Poly(ester amide)s(PEAs)represent promising biomaterials because of their well-balanced mechanical properties,biodegradability,and biocompatibility.However,practical applications of PEAs are still limited by challenge...Poly(ester amide)s(PEAs)represent promising biomaterials because of their well-balanced mechanical properties,biodegradability,and biocompatibility.However,practical applications of PEAs are still limited by challenges in functional versatility and environmental adaptability.Here,we present the first synthesis of periodic selenium-incorporated PEAs(Se-PEAs)via a rapid,catalyst-free selenol-yne click polymerization process.By harnessing the versatility of selenium,we achieved precise modulation of material properties.The resulting Se-PEAs demonstrated tunable mechanical behavior,spanning rigid plastics to elastomers,alongside exceptional thermal stability and high optical clarity.Programmable degradation profiles ensure long-term stability in physiological environments while facilitating rapid oxidative degradation at the end of the lifecycle.Surface selenoniumization further conferred robust antibacterial efficacy without compromising mechanical integrity.This multifunctionality positions Se-PEAs as transformative materials for biomedical implants,sustainable packaging,and high-refractiveindex optics.Our work advanced functional polymer design and underscored the potential of selenium chemistry in addressing global challenges in terms of plastic waste and ecological sustainability.展开更多
The limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry.An example of this is camptothecin,a monoterpene indole alkaloid with potent...The limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry.An example of this is camptothecin,a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants.Recently,camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila.Here,time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O.pumila.It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation.Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels.Accordingly,silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant.Further detailed molecular characterization by electrophoretic mobility shift,yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC.Taken together,the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis.As such,a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.展开更多
INTRODUCTION Pentatrichomonas hominis,an anaerobic flagellated protozoan that inhabits the large intestines of mammals and belongs to the Trichomonadidae family(Wenrich 1944;Kim et al.2010;Li et al.2014b,2016,2018a,20...INTRODUCTION Pentatrichomonas hominis,an anaerobic flagellated protozoan that inhabits the large intestines of mammals and belongs to the Trichomonadidae family(Wenrich 1944;Kim et al.2010;Li et al.2014b,2016,2018a,2020;Maritz et al.2014;Zhang et al.2019),is mainly transmitted through the fecal-oral route.It was originally presumed to be a commensal protozoan(Tolbert et al.2012)but was found to cause gastrointestinal symptoms.展开更多
基金supported by the National Natural Science Foundation of China(21971177)the Natural Science Foundation of the Jiangsu Higher Education Institution of China(22KJA150004)+3 种基金the Suzhou Science and Technology Bureau(SZM2021008)the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutionsthe Jiangsu Key Laboratory of Advanced Functional Polymers Design and Application,Soochow University,Suzhou Medical and Industrial Cooperation Innovation Project(SZM2022011)the Suzhou Key Laboratory of Macromolecular Design and Precision Synthesis and the Program of Innovative Research Team of Soochow University。
文摘Poly(ester amide)s(PEAs)represent promising biomaterials because of their well-balanced mechanical properties,biodegradability,and biocompatibility.However,practical applications of PEAs are still limited by challenges in functional versatility and environmental adaptability.Here,we present the first synthesis of periodic selenium-incorporated PEAs(Se-PEAs)via a rapid,catalyst-free selenol-yne click polymerization process.By harnessing the versatility of selenium,we achieved precise modulation of material properties.The resulting Se-PEAs demonstrated tunable mechanical behavior,spanning rigid plastics to elastomers,alongside exceptional thermal stability and high optical clarity.Programmable degradation profiles ensure long-term stability in physiological environments while facilitating rapid oxidative degradation at the end of the lifecycle.Surface selenoniumization further conferred robust antibacterial efficacy without compromising mechanical integrity.This multifunctionality positions Se-PEAs as transformative materials for biomedical implants,sustainable packaging,and high-refractiveindex optics.Our work advanced functional polymer design and underscored the potential of selenium chemistry in addressing global challenges in terms of plastic waste and ecological sustainability.
基金the National Key Research and Development Program of China(2018YFC1706203)National Natural Science Foundation of China(31571735,82073963,81522049,82003889)+3 种基金Zhejiang Provincial Ten Thousands Program for Leading Talents of Science and Technology Innovation(2018R52050)Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents,Shanghai Science and Technology Committee Project(17JC1404300)Zhejiang Provincial Natural Science Foundation of China(LY20H280008,LQ21H280004)Zhejiang Chinese Medical University Research Foundation(2020ZR15).
文摘The limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry.An example of this is camptothecin,a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants.Recently,camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila.Here,time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O.pumila.It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation.Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels.Accordingly,silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant.Further detailed molecular characterization by electrophoretic mobility shift,yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC.Taken together,the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis.As such,a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.
基金This work was supported by the grants from the National Natural Science Foundation of China(No.32102696)the National Key Research and Development Program of China(No.2021YFF0702900).
文摘INTRODUCTION Pentatrichomonas hominis,an anaerobic flagellated protozoan that inhabits the large intestines of mammals and belongs to the Trichomonadidae family(Wenrich 1944;Kim et al.2010;Li et al.2014b,2016,2018a,2020;Maritz et al.2014;Zhang et al.2019),is mainly transmitted through the fecal-oral route.It was originally presumed to be a commensal protozoan(Tolbert et al.2012)but was found to cause gastrointestinal symptoms.
