tract in external use on expression of proto-onco- genes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preve...tract in external use on expression of proto-onco- genes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia. METHODS Thirty virginal female Wistar rats were randomized into 5 groups, 6 in each, A： blank con- trol group; B： model group; C： the low dose group of Rupifang; D： the middle dose group of Rupifang; and E： The high dose group of Rupifang. The mam- mary hyperplasia rat models were produced by in- jecting estradiol benzoate and progesterone and ir- ritating by tail nipping. Drug intervention was also launched during the model formation. After 30 days, the expression of her2 and p16 in breast tis- sues of rats in each group were detected by the SP immunohistochemical method. RESULTS： Compared with Blank control group, the expression of her2 in breast tissues in Model group was higher, and the expression of p16 was lower （P〈O.05 or P〈O.01）. After intervention with Rupi- fang Extract, compared with Model group, the ex- pression of her2 in breast tissues in Rupifang groups was lower, and the expression of p16 higher （P〈O.05 or P〈O.01）. CONCLUSION： The mechanisms of Rupifang Ex- tract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and in- creasing the expression of tumor suppression genes p16.展开更多
基金Supported by China National Foundation of Natural Science(Project No.81173265)Foundation of Natural Science of Guangdong Province(Project No.10151063201000065)+11 种基金Science and Technology Plan Projects of Guangdong Province(No:2009B0308012382012B031800155)The Fundamental Research Funds for the Central Universities (No.21612422216113119)Guangzhou Municipal Planned Science and Technology Project(No.2009Z1-E091)Guangdong Provincial Administration of Traditional Chinese Medicine(No.201111752008092)Guangdong University Students' Innovation Experimental Program(No.1055910014)Jinan University's the National Collegiate Innovation Experimental Program,2010(No.101055916)Jinan University's Cultivation Project of Scientific Research Creation for Outstanding Undergraduates Recommended for Post-graduate StudyJinan University's the National Collegiate Innovation and Startups Training Program(No.1210559029)Jinan University's 211 Engineering Construction Program
文摘tract in external use on expression of proto-onco- genes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia. METHODS Thirty virginal female Wistar rats were randomized into 5 groups, 6 in each, A： blank con- trol group; B： model group; C： the low dose group of Rupifang; D： the middle dose group of Rupifang; and E： The high dose group of Rupifang. The mam- mary hyperplasia rat models were produced by in- jecting estradiol benzoate and progesterone and ir- ritating by tail nipping. Drug intervention was also launched during the model formation. After 30 days, the expression of her2 and p16 in breast tis- sues of rats in each group were detected by the SP immunohistochemical method. RESULTS： Compared with Blank control group, the expression of her2 in breast tissues in Model group was higher, and the expression of p16 was lower （P〈O.05 or P〈O.01）. After intervention with Rupi- fang Extract, compared with Model group, the ex- pression of her2 in breast tissues in Rupifang groups was lower, and the expression of p16 higher （P〈O.05 or P〈O.01）. CONCLUSION： The mechanisms of Rupifang Ex- tract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and in- creasing the expression of tumor suppression genes p16.
