Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progres...Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progressive disease deterioration and increased severity.Nevertheless,the molecular mechanisms underlying dengue-associated neutropenia remain inadequately elucidated.In this study,the comprehensive plasma proteomic profiling of dengue fever(DF)patients,DF patients with neutropenia(DFN),and healthy controls(HC)was systematically analyzed using a deep dataindependent acquisition(DIA)workflow combined with LC-MS/MS analysis,to elucidate key cellular pathways and identify promising biomarkers.DFN patients exhibited significant dual hematological alterations,with notable changes in both platelet and neutrophil counts,reflecting a complex disturbance in hematological homeostasis during dengue progression.DIA analysis quantified 2475 proteins,revealing widespread proteomic alterations among the DF,DFN,and HC subjects.Differential analysis highlighted significant fluctuations in proteins related to cytoskeletal organization,metabolic regulation,and intracellular signaling.Enrichment analyses implicated pathways such as focal adhesion,platelet activation,and PI3K-Akt signaling.Machine learning methods further identified a panel of four biomarkers-CNST,DSTN,DUSP3,and PDIA5-with high predictive accuracy for dengue diagnosis and subgroup differentiation.In conclusion,this study advances our understanding of dengue’s plasma proteomic landscape and underscores the synergistic potential of DIA-based proteomics and machine learning in unveiling host-response mechanisms,thereby informing early diagnosis and targeted therapeutic strategies.展开更多
Different switching frequencies are required when SiC metal-oxide-semiconductor field-effect transistors(MOSFETs)are switching in a space environment.In this study,the total ionizing dose(TID)responses of SiC power MO...Different switching frequencies are required when SiC metal-oxide-semiconductor field-effect transistors(MOSFETs)are switching in a space environment.In this study,the total ionizing dose(TID)responses of SiC power MOSFETs are investigated under different switching frequencies from 1 kHz to 10 MHz.A significant shift was observed in the threshold voltage as the frequency increased,which resulted in premature failure of the drain-source breakdown voltage and drain-source leakage current.The degradation is attributed to the high activation and low recovery rates of traps at high frequencies.The results of this study suggest that a targeted TID irradiation test evaluation method can be developed according to the actual switching frequency of SiC power MOSFETs.展开更多
Tetranychus urticae Koch;Insecticide resistance;Chemical control This paper elaborates the occurrence factors and damage characteristics of Tetranychus urticae Koch in China,and emphatically summarizes three main cont...Tetranychus urticae Koch;Insecticide resistance;Chemical control This paper elaborates the occurrence factors and damage characteristics of Tetranychus urticae Koch in China,and emphatically summarizes three main control strategies of T.urticae,namely agricultural control,chemical control and biological control,as well as research progress in its resistance mechanisms.The problems existing in controlling T.urticae and its resistance management strategies are put forward,to provide a theoretical basis for the resistance management and comprehensive control of T.urticae.展开更多
Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric p...Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric probes with zero probe-product crosstalk remains challenging.Harnessing the recent progress on photophysical modulation of xanthenoid fluorochromes,we propose a powerful and versatile probe design principle,that is,“bridging-group modification,”and developed totalROX,a robust probe for monitoring the total cellular oxidative capacity.展开更多
基金supported by National Key R&D Program of China(2023YFA0915600)Guangdong Basic and Applied Basic Research Foundation(2025B1515020010)+3 种基金Shenzhen Clinical Research Center for Emerging Infectious Diseases(LCYSSQ20220823091203007)Sanming Project of Medicine in Shenzhen(SZSM202311033)Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties(No.SZGSP011)Shenzhen High-level Hospital Construction Fund(XKJSCRGRK-006).
文摘Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progressive disease deterioration and increased severity.Nevertheless,the molecular mechanisms underlying dengue-associated neutropenia remain inadequately elucidated.In this study,the comprehensive plasma proteomic profiling of dengue fever(DF)patients,DF patients with neutropenia(DFN),and healthy controls(HC)was systematically analyzed using a deep dataindependent acquisition(DIA)workflow combined with LC-MS/MS analysis,to elucidate key cellular pathways and identify promising biomarkers.DFN patients exhibited significant dual hematological alterations,with notable changes in both platelet and neutrophil counts,reflecting a complex disturbance in hematological homeostasis during dengue progression.DIA analysis quantified 2475 proteins,revealing widespread proteomic alterations among the DF,DFN,and HC subjects.Differential analysis highlighted significant fluctuations in proteins related to cytoskeletal organization,metabolic regulation,and intracellular signaling.Enrichment analyses implicated pathways such as focal adhesion,platelet activation,and PI3K-Akt signaling.Machine learning methods further identified a panel of four biomarkers-CNST,DSTN,DUSP3,and PDIA5-with high predictive accuracy for dengue diagnosis and subgroup differentiation.In conclusion,this study advances our understanding of dengue’s plasma proteomic landscape and underscores the synergistic potential of DIA-based proteomics and machine learning in unveiling host-response mechanisms,thereby informing early diagnosis and targeted therapeutic strategies.
基金supported by the National Natural Science Foundation of China under Grant No.11975305the West Light Foundation of The Chinese Academy of Sciences,Grant No.2017-XBQNXZ-B-008。
文摘Different switching frequencies are required when SiC metal-oxide-semiconductor field-effect transistors(MOSFETs)are switching in a space environment.In this study,the total ionizing dose(TID)responses of SiC power MOSFETs are investigated under different switching frequencies from 1 kHz to 10 MHz.A significant shift was observed in the threshold voltage as the frequency increased,which resulted in premature failure of the drain-source breakdown voltage and drain-source leakage current.The degradation is attributed to the high activation and low recovery rates of traps at high frequencies.The results of this study suggest that a targeted TID irradiation test evaluation method can be developed according to the actual switching frequency of SiC power MOSFETs.
基金Supported by Guangxi Agricultural Science and Technology Self-financing Project(Z2022128)Fund Project of Guangxi Citrus Breeding and Cultivation Engineering Technology Research Center(2023A001).
文摘Tetranychus urticae Koch;Insecticide resistance;Chemical control This paper elaborates the occurrence factors and damage characteristics of Tetranychus urticae Koch in China,and emphatically summarizes three main control strategies of T.urticae,namely agricultural control,chemical control and biological control,as well as research progress in its resistance mechanisms.The problems existing in controlling T.urticae and its resistance management strategies are put forward,to provide a theoretical basis for the resistance management and comprehensive control of T.urticae.
基金supported by the National Natural Science Foundation of China(nos.21822805,21908065,31871430,81802979,818800585,and 8180032537)Young Medical Talents Training Program of Shanghai(2018)+3 种基金the Commission of Science and Technology of Shanghai Municipality(no.18430711000)the Australian National Health and Medical Research Council(nos.APP1126091 and APP1125794)the Science and Technology Commission of Shanghai Municipality for the Shanghai International Cooperation Program(18430711000)the China Postdoctoral Science Foundation(nos.2019M651427 and 2020T130197),W.Yang acknowledges the financial support of the innovative research team of high-level local universities in Shanghai.
文摘Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric probes with zero probe-product crosstalk remains challenging.Harnessing the recent progress on photophysical modulation of xanthenoid fluorochromes,we propose a powerful and versatile probe design principle,that is,“bridging-group modification,”and developed totalROX,a robust probe for monitoring the total cellular oxidative capacity.
