The Janus kinase and signal transducer and activator of transcription （JAK/STAT） signal transduction pathway is involved in sepsis-induced functional damage to the heart, liver, kidney, and other organs. However, th...The Janus kinase and signal transducer and activator of transcription （JAK/STAT） signal transduction pathway is involved in sepsis-induced functional damage to the heart, liver, kidney, and other organs. However, the cellular and molecular mechanisms underlying sepsis-induced brain damage remain elusive. In the present study, we found severe loss of neurons in the hippocampal CA1 region in rats with sepsis-induced brain damage following intraperitoneal injection of endotoxin, The expression of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 was significantly increased in brain tissues following lipopolysaccharide exposure. AG490 （JAK2 antagonist） and rapamycin （STAT3 antagonist） significantly reduced neuronal loss and suppressed the increased expression of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 in the hippocampal CA1 region in sepsis-induced brain damaged rats. Overall, these data suggest that blockade of the JAK/STAT signal transduction pathway is neuroprotective in sepsis-induced brain damage via the inhibition of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 exoression.展开更多
Contamination is a phenomenon that often occurs in the operation of plant tissue culture,and it is also one of the three major problems in plant tissue culture.Compared with browning and vitrification,contamination is...Contamination is a phenomenon that often occurs in the operation of plant tissue culture,and it is also one of the three major problems in plant tissue culture.Compared with browning and vitrification,contamination is more likely to occur,which brings great harm to scientific research and production practice.Its appearance will greatly affect the normal growth and differentiation of tissue culture materials,and will reduce the yield of cultivated plants to a certain extent.Therefore,we cannot underestimate any step in the tissue culture operation.This study summarized the reasons for its occurrence and how to formulate prevention and control measures based on recent research combined with the actual situation.展开更多
[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle...[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle.展开更多
The reproductive methods of ornamental arrowroot(Maranta arundinacea)mainly include plant division,cutting propagation,truncation and sprout promotion propagation,and tissue culture.Large-scale seedling production mai...The reproductive methods of ornamental arrowroot(Maranta arundinacea)mainly include plant division,cutting propagation,truncation and sprout promotion propagation,and tissue culture.Large-scale seedling production mainly relies on tissue culture.In the process of tissue culture,the culture conditions can be artificially controlled,and the plant materials used for tissue culture grow completely in artificially-provided culture media and microclimate environment,free from the adverse effects of catastrophic climate,seasonal and diurnal changes.Because the conditions are relatively uniform,tissue culture is very beneficial for plant growth and more convenient for stable long-term cultivation and production.In the process of tissue culture,the inoculation process is more important.How can we achieve standardized operation and reduce pollution during the inoculation process is worth exploring.展开更多
Tobacco is a economic pillar in Nanxiong City,and its yield and quality are closely related to climatic conditions.By analyzing the relationship between the growth of tobacco and climatic conditions,and comparing with...Tobacco is a economic pillar in Nanxiong City,and its yield and quality are closely related to climatic conditions.By analyzing the relationship between the growth of tobacco and climatic conditions,and comparing with actual climate in Nanxiong,the best climatic suitability for tobacco planting in Nanxiong was found out.Through comparative analysis between yield,quality of tobacco in recent years and climatic condition in Nanxiong,it was obtained that stable temperature,moderate rainfall and sufficient sunshine were the key meteorological factors to ensure the yield and quality of tobacco,while meteorological disasters such as hail and rainstorm could reduce or even eliminate the yield of tobacco.展开更多
InAs-based interband cascade lasers（ICLs） with InAs plasmon waveguides or InAs/AlSb superlattice（SL） waveguides were demonstrated at emission wavelengths below 4.1 μm. The threshold current densities of the laser...InAs-based interband cascade lasers（ICLs） with InAs plasmon waveguides or InAs/AlSb superlattice（SL） waveguides were demonstrated at emission wavelengths below 4.1 μm. The threshold current densities of the lasers with SL waveguides were 37 A/cm;at 77 K in continuous wave mode. The operation temperature of these lasers reached room temperature in pulsed mode. Compared with the thick InAs n++ plasmon cladding layer, the InAs/AlSb superlattice cladding layers have greater advantages for ICLs with wavelengths less than 4 μm even in InAs based ICLs because in the short-wavelength region they have a higher confinement factor than InAs plasmon waveguides.展开更多
Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium phosphates.In this work,octacalcium phosphate(OCP),a metastable precurs...Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium phosphates.In this work,octacalcium phosphate(OCP),a metastable precursor of biological apatite,was used as a stability model for doping ions(Fe^(3+)and Sr^(2+))with different ionic charges and radii.After treated under hot air at different temperatures(110-200◦C),the phase,morphology,structure,physicochemical properties,protein affinity,ions release,and cytological responses of the ion-doped OCPs were investigated comparatively.The results showed that the collapse of OCP crystals gradually occurred,accompanying with the dehydration of hydrated layers and the disintegration of plate-like crystals as the temperature increased.The collapsed crystals still retained the typical properties of OCP and the potential of conversion into hydroxyapatite.Compared to the undoped OCP,Fe-OCP,and Sr-OCP had lower and higher thermostability respectively,leading to different material surface properties and ions release.The adjusted thermostability of Fe-OCP and Sr-OCP significantly enhanced the adsorption of proteins(BSA and LSZ)and the cytological behavior(adhesion,spreading,proliferation,and osteogenic differentiation)of bone marrow mesenchymal stem cells to a varying extent under the synergistic effects of corresponding surface characteristics and early active ions release.This work paves the way for understanding the modification mechanism of calcium phosphates utilizing ion doping strategy and developing bioactive OCP-based materials for tissue repair.展开更多
文摘The Janus kinase and signal transducer and activator of transcription （JAK/STAT） signal transduction pathway is involved in sepsis-induced functional damage to the heart, liver, kidney, and other organs. However, the cellular and molecular mechanisms underlying sepsis-induced brain damage remain elusive. In the present study, we found severe loss of neurons in the hippocampal CA1 region in rats with sepsis-induced brain damage following intraperitoneal injection of endotoxin, The expression of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 was significantly increased in brain tissues following lipopolysaccharide exposure. AG490 （JAK2 antagonist） and rapamycin （STAT3 antagonist） significantly reduced neuronal loss and suppressed the increased expression of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 in the hippocampal CA1 region in sepsis-induced brain damaged rats. Overall, these data suggest that blockade of the JAK/STAT signal transduction pathway is neuroprotective in sepsis-induced brain damage via the inhibition of toll-like receptor 4, tumor necrosis factor a, and interleukin-6 exoression.
基金Supported by Project of Bureau of Science and Technology of Huizhou Municipality(2021SC040202004,2020SD0409037).
文摘Contamination is a phenomenon that often occurs in the operation of plant tissue culture,and it is also one of the three major problems in plant tissue culture.Compared with browning and vitrification,contamination is more likely to occur,which brings great harm to scientific research and production practice.Its appearance will greatly affect the normal growth and differentiation of tissue culture materials,and will reduce the yield of cultivated plants to a certain extent.Therefore,we cannot underestimate any step in the tissue culture operation.This study summarized the reasons for its occurrence and how to formulate prevention and control measures based on recent research combined with the actual situation.
基金Supported by Project of Bureau of Science and Technology of Huizhou Municipality(2020SD0409037).
文摘[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle.
文摘The reproductive methods of ornamental arrowroot(Maranta arundinacea)mainly include plant division,cutting propagation,truncation and sprout promotion propagation,and tissue culture.Large-scale seedling production mainly relies on tissue culture.In the process of tissue culture,the culture conditions can be artificially controlled,and the plant materials used for tissue culture grow completely in artificially-provided culture media and microclimate environment,free from the adverse effects of catastrophic climate,seasonal and diurnal changes.Because the conditions are relatively uniform,tissue culture is very beneficial for plant growth and more convenient for stable long-term cultivation and production.In the process of tissue culture,the inoculation process is more important.How can we achieve standardized operation and reduce pollution during the inoculation process is worth exploring.
文摘Tobacco is a economic pillar in Nanxiong City,and its yield and quality are closely related to climatic conditions.By analyzing the relationship between the growth of tobacco and climatic conditions,and comparing with actual climate in Nanxiong,the best climatic suitability for tobacco planting in Nanxiong was found out.Through comparative analysis between yield,quality of tobacco in recent years and climatic condition in Nanxiong,it was obtained that stable temperature,moderate rainfall and sufficient sunshine were the key meteorological factors to ensure the yield and quality of tobacco,while meteorological disasters such as hail and rainstorm could reduce or even eliminate the yield of tobacco.
基金Project supported by the National Natural Science Foundation of China(Nos.61790583,61774150,61774151)the National Basic Research Program of China(No.2014CB643903)
文摘InAs-based interband cascade lasers（ICLs） with InAs plasmon waveguides or InAs/AlSb superlattice（SL） waveguides were demonstrated at emission wavelengths below 4.1 μm. The threshold current densities of the lasers with SL waveguides were 37 A/cm;at 77 K in continuous wave mode. The operation temperature of these lasers reached room temperature in pulsed mode. Compared with the thick InAs n++ plasmon cladding layer, the InAs/AlSb superlattice cladding layers have greater advantages for ICLs with wavelengths less than 4 μm even in InAs based ICLs because in the short-wavelength region they have a higher confinement factor than InAs plasmon waveguides.
基金supported by the National Natural Science Foundation of China(Grant 31800791)the Natural Science Foundation of Guangdong Province(Grant 2018030310062)the Fundamental Research Funds for the Central Universities(Grant 21620427).
文摘Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium phosphates.In this work,octacalcium phosphate(OCP),a metastable precursor of biological apatite,was used as a stability model for doping ions(Fe^(3+)and Sr^(2+))with different ionic charges and radii.After treated under hot air at different temperatures(110-200◦C),the phase,morphology,structure,physicochemical properties,protein affinity,ions release,and cytological responses of the ion-doped OCPs were investigated comparatively.The results showed that the collapse of OCP crystals gradually occurred,accompanying with the dehydration of hydrated layers and the disintegration of plate-like crystals as the temperature increased.The collapsed crystals still retained the typical properties of OCP and the potential of conversion into hydroxyapatite.Compared to the undoped OCP,Fe-OCP,and Sr-OCP had lower and higher thermostability respectively,leading to different material surface properties and ions release.The adjusted thermostability of Fe-OCP and Sr-OCP significantly enhanced the adsorption of proteins(BSA and LSZ)and the cytological behavior(adhesion,spreading,proliferation,and osteogenic differentiation)of bone marrow mesenchymal stem cells to a varying extent under the synergistic effects of corresponding surface characteristics and early active ions release.This work paves the way for understanding the modification mechanism of calcium phosphates utilizing ion doping strategy and developing bioactive OCP-based materials for tissue repair.
