Quantitative investigation on mechanical characteristics of cardiac myocytes has important physiological significance. Based on elastic substrate technique, this paper develops a set of algorithms for high-efficiency ...Quantitative investigation on mechanical characteristics of cardiac myocytes has important physiological significance. Based on elastic substrate technique, this paper develops a set of algorithms for high-efficiency cellular traction recovery. By applying a gradient-based digital image correlation method to track randomly distributed fluorescence microbeads on the deformed substrate induced by single cardiac myocyte, high-resolution substrate displacement field can readily be obtained. By using a numerical algorithm based on the integral Boussinesq solution, cell-substrate tractions are reconstructed in a stable and reliable manner. Finally, spatiotemporal dynamics of a single cardiac myocyte is investigated as it adheres to a polyacrylamide elastic substrate.展开更多
Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whe...Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F cells.Methods:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal cells.The dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA LOC103694972.Si-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F cells.Results:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F cells.These cells exhibited increased cell length and activity compared to the control group.The expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was decreased.However,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell viability.This led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 expression.Additionally,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF pathway.Conclusion:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfibrosis.It negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.展开更多
An acidic gas is an important basic chemical raw material used for synthesizing fertilizers,insecticides,explosives,dyes,and salts.Alternatively,inorganic acidic gases that leak into the air have harmful effects on th...An acidic gas is an important basic chemical raw material used for synthesizing fertilizers,insecticides,explosives,dyes,and salts.Alternatively,inorganic acidic gases that leak into the air have harmful effects on the human health,infrastructure,and cultural relics.Therefore,the demand for inorganic acidic gas sensors for air quality monitoring and management has continuously increased,enabling the development of various sensing technologies.Among them,fiber-optic sensors are promising for acidic gas detection because of their excellent in-situ measurement,resistance to corrosion,anti-electromagnetic interference,long service life,and smart structure.In particular,fiber-optic sensors have proven to be very useful for the in-situ detection and distributed monitoring of multiple gas parameters.However,the sensitivity,selectivity,repeatability,and limits of detection of these sensors can be improved to achieve acceptable performance levels for practical applications.In this review,we introduce fiber-optic sensors based on structured optical fibers and fiber gratings for detecting H_(2)S,SO_(2),NO_(2),CO_(2),and N_(2)O.The structures of the sensing regions,gas-sensitive materials,and measurement principles of these sensors are presented.The sensitivity,selectivity,limit of detection,and response time of the sensors are summarized.Finally,the future of fiber-optic sensors for the detection of inorganic acidic gases is discussed.展开更多
基金supported by the National Basic Research Program (Grant No2007CB935602)the National Natural Science Foundation of China (Grant Nos90607004,10672005 and 10872008)
文摘Quantitative investigation on mechanical characteristics of cardiac myocytes has important physiological significance. Based on elastic substrate technique, this paper develops a set of algorithms for high-efficiency cellular traction recovery. By applying a gradient-based digital image correlation method to track randomly distributed fluorescence microbeads on the deformed substrate induced by single cardiac myocyte, high-resolution substrate displacement field can readily be obtained. By using a numerical algorithm based on the integral Boussinesq solution, cell-substrate tractions are reconstructed in a stable and reliable manner. Finally, spatiotemporal dynamics of a single cardiac myocyte is investigated as it adheres to a polyacrylamide elastic substrate.
基金This work was supported by the Hunan Provincial Education Department General Project Research Fund(No.20C1412)the Hunan Graduate Scientific Research Innovation Project(No.CX2018B474)the National Famous Elderly Chinese Medicine Experts Xinyu Chen Inheritance Workshop Construction Project(No.[2022]75).
文摘Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F cells.Methods:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal cells.The dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA LOC103694972.Si-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F cells.Results:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F cells.These cells exhibited increased cell length and activity compared to the control group.The expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was decreased.However,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell viability.This led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 expression.Additionally,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF pathway.Conclusion:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfibrosis.It negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.
基金the National Natural Science Foundation of China(Grant Nos.52176178 and 52304321)Chongqing Natural Science Foundation Innovation and Development Joint Fund(Municipal Education Commission)Project(Grant No.CSTB2022NSCQLZX0059)+5 种基金Major Scientific and Technological Research Project of Chongqing Municipal Education Commission(Grant No.KJZD-M202201101)Key Research&Development Projects of Sichuan Provincial Science and Technology Plan(Grant No.2023YFS0455)Meishan Science and Technology Plan Project(Grant No.2022ZYZF13)Chongqing Talent Project(Grant No.cstc2022ycjh-bgzxm0241)Innovation Research Group of Universities in Chongqing(Grant No.CXQT21035)Chongqing Postgraduate Innovation Project(Grant No.CYS23661).
文摘An acidic gas is an important basic chemical raw material used for synthesizing fertilizers,insecticides,explosives,dyes,and salts.Alternatively,inorganic acidic gases that leak into the air have harmful effects on the human health,infrastructure,and cultural relics.Therefore,the demand for inorganic acidic gas sensors for air quality monitoring and management has continuously increased,enabling the development of various sensing technologies.Among them,fiber-optic sensors are promising for acidic gas detection because of their excellent in-situ measurement,resistance to corrosion,anti-electromagnetic interference,long service life,and smart structure.In particular,fiber-optic sensors have proven to be very useful for the in-situ detection and distributed monitoring of multiple gas parameters.However,the sensitivity,selectivity,repeatability,and limits of detection of these sensors can be improved to achieve acceptable performance levels for practical applications.In this review,we introduce fiber-optic sensors based on structured optical fibers and fiber gratings for detecting H_(2)S,SO_(2),NO_(2),CO_(2),and N_(2)O.The structures of the sensing regions,gas-sensitive materials,and measurement principles of these sensors are presented.The sensitivity,selectivity,limit of detection,and response time of the sensors are summarized.Finally,the future of fiber-optic sensors for the detection of inorganic acidic gases is discussed.
