With brick-wall solar greenhouses in Changli area as the research object,using temperature dynamic monitoring and statistical methods,the greenhouse structure suitable for promoting early cultivation of local peach tr...With brick-wall solar greenhouses in Changli area as the research object,using temperature dynamic monitoring and statistical methods,the greenhouse structure suitable for promoting early cultivation of local peach trees was selected by studying the temperature data of the solar greenhouses during the winter solstice,and a prediction model for daily average temperature was constructed.The results showed that greenhouse Ⅰ had reasonable structural parameters and good daylight during the day.However,due to the low wall thickness and poor insulation material,the minimum temperature was significantly lower than other greenhouses.The thermal insulation performance of greenhouse Ⅱ and Ⅲ was better than that of greenhouse Ⅰ,but the depth-span ratio and the front roof lighting angle were smaller.During the winter solstice,the average temperature of the three greenhouses was between 10 and 15℃,which was suitable for early cultivation of peach trees.The prediction model of daily average temperature was obtained:Daily average temperature=1.02+0.69×Daily average temperature of the previous day+0.02×Maximum temperature of the previous day-0.01×Minimum temperature of the previous day.To sum up,the structural parameters of brick-wall solar greenhouses suitable for early cultivation of peach trees in Changli area were as follows:span 6.5-8.5 m,depth-span ratio 0.47,front roof lighting angle 30°and wall thickness greater than 55 cm.展开更多
Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and ac...Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and activating blood,awakening the brain,relieving cough and regulating menstruation.With the development of modern medicine,the active components and therapeutic mechanisms of L.capillipes Hemsl have been gradually revealed.The present report systematically reviews the chemical composition and biological activities of L.capillipes Hemsl,to provide scientific basis and reference for detailed research on L.capillipes Hemsl.展开更多
Pectobacterium carotovorum(Pc)is a necrotrophic bacterial pathogen that causes soft rot disease in Brassica rapa crops,including Chinese cabbage(B.rapa ssp.pekinensis),resulting in significant yield losses worldwide(R...Pectobacterium carotovorum(Pc)is a necrotrophic bacterial pathogen that causes soft rot disease in Brassica rapa crops,including Chinese cabbage(B.rapa ssp.pekinensis),resulting in significant yield losses worldwide(Roh et al.,2010).Deployment of resistant Chinese cabbage varieties and mining novel resistance genes for breeding resistant varieties against soft rot are key approaches that are currently used to prevent soft rot infections and ensure high yield and quality in Chinese cabbage.However,the highly adapted pathogen can easily overcome resistance(R)gene-mediated defense responses.Thus,discovering novel resistance genes and understanding the molecular mechanisms underlying R-mediated resistance against soft rot are sustained strategies for Chinese cabbage breeding.展开更多
In recent years,diabetes has posed a serious threat to human health.Traditional Chinese medicine for lowering blood sugar has attracted much attention due to its unique advantages in the treatment of diabetes.This pap...In recent years,diabetes has posed a serious threat to human health.Traditional Chinese medicine for lowering blood sugar has attracted much attention due to its unique advantages in the treatment of diabetes.This paper summarizes the research results on common traditional Chinese medicines for lowering blood sugar in recent years.These results lay a theoretical foundation for the future clinical development and use of hypoglycemic Chinese medicine,the improvement of the quality standards of hypoglycemic Chinese medicine,and the in-depth research on the mechanisms.展开更多
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm...Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.展开更多
Balancing electron transfer and intermediate adsorption ability of bifunctional catalysts via tailoring electronic structures is crucial for green hydrogen production,while it still remains challenging due to lacking ...Balancing electron transfer and intermediate adsorption ability of bifunctional catalysts via tailoring electronic structures is crucial for green hydrogen production,while it still remains challenging due to lacking efficient strategies.Herein,one efficient and universal strategy is developed to greatly regulate electronic structures of the metallic Ni-Fe-P catalysts via in-situ introducing the rare earth(RE)atoms(Ni-Fe-RE-P,RE=La,Ce,Pr,and Nd).Accordingly,the as-prepared optimal Ni-Fe-Ce-P/CC self-supported bifunctional electrodes exhibited superior electrocatalytic activity and excellent stability with the low overpotentials of 247 and 331 mV at 100 mA cm^(-2) for HER and OER,respectively.In the assembled electrolyzer,the Ni-Fe-Ce-P/CC as bifunctional electrodes displayed low operation potential of 1.49 V to achieve a current density of 10 mA cm^(-2),and the catalytic performance can be maintained for 100 h.Experimental results combined with density functional theory(DFT)calculation reveal that Ce doping leads to electron decentralization and crystal structure distortion,which can tailor the band structures and d-band center of Ni-Fe-P,further increasing conductivity and optimizing intermediate adsorption energy.Our work not only proposes a valuable strategy to regulate the electron transfer and intermediate adsorption of electrocatalysts via RE atoms doping,but also provides a deep under-standing of regulation mechanism of metallic electrocatalysts for enhanced water splitting.展开更多
To investigate the dissolution behaviors of Al_(2)O_(3)inclusions in CaO-5wt%MgO-SiO_(2)-30wt%Al_(2)O_(3)-TiO_(2)system ladle slags,confocal scanning laser microscopy was conducted on the slags with different TiO_(2)c...To investigate the dissolution behaviors of Al_(2)O_(3)inclusions in CaO-5wt%MgO-SiO_(2)-30wt%Al_(2)O_(3)-TiO_(2)system ladle slags,confocal scanning laser microscopy was conducted on the slags with different TiO_(2)contents(0-10wt%),and scanning electron microscopy was performed to study the interfacial reaction between Al_(2)O_(3)and this slag system.The results disclose that the dissolution of Al_(2)O_(3)inclusions does not result in the formation of new phases at the boundary between the slag and the inclusions.In TiO_(2)-bearing and TiO_(2)-free ladle slags,there is no difference in the dissolution mechanism of Al_(2)O_(3)inclusions at steelmaking temperatures.Boundary layer diffusion is found as the controlling step of the dissolution of Al_(2)O_(3),and the diffusion coefficient is in the range of 4.18×10^(-10)to 2.18×10^(-9)m^(2)/s at 1450-1500℃.Compared with the solubility of Al_(2)O_(3)in the slags,slag viscosity and temperature play a more profound role in the dissolution of Al_(2)O_(3)inclusions.A lower viscosity and a lower melting point of the slags are beneficial for the dissolution.Suitable addition of TiO_(2)(e.g.,5wt%)in ladle slags can enhance the dissolution of Al_(2)O_(3)inclusions because of the low viscosity and melting point of the slags,while excessive addition of TiO_(2)(e.g.,10wt%)shows the opposite trend.展开更多
Liquid metal(LM)and liquid metal alloys(LMs)possess unique physicochemical features,which have become emerging and functionalized materials that are attractive applicants in various fields.Herein,uniform LM nanodrople...Liquid metal(LM)and liquid metal alloys(LMs)possess unique physicochemical features,which have become emerging and functionalized materials that are attractive applicants in various fields.Herein,uniform LM nanodroplets armored by carbon dots(LMD@CDs)were prepared and exhibited high colloidal stability in various solvents,as well as water.After optimization,LMD@CDs can be applied as functional additives for the 3D/4D printing of hydrogel and cross-linked resin through digital light processing(DLP).The light absorption of LMD@CDs not only improved the printing accuracy,but also led to the cross-linking density differential during the post-curing process.Base on the cross-linking density differential of soft hydrogel and photothermal performance of the LM,the 3D printed objects can exhibit stimulus responses to both water and laser irradiation.Additionally,the CDs shell and LM core of LMD@CDs provide the printed objects interesting photoluminescence and electric conductivity capabilities,respectively.We deduce this versatile 3D/4D printing system would provide a new platform for the preparation of multi-functional and stimuli-responsive advance materials.展开更多
Croton crassifolius (C. crassifolius) is a kind of traditional Chinese medicine, with a history hundreds of years in China. By consulting Sciencedirect databases and Web of Science databases, 68 related articles were ...Croton crassifolius (C. crassifolius) is a kind of traditional Chinese medicine, with a history hundreds of years in China. By consulting Sciencedirect databases and Web of Science databases, 68 related articles were found, 12 of which were related to chemical composition and pharmacological activity. It was used by the ancients as the medicine to treat stomach trouble, rheumatism, poliomyelitis and injuries, and it is the treasure of traditional Chinese medicine in China. With the development of modern medicine, the active ingredients and therapeutic mechanism of C. crassifolius have been gradually revealed. In this study, the functions and applications of C. crassifolius were reviewed from the aspects of botany, traditional Chinese medicine therapy and biotransformation of substances, so as to better understand and use C. crassifolius and provide more effective drug choices for clinical treatment.展开更多
背景和目的环状RNA(circular RNAs,circRNAs)在包括肿瘤发生在内的多种病理过程中发挥着至关重要的作用。目前,在癌症研究中报道最多的circRNA为外显子circRNA,内含子circRNA的研究相对较少。本文旨在探索内含子circRNA在头颈部鳞状细胞...背景和目的环状RNA(circular RNAs,circRNAs)在包括肿瘤发生在内的多种病理过程中发挥着至关重要的作用。目前,在癌症研究中报道最多的circRNA为外显子circRNA,内含子circRNA的研究相对较少。本文旨在探索内含子circRNA在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的调控作用。方法我们首先对4对HNSCC患者的原发肿瘤组织和癌旁组织其进行了全转录组测序分析。然后分析了circGNG7在HNSCC组织和细胞系中的表达特征,并探讨其与HNSCC患者预后的关系。我们同时鉴定了与circGNG7相互作用的功能蛋白,这些相互作用通过改变下游信号通路进而调控HNSCC的进展。结果在本项研究中,我们发现了一种新的内含子circRNA——circGNG7,并验证了其在HNSCC进展中的功能作用。CircGNG7主要定位于细胞质,其在HNSCC组织和细胞系(CAL27、CAL33、SCC4、SCC9、HN6和HN30)中的表达量均下调。HNSCC患者中circGNG7低表达与不良预后显著相关。过表达circGNG7可显著抑制肿瘤细胞增殖、克隆集落形成、体外迁移和体内肿瘤的生长能力。在机制上,HNSCC细胞中circGNG7的表达受到转录因子SMAD家族成员4(SMAD family member 4,SAMD4)的调控。circGNG27可与功能性热休克蛋白27(heat shock protein 27,HSP27)的第78和82位丝氨酸残基结合,占据其磷酸化位点并阻碍其磷酸化,从而抑制HSP27-JNK/P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)致癌信号通路。在HNSCC中下调circGNG7的表达可激活HSP27-JNK/P38 MAPK信号通路,进而促进肿瘤的发展。结论我们的研究表明,circGNG7是一种新的内含子circRNA,具有较强的肿瘤抑制功能,同时发现circGNG7/HSP27-JNK/P38 MAPK信号通路是一种新的抑制HNSCC发展的分子机制。展开更多
背景和目的越来越多的证据表明,环状RNA(circular RNAs,circRNAs)在肿瘤发生等病理过程中发挥着至关重要的作用。在癌症研究中研究最多的circRNA是外显子circRNA,而内含子circRNA的研究远不及外显子circRNA。本文旨在探索内含子circRNA...背景和目的越来越多的证据表明,环状RNA(circular RNAs,circRNAs)在肿瘤发生等病理过程中发挥着至关重要的作用。在癌症研究中研究最多的circRNA是外显子circRNA,而内含子circRNA的研究远不及外显子circRNA。本文旨在探索内含子circRNA在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的调控作用。方法我们对4例HNSCC患者的原发癌组织及其配对癌旁组织进行全转录组测序分析,分析circGNG7在HNSCC组织和细胞系中的表达特征,并探讨其与HNSCC患者预后的关系。鉴定与circGNG7相互作用的功能蛋白,研究它们相互作用改变下游信号通路,进而调控HNSCC进展的分子机制。结果我们发现了一种新的内含子circRNA——circGNG7,并分析和验证了其在HNSCC进展中的作用。CircGNG7主要定位于细胞质,在HNSCC组织和细胞系(CAL27、CAL33、SCC4、SCC9、HN6和HN30)中表达均下调。在HNSCC患者中,circGNG7低表达与不良预后显著相关。circGNG7过表达可显著抑制肿瘤细胞增殖、克隆形成、体外迁移和体内肿瘤生长能力。在机制方面,HNSCC细胞中circGNG7的表达受到转录因子SMAD家族成员4(SMAD family member 4,SAMD4)的调控。circGNG27可与功能性热休克蛋白27(heat shock protein 27,HSP27)的第78和82位丝氨酸残基结合,占据其磷酸化位点并阻碍其磷酸化,从而抑制HSP27-JNK/P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)致癌信号通路。在HNSCC中下调circGNG7表达,可激活HSP27-JNK/P38 MAPK信号通路,进而促进肿瘤进展。结论本研究表明,circGNG7是一种新的内含子circRNA,其具有较强的肿瘤抑制功能,通过circGNG7/HSP27-JNK/P38 MAPK信号通路抑制HNSCC进展。展开更多
Through this study,we aim to construct a teaching model for basic medical courses that enhances“clinical competency.”We explore the optimal methods and pathways for the organic integration of exam-oriented teaching ...Through this study,we aim to construct a teaching model for basic medical courses that enhances“clinical competency.”We explore the optimal methods and pathways for the organic integration of exam-oriented teaching and professional teaching,optimize teaching efficiency,improve teaching quality and educational effectiveness,and provide theoretical support for the cultivation of clinical medical talents in China.The goal is to establish a talent cultivation objective oriented towards“clinical competency,”promote the teaching reform of basic medical courses in comprehensive universities,facilitate the transition of basic medical course teaching from“teacher knowledge presentation”to“student knowledge construction,”and cultivate high-quality medical talents with clinical competency.展开更多
短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研...短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研究使用Slooten与Ricciardi提出的有限突变模型和大规模数据,对28,313例(78,739个体)中国北京汉族已确认亲生关系的亲子鉴定案的20个常染色体STR基因座(D3S1358、D1S1656、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D6S1043、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433和FGA;由于有限突变模型中未包含D6S1043的矫正参数,因此本文实际计算其余19个STR基因座的突变率)进行了调查。结果发现,所有基因座均存在突变现象,总计发生1665个突变事件,包括1614个一步突变,34个两步突变,8个三步突变和9个非整步突变。基因座特异性的平均实际突变率在三联体中为0.00007700(TPOX)~0.00459050(FGA),在二联体中为0.00000000(TPOX)~0.00344850(FGA)。此外,本研究还分析了表面和实际突变率、三联体和二联体突变率、父源和母源的突变率之间的关系。研究表明,实际突变率多大于表面突变率,而且μ1^(*)/μ2^(*)(表面突变率)的比值通常也大于μ1/μ2(实际突变率)(μ1^(*),μ1;μ2^(*),μ2分别是一步和两步的突变率),即更多的“隐性”突变被释放出来。而且父源和母源的三联体和二联体的突变率也有存在差异。随后,将这些突变率数据与已发表的中国其他汉族人口的相关研究进行比较,展现出了STR突变率的时间与区域差异。由于样本量大,本研究中还报告了一些少见的突变事件,例如同卵双胞胎突变和“假四步突变”等。综上所述,本研究通过大量数据获得了接近真实的STR突变率的估计值,不仅可为中国法医DNA数据库和群体遗传学数据库提供重要的基础数据,也对开展法医学个体识别、亲权鉴定和遗传学研究具有重要的意义。展开更多
Large amounts of cyanide tailings are produced during the cyanidation process in gold extraction,which are hazardous solid wastes due to the toxic cyanide.Pyrite is one of the main minerals in cyanide tailings.The rem...Large amounts of cyanide tailings are produced during the cyanidation process in gold extraction,which are hazardous solid wastes due to the toxic cyanide.Pyrite is one of the main minerals in cyanide tailings.The removal of cyanide adsorbed on pyrite by H_2O_2 oxidation under alkaline conditions was investigated in this study.It was found that the removal efficiency was positively correlated with pH from 5 to 12,but remained almost constant when pH was higher than 12.The highest cyanide removal efficiency of 91.10% was achieved by adding no less than 0.6 wt.% of H_2O_2.Cyanide removal was positively correlated with the CN^-adsorption amount between 1.06 and 8.5 mg/g,and temperature between 25 and 85°C.The removal of cyanide adsorbed on pyrite by H_2O_2 oxidation under alkaline conditions was due to the oxidation of pyrite.Hexacyanoferrate,thiocyanate and sulfate were generated with mole ratios of about 2.03:1.12:3.17 during the cyanide removal.?2018 The Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences.展开更多
OBJECTIVE： To evaluate systematically the clinical efficacy and safety of Qingkailing （QKL） injection in the treatment of acute stroke. METHODS： Searches for randomized controlled trials into acute stroke treated ...OBJECTIVE： To evaluate systematically the clinical efficacy and safety of Qingkailing （QKL） injection in the treatment of acute stroke. METHODS： Searches for randomized controlled trials into acute stroke treated with QKL injection were performed in the China National Knowledge Infrastructure Database, China Science and Technology Journal Database, Wan fang Database, Chinese Biomedical Literature Database, PubMed and Cochrane Library, from January 1979 to March 2013. Two reviewers independently retrieved the RCTs and extracted the information. The Cochrane risk of bias method was used to assess the quality of the included studies, and a Meta-analysis was conducted with Review Manager 5.2 software. RESULTS： A total of 13 studies with 1110 participants were included. The quality of the studies was generally low. The Meta-analysis indicated that the combined use of QKL and Western Medicine was significantly superior to control group therapy in terms of the total effective rate. The relative risk （RR） in the acute cerebral hemorrhage （ACH） sub-group was 1.17 [95% confidence interval （CI） （1.08, 1.26）, P=0.0001]. In the acute cerebral infarction （ACI） sub-group, RR was 1.27 [9.5% CI （1.14, 1.42）, P〈0.0001], and in the ACH and ACI mixed sub-group, RR was 1.34 [95% CI （1.20,1.50）,P〈0.00001]. Additionally, QKL promoted the absorption of hematoma [mean difference （MD）= - 3.73, 95%0 （ - 4.48, - 2.98）, P〈0.000 01], decreased neurological damage in ACI [MD= - 5.60, 95% CI （ - 8.50, - 2.70）, P=0.0002] and ACH [MD= 4.08, 95% CI （ - 8.00, 0.16）, P=0.04], promoted the recovery of awareness [RR=1.56, 95% CI （1.09, 2.21）, P=0.01] and reduced the whole blood viscosity coefficient [MD=- 0.75, 95% CI （ - 1.47, - 0.03）, P=0.04]. There were no adverse drug reactions reported in the included studies. CONCLUSION＂ Based on this systematic review, QKL combined with conventional therapy was effective compared with control treatment. However, because the articles used in the study were not of high quality, further studies should be conducted into the efficacy and safety of QKL in treating acute stroke.展开更多
基金Supported by Modern Agricultural Industry Technology System Innovation Team Construction in Hebei Province(HBCT2023130404).
文摘With brick-wall solar greenhouses in Changli area as the research object,using temperature dynamic monitoring and statistical methods,the greenhouse structure suitable for promoting early cultivation of local peach trees was selected by studying the temperature data of the solar greenhouses during the winter solstice,and a prediction model for daily average temperature was constructed.The results showed that greenhouse Ⅰ had reasonable structural parameters and good daylight during the day.However,due to the low wall thickness and poor insulation material,the minimum temperature was significantly lower than other greenhouses.The thermal insulation performance of greenhouse Ⅱ and Ⅲ was better than that of greenhouse Ⅰ,but the depth-span ratio and the front roof lighting angle were smaller.During the winter solstice,the average temperature of the three greenhouses was between 10 and 15℃,which was suitable for early cultivation of peach trees.The prediction model of daily average temperature was obtained:Daily average temperature=1.02+0.69×Daily average temperature of the previous day+0.02×Maximum temperature of the previous day-0.01×Minimum temperature of the previous day.To sum up,the structural parameters of brick-wall solar greenhouses suitable for early cultivation of peach trees in Changli area were as follows:span 6.5-8.5 m,depth-span ratio 0.47,front roof lighting angle 30°and wall thickness greater than 55 cm.
基金supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and activating blood,awakening the brain,relieving cough and regulating menstruation.With the development of modern medicine,the active components and therapeutic mechanisms of L.capillipes Hemsl have been gradually revealed.The present report systematically reviews the chemical composition and biological activities of L.capillipes Hemsl,to provide scientific basis and reference for detailed research on L.capillipes Hemsl.
基金supported by the Natural Science Foundation of Hebei(Grant Nos.C2022204116 and C2024204246)Hundred Talents Program for the introduction of high-level overseas talents in Hebei Province(Grant No.E2020100004)+3 种基金the Key Research and Development Program of Hebei(Grant No.21326311D-2)the Basic Research Project of Hebei Universities in Shijiazhuang(Grant No.241791217A)the Science Research Project of Hebei Education Department(Grant No.BJK2024079)S&T Program of Hebei(Grant No.246Z6302G)。
文摘Pectobacterium carotovorum(Pc)is a necrotrophic bacterial pathogen that causes soft rot disease in Brassica rapa crops,including Chinese cabbage(B.rapa ssp.pekinensis),resulting in significant yield losses worldwide(Roh et al.,2010).Deployment of resistant Chinese cabbage varieties and mining novel resistance genes for breeding resistant varieties against soft rot are key approaches that are currently used to prevent soft rot infections and ensure high yield and quality in Chinese cabbage.However,the highly adapted pathogen can easily overcome resistance(R)gene-mediated defense responses.Thus,discovering novel resistance genes and understanding the molecular mechanisms underlying R-mediated resistance against soft rot are sustained strategies for Chinese cabbage breeding.
文摘In recent years,diabetes has posed a serious threat to human health.Traditional Chinese medicine for lowering blood sugar has attracted much attention due to its unique advantages in the treatment of diabetes.This paper summarizes the research results on common traditional Chinese medicines for lowering blood sugar in recent years.These results lay a theoretical foundation for the future clinical development and use of hypoglycemic Chinese medicine,the improvement of the quality standards of hypoglycemic Chinese medicine,and the in-depth research on the mechanisms.
基金financially supported by the Natural Science Foundation of Hebei Province-Innovation Group Research Project(Grant No.C2020204111)the National Natural Science Foundation of China(Grant No.31930098)+3 种基金the Science Fund for Distinguished Young Scholars of Hebei Province(Grant No.C2021204049)the Hebei Province Outstanding Youth Fund(Grant No.BJ2021024)the Hebei Provincial Key Research Projects(21326344D)Hebei International Joint Research Base of Modern Agricultural Biotechnology.
文摘Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.
基金support from the National Key Technology R&D Program of China(2021YFB3500801,2022YFC3901503,2022YFB3504302)the Natural Science Foundation and Overseas Talent Projects of Jiangxi Province(20232BAB214025,20232BCJ25044).
文摘Balancing electron transfer and intermediate adsorption ability of bifunctional catalysts via tailoring electronic structures is crucial for green hydrogen production,while it still remains challenging due to lacking efficient strategies.Herein,one efficient and universal strategy is developed to greatly regulate electronic structures of the metallic Ni-Fe-P catalysts via in-situ introducing the rare earth(RE)atoms(Ni-Fe-RE-P,RE=La,Ce,Pr,and Nd).Accordingly,the as-prepared optimal Ni-Fe-Ce-P/CC self-supported bifunctional electrodes exhibited superior electrocatalytic activity and excellent stability with the low overpotentials of 247 and 331 mV at 100 mA cm^(-2) for HER and OER,respectively.In the assembled electrolyzer,the Ni-Fe-Ce-P/CC as bifunctional electrodes displayed low operation potential of 1.49 V to achieve a current density of 10 mA cm^(-2),and the catalytic performance can be maintained for 100 h.Experimental results combined with density functional theory(DFT)calculation reveal that Ce doping leads to electron decentralization and crystal structure distortion,which can tailor the band structures and d-band center of Ni-Fe-P,further increasing conductivity and optimizing intermediate adsorption energy.Our work not only proposes a valuable strategy to regulate the electron transfer and intermediate adsorption of electrocatalysts via RE atoms doping,but also provides a deep under-standing of regulation mechanism of metallic electrocatalysts for enhanced water splitting.
基金financially supported by the National Natural Science Foundation of China(Nos.U20A20272 and52074073)the Fundamental Research Funds for the Central Universities(No.2325035)。
文摘To investigate the dissolution behaviors of Al_(2)O_(3)inclusions in CaO-5wt%MgO-SiO_(2)-30wt%Al_(2)O_(3)-TiO_(2)system ladle slags,confocal scanning laser microscopy was conducted on the slags with different TiO_(2)contents(0-10wt%),and scanning electron microscopy was performed to study the interfacial reaction between Al_(2)O_(3)and this slag system.The results disclose that the dissolution of Al_(2)O_(3)inclusions does not result in the formation of new phases at the boundary between the slag and the inclusions.In TiO_(2)-bearing and TiO_(2)-free ladle slags,there is no difference in the dissolution mechanism of Al_(2)O_(3)inclusions at steelmaking temperatures.Boundary layer diffusion is found as the controlling step of the dissolution of Al_(2)O_(3),and the diffusion coefficient is in the range of 4.18×10^(-10)to 2.18×10^(-9)m^(2)/s at 1450-1500℃.Compared with the solubility of Al_(2)O_(3)in the slags,slag viscosity and temperature play a more profound role in the dissolution of Al_(2)O_(3)inclusions.A lower viscosity and a lower melting point of the slags are beneficial for the dissolution.Suitable addition of TiO_(2)(e.g.,5wt%)in ladle slags can enhance the dissolution of Al_(2)O_(3)inclusions because of the low viscosity and melting point of the slags,while excessive addition of TiO_(2)(e.g.,10wt%)shows the opposite trend.
基金The financial support from the National Natural Science Foundation of China(Grant No.51973201,U1804128,to X.P.Grant No.52173209,to Y.H.)+4 种基金the 111 Project(D18023,to X.P.)the National Science Foundation for Young Scientists of China(Grant No.22105179,to G.S.)the National Key R&D Program of China(2017YFB0307600,to M.L)China Postdoctoral Science Foundation(2020M682317,to X.Z)Scientific&technological research projects in Henan Province(222102520009,to X.Q.)
文摘Liquid metal(LM)and liquid metal alloys(LMs)possess unique physicochemical features,which have become emerging and functionalized materials that are attractive applicants in various fields.Herein,uniform LM nanodroplets armored by carbon dots(LMD@CDs)were prepared and exhibited high colloidal stability in various solvents,as well as water.After optimization,LMD@CDs can be applied as functional additives for the 3D/4D printing of hydrogel and cross-linked resin through digital light processing(DLP).The light absorption of LMD@CDs not only improved the printing accuracy,but also led to the cross-linking density differential during the post-curing process.Base on the cross-linking density differential of soft hydrogel and photothermal performance of the LM,the 3D printed objects can exhibit stimulus responses to both water and laser irradiation.Additionally,the CDs shell and LM core of LMD@CDs provide the printed objects interesting photoluminescence and electric conductivity capabilities,respectively.We deduce this versatile 3D/4D printing system would provide a new platform for the preparation of multi-functional and stimuli-responsive advance materials.
基金supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Croton crassifolius (C. crassifolius) is a kind of traditional Chinese medicine, with a history hundreds of years in China. By consulting Sciencedirect databases and Web of Science databases, 68 related articles were found, 12 of which were related to chemical composition and pharmacological activity. It was used by the ancients as the medicine to treat stomach trouble, rheumatism, poliomyelitis and injuries, and it is the treasure of traditional Chinese medicine in China. With the development of modern medicine, the active ingredients and therapeutic mechanism of C. crassifolius have been gradually revealed. In this study, the functions and applications of C. crassifolius were reviewed from the aspects of botany, traditional Chinese medicine therapy and biotransformation of substances, so as to better understand and use C. crassifolius and provide more effective drug choices for clinical treatment.
文摘背景和目的环状RNA(circular RNAs,circRNAs)在包括肿瘤发生在内的多种病理过程中发挥着至关重要的作用。目前,在癌症研究中报道最多的circRNA为外显子circRNA,内含子circRNA的研究相对较少。本文旨在探索内含子circRNA在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的调控作用。方法我们首先对4对HNSCC患者的原发肿瘤组织和癌旁组织其进行了全转录组测序分析。然后分析了circGNG7在HNSCC组织和细胞系中的表达特征,并探讨其与HNSCC患者预后的关系。我们同时鉴定了与circGNG7相互作用的功能蛋白,这些相互作用通过改变下游信号通路进而调控HNSCC的进展。结果在本项研究中,我们发现了一种新的内含子circRNA——circGNG7,并验证了其在HNSCC进展中的功能作用。CircGNG7主要定位于细胞质,其在HNSCC组织和细胞系(CAL27、CAL33、SCC4、SCC9、HN6和HN30)中的表达量均下调。HNSCC患者中circGNG7低表达与不良预后显著相关。过表达circGNG7可显著抑制肿瘤细胞增殖、克隆集落形成、体外迁移和体内肿瘤的生长能力。在机制上,HNSCC细胞中circGNG7的表达受到转录因子SMAD家族成员4(SMAD family member 4,SAMD4)的调控。circGNG27可与功能性热休克蛋白27(heat shock protein 27,HSP27)的第78和82位丝氨酸残基结合,占据其磷酸化位点并阻碍其磷酸化,从而抑制HSP27-JNK/P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)致癌信号通路。在HNSCC中下调circGNG7的表达可激活HSP27-JNK/P38 MAPK信号通路,进而促进肿瘤的发展。结论我们的研究表明,circGNG7是一种新的内含子circRNA,具有较强的肿瘤抑制功能,同时发现circGNG7/HSP27-JNK/P38 MAPK信号通路是一种新的抑制HNSCC发展的分子机制。
文摘背景和目的越来越多的证据表明,环状RNA(circular RNAs,circRNAs)在肿瘤发生等病理过程中发挥着至关重要的作用。在癌症研究中研究最多的circRNA是外显子circRNA,而内含子circRNA的研究远不及外显子circRNA。本文旨在探索内含子circRNA在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的调控作用。方法我们对4例HNSCC患者的原发癌组织及其配对癌旁组织进行全转录组测序分析,分析circGNG7在HNSCC组织和细胞系中的表达特征,并探讨其与HNSCC患者预后的关系。鉴定与circGNG7相互作用的功能蛋白,研究它们相互作用改变下游信号通路,进而调控HNSCC进展的分子机制。结果我们发现了一种新的内含子circRNA——circGNG7,并分析和验证了其在HNSCC进展中的作用。CircGNG7主要定位于细胞质,在HNSCC组织和细胞系(CAL27、CAL33、SCC4、SCC9、HN6和HN30)中表达均下调。在HNSCC患者中,circGNG7低表达与不良预后显著相关。circGNG7过表达可显著抑制肿瘤细胞增殖、克隆形成、体外迁移和体内肿瘤生长能力。在机制方面,HNSCC细胞中circGNG7的表达受到转录因子SMAD家族成员4(SMAD family member 4,SAMD4)的调控。circGNG27可与功能性热休克蛋白27(heat shock protein 27,HSP27)的第78和82位丝氨酸残基结合,占据其磷酸化位点并阻碍其磷酸化,从而抑制HSP27-JNK/P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)致癌信号通路。在HNSCC中下调circGNG7表达,可激活HSP27-JNK/P38 MAPK信号通路,进而促进肿瘤进展。结论本研究表明,circGNG7是一种新的内含子circRNA,其具有较强的肿瘤抑制功能,通过circGNG7/HSP27-JNK/P38 MAPK信号通路抑制HNSCC进展。
基金Key Project of the“14th Five-Year”Plan for Heilongjiang Provincial Education Science in 2021(GJB1421168)Key Project of the“14th Five-Year”Plan for Heilongjiang Provincial Education Science in 2022(GJB1422698)+4 种基金Key Project of the“14th Five-Year”Plan for Heilongjiang Provincial Education Science in 2023(GJB1423165)Research Topic on Higher Education of Heilongjiang Provincial Association of Higher Education(23GJYBC057)Special Project for New Medical Education in Jiamusi University(2023XYK-20)“Dongji”Academic Team of Jiamusi University(DJXSTD202405)Heilongjiang Province Postgraduate Quality Course High-Quality Construction Project(HLJYJSZLTSGC-YJSJPKC-2022-082)。
文摘Through this study,we aim to construct a teaching model for basic medical courses that enhances“clinical competency.”We explore the optimal methods and pathways for the organic integration of exam-oriented teaching and professional teaching,optimize teaching efficiency,improve teaching quality and educational effectiveness,and provide theoretical support for the cultivation of clinical medical talents in China.The goal is to establish a talent cultivation objective oriented towards“clinical competency,”promote the teaching reform of basic medical courses in comprehensive universities,facilitate the transition of basic medical course teaching from“teacher knowledge presentation”to“student knowledge construction,”and cultivate high-quality medical talents with clinical competency.
文摘短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研究使用Slooten与Ricciardi提出的有限突变模型和大规模数据,对28,313例(78,739个体)中国北京汉族已确认亲生关系的亲子鉴定案的20个常染色体STR基因座(D3S1358、D1S1656、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D6S1043、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433和FGA;由于有限突变模型中未包含D6S1043的矫正参数,因此本文实际计算其余19个STR基因座的突变率)进行了调查。结果发现,所有基因座均存在突变现象,总计发生1665个突变事件,包括1614个一步突变,34个两步突变,8个三步突变和9个非整步突变。基因座特异性的平均实际突变率在三联体中为0.00007700(TPOX)~0.00459050(FGA),在二联体中为0.00000000(TPOX)~0.00344850(FGA)。此外,本研究还分析了表面和实际突变率、三联体和二联体突变率、父源和母源的突变率之间的关系。研究表明,实际突变率多大于表面突变率,而且μ1^(*)/μ2^(*)(表面突变率)的比值通常也大于μ1/μ2(实际突变率)(μ1^(*),μ1;μ2^(*),μ2分别是一步和两步的突变率),即更多的“隐性”突变被释放出来。而且父源和母源的三联体和二联体的突变率也有存在差异。随后,将这些突变率数据与已发表的中国其他汉族人口的相关研究进行比较,展现出了STR突变率的时间与区域差异。由于样本量大,本研究中还报告了一些少见的突变事件,例如同卵双胞胎突变和“假四步突变”等。综上所述,本研究通过大量数据获得了接近真实的STR突变率的估计值,不仅可为中国法医DNA数据库和群体遗传学数据库提供重要的基础数据,也对开展法医学个体识别、亲权鉴定和遗传学研究具有重要的意义。
基金supported by the National Key Research and Development Program of China (No. 2017YFC0703200)
文摘Large amounts of cyanide tailings are produced during the cyanidation process in gold extraction,which are hazardous solid wastes due to the toxic cyanide.Pyrite is one of the main minerals in cyanide tailings.The removal of cyanide adsorbed on pyrite by H_2O_2 oxidation under alkaline conditions was investigated in this study.It was found that the removal efficiency was positively correlated with pH from 5 to 12,but remained almost constant when pH was higher than 12.The highest cyanide removal efficiency of 91.10% was achieved by adding no less than 0.6 wt.% of H_2O_2.Cyanide removal was positively correlated with the CN^-adsorption amount between 1.06 and 8.5 mg/g,and temperature between 25 and 85°C.The removal of cyanide adsorbed on pyrite by H_2O_2 oxidation under alkaline conditions was due to the oxidation of pyrite.Hexacyanoferrate,thiocyanate and sulfate were generated with mole ratios of about 2.03:1.12:3.17 during the cyanide removal.?2018 The Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences.
基金Supported by National Science and Technology Support Program of China (No.2006BAI21B11)Scientific Research Innovation Team Project of Beijing University of Chinese Medicine (No.2011-CXTD-14)
文摘OBJECTIVE： To evaluate systematically the clinical efficacy and safety of Qingkailing （QKL） injection in the treatment of acute stroke. METHODS： Searches for randomized controlled trials into acute stroke treated with QKL injection were performed in the China National Knowledge Infrastructure Database, China Science and Technology Journal Database, Wan fang Database, Chinese Biomedical Literature Database, PubMed and Cochrane Library, from January 1979 to March 2013. Two reviewers independently retrieved the RCTs and extracted the information. The Cochrane risk of bias method was used to assess the quality of the included studies, and a Meta-analysis was conducted with Review Manager 5.2 software. RESULTS： A total of 13 studies with 1110 participants were included. The quality of the studies was generally low. The Meta-analysis indicated that the combined use of QKL and Western Medicine was significantly superior to control group therapy in terms of the total effective rate. The relative risk （RR） in the acute cerebral hemorrhage （ACH） sub-group was 1.17 [95% confidence interval （CI） （1.08, 1.26）, P=0.0001]. In the acute cerebral infarction （ACI） sub-group, RR was 1.27 [9.5% CI （1.14, 1.42）, P〈0.0001], and in the ACH and ACI mixed sub-group, RR was 1.34 [95% CI （1.20,1.50）,P〈0.00001]. Additionally, QKL promoted the absorption of hematoma [mean difference （MD）= - 3.73, 95%0 （ - 4.48, - 2.98）, P〈0.000 01], decreased neurological damage in ACI [MD= - 5.60, 95% CI （ - 8.50, - 2.70）, P=0.0002] and ACH [MD= 4.08, 95% CI （ - 8.00, 0.16）, P=0.04], promoted the recovery of awareness [RR=1.56, 95% CI （1.09, 2.21）, P=0.01] and reduced the whole blood viscosity coefficient [MD=- 0.75, 95% CI （ - 1.47, - 0.03）, P=0.04]. There were no adverse drug reactions reported in the included studies. CONCLUSION＂ Based on this systematic review, QKL combined with conventional therapy was effective compared with control treatment. However, because the articles used in the study were not of high quality, further studies should be conducted into the efficacy and safety of QKL in treating acute stroke.
