In this study,chitosan(CS)was combined with microcrystalline cellulose(MCC)to fabricate composite hydrogel beads.These beads were further modified through blending and grafting with polyethyleneimine(PEI)to develop ch...In this study,chitosan(CS)was combined with microcrystalline cellulose(MCC)to fabricate composite hydrogel beads.These beads were further modified through blending and grafting with polyethyleneimine(PEI)to develop chitosan/microcrystalline cellulose@polyethyleneimine(CS/MCC@PEI)composite gel spheres for the efficient adsorption of diclofenac sodium(DS)from aqueous solutions.The adsorbent was characterized using scanning electron microscopy(SEM),X-ray diffraction(XRD),Fourier-transform infrared spectroscopy(FTIR),X-ray pho-toelectron spectroscopy(XPS),and thermogravimetric analysis(TGA).The CS/MCC@PEI composite exhibited a spherical morphology with a porous structure,abundant surface functional groups,and a high adsorption capac-ity of 274.84 mg/g for DS.Kinetic studies revealed that the adsorption process followed the pseudo-second-order model,dominated by physical adsorption,with both surface and internal diffusion influencing the adsorption rate.The Freundlich isotherm model best described the adsorption behavior,indicating multilayer adsorption on heterogeneous surfaces.Environmental adaptability tests demonstrated minimal interference from co-existing anions and humic acid,while regeneration experiments confirmed excellent reusability(>77%removal after five cycles).The adsorption mechanism involved electrostatic interactions and hydrogen bonding between the hydroxyl/amino groups of the composite and DS.These findings highlight the potential of CS/MCC@PEI as a cost-effective and sustainable adsorbent for DS removal from water.展开更多
Grape white rot,a devastating disease of grapevines caused by Coniella diplodiella(Speg.)Sacc.,leads to significant yield losses in grape.Breeding grape cultivars resistant to white rot is essential to reduce the regu...Grape white rot,a devastating disease of grapevines caused by Coniella diplodiella(Speg.)Sacc.,leads to significant yield losses in grape.Breeding grape cultivars resistant to white rot is essential to reduce the regular use of chemical treatments.In recent years,Chinese grape species have gained more attention for grape breeding due to their high tolerance to various biotic and abiotic factors along with changing climatic conditions.In this study,we employed whole-genome resequencing(WGR)to genotype the parents of‘Manicure Finger’(Vitis vinifera,female)and‘0940’(Vitis davidii,male),along with 101 F1 mapping population individuals,thereby constructing a linkage genetic map.The linkage map contained 9337 single-nucleotide polymorphism(SNP)markers with an average marker distance of 0.3 cM.After 3 years of phenotypic evaluation of the progeny for white rot resistance,we confirmed one stable quantitative trait locus(QTL)for white rot resistance on chromosome 3,explaining up to 17.9%of the phenotypic variation.For this locus,we used RNA-seq to detect candidate gene expression and identified PR1 as a candidate gene involved in white rot resistance.Finally,we demonstrated that recombinant PR1 protein could inhibit the growth of C.diplodiella and that overexpression of PR1 in susceptible V.vinifera increased grape resistance to the pathogen.展开更多
As a recently emerging wastewater treatment technology,Algal-bacterial granular sludge(ABGS)process shows significant advantages.However,current research on the ABGS system is a lack of a clear and complete understand...As a recently emerging wastewater treatment technology,Algal-bacterial granular sludge(ABGS)process shows significant advantages.However,current research on the ABGS system is a lack of a clear and complete understanding of the potential mechanism of signal molecules on the growth of ABGS.This study comprehensively explores the variations in the ABGS under different N-acyl-homoserine lactone(AHL)conditions by constructing three sequencing batch reactor(SBR)systems.The results indicate that N-hexanoyl-l-homoserine lactone(C6-HSL)accelerates the granulation process in the early stages by promoting the loosely bound extracellular polymeric substances(LB-EPS)secretion and filamentous bacteria growth,thereby shortening required time for initial granule formation.On the other hand,N-(3-oxodecanoyl)-l-homoserine lactone(3-oxo-C12-HSL)expedites the granulation process by promoting the tightly bound extracellular polymeric substances(TB-EPS)and aromatic protein secretion,benefiting structural stability and nitrogen and phosphorus removal efficiency of mature ABGS.展开更多
基金supported by the Open Project Funding of Key Laboratory of Intelligent Health Perception and Ecological Restoration of Rivers and Lakes,Ministry of Education,Hubei University of Technology(No.HGKFZ03).
文摘In this study,chitosan(CS)was combined with microcrystalline cellulose(MCC)to fabricate composite hydrogel beads.These beads were further modified through blending and grafting with polyethyleneimine(PEI)to develop chitosan/microcrystalline cellulose@polyethyleneimine(CS/MCC@PEI)composite gel spheres for the efficient adsorption of diclofenac sodium(DS)from aqueous solutions.The adsorbent was characterized using scanning electron microscopy(SEM),X-ray diffraction(XRD),Fourier-transform infrared spectroscopy(FTIR),X-ray pho-toelectron spectroscopy(XPS),and thermogravimetric analysis(TGA).The CS/MCC@PEI composite exhibited a spherical morphology with a porous structure,abundant surface functional groups,and a high adsorption capac-ity of 274.84 mg/g for DS.Kinetic studies revealed that the adsorption process followed the pseudo-second-order model,dominated by physical adsorption,with both surface and internal diffusion influencing the adsorption rate.The Freundlich isotherm model best described the adsorption behavior,indicating multilayer adsorption on heterogeneous surfaces.Environmental adaptability tests demonstrated minimal interference from co-existing anions and humic acid,while regeneration experiments confirmed excellent reusability(>77%removal after five cycles).The adsorption mechanism involved electrostatic interactions and hydrogen bonding between the hydroxyl/amino groups of the composite and DS.These findings highlight the potential of CS/MCC@PEI as a cost-effective and sustainable adsorbent for DS removal from water.
基金This studywas funded by the National Key Research and Development Program of China(2021YFD1200200)the National Natural Science Foundation of China(31872057)+1 种基金the China Agriculture Research System(CARS-29)the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2021-ZFRI).
文摘Grape white rot,a devastating disease of grapevines caused by Coniella diplodiella(Speg.)Sacc.,leads to significant yield losses in grape.Breeding grape cultivars resistant to white rot is essential to reduce the regular use of chemical treatments.In recent years,Chinese grape species have gained more attention for grape breeding due to their high tolerance to various biotic and abiotic factors along with changing climatic conditions.In this study,we employed whole-genome resequencing(WGR)to genotype the parents of‘Manicure Finger’(Vitis vinifera,female)and‘0940’(Vitis davidii,male),along with 101 F1 mapping population individuals,thereby constructing a linkage genetic map.The linkage map contained 9337 single-nucleotide polymorphism(SNP)markers with an average marker distance of 0.3 cM.After 3 years of phenotypic evaluation of the progeny for white rot resistance,we confirmed one stable quantitative trait locus(QTL)for white rot resistance on chromosome 3,explaining up to 17.9%of the phenotypic variation.For this locus,we used RNA-seq to detect candidate gene expression and identified PR1 as a candidate gene involved in white rot resistance.Finally,we demonstrated that recombinant PR1 protein could inhibit the growth of C.diplodiella and that overexpression of PR1 in susceptible V.vinifera increased grape resistance to the pathogen.
基金financially supported by the Open Project of Sanya Science and Education Innovation Park of Wuhan University of Technology(No.2022KF0005)Researchers Supporting Project(No.RSP-2024-R20)King Saud University,Riyadh,Saudi Arabia.
文摘As a recently emerging wastewater treatment technology,Algal-bacterial granular sludge(ABGS)process shows significant advantages.However,current research on the ABGS system is a lack of a clear and complete understanding of the potential mechanism of signal molecules on the growth of ABGS.This study comprehensively explores the variations in the ABGS under different N-acyl-homoserine lactone(AHL)conditions by constructing three sequencing batch reactor(SBR)systems.The results indicate that N-hexanoyl-l-homoserine lactone(C6-HSL)accelerates the granulation process in the early stages by promoting the loosely bound extracellular polymeric substances(LB-EPS)secretion and filamentous bacteria growth,thereby shortening required time for initial granule formation.On the other hand,N-(3-oxodecanoyl)-l-homoserine lactone(3-oxo-C12-HSL)expedites the granulation process by promoting the tightly bound extracellular polymeric substances(TB-EPS)and aromatic protein secretion,benefiting structural stability and nitrogen and phosphorus removal efficiency of mature ABGS.
