Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce...Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization.In this work,the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated.We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation,which could effectively upregulate osteogenesis-related factors and promote new bone formation,indicating the important role of macrophages polarization in biomaterial induction of osteogenesis.In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation.In addition,transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages,activating Toll-like receptor signaling pathway,which participated in the activation and attenuation of inflammation,and accelerated bone integration.Thus,by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release,the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration.展开更多
Aims:In this work,we evaluated the performance of three pitches positron emission tomography(PET)detectors for building a high-resolution small-animal PET imaging experimental platform.Methods:The evaluation of PET de...Aims:In this work,we evaluated the performance of three pitches positron emission tomography(PET)detectors for building a high-resolution small-animal PET imaging experimental platform.Methods:The evaluation of PET detectors includes twodimensional crystal identification,energy resolution,and crystal look-up table.The nuclear instrumentation module and PXI data acquisition(DAQ)system were both used to obtain flood images.The energy spectrum of the Na-22 source was measured.PET detectors of 2.0 and 1.6 mm pitches used in our present experiment were composed of lutetium yttrium oxyorthosilicate(LYSO)crystals directly coupled to position-sensitive photomultiplier tubes(PSPMTs).The 1.3-mm pitch detector comprises a LYSO crystal,a tapered light-guide,and a PSPMT.The crystal sizes of the detectors were a 10×10 array of 2.0×2.0×10 mm^(3) crystals,a 10×10 array of 1.6×1.6×10 mm^(3) crystals,and an 18×18 array of 1.3×1.3×10 mm^(3) crystals,respectively.Due to the lower sampling frequency of the DAQ system,a spline interpolation algorithm was introduced in the signal waveform to reconstruct the peak values.Results:The experimental results indicated that all three PET detector crystals could be clearly identified.A detailed analysis demonstrated that the key characteristic full width at half maximum(FWHM)values of the crystals profile could reach 0.46±0.03,0.32±0.03,and 0.21±0.02 mm,while the crystal average energy resolutions were 16.8%±1.76%,20.1%±2.24%,and 31.5%±3.76%,which correspond to pitches of 2.0-,1.6-,and 1.3-mm,respectively.Conclusions:The PET detector modules of LYSO crystal arrays could be potentially used in the small-animal PET imaging applications and are promising to achieve submillimeter PET images.展开更多
Background:Severe acute respiratory syndrome coronavirus 2 is a highly contagious viral infection,without any available targeted therapies.The high mortality rate of COVID-19 is speculated to be related to immune dama...Background:Severe acute respiratory syndrome coronavirus 2 is a highly contagious viral infection,without any available targeted therapies.The high mortality rate of COVID-19 is speculated to be related to immune damage.Methods:In this study,clinical bioinformatics analysis was conducted on transcriptome data of coronavirus infection.Results:Bioinformatics analysis revealed that the complex immune injury induced by coronavirus infection provoked dysfunction of numerous immune-related molecules and signaling pathways,including immune cells and toll-like receptor cascades.Production of numerous cytokines through the Th17 signaling pathway led to elevation in plasma levels of cytokines(including IL6,NF-kB,and TNF-a)followed by concurrent inflammatory storm,which mediates the autoimmune response.Several novel medications seemed to display therapeutic effects on immune damage associated with coronavirus infection.Conclusions:This study provided insights for further large-scale studies on the target therapy on reconciliation of immunological damage associated with COVID-19.展开更多
基金supported by the Science Foundation of Shandong Province of China[Grant No.ZR2021MH026,ZR2022MH075]Medicine and Health Science Technology Development plan of Shandong Province of China[Grant No.202108020440,2020Q127]+1 种基金Liaocheng Key Research and Development Plan of Shandong Province of China[Grant No.2022YDSF16,2022YDSF21]Liaocheng People’s Hospital Youth Fund Project[Grant No.LYQN201914].
文摘Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization.In this work,the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated.We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation,which could effectively upregulate osteogenesis-related factors and promote new bone formation,indicating the important role of macrophages polarization in biomaterial induction of osteogenesis.In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation.In addition,transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages,activating Toll-like receptor signaling pathway,which participated in the activation and attenuation of inflammation,and accelerated bone integration.Thus,by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release,the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration.
基金This work was supported by National Natural Science Foundation of China(11305083)in part by the Fundamental Research Funds for the Central Universities under Grant lzujbky-2016-27。
文摘Aims:In this work,we evaluated the performance of three pitches positron emission tomography(PET)detectors for building a high-resolution small-animal PET imaging experimental platform.Methods:The evaluation of PET detectors includes twodimensional crystal identification,energy resolution,and crystal look-up table.The nuclear instrumentation module and PXI data acquisition(DAQ)system were both used to obtain flood images.The energy spectrum of the Na-22 source was measured.PET detectors of 2.0 and 1.6 mm pitches used in our present experiment were composed of lutetium yttrium oxyorthosilicate(LYSO)crystals directly coupled to position-sensitive photomultiplier tubes(PSPMTs).The 1.3-mm pitch detector comprises a LYSO crystal,a tapered light-guide,and a PSPMT.The crystal sizes of the detectors were a 10×10 array of 2.0×2.0×10 mm^(3) crystals,a 10×10 array of 1.6×1.6×10 mm^(3) crystals,and an 18×18 array of 1.3×1.3×10 mm^(3) crystals,respectively.Due to the lower sampling frequency of the DAQ system,a spline interpolation algorithm was introduced in the signal waveform to reconstruct the peak values.Results:The experimental results indicated that all three PET detector crystals could be clearly identified.A detailed analysis demonstrated that the key characteristic full width at half maximum(FWHM)values of the crystals profile could reach 0.46±0.03,0.32±0.03,and 0.21±0.02 mm,while the crystal average energy resolutions were 16.8%±1.76%,20.1%±2.24%,and 31.5%±3.76%,which correspond to pitches of 2.0-,1.6-,and 1.3-mm,respectively.Conclusions:The PET detector modules of LYSO crystal arrays could be potentially used in the small-animal PET imaging applications and are promising to achieve submillimeter PET images.
基金supported by the 2017 National Geriatrics Clinical Medical Research Center Bidding Project(NCRCG-PLAGH-2017011)the Translational Medicine Project of Chinese PLA General Hospital(2017TM-020)+2 种基金Pudong New Area Health and Health Committee Subject Leader Program(PWRd2019-04)Pudong New Area TCM Peak Disease Subject(PDZY-2018-0603)the Innovation Project of Chinese PLA General Hospital(CX19028).
文摘Background:Severe acute respiratory syndrome coronavirus 2 is a highly contagious viral infection,without any available targeted therapies.The high mortality rate of COVID-19 is speculated to be related to immune damage.Methods:In this study,clinical bioinformatics analysis was conducted on transcriptome data of coronavirus infection.Results:Bioinformatics analysis revealed that the complex immune injury induced by coronavirus infection provoked dysfunction of numerous immune-related molecules and signaling pathways,including immune cells and toll-like receptor cascades.Production of numerous cytokines through the Th17 signaling pathway led to elevation in plasma levels of cytokines(including IL6,NF-kB,and TNF-a)followed by concurrent inflammatory storm,which mediates the autoimmune response.Several novel medications seemed to display therapeutic effects on immune damage associated with coronavirus infection.Conclusions:This study provided insights for further large-scale studies on the target therapy on reconciliation of immunological damage associated with COVID-19.
