Zur(zinc uptake regulator),a member of the Fur(ferric uptake regulator)family of transcriptional regulators,plays multifaceted roles by regulating the gene expressions,such as modulating zinc ion uptake by regulating ...Zur(zinc uptake regulator),a member of the Fur(ferric uptake regulator)family of transcriptional regulators,plays multifaceted roles by regulating the gene expressions,such as modulating zinc ion uptake by regulating the znuABC gene cluster and influencing bacterial motility by modulating genes associated with flagella or pili.The photosynthetic autotroph Synechocystis sp.PCC 6803 is frequently used as an indicator organism for water pollution and a cell factory for high-value biochemical production in synthetic biology.During its growth,this organism often encounters various abiotic stresses,including oxidative,salt,and antibiotic stress.In this study,we conducted transcriptomic analysis on bothΔzur mutant and wild-type(WT)strains to identify potential Zur-regulated genes in Synechocystis sp.PCC 6803.These genes primarily participate in multiple pathways such as inorganic ion transport,carbohydrate transport,energy production and conversion,and cell motility.Zur not only controls zinc ion homeostasis within the cell but also influences the iron balance by directly regulating the expression of the fur gene.In terms of motility,Zur regulates the expression of bacterial pili gene cluster and other motility-related genes,thereby affecting the twitching motility of Synechocystis sp.PCC 6803.Furthermore,Zur plays a crucial role in promoting biofilm formation and enhancing resistance to salt,oxidative,and antibiotic stresses by modulating relative gene expression.In conclusion,as a global transcriptional regulator,Zur plays pivotal roles in metal ion homeostasis,motility,and resistance to multiple stresses in Synechocystis sp.PCC 6803.This study illustrates the Zur regulons in Synechocystis sp.PCC 6803,and underscores the importance of Zur in enhancing the environmental adaptability of cyanobacteria.Highlights 1.Transcriptome analysis of Zur’s regulons inSynechocystis sp.PCC 6803.2.Zur mediates metal homeostasis beyond zinc.3.Zur modulates pili biosynthesis and subsequent motility.4.Zur promotes biofilm formation and stresses resistance.展开更多
基金supported by grants of the National Key R&D Program of China(Grants 2021YFA0909600 to X.S.and Y.Y.)National Natural Science Foundation of China(32370048 to Y.Y.,Grants 31725003 and 32330004 to X.S.).
文摘Zur(zinc uptake regulator),a member of the Fur(ferric uptake regulator)family of transcriptional regulators,plays multifaceted roles by regulating the gene expressions,such as modulating zinc ion uptake by regulating the znuABC gene cluster and influencing bacterial motility by modulating genes associated with flagella or pili.The photosynthetic autotroph Synechocystis sp.PCC 6803 is frequently used as an indicator organism for water pollution and a cell factory for high-value biochemical production in synthetic biology.During its growth,this organism often encounters various abiotic stresses,including oxidative,salt,and antibiotic stress.In this study,we conducted transcriptomic analysis on bothΔzur mutant and wild-type(WT)strains to identify potential Zur-regulated genes in Synechocystis sp.PCC 6803.These genes primarily participate in multiple pathways such as inorganic ion transport,carbohydrate transport,energy production and conversion,and cell motility.Zur not only controls zinc ion homeostasis within the cell but also influences the iron balance by directly regulating the expression of the fur gene.In terms of motility,Zur regulates the expression of bacterial pili gene cluster and other motility-related genes,thereby affecting the twitching motility of Synechocystis sp.PCC 6803.Furthermore,Zur plays a crucial role in promoting biofilm formation and enhancing resistance to salt,oxidative,and antibiotic stresses by modulating relative gene expression.In conclusion,as a global transcriptional regulator,Zur plays pivotal roles in metal ion homeostasis,motility,and resistance to multiple stresses in Synechocystis sp.PCC 6803.This study illustrates the Zur regulons in Synechocystis sp.PCC 6803,and underscores the importance of Zur in enhancing the environmental adaptability of cyanobacteria.Highlights 1.Transcriptome analysis of Zur’s regulons inSynechocystis sp.PCC 6803.2.Zur mediates metal homeostasis beyond zinc.3.Zur modulates pili biosynthesis and subsequent motility.4.Zur promotes biofilm formation and stresses resistance.
