No commercial“on-label”transfemoral transcatheter aortic valve replacement(TAVR)device for aortic regurgitation(AR)is available in most countries.TAVR for AR with an“off-label”self-expanding device has shown accep...No commercial“on-label”transfemoral transcatheter aortic valve replacement(TAVR)device for aortic regurgitation(AR)is available in most countries.TAVR for AR with an“off-label”self-expanding device has shown acceptable procedural success but it elevated rates of early mortality and other complications.Valve disposition or displacement frequently occurs during transfemoral TAVR(TF-TAVR)for AR with a self-expanding valve,because of weak an-choring force.To overcome this problem,we propose a transcatheter“sandwich”valve-in-valve implantation(SVIV)technique,which has achieved good results.Our initial results and experience in a case series comprising seven patients suggest that the SVIV technique using commercially available self-expanding valves may be an option for treating selected patients with AR with high risk for surgical valve replacement.Compared with the traditional method,this technique can decrease the incidence of complications,particularly conduction block,but several technical details re-quire the operator’s attention.Additional research is required to confirm the superiority of this technology.展开更多
Protein hydrolysates rich in bioactive peptides are associated with antihypertension.Here,we hypothesized fermented black sesame seed hydrolysate(FBSSH),which was prepared via a unique combined process of Lac-tiplanti...Protein hydrolysates rich in bioactive peptides are associated with antihypertension.Here,we hypothesized fermented black sesame seed hydrolysate(FBSSH),which was prepared via a unique combined process of Lac-tiplantibacillus plantarum NCU116 fermentation and acid protease hydrolysis,as a functional food with the po-tential to lower blood pressure.This study is the first to systematically explore the antihypertensive effects and underlying mechanisms of FBSSH on spontaneously hypertensive rats(SHRs)by integrating histopathological analysis,biochemical serum determination,and untargeted serum metabolomics.The results showed that FBSSH performed better than untreated black sesame seeds after a ten-week intervention in reducing systolic blood pressure(-23.75 mmHg vs.-11.33 mmHg).Biochemical analysis showed that FBSSH effectively improved endothelial dysfunction(increasing nitric oxide to 2.61μmol/L and decreasing endothelin-1 to 325.99 ng/L),alleviated cardiac injury(lowering cardiac troponin T to 25.47 ng/L and creatine kinase-MB to 15.36 ng/mL),and mitigated renal damage(reducing uric acid to 53.65μmol/L,blood urea nitrogen to 4.05 mM,and creatinine to 28.10μmol/L).Additionally,FBSSH enhanced antioxidant capacity,with superoxide dismutase activity increasing to 3060.64 U/L and malondialdehyde content decreasing to 4.95 nmol/mL.Particularly,the serum metabolite profile was significantly altered by FBSSH and 54 differential metabolites were identified.These metabolites may possess antihypertensive activity of FBSSH by regulating Primary bile acid biosynthesis(e.g.,upregulating taurocholate and glycocholate),Purine metabolism(e.g.,downregulating uric acid),Histidine metabolism,Glycine,serine and threonine metabolism,and so on.Collectively,FBSSH exhibited potential for hypertensive alleviation as a functional food.展开更多
This study aimed to explore the anti-tumor activity and mechanisms of the ethanol-soluble acidic components(ESAC)of Camellia oleifera cake through a combination of network pharmacology,in vitro and in vivo studies,and...This study aimed to explore the anti-tumor activity and mechanisms of the ethanol-soluble acidic components(ESAC)of Camellia oleifera cake through a combination of network pharmacology,in vitro and in vivo studies,and metabolomics based on UPLC-Q-TOF/MS.Additionally,the potential application of ESAC in combination with PD-1 inhibitors was explored.In this work,our results demonstrated that ESAC exhibited anti-tumor activities mediated by regulated cell death(RCD),which were associated with apoptosis,pyroptosis,and ferroptosis.UPLC-Q-TOF/MS-based serum metabolomics revealed that metabolic disorders were occurred in tumor group when compared to control group.In contrast,ESAC significantly inhibited abnormal energy metabolism including glycolysis,tricarboxylic acid cycle,and amino acid metabolism.Consequently,hexokinase and malate dehydrogenase,key enzymes of energy metabolism,were determined,and data showed that treatment of tumor mice with ESAC significantly suppressed activities of these metabolic enzymes when compared to tumor group,confirming that mitigating metabolic disorders contributed to the anti-tumor activities of ESAC.Moreover,ESAC could also reduced PD-L1 expression in tumor tissue,which was similar to the effect of PD-1 inhibitor.Furthermore,combination treatment with ESAC and PD-1 inhibitor was superior to either treatment alone,and it enhanced pyroptosis through the NLRP3/Caspase-1/GSDMD pathway.Together,we concluded that metabolic correction and PD-L1 inhibition were the critical mechanisms to exert anti-tumor activities of ESAC.展开更多
基金supported by the National Natural Science Foundation of China(NSFC 82270377).
文摘No commercial“on-label”transfemoral transcatheter aortic valve replacement(TAVR)device for aortic regurgitation(AR)is available in most countries.TAVR for AR with an“off-label”self-expanding device has shown acceptable procedural success but it elevated rates of early mortality and other complications.Valve disposition or displacement frequently occurs during transfemoral TAVR(TF-TAVR)for AR with a self-expanding valve,because of weak an-choring force.To overcome this problem,we propose a transcatheter“sandwich”valve-in-valve implantation(SVIV)technique,which has achieved good results.Our initial results and experience in a case series comprising seven patients suggest that the SVIV technique using commercially available self-expanding valves may be an option for treating selected patients with AR with high risk for surgical valve replacement.Compared with the traditional method,this technique can decrease the incidence of complications,particularly conduction block,but several technical details re-quire the operator’s attention.Additional research is required to confirm the superiority of this technology.
基金supported by the National Natural Science Foundation of China(Project No.32260575)Natural Science Fund Project of Jiangxi Province(Project No.20224ACB218007)+5 种基金Young Scholar Project of the First Affiliated Hospital of Nanchang University(Project No.YFYPY202543)Key Project of Jiangxi Province’s Key Research and Development Program(Project No.20243BCC31004)National Key Laboratory Project(Project No.20232BCD44005)Jiangxi Province Key Research and Development Program(Project No.20232BBF60024)The Position Expert Project of Jiangxi Province Vegetable Industry Technology System(Project No.JXARS-04)Hainan Province Key Research and Development Project(Project No.ZDYF2024XDNY277).
文摘Protein hydrolysates rich in bioactive peptides are associated with antihypertension.Here,we hypothesized fermented black sesame seed hydrolysate(FBSSH),which was prepared via a unique combined process of Lac-tiplantibacillus plantarum NCU116 fermentation and acid protease hydrolysis,as a functional food with the po-tential to lower blood pressure.This study is the first to systematically explore the antihypertensive effects and underlying mechanisms of FBSSH on spontaneously hypertensive rats(SHRs)by integrating histopathological analysis,biochemical serum determination,and untargeted serum metabolomics.The results showed that FBSSH performed better than untreated black sesame seeds after a ten-week intervention in reducing systolic blood pressure(-23.75 mmHg vs.-11.33 mmHg).Biochemical analysis showed that FBSSH effectively improved endothelial dysfunction(increasing nitric oxide to 2.61μmol/L and decreasing endothelin-1 to 325.99 ng/L),alleviated cardiac injury(lowering cardiac troponin T to 25.47 ng/L and creatine kinase-MB to 15.36 ng/mL),and mitigated renal damage(reducing uric acid to 53.65μmol/L,blood urea nitrogen to 4.05 mM,and creatinine to 28.10μmol/L).Additionally,FBSSH enhanced antioxidant capacity,with superoxide dismutase activity increasing to 3060.64 U/L and malondialdehyde content decreasing to 4.95 nmol/mL.Particularly,the serum metabolite profile was significantly altered by FBSSH and 54 differential metabolites were identified.These metabolites may possess antihypertensive activity of FBSSH by regulating Primary bile acid biosynthesis(e.g.,upregulating taurocholate and glycocholate),Purine metabolism(e.g.,downregulating uric acid),Histidine metabolism,Glycine,serine and threonine metabolism,and so on.Collectively,FBSSH exhibited potential for hypertensive alleviation as a functional food.
基金supported by the Health Care Commission of Jiangxi Province(No:202410327)Chinese medicine administration of Jiangxi province(No:2023B1301)+1 种基金Special Financial Science and Technology Project of Jiangxi province(No:ZBG20230418013)Nanchang University Graduate Innovation Special Fund Project(YC2023-S057 and YC2023-S173).
文摘This study aimed to explore the anti-tumor activity and mechanisms of the ethanol-soluble acidic components(ESAC)of Camellia oleifera cake through a combination of network pharmacology,in vitro and in vivo studies,and metabolomics based on UPLC-Q-TOF/MS.Additionally,the potential application of ESAC in combination with PD-1 inhibitors was explored.In this work,our results demonstrated that ESAC exhibited anti-tumor activities mediated by regulated cell death(RCD),which were associated with apoptosis,pyroptosis,and ferroptosis.UPLC-Q-TOF/MS-based serum metabolomics revealed that metabolic disorders were occurred in tumor group when compared to control group.In contrast,ESAC significantly inhibited abnormal energy metabolism including glycolysis,tricarboxylic acid cycle,and amino acid metabolism.Consequently,hexokinase and malate dehydrogenase,key enzymes of energy metabolism,were determined,and data showed that treatment of tumor mice with ESAC significantly suppressed activities of these metabolic enzymes when compared to tumor group,confirming that mitigating metabolic disorders contributed to the anti-tumor activities of ESAC.Moreover,ESAC could also reduced PD-L1 expression in tumor tissue,which was similar to the effect of PD-1 inhibitor.Furthermore,combination treatment with ESAC and PD-1 inhibitor was superior to either treatment alone,and it enhanced pyroptosis through the NLRP3/Caspase-1/GSDMD pathway.Together,we concluded that metabolic correction and PD-L1 inhibition were the critical mechanisms to exert anti-tumor activities of ESAC.
