This study aimed to investigate the chemical constituents from the flowers of Dendrobium chrysanthum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column c...This study aimed to investigate the chemical constituents from the flowers of Dendrobium chrysanthum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and ElMS）. [Result] Nine compounds were obtained and identified as stigmasterol , β-sitosterol , linoleic acid , stigmast-4-en-3-one , dibutyl phthalate , moscatilin , ergosterol , ergosterol peroxide and daucosterol . [Conclusion] Al compounds were isolated from its flowers for the first time.展开更多
BACKGROUND： Limbic encephalitis is a rare syndrome that specifically affects the limbic system. Magnetic resonance imaging （MRI） has been typically used to detect brain changes in this disease. However, the mechani...BACKGROUND： Limbic encephalitis is a rare syndrome that specifically affects the limbic system. Magnetic resonance imaging （MRI） has been typically used to detect brain changes in this disease. However, the mechanisms of limbic encephalitis-related white matter damage remain poorly understood. OBJECTIVE： To characterize white matter connectivity changes secondary to injuries of the limbic system in limbic encephalitis through combined application of diffusion tensor imaging （DTI） and voxel-based morphometry. DESIGN, TIME AND SETTING： A non-randomized, controlled, clinical, neuroimaging, DTI study was performed at the Department of Radiology, West China Hospital in December 2008. PARTICIPANTS： A male, 46-year-old, limbic encephalitis patient, as well as 11 age- and gender-matched healthy volunteers, were enrolled in the present study. METHODS： MRI was performed on the limbic encephalitis patient using a 3.0T MR scanner. Three-dimensional SPGR Tl-weighted images and DTI were acquired in the patient and controls. Data were analyzed using Matlab 7.0 and SPM2 software. MAIN OUTCOME MEASURES： Results from routine MRI scan with contrast enhancement of patient, as well as fractional anisotropy and mean diffusivity value map differences between patient and controls. RESULTS： Significant symmetric MRI signal intensity abnormalities were observed with routine MRI Affected bilateral hippocampi and amygdala exhibited hypointense signals in TIWI and hyperintense signals in T2 images. The DTI study revealed decreased fractional anisotropy values in the bilateral alveus and fimbria of the hippocampus, bilateral internal and external capsules, white matter of the right prefrontal area, and left corona radiate in the patient compared with normal controls （P 〈 0.001） Significantly increased fractional anisotropy, mean diffusivity, or decreased mean diffusivity were not observed in the patient, compared with controls. CONCLUSION： Secondary white matter damage to the hippocampal afveus and fimbria was apparent in the limbic encephalitis patient. In addition, other white matter fiber injuries surrounded the limbic structures, which were not attributed to secondary limbic system injuries.展开更多
[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chrom...[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis(1H NMR and13 C NMR). [Result] Seven compounds were obtained and identified as 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenan-threne(1), syringaldehyde(2),moscatin(3), gigantol(4), batatasin III(5), tristin(6) and stigmasterol(7). [Conclusion] Compounds 1 and 2 were isolated from this medicinal plant for the first time.展开更多
基金Supported by the National Natural Science Foundation of China(21202066)the Natural Science Foundation of Yunnan Province,China(2012FB156)+1 种基金the Scientific Research Foundation of Department of Education of Yunnan Province,China(2013C247)the Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products(2013G009)
文摘This study aimed to investigate the chemical constituents from the flowers of Dendrobium chrysanthum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and ElMS）. [Result] Nine compounds were obtained and identified as stigmasterol , β-sitosterol , linoleic acid , stigmast-4-en-3-one , dibutyl phthalate , moscatilin , ergosterol , ergosterol peroxide and daucosterol . [Conclusion] Al compounds were isolated from its flowers for the first time.
基金the National Natural Science Foundation of China,No.30800263
文摘BACKGROUND： Limbic encephalitis is a rare syndrome that specifically affects the limbic system. Magnetic resonance imaging （MRI） has been typically used to detect brain changes in this disease. However, the mechanisms of limbic encephalitis-related white matter damage remain poorly understood. OBJECTIVE： To characterize white matter connectivity changes secondary to injuries of the limbic system in limbic encephalitis through combined application of diffusion tensor imaging （DTI） and voxel-based morphometry. DESIGN, TIME AND SETTING： A non-randomized, controlled, clinical, neuroimaging, DTI study was performed at the Department of Radiology, West China Hospital in December 2008. PARTICIPANTS： A male, 46-year-old, limbic encephalitis patient, as well as 11 age- and gender-matched healthy volunteers, were enrolled in the present study. METHODS： MRI was performed on the limbic encephalitis patient using a 3.0T MR scanner. Three-dimensional SPGR Tl-weighted images and DTI were acquired in the patient and controls. Data were analyzed using Matlab 7.0 and SPM2 software. MAIN OUTCOME MEASURES： Results from routine MRI scan with contrast enhancement of patient, as well as fractional anisotropy and mean diffusivity value map differences between patient and controls. RESULTS： Significant symmetric MRI signal intensity abnormalities were observed with routine MRI Affected bilateral hippocampi and amygdala exhibited hypointense signals in TIWI and hyperintense signals in T2 images. The DTI study revealed decreased fractional anisotropy values in the bilateral alveus and fimbria of the hippocampus, bilateral internal and external capsules, white matter of the right prefrontal area, and left corona radiate in the patient compared with normal controls （P 〈 0.001） Significantly increased fractional anisotropy, mean diffusivity, or decreased mean diffusivity were not observed in the patient, compared with controls. CONCLUSION： Secondary white matter damage to the hippocampal afveus and fimbria was apparent in the limbic encephalitis patient. In addition, other white matter fiber injuries surrounded the limbic structures, which were not attributed to secondary limbic system injuries.
基金Supported by Grants from the National Natural Science Foundation of China(No.21202066)the Natural Science Foundation of Yunnan Province,China(No.2012FB156)the Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products(No.2014G011)
文摘[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis(1H NMR and13 C NMR). [Result] Seven compounds were obtained and identified as 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenan-threne(1), syringaldehyde(2),moscatin(3), gigantol(4), batatasin III(5), tristin(6) and stigmasterol(7). [Conclusion] Compounds 1 and 2 were isolated from this medicinal plant for the first time.
