adequate wear resistance makes the PM-fabricated Ti35Zr28Nb alloy an attractive candidate for orthopedic implant materials.展开更多
BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review...BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review aims to explore the development of evaluation animal models of SAE,giving insight into the direction of future research in terms of its pathophysiology and therapy.METHODS:We performed a literature search from January 1,2000,to December 31,2022,in MEDLINE,PubMed,EMBASE,and Web of Science using related keywords.Two independent researchers screened all the accessible articles based on the inclusion and exclusion criteria and collected the relevant data of the studies.RESULTS:The animal models for sepsis are commonly induced through cecal ligation and puncture(CLP) or lipopolysaccharide(LPS) injection.SAE can be evaluated using nervous reflex scores and sepsis evaluation during the acute phase,or through Morris water maze(MWM),openfield test,fear condition(FC) test,inhibitory avoidance,and other tests during the late phase.CONCLUSION:CLP and LPS injection are the most common methods for establishing SAE animal models.Nervous reflexs cores,MWM,FC test,and inhibitory avoidance are widely used in SAE model analysis.Future research should focus on establishing a standardized system for SAE development and analysis.展开更多
BACKGROUND： Golgi protein 73 （GP73） is a promising bio- marker of hepatocellular carcinoma （HCC）. It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effec...BACKGROUND： Golgi protein 73 （GP73） is a promising bio- marker of hepatocellular carcinoma （HCC）. It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effectiveness of the treatment. But changes of GP73 after transcatheter arterial chemoemboliza- tion （TACE） have not been reported so far. This study was to investigate the dynamic changes of GP73 in HCC patients af- ter TACE treatment, and the possible underlying mechanisms in the cell cultures. METHODS： Blood samples were collected from 72 HCC pa- tients, before TACE, at day I and day 30 after TACE. GP73 lev- els were measured by Western blotting. The dynamic changes of GP73 were analyzed and compared with image changes and clinical data. The effects of chemotherapeutic agents （5-FU and pirarubicin） on GP73 expression were tested in three HCC cell lines （HepG2, HCCLM3 and MHCC97H）. RESULTS： The GP73 level was significantly elevated at day 1 and day 30 after TACE in HCC patients compared with that before the procedure （P〈0.05）. There was no statistical differ- ence between the two time points after TACE, nor correlationbetween GP73 levels and dinicopathological features, tumor metastasis, and patient survival. Pirarubicin, not 5-FU, signifi- cantly increased GP73 expression in three cell lines. CONCLUSIONS： Unlike surgical resection which decreases the GP73 level, TACE significantly increased GP73 expression in patients with HCC. No correlations were observed among GP73 levels, tumor characteristics and prognosis of patients with HCC.展开更多
BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism ...BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism for the relationship between GPR and poor cancer prognosis remains unclear,studies have demonstrated the clinical effect of both gamma-glutamyl transferase and platelet count on GBC and related gallbladder diseases.AIM To assess the prognostic value of GPR and to design a prognostic nomogram for GBC.METHODS The analysis involved 130 GBC patients who underwent surgery at Peking Union Medical College Hospital from December 2003 to April 2017.The patients were stratified into a high-or low-GPR group.The predictive ability of GPR was evaluated by Kaplan–Meier analysis and a Cox regression model.We developed a nomogram based on GPR,which we verified using calibration curves.The nomogram and other prognosis prediction models were compared using timedependent receiver operating characteristic curves and the concordance index.RESULTS Patients in the high-GPR group had a higher risk of jaundice,were older,and had higher carbohydrate antigen 19-9 levels and worse postoperative outcomes.Univariate analysis revealed that GPR,age,body mass index,tumor–node–metastasis(TNM)stage,jaundice,cancer cell differentiation degree,and carcinoembryonic antigen and carbohydrate antigen 19-9 levels were related to overall survival(OS).Multivariate analysis confirmed that GPR,body mass index,age,and TNM stage were independent predictors of poor OS.Calibration curves were highly consistent with actual observations.Comparisons of timedependent receiver operating characteristic curves and the concordance index showed advantages for the nomogram over TNM staging.CONCLUSION GPR is an independent predictor of GBC prognosis,and nomogram-integrated GPR is a promising predictive model for OS in GBC.展开更多
Algae-caused black bloom （also known as black water agglomerate） has recently become a critical problem in some Chinese lakes.It has been suggested that the occurrence of algae-caused black bloom was caused by the c...Algae-caused black bloom （also known as black water agglomerate） has recently become a critical problem in some Chinese lakes.It has been suggested that the occurrence of algae-caused black bloom was caused by the cooperation of nutrient-rich sediment with dead algae,and sludge dredging was adopted to control black bloom in some lakes of China.In this article,based on the simulation of black bloom using a Y-shape apparatus for modeling natural conditions,both un-dredged and dredged sites in three areas of Taihu Lake,China were studied to estimate the effects of dredging on the prevention and control of black bloom.During the experiment,drained algae were added to all six sites as an additional organic load;subsequently,the dissolved oxygen decreased rapidly,dropping to 0 mg/L at the sediment-water interface.Black bloom did not occur in the dredged sites of Moon Bay and Nan Quan,whereas all three un-dredged sites at Fudu Port,Moon Bay and Nan Quan experienced black bloom.Black bloom also occurred at the dredged site of Fudu Port one day later than at the other sites,and the odor and color were lighter than at the other locations.The color and odor of the black water mainly result from the presence of sulfides such as metal sulfides and hydrogen sulfide,among other chemicals,under reductive conditions.The color and odor of the water,together with the high concentrations of nutrients,were mainly caused by the decomposition of the algae and the presence of nutrient-rich sediment.Overall,the removal of the nutrient-rich sediment by dredging can prevent the occurrence and control the degree of algae-caused black bloom in Taihu Lake.展开更多
The challenge of producing a high-ductility titanium(Ti)material using inexpensive high-oxygen hydride-dehydride(HDH)Ti powder is hereby addressed by the incorporation of CaB_(6)oxygen-scavenger.The oxygen-scavenging ...The challenge of producing a high-ductility titanium(Ti)material using inexpensive high-oxygen hydride-dehydride(HDH)Ti powder is hereby addressed by the incorporation of CaB_(6)oxygen-scavenger.The oxygen-scavenging behavior,microstructure evolution,mechanical behavior and improvement mechanism were systematically investigated.A continuous TiO_(2)oxide layer with a thickness of approximately 9.3 nm is presented on the HDH Ti powder surface.The oxide layer will dissolve into Ti matrix during sintering,making the increase of c/a value and leading to the Ti-Ti bonds developing from plastic metal bonds to-ward brittle covalent bonds.The CaB_(6)addition can scavenge O impurity and make a significant increase in tensile ductility forα-Ti matrix.A small addition of 0.2 wt.%CaB_(6)provides a superior tensile elongation of 22.2%for Ti material,almost three times as high as that of pure Ti(7.5%).The increase of defor-mation twining activity and grain refinement are responsible for the improved ductility.Furthermore,the CaB_(6)oxygen-scavenger can react with the surface oxide layer to in-situ form rod-like TiB and granular CaTiO_(3)reinforcements,refining the coarse near equiaxed grain ofα-Ti matrix into fine equiaxed crys-tals.The multiple mechanisms of grain-boundary strengthening,load-bearing strengthening of TiB and Orowan strengthening of CaTiO_(3)nanoparticles work together to increase the tensile strength of Ti/CaB_(6)composites.This work offers an effective method to fabricate high-performance Ti material using inex-pensive high-oxygen HDH Ti powder.展开更多
Hot deformation of sintered billets by powder metallurgy(PM)is an effective preparation technique for titanium alloys,which is more significant for high-alloying alloys.In this study,Ti–6.5Al–2Zr–Mo–V(TA15)titaniu...Hot deformation of sintered billets by powder metallurgy(PM)is an effective preparation technique for titanium alloys,which is more significant for high-alloying alloys.In this study,Ti–6.5Al–2Zr–Mo–V(TA15)titanium alloy plates were prepared by cold press-ing sintering combined with high-temperature hot rolling.The microstructure and mechanical properties under different process paramet-ers were investigated.Optical microscope,electron backscatter diffraction,and others were applied to characterize the microstructure evolution and mechanical properties strengthening mechanism.The results showed that the chemical compositions were uniformly dif-fused without segregation during sintering,and the closing of the matrix craters was accelerated by increasing the sintering temperature.The block was hot rolled at 1200℃ with an 80%reduction under only two passes without annealing.The strength and elongation of the plate at 20–25℃ after solution and aging were 1247 MPa and 14.0%,respectively,which were increased by 24.5%and 40.0%,respect-ively,compared with the as-sintered alloy at 1300℃.The microstructure was significantly refined by continuous dynamic recrystalliza-tion,which was completed by the rotation and dislocation absorption of the substructure surrounded by low-angle grain boundaries.After hot rolling combined with heat treatment,the strength and plasticity of PM-TA15 were significantly improved,which resulted from the dense,uniform,and fine recrystallization structure and the synergistic effect of multiple slip systems.展开更多
Hepatocellular carcinoma is a common and fatal malignancy for which there is no effective systemic therapeutic strategy.Dihydroartemisinin(DHA),a derivative of artemisinin,has been shown to exert anti-tumor effects th...Hepatocellular carcinoma is a common and fatal malignancy for which there is no effective systemic therapeutic strategy.Dihydroartemisinin(DHA),a derivative of artemisinin,has been shown to exert anti-tumor effects through the production of reactive oxygen species(ROS)and resultant mitochondrial damage.However,clinical translation is limited by several drawbacks,such as insolubility,instability and low bioavailability.Here,based on a nanomedicine-based delivery strategy,we fabricated mitochondria-targeted carrier-free nanoparticles coupling DHA and triphenylphosphonium(TPP),aiming to improve bioavailability and mitochondrial targeting.DHA-TPP nanoparticles can be passively delivered to the tumor site by enhanced penetration and retention and then internalized.Flow cytometry and Western blot analysis showed that DHA-TPP nanoparticles increased intracellular ROS,which increased mitochondrial stress and in turn upregulated the downstream Bcl-2 pathway,leading to apoptosis.In vivo experiments showed that DHA-TPP nanoparticles exhibited anti-tumor effects in a mouse model of hepatocellular carcinoma.These findings suggest carrier-free DHA-TPP nanoparticles as a potential therapeutic strategy for hepatocellular carcinoma.展开更多
Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lowe...Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lower extremity deep venous thrombosis(LEDVT)in a severely traumatized population and to evaluate their predictive value for LEDVT.Methods This was a retrospective,single-center observational study.All subjects were severely traumatized patients who were admitted to the Traumatic Intensive Care Unit from January 2021 to May 2024.Based on Doppler ultrasound findings of both lower extremities from the time of injury to 30 days post-injury,patients who developed LEDVT were enrolled in the LEDVT group,and those who did not develop LEDVT were enrolled in the NLEDVT group.Demographic,clinical,and laboratory data were collected upon admission.Multivariable logistic regression analysis was performed to identify risk factors for LEDVT.Receiver operating characteristic(ROC)curve was used to evaluate the overall fit of the final model.Results There were 56 patients enrolled in the LEDVT group and 81 patients in the NLEDVT group.Age,Aggregate Index of Systemic Inflammation(AISI),Systemic Inflammation Response Index(SIRI),ICU length of stay,and albumin were identified as independent risk factors for LEDVT(all P<0.05).The area under their ROC curves were 0.604,0.657,0.694,0.668,and 0.405,respectively.Combined model for early clinical prediction of LEDVT in severely traumatized patients by age,SIRI,AISI,and albumin resulted in an area under the ROC curve of 0.805(95%CI:0.73-0.88,SE=0.037).Conclusion The combination of age,SIRI,AISI,and albumin has a predictive value for LEDVT in severely traumatized patients.展开更多
While incomplete non-medical data has been integrated into prediction models for epidemics,the accuracy and the generalizability of the data are difficult to guarantee.To comprehensively evaluate the ability and appli...While incomplete non-medical data has been integrated into prediction models for epidemics,the accuracy and the generalizability of the data are difficult to guarantee.To comprehensively evaluate the ability and applicability of using social media data to predict the development of COVID-19,a new confirmed case prediction algorithm improving the Google Flu Trends algorithm is established,called Weibo COVID-19 Trends(WCT),based on the post dataset generated by all users in Wuhan on Sina Weibo.A genetic algorithm is designed to select the keyword set for filtering COVID-19 related posts.WCT can constantly outperform the highest average test score in the training set between daily new confirmed case counts and the prediction results.It remains to produce the best prediction results among other algorithms when the number of forecast days increases from one to eight days with the highest correlation score from 0.98(P<0.01)to 0.86(P<0.01)during all analysis period.Additionally,WCT effectively improves the Google Flu Trends algorithm's shortcoming of overestimating the epidemic peak value.This study offers a highly adaptive approach for feature engineering of third-party data in epidemic prediction,providing useful insights for the prediction of newly emerging infectious diseases at an early stage.展开更多
Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The slructural evolution and morphological evolution of the milled powder were characterized b...Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The slructural evolution and morphological evolution of the milled powder were characterized by X-ray diffraction （XRD）, scanning electron mi- croscopy （SEM）, and energy dispersive spectroscopy （EDS） analysis. It is indicated that an appropriate amount of stearic acid accelerates the particle refinement process and favors the production of superfine Nb-based particles with good dispersivity and high activity. However, an inappropriate amount of stearic acid has an adverse effect on the refinement process.展开更多
AIM To investigate the evaluation of neogalactosylalbumin (NGA) for liver function assessment based on positron emission tomography technology. METHODS Female Kunming mice were assigned randomly to two groups: fibrosi...AIM To investigate the evaluation of neogalactosylalbumin (NGA) for liver function assessment based on positron emission tomography technology. METHODS Female Kunming mice were assigned randomly to two groups: fibrosis group and normal control group. A murine hepatic fibrosis model was generated by intraperitoneal injection of 10% carbon tetrachloride (CCl4) at 0.4 ml every 48 h for 42 d. F-18-labeled NGA ([F-18] FNGA) was synthesized and administered at a dosage of 3.7 MBq/mouse to both fibrosis mice and normal control mice. Distribution of [F-18] FNGA amongst organs was examined, and dynamic scanning was performed. Parameters were set up to compare the uptake of tracers by fibrotic liver and healthy liver. Serologic tests for liver function were also performed. RESULTS The liver function of the fibrosis model mice was significantly impaired by the use of CCl4. In the fibrosis model mice, hepatic fibrosis was verified by naked eye assessment and pathological analysis. [F-18] FNGA was found to predominantly accumulate in liver and kidneys in both control group (n = 21) and fibrosis group (n = 23). The liver uptake ability (LUA), peak time (T-p), and uptake rate (LUR) of [F-18] FNGA between healthy liver (n = 8) and fibrosis liver (n = 10) were significantly different (P < 0.05, < 0.01, and < 0.05, respectively). LUA was significantly correlated with total serum protein level (TP) (P < 0.05). T-p was significantly correlated with both TP and glucose (Glu) concentration (P < 0.05 both), and LUR was significantly correlated with both total bile acid and Glu concentration (P < 0.01 and < 0.05, respectively). CONCLUSION [F-18] FNGA mainly accumulated in liver and remained for sufficient time. Functionally-impaired liver showed a significant different uptake pattern of [F-18] FNGA compared to the controls.展开更多
Objectives To assess the clinical efficacy,safety,and feasibility of autologous transplantation of mobilized peripheral blood mononuclear cells(PBMNCs)for patients with peripheral arterial occlusive disease(PAOD)of th...Objectives To assess the clinical efficacy,safety,and feasibility of autologous transplantation of mobilized peripheral blood mononuclear cells(PBMNCs)for patients with peripheral arterial occlusive disease(PAOD)of the lower extremity.Methods A total of 152 patients with PAOD of the lower extremity were enrolled into this non-controlled observational study from November 2003 to March 2006.All patients received subcutaneous injections of recombinant human granulocyte colony-stimulating factor(G-CSF,450-600μg/day)for 5 days in order to mobilize stem/progenitor cells;their PBMNCs were collected and transplanted by multiple intramuscular injections into ischemic limbs.Patients were followed up for at least 12 weeks.Results At 12 weeks,primary manifestations,including lower limb pain and coldness,were significantly improved in 137(90.1%)of the patients;limb ulcers improved or healed in 46(86.8%)of the 53 patients,while 25 of the 48(47.9%)patients with limb gangrene remained steady or improved.Ankle-brachial index(ABI)improved in 33(22%)of the cases,and TcPO_(2) increased in 45(30%)of the cases.Angiography before treatment,and at 12 weeks after treatment,was performed in 10 of the patients and showed formation of new collateral vessels.No severe adverse effects or complications specifically related to cell transplantation were observed.Conclusion Autologous transplantation of G-CSF-mobilized PBMNCs might be a safe and effective treatment for lower limb ischemic disorder.展开更多
Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2...Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.展开更多
文摘Lunar wrinkle ridges are an important stress geological structure on the Moon, which reflect the stress state and geological activity on the Moon. They provide important insights into the evolution of the Moon and are key factors influencing future lunar activity, such as the choice of landing sites. However, automatic extraction of lunar wrinkle ridges is a challenging task due to their complex morphology and ambiguous features. Traditional manual extraction methods are time-consuming and labor-intensive. To achieve automated and detailed detection of lunar wrinkle ridges, we have constructed a lunar wrinkle ridge data set, incorporating previously unused aspect data to provide edge information, and proposed a Dual-Branch Ridge Detection Network(DBR-Net) based on deep learning technology. This method employs a dual-branch architecture and an Attention Complementary Feature Fusion module to address the issue of insufficient lunar wrinkle ridge features. Through comparisons with the results of various deep learning approaches, it is demonstrated that the proposed method exhibits superior detection performance. Furthermore, the trained model was applied to lunar mare regions, generating a distribution map of lunar mare wrinkle ridges;a significant linear relationship between the length and area of the lunar wrinkle ridges was obtained through statistical analysis, and six previously unrecorded potential lunar wrinkle ridges were detected. The proposed method upgrades the automated extraction of lunar wrinkle ridges to a pixel-level precision and verifies the effectiveness of DBR-Net in lunar wrinkle ridge detection.
基金supported by the National Natural Science Foundation of China(51874037)13th Five-Year Weapons Innovation Foundation of China(6141B012807)+1 种基金State Key Lab of Advanced Metals and Materials,University of Science and Technology Beijing(2019-Z14)the financial support for this research by the National Health and Medical Research Council(NHMRC),Australia through project grant(GNT1087290).
文摘A ternary Ti35Zr28Nb alloy was fabricated by powder metallurgy(PM)from pre-alloyed powder.The microstructure,hardness,corrosion behavior,and wear response of the produced alloy were investigated systematically.The results show that nearly full dense Ti35Zr28Nb alloy(relative density is 98.1±1.2%)can be fabricated by PM.The microstructure was dominated with uniform phase.The Ti35Zr28Nb alloy displayed spontaneous passivity in a naturally aerated simulated body fluid(SBF)solution at 37±0.5°C.The Ti35Zr28Nb alloy exhibited the highest corrosion resistance as compared to as-cast Ti6Al4V and pure Ti because of the formation of a protective passive film containing TiO2,Nb2O5,and ZrO2,including the highest corrosion potential(-0.22±0.01 V),the lowest corrosion current density(57.45±1.88 nA),the lowest passive potential(0.05±0.01 V)and the widest passivation range(1.29±0.09 V).Under the same wear condition,the wear rate of the Ti35Zr28Nb alloy(0.0021±0.0002 mm3/m·N)was lower than that of the CP Ti(0.0029±0.0004 mm3/m·N)and close to that of the Ti6Al4V(0.0020±0.0003 mm3/m·N).The wear mechanism of the Ti35Zr28Nb alloy was mainly dominated by abrasive wear,accompanied by adhesive wear.The highest corrosion resistance together with the adequate wear resistance makes the PM-fabricated Ti35Zr28Nb alloy an attractive candidate for orthopedic implant materials.
基金supported by the National High Level Hospital Clinical Research Fund (2022-PUMCH-B-109)CAMS Innovation Fund for Medical Sciences (CIFMS)(2021-1-I2M-020)。
文摘BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review aims to explore the development of evaluation animal models of SAE,giving insight into the direction of future research in terms of its pathophysiology and therapy.METHODS:We performed a literature search from January 1,2000,to December 31,2022,in MEDLINE,PubMed,EMBASE,and Web of Science using related keywords.Two independent researchers screened all the accessible articles based on the inclusion and exclusion criteria and collected the relevant data of the studies.RESULTS:The animal models for sepsis are commonly induced through cecal ligation and puncture(CLP) or lipopolysaccharide(LPS) injection.SAE can be evaluated using nervous reflex scores and sepsis evaluation during the acute phase,or through Morris water maze(MWM),openfield test,fear condition(FC) test,inhibitory avoidance,and other tests during the late phase.CONCLUSION:CLP and LPS injection are the most common methods for establishing SAE animal models.Nervous reflexs cores,MWM,FC test,and inhibitory avoidance are widely used in SAE model analysis.Future research should focus on establishing a standardized system for SAE development and analysis.
基金supported by grants from the National Key Technology Research and Development Program of China 2012(BAI06B01)the National Natural Science Foundation of China(81201566)+1 种基金the Specialized Research Fund for the Doctoral Program of Higher Education(20121106110002)Wu Jieping Medical Foundation(LDWMF-SY-2011B002)
文摘BACKGROUND： Golgi protein 73 （GP73） is a promising bio- marker of hepatocellular carcinoma （HCC）. It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effectiveness of the treatment. But changes of GP73 after transcatheter arterial chemoemboliza- tion （TACE） have not been reported so far. This study was to investigate the dynamic changes of GP73 in HCC patients af- ter TACE treatment, and the possible underlying mechanisms in the cell cultures. METHODS： Blood samples were collected from 72 HCC pa- tients, before TACE, at day I and day 30 after TACE. GP73 lev- els were measured by Western blotting. The dynamic changes of GP73 were analyzed and compared with image changes and clinical data. The effects of chemotherapeutic agents （5-FU and pirarubicin） on GP73 expression were tested in three HCC cell lines （HepG2, HCCLM3 and MHCC97H）. RESULTS： The GP73 level was significantly elevated at day 1 and day 30 after TACE in HCC patients compared with that before the procedure （P〈0.05）. There was no statistical differ- ence between the two time points after TACE, nor correlationbetween GP73 levels and dinicopathological features, tumor metastasis, and patient survival. Pirarubicin, not 5-FU, signifi- cantly increased GP73 expression in three cell lines. CONCLUSIONS： Unlike surgical resection which decreases the GP73 level, TACE significantly increased GP73 expression in patients with HCC. No correlations were observed among GP73 levels, tumor characteristics and prognosis of patients with HCC.
基金Supported by CAMS Innovation Fund for Medical Sciences,No.2016-I2M-1-001Tsinghua University-Peking Union Medical College Hospital Cooperation Project,No.PTQH201904552。
文摘BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism for the relationship between GPR and poor cancer prognosis remains unclear,studies have demonstrated the clinical effect of both gamma-glutamyl transferase and platelet count on GBC and related gallbladder diseases.AIM To assess the prognostic value of GPR and to design a prognostic nomogram for GBC.METHODS The analysis involved 130 GBC patients who underwent surgery at Peking Union Medical College Hospital from December 2003 to April 2017.The patients were stratified into a high-or low-GPR group.The predictive ability of GPR was evaluated by Kaplan–Meier analysis and a Cox regression model.We developed a nomogram based on GPR,which we verified using calibration curves.The nomogram and other prognosis prediction models were compared using timedependent receiver operating characteristic curves and the concordance index.RESULTS Patients in the high-GPR group had a higher risk of jaundice,were older,and had higher carbohydrate antigen 19-9 levels and worse postoperative outcomes.Univariate analysis revealed that GPR,age,body mass index,tumor–node–metastasis(TNM)stage,jaundice,cancer cell differentiation degree,and carcinoembryonic antigen and carbohydrate antigen 19-9 levels were related to overall survival(OS).Multivariate analysis confirmed that GPR,body mass index,age,and TNM stage were independent predictors of poor OS.Calibration curves were highly consistent with actual observations.Comparisons of timedependent receiver operating characteristic curves and the concordance index showed advantages for the nomogram over TNM staging.CONCLUSION GPR is an independent predictor of GBC prognosis,and nomogram-integrated GPR is a promising predictive model for OS in GBC.
基金supported by the Industry-Academia Cooperation Innovation Fund Project of Jiangsu Province(No.BY2011165)the Innovation Program of the Chinese Academy of Sciences(No.KZCX2-EW-314)+1 种基金the Key Project of"One Three Five"Strategic Developing Plan(No.NIGLAS2012135008)the National Water Pollution Control and Management Technology Major Project(No.2012ZX07103-005,2012ZX07101-010)
文摘Algae-caused black bloom （also known as black water agglomerate） has recently become a critical problem in some Chinese lakes.It has been suggested that the occurrence of algae-caused black bloom was caused by the cooperation of nutrient-rich sediment with dead algae,and sludge dredging was adopted to control black bloom in some lakes of China.In this article,based on the simulation of black bloom using a Y-shape apparatus for modeling natural conditions,both un-dredged and dredged sites in three areas of Taihu Lake,China were studied to estimate the effects of dredging on the prevention and control of black bloom.During the experiment,drained algae were added to all six sites as an additional organic load;subsequently,the dissolved oxygen decreased rapidly,dropping to 0 mg/L at the sediment-water interface.Black bloom did not occur in the dredged sites of Moon Bay and Nan Quan,whereas all three un-dredged sites at Fudu Port,Moon Bay and Nan Quan experienced black bloom.Black bloom also occurred at the dredged site of Fudu Port one day later than at the other sites,and the odor and color were lighter than at the other locations.The color and odor of the black water mainly result from the presence of sulfides such as metal sulfides and hydrogen sulfide,among other chemicals,under reductive conditions.The color and odor of the water,together with the high concentrations of nutrients,were mainly caused by the decomposition of the algae and the presence of nutrient-rich sediment.Overall,the removal of the nutrient-rich sediment by dredging can prevent the occurrence and control the degree of algae-caused black bloom in Taihu Lake.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.51874037 and 51922004)the Beijing Natural Science Foundation(No.2212035)+2 种基金the National Postdoctoral Program for Innovative Talents(No.BX20220034)the Fundamental Research Funds for the Central Universities(Nos.FRF-TP-19005C1Z and 00007718)the State Key Lab of Advanced Metals and Materials,University of Science and Technology Beijing(Nos.2021Z-03 and 2022Z-14).
文摘The challenge of producing a high-ductility titanium(Ti)material using inexpensive high-oxygen hydride-dehydride(HDH)Ti powder is hereby addressed by the incorporation of CaB_(6)oxygen-scavenger.The oxygen-scavenging behavior,microstructure evolution,mechanical behavior and improvement mechanism were systematically investigated.A continuous TiO_(2)oxide layer with a thickness of approximately 9.3 nm is presented on the HDH Ti powder surface.The oxide layer will dissolve into Ti matrix during sintering,making the increase of c/a value and leading to the Ti-Ti bonds developing from plastic metal bonds to-ward brittle covalent bonds.The CaB_(6)addition can scavenge O impurity and make a significant increase in tensile ductility forα-Ti matrix.A small addition of 0.2 wt.%CaB_(6)provides a superior tensile elongation of 22.2%for Ti material,almost three times as high as that of pure Ti(7.5%).The increase of defor-mation twining activity and grain refinement are responsible for the improved ductility.Furthermore,the CaB_(6)oxygen-scavenger can react with the surface oxide layer to in-situ form rod-like TiB and granular CaTiO_(3)reinforcements,refining the coarse near equiaxed grain ofα-Ti matrix into fine equiaxed crys-tals.The multiple mechanisms of grain-boundary strengthening,load-bearing strengthening of TiB and Orowan strengthening of CaTiO_(3)nanoparticles work together to increase the tensile strength of Ti/CaB_(6)composites.This work offers an effective method to fabricate high-performance Ti material using inex-pensive high-oxygen HDH Ti powder.
基金supported by the National Natural Science Foundation of China(No.52274359)Guangdong Basic and Applied Basic Research Foundation,China(No.2022A1515110406)+3 种基金Beijing Natural Science Foundation,China(No.2212035)the Fundamental Research Funds for the Central Universities,China(Nos.FRF-TP-19005C1Z and 00007718)the Aeroengine Group University Research Cooperation Project,China(No.HFZL2021CXY021)the State Key Lab of Advanced Metals and Materials,University of Science and Technology Beijing,China(Nos.2021Z-03 and 2022Z-14).
文摘Hot deformation of sintered billets by powder metallurgy(PM)is an effective preparation technique for titanium alloys,which is more significant for high-alloying alloys.In this study,Ti–6.5Al–2Zr–Mo–V(TA15)titanium alloy plates were prepared by cold press-ing sintering combined with high-temperature hot rolling.The microstructure and mechanical properties under different process paramet-ers were investigated.Optical microscope,electron backscatter diffraction,and others were applied to characterize the microstructure evolution and mechanical properties strengthening mechanism.The results showed that the chemical compositions were uniformly dif-fused without segregation during sintering,and the closing of the matrix craters was accelerated by increasing the sintering temperature.The block was hot rolled at 1200℃ with an 80%reduction under only two passes without annealing.The strength and elongation of the plate at 20–25℃ after solution and aging were 1247 MPa and 14.0%,respectively,which were increased by 24.5%and 40.0%,respect-ively,compared with the as-sintered alloy at 1300℃.The microstructure was significantly refined by continuous dynamic recrystalliza-tion,which was completed by the rotation and dislocation absorption of the substructure surrounded by low-angle grain boundaries.After hot rolling combined with heat treatment,the strength and plasticity of PM-TA15 were significantly improved,which resulted from the dense,uniform,and fine recrystallization structure and the synergistic effect of multiple slip systems.
基金funded and supported by the Department of Science and Technology of Guangdong Province(No.2022B1111020005)Key Laboratory of Guangdong Provincial Food and Drug Administration(No.2021ZDB03)+3 种基金the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund(Guangdong-Hong Kong-Macao Joint Lab,No.2020B1212030006)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(No.Guochao Liao,2019)Guangdong Basic and Applied Basic Research Foundation(Nos.2020B1515130005,2022A1515110270,202201011563)The Bureau of Science and Technology of Guangzhou City(No.HMJH_(2)019000)。
文摘Hepatocellular carcinoma is a common and fatal malignancy for which there is no effective systemic therapeutic strategy.Dihydroartemisinin(DHA),a derivative of artemisinin,has been shown to exert anti-tumor effects through the production of reactive oxygen species(ROS)and resultant mitochondrial damage.However,clinical translation is limited by several drawbacks,such as insolubility,instability and low bioavailability.Here,based on a nanomedicine-based delivery strategy,we fabricated mitochondria-targeted carrier-free nanoparticles coupling DHA and triphenylphosphonium(TPP),aiming to improve bioavailability and mitochondrial targeting.DHA-TPP nanoparticles can be passively delivered to the tumor site by enhanced penetration and retention and then internalized.Flow cytometry and Western blot analysis showed that DHA-TPP nanoparticles increased intracellular ROS,which increased mitochondrial stress and in turn upregulated the downstream Bcl-2 pathway,leading to apoptosis.In vivo experiments showed that DHA-TPP nanoparticles exhibited anti-tumor effects in a mouse model of hepatocellular carcinoma.These findings suggest carrier-free DHA-TPP nanoparticles as a potential therapeutic strategy for hepatocellular carcinoma.
基金Basic Research Spe-cial Project of Suzhou Science and Technology Bureau(SSD2024050).
文摘Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lower extremity deep venous thrombosis(LEDVT)in a severely traumatized population and to evaluate their predictive value for LEDVT.Methods This was a retrospective,single-center observational study.All subjects were severely traumatized patients who were admitted to the Traumatic Intensive Care Unit from January 2021 to May 2024.Based on Doppler ultrasound findings of both lower extremities from the time of injury to 30 days post-injury,patients who developed LEDVT were enrolled in the LEDVT group,and those who did not develop LEDVT were enrolled in the NLEDVT group.Demographic,clinical,and laboratory data were collected upon admission.Multivariable logistic regression analysis was performed to identify risk factors for LEDVT.Receiver operating characteristic(ROC)curve was used to evaluate the overall fit of the final model.Results There were 56 patients enrolled in the LEDVT group and 81 patients in the NLEDVT group.Age,Aggregate Index of Systemic Inflammation(AISI),Systemic Inflammation Response Index(SIRI),ICU length of stay,and albumin were identified as independent risk factors for LEDVT(all P<0.05).The area under their ROC curves were 0.604,0.657,0.694,0.668,and 0.405,respectively.Combined model for early clinical prediction of LEDVT in severely traumatized patients by age,SIRI,AISI,and albumin resulted in an area under the ROC curve of 0.805(95%CI:0.73-0.88,SE=0.037).Conclusion The combination of age,SIRI,AISI,and albumin has a predictive value for LEDVT in severely traumatized patients.
基金National Natural Science Foundation of China(Project No.:91846301,72025405,82041020,11975071,61773248,71771152)Sichuan Science and Technology Plan Project(Project No.:2020YFS0007)+2 种基金Hunan Science and Technology Plan Project(Project No.:2019GK2131,2020TP1013,2020JJ4673)Major Program of National Fund of Philosophy and Social Science of China(Project No.:18ZDA088,20ZDA060)Scientific Research Project of Shanghai Science and Technology Committee(Project No.:19511102202).
文摘While incomplete non-medical data has been integrated into prediction models for epidemics,the accuracy and the generalizability of the data are difficult to guarantee.To comprehensively evaluate the ability and applicability of using social media data to predict the development of COVID-19,a new confirmed case prediction algorithm improving the Google Flu Trends algorithm is established,called Weibo COVID-19 Trends(WCT),based on the post dataset generated by all users in Wuhan on Sina Weibo.A genetic algorithm is designed to select the keyword set for filtering COVID-19 related posts.WCT can constantly outperform the highest average test score in the training set between daily new confirmed case counts and the prediction results.It remains to produce the best prediction results among other algorithms when the number of forecast days increases from one to eight days with the highest correlation score from 0.98(P<0.01)to 0.86(P<0.01)during all analysis period.Additionally,WCT effectively improves the Google Flu Trends algorithm's shortcoming of overestimating the epidemic peak value.This study offers a highly adaptive approach for feature engineering of third-party data in epidemic prediction,providing useful insights for the prediction of newly emerging infectious diseases at an early stage.
基金the National Basic Research Program of China(No.2011CB606306)the National High Technology Research and Development Program of China(No.2009AA033201)+3 种基金the National Natural Science Foundation of China(No.50974017)the Fundamental Research Funds for the Central Universities(No.FRF-TP-11-004A)the Program for New Century Excellent Talentsin Universities of China(No.NCET-10-0226)Fok Ying Tung Education Foundation for Young College Teachers(No.122016)
文摘Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The slructural evolution and morphological evolution of the milled powder were characterized by X-ray diffraction （XRD）, scanning electron mi- croscopy （SEM）, and energy dispersive spectroscopy （EDS） analysis. It is indicated that an appropriate amount of stearic acid accelerates the particle refinement process and favors the production of superfine Nb-based particles with good dispersivity and high activity. However, an inappropriate amount of stearic acid has an adverse effect on the refinement process.
基金Supported by National Natural Science Foundation of China,No.30901453 and No.81201566National Key Technology Research and Development Program of China,No.BAI06B01Youth Grant of Peking Union Medical College Hospital
文摘AIM To investigate the evaluation of neogalactosylalbumin (NGA) for liver function assessment based on positron emission tomography technology. METHODS Female Kunming mice were assigned randomly to two groups: fibrosis group and normal control group. A murine hepatic fibrosis model was generated by intraperitoneal injection of 10% carbon tetrachloride (CCl4) at 0.4 ml every 48 h for 42 d. F-18-labeled NGA ([F-18] FNGA) was synthesized and administered at a dosage of 3.7 MBq/mouse to both fibrosis mice and normal control mice. Distribution of [F-18] FNGA amongst organs was examined, and dynamic scanning was performed. Parameters were set up to compare the uptake of tracers by fibrotic liver and healthy liver. Serologic tests for liver function were also performed. RESULTS The liver function of the fibrosis model mice was significantly impaired by the use of CCl4. In the fibrosis model mice, hepatic fibrosis was verified by naked eye assessment and pathological analysis. [F-18] FNGA was found to predominantly accumulate in liver and kidneys in both control group (n = 21) and fibrosis group (n = 23). The liver uptake ability (LUA), peak time (T-p), and uptake rate (LUR) of [F-18] FNGA between healthy liver (n = 8) and fibrosis liver (n = 10) were significantly different (P < 0.05, < 0.01, and < 0.05, respectively). LUA was significantly correlated with total serum protein level (TP) (P < 0.05). T-p was significantly correlated with both TP and glucose (Glu) concentration (P < 0.05 both), and LUR was significantly correlated with both total bile acid and Glu concentration (P < 0.01 and < 0.05, respectively). CONCLUSION [F-18] FNGA mainly accumulated in liver and remained for sufficient time. Functionally-impaired liver showed a significant different uptake pattern of [F-18] FNGA compared to the controls.
文摘Objectives To assess the clinical efficacy,safety,and feasibility of autologous transplantation of mobilized peripheral blood mononuclear cells(PBMNCs)for patients with peripheral arterial occlusive disease(PAOD)of the lower extremity.Methods A total of 152 patients with PAOD of the lower extremity were enrolled into this non-controlled observational study from November 2003 to March 2006.All patients received subcutaneous injections of recombinant human granulocyte colony-stimulating factor(G-CSF,450-600μg/day)for 5 days in order to mobilize stem/progenitor cells;their PBMNCs were collected and transplanted by multiple intramuscular injections into ischemic limbs.Patients were followed up for at least 12 weeks.Results At 12 weeks,primary manifestations,including lower limb pain and coldness,were significantly improved in 137(90.1%)of the patients;limb ulcers improved or healed in 46(86.8%)of the 53 patients,while 25 of the 48(47.9%)patients with limb gangrene remained steady or improved.Ankle-brachial index(ABI)improved in 33(22%)of the cases,and TcPO_(2) increased in 45(30%)of the cases.Angiography before treatment,and at 12 weeks after treatment,was performed in 10 of the patients and showed formation of new collateral vessels.No severe adverse effects or complications specifically related to cell transplantation were observed.Conclusion Autologous transplantation of G-CSF-mobilized PBMNCs might be a safe and effective treatment for lower limb ischemic disorder.
基金supported by the National Natural Science Foundation of China(No.81973762).
文摘Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.
