High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases.Current non-invasive neuromodulation methods offer limited precision at the milimeter level.Here,we report op...High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases.Current non-invasive neuromodulation methods offer limited precision at the milimeter level.Here,we report opticallygenerated focused ultrasound(OFUS)for non-invasive brain stimulation with ultrahigh precision.OFUS is generated by a soft optoacoustic pad(SOAP)fabricated through embedding candle soot nanoparticles in a curved polydimethylsiloxane film.SOAP generates a transcranial ultrasound focus at 15 MHz with an ultrahigh lateral resolution of 83μm,which is two orders of magnitude smaller than that of conventional transcranial-focused ultrasound(tFUS).Here,we show effective OFUS neurostimulation in vitro with a single ultrasound cycle.We demonstrate submillimeter transcranial stimulation of the mouse motor cortex in vivo.An acoustic energy of 0.6 mJ/cm?,four orders of magnitude less than that of tFUS,is suffcient for successful OFUS neurostimulation.OFUS offers new capabilities for neuroscience studies and disease treatments by delivering a focus with ultrahigh precision noninvasively.展开更多
Stimulated Raman scattering(SRS)microscopy is a highly sensitive chemical imaging technique.However,the SRS imaging performance hinges on two key factors:the reliance on low-noise but bulky solid-state laser sources a...Stimulated Raman scattering(SRS)microscopy is a highly sensitive chemical imaging technique.However,the SRS imaging performance hinges on two key factors:the reliance on low-noise but bulky solid-state laser sources and stringent sample requirements necessitated by high numerical aperture(NA)optics.Here,we present a fiber laser based stimulated Raman photothermal(SRP)microscope that addresses these limitations.While appreciating the portability and compactness of a noisy source,fiber laser SRP enables a two-order-of-magnitude improvement in signal to noise ratio over fiber laser SRS without balance detection.Furthermore,with the use of low NA,long working distance optics for signal collection,SRP expands the allowed sample space from millimeters to centimeters,which diversifies the sample formats to multiwell plates and thick tissues.The sensitivity and imaging depth are further amplified by using urea for both thermal enhancement and tissue clearance.Together,fiber laser SRP microscopy provides a robust,user-friendly platform for diverse applications.展开更多
基金This work is supported by R01 NS109794 to J.X.C.and C.Y.and R01 HL 125385 to J.-X.CResearch reported in this publication was supported by the Boston University Micro and Nano Imaging Facility and the Office of the Director,National Institutes of Health of the National Institutes of Health under award Number S100D024993.
文摘High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases.Current non-invasive neuromodulation methods offer limited precision at the milimeter level.Here,we report opticallygenerated focused ultrasound(OFUS)for non-invasive brain stimulation with ultrahigh precision.OFUS is generated by a soft optoacoustic pad(SOAP)fabricated through embedding candle soot nanoparticles in a curved polydimethylsiloxane film.SOAP generates a transcranial ultrasound focus at 15 MHz with an ultrahigh lateral resolution of 83μm,which is two orders of magnitude smaller than that of conventional transcranial-focused ultrasound(tFUS).Here,we show effective OFUS neurostimulation in vitro with a single ultrasound cycle.We demonstrate submillimeter transcranial stimulation of the mouse motor cortex in vivo.An acoustic energy of 0.6 mJ/cm?,four orders of magnitude less than that of tFUS,is suffcient for successful OFUS neurostimulation.OFUS offers new capabilities for neuroscience studies and disease treatments by delivering a focus with ultrahigh precision noninvasively.
基金supported by NIH grants R35GM136223, R01EB032391, R01EB035429 to JXC.
文摘Stimulated Raman scattering(SRS)microscopy is a highly sensitive chemical imaging technique.However,the SRS imaging performance hinges on two key factors:the reliance on low-noise but bulky solid-state laser sources and stringent sample requirements necessitated by high numerical aperture(NA)optics.Here,we present a fiber laser based stimulated Raman photothermal(SRP)microscope that addresses these limitations.While appreciating the portability and compactness of a noisy source,fiber laser SRP enables a two-order-of-magnitude improvement in signal to noise ratio over fiber laser SRS without balance detection.Furthermore,with the use of low NA,long working distance optics for signal collection,SRP expands the allowed sample space from millimeters to centimeters,which diversifies the sample formats to multiwell plates and thick tissues.The sensitivity and imaging depth are further amplified by using urea for both thermal enhancement and tissue clearance.Together,fiber laser SRP microscopy provides a robust,user-friendly platform for diverse applications.
