[Objectives]This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR(cdPCR)technique.[Methods]Specific primers and probes were designed and synth...[Objectives]This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR(cdPCR)technique.[Methods]Specific primers and probes were designed and synthesized based on the conserved sequence of the heat-resistant nuclease gene nuc of S.aureus.cdPCR was employed to detect S.aureus,and the sensitivity of this technique was systematically assessed in samples exhibiting low levels of contamination.[Results]cdPCR demonstrated precise quantification when the initial concentration of the sample enrichment solution was equal to or greater than 50 CFU/mL.The detection dynamic range extended across at least five orders of magnitude,with a minimum DNA detection limit of 0.2304 pg/μL.In artificially contaminated cheese samples,the method s lower limit of quantification for detecting S.aureus was 8×10^(2) CFU/g.Regression analysis demonstrated that the gene copy number concentration measured by cdPCR exhibited a strong linear correlation with bacterial contamination concentration across a broad range.[Conclusions]The cdPCR method developed in this study demonstrates high sensitivity and robust quantitative capabilities,offering a reliable technical approach for the precise detection of low-level S.aureus contamination in dairy products.展开更多
[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Tota...[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Total flavonoids in milk powder were extracted with ethanol using 45℃ water bath ultrasonication for 60 min.Flavonoids contain a benzopyran ring structure and react with aluminum nitrate in a potassium acetate environment to form a yellow complex.The absorbance of this complex was measured at 420 nm for quantitative determination.[Results]The method exhibited a good linear relationship within the range of 0.2-1.0 mg.The limit of detection(LOD)was 0.05 g/100 g,and the limit of quantification(LOQ)was 0.3 g/100 g.When the spiked amount of total flavonoids was in the range of 0.3% to 1.0%,the spike recovery rates were 99.3% to 103.4%,and the relative standard deviations(RSD s)were less than 3.0%.[Conclusions]This method offers advantages such as rapidness,accuracy,good stability,and high sensitivity,and can be used for the detection of total flavonoid content in milk powder.展开更多
[Objectives]To evaluate the performance of two rapid chromogenic media for the detection of Bacillus cereus in milk powder,and verify the media's inclusivity,exclusivity,and accuracy,and to assess their applicabil...[Objectives]To evaluate the performance of two rapid chromogenic media for the detection of Bacillus cereus in milk powder,and verify the media's inclusivity,exclusivity,and accuracy,and to assess their applicability for the quantitative detection of B.cereus.[Methods]B.cereus in milk powder samples was quantified using two rapid chromogenic media in combination with the national standard method.Agreement between the quantitative results from the three methods was subsequently assessed for agreement via a paired t-test.[Results]No significant differences were observed between the bacterial counts yielded by the two rapid chromogenic media and the national standard method(P>0.05),with excellent agreement between them.[Conclusions]The method of rapid chromogenic culture medium is rapid and simple.展开更多
To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized pur...To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized purge-and-trap(μP&T) device integrating semiconductor refrigeration storage was developed in this work. Water samples were poured into the purge vessels and purged with purified air generated by an air pump. The VOCs in water samples were then separated and preconcentrated with sorbent tubes. After their complete separation and preconcentration, the tubes were subsequently preserved in the semiconductor refrigeration unit of the μP&T device. Notably, the high integration, small size, light weight, and low power consumption of the device makes it easy to be hand-carried to the field and transport by drone from remote locations, significantly enhancing the flexibility of field sampling. The performances of the device were evaluated by comparing analytical figures of merit for the detection of four cyclic volatile methylsiloxanes(cVMSs) in water. Compared to conventional collection and preservation methods, our proposed device preserved the VOCs more consistently in the sorbent tubes, with less than 5% loss of all analytes, and maintained stability for at least 20 days at 4℃. As a proof-of-concept,10 municipal wastewater samples were pretreated using this device with recoveries ranging from 82.5% to 99.9% for the target VOCs.展开更多
This paper reviews the research progress on mold detection technologies in milk and dairy products,including rapid test sheet methods,molecular biological detection techniques,metabolomics detection techniques,enzyme-...This paper reviews the research progress on mold detection technologies in milk and dairy products,including rapid test sheet methods,molecular biological detection techniques,metabolomics detection techniques,enzyme-linked immunosorbent assay(ELISA),and microbial rapid photoelectric detection systems,aiming to provide optimal choices for mold detection.展开更多
Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying ...Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying this manifestation remain poorly understood.In addition to hemostasis,platelets play a crucial role in recognizing pathogens and mediating immune responses.We investigated the mechanisms underlying thrombocytopenia associated with CCHFV infection by analyzing the platelet transcriptome in mice.Interferon-induced transmembrane protein 3(IFITM3),a known antiviral factor,was significantly upregulated.The role of IFITM3 in response to CCHFV infection was characterized using the human megakaryoblast cell line MEG-01,considered a parental cell line of platelets.Although the CCHFV infection rate was limited,MEG-01 cells maintained the infection and replication of CCHFV,leading to increased IFITM3 protein expression.We demonstrated that IFITM3 overexpression efficiently inhibited CCHFV infection,whereas IFITM3 knockout promoted viral infection.An interaction between IFITM3 and the CCHFV glycoprotein Gc was identified,which suppressed CCHFV entry into cells.The IFITM3 CIL-TMD domain is critical for this interaction.These results suggest that IFITM3 is a restriction factor and plays an antiviral role during CCHFV infection.Elevated expression of IFITM3 in platelets indicates that this could be a common mechanism by which platelets protect against viruses,including CCHFV,which may reduce platelet consumption and destruction caused by CCHFV infection.These findings provide valuable insights into the pathogenesis of CCHF-associated thrombocytopenia and offer foundational theoretical support for future therapeutic strategies.展开更多
Rational laboratory layout design and scientific management systems are key to improving overall laboratory efficiency and safety,providing a solid foundation and guarantee for the smooth progress of scientific resear...Rational laboratory layout design and scientific management systems are key to improving overall laboratory efficiency and safety,providing a solid foundation and guarantee for the smooth progress of scientific research.This article addresses a series of issues such as low testing efficiency caused by unreasonable laboratory layouts,incomplete or outdated equipment configurations affecting testing accuracy,and safety hazards arising from the lack of effective laboratory management systems.It conducts an in-depth exploration of the design and planning strategies for physicochemical laboratories.By proposing specific designs and guidelines for the location selection,functional zoning,and layout requirements of physicochemical laboratories,the aim is to optimize laboratory space utilization,enhance testing efficiency,and ensure the advancement of equipment configurations and the accuracy of testing precision.Simultaneously,it emphasizes the establishment of an effective laboratory management system to prevent and control safety hazards,safeguarding the lives of laboratory personnel and ensuring stable laboratory operations.展开更多
Industrial robot application(IRA)provides an opportunity for the low-carbon development of trade.This study focuses on the green revolution of manufacturing export trade,analyzes the mechanism by which IRA affects CO_...Industrial robot application(IRA)provides an opportunity for the low-carbon development of trade.This study focuses on the green revolution of manufacturing export trade,analyzes the mechanism by which IRA affects CO_(2) emissions embodied in manufacturing exports(CIE),and conducts an empirical test based on panel data from 37 countries from 2000 to 2019.This study found that first,IRA can significantly reduce CIE,but there is a U-shaped nexus between the two,which shows a rebound effect.Second,the heterogeneity test demonstrates that in com-parison to both the low-tech and high-tech sectors,IRA in the medium-tech industry can significantly reduce CIE;compared with the low-IRA sectors,the high-IRA sectors exhibit a more obvious reduction.In addition,IRA has a stronger effect on high-carbon-intensity areas.Third,the mechanism test shows that IRA mainly affects CIE through low-carbon technology and productivity effects.Moreover,environmental regulations and the manufacturing in-telligence process positively moderate the nexus between IRA and CIE.Finally,these conclusions provide possible empirical evidence for the smart evolution of the manufacturing industry and the green development of trade.展开更多
According to the announcement of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,the risk of microbial items in frozen drinks is very high,and it is diffic...According to the announcement of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,the risk of microbial items in frozen drinks is very high,and it is difficult to improve.For example,a recent spot check showed that 42 kinds of frozen drinks had microbial indicators exceeding the standard.Part of the reason is that the design of the production workshop is not conducive to the rapid removal of production water,resulting in continuous moisture throughout the workshop,which provides a breeding bed for microorganisms to breed and then contaminates the product.Therefore,research is carried out from the design point of view to fundamentally reduce the moisture in the workshop and build a dry workshop for frozen drinks production,so as to effectively reduce the risk of microbial contamination of frozen drinks.展开更多
This study aimed to determine the effectiveness of comprehensive rehabilitation for patients with aneurysmal subarachnoid hemorrhage (aSAH) and to explore the factors influencing the prognosis of rehabilitation. This ...This study aimed to determine the effectiveness of comprehensive rehabilitation for patients with aneurysmal subarachnoid hemorrhage (aSAH) and to explore the factors influencing the prognosis of rehabilitation. This was a retrospective study. Twenty-five patients with aSAH were treated with physical therapy, occupational therapy, speech therapy, cognitive therapy, music therapy, Chinese acupuncture, hyperbaric oxygen, and transcranial magnetic stimulation. The general data of all patients were collected, and the functional scores at admission were compared with those at discharge. The Mini Mental State Examination, Fugl-Meyer Assessment Scale (FMAS) for motor and balance assessment, Holden Functional Ambulation Classification (FAC), modified Rankin Scale, National Institute of Health Stroke Scale, Modified Barthel Index for activities of daily living (ADL), and Glasgow Outcome Scale were significantly improved among 25 patients with aSAH after 1 month of comprehensive rehabilitation training. Hydrocephalus was an independent factor of the ability to perform ADLs (odds ratio, 0.29;95% confidence interval, 2.03. 3.15;p = 0.000). The improvement of ADLs in aSAH patients was not related to sex, surgical method, aneurysm location, age, or smoking status. Comprehensive and professional rehabilitation is effective for the cognition, movement, walking, ADLs, and functional prognosis of patients with aSAH, while early hydrocephalus may be a risk factor for poor ADLs.展开更多
Introduction: Little is known about the feasibility and effectiveness of rehabilitative treatment for systemic lupus erythematosus (SLE) in individuals with progressive multifocal leukoencephalopathy (PML). We describ...Introduction: Little is known about the feasibility and effectiveness of rehabilitative treatment for systemic lupus erythematosus (SLE) in individuals with progressive multifocal leukoencephalopathy (PML). We describe a patient with SLE complicated by PML and ameliorated by comprehensive rehabilitation. We also review the epidemiology, pathology, imaging characteristics, and treatment of PML. Patient Concerns: We found a patient with SLE with PML improved by multidisciplinary rehabilitation techniques. Diagnoses, Interventions, and Outcomes: We diagnosed a PML with a 13-year history of SLE and lupus nephritis after longtime immunosuppressive therapy. The patient underwent a comprehensive, multifaceted rehabilitation program, including drug therapy, integrated physical therapy, occupational therapy, acupuncture, music therapy, computer-aided cognitive rehabilitation training, and behavioral management training. This rehabilitation program improved her motor function and activities of daily living. Conclusions: Her condition improved in the short term through comprehensive rehabilitation, including physical, speech, and cognitive therapy. Therefore, we recommend comprehensive rehabilitation to improve the function and activities of daily living in patients with PML.展开更多
Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Provinc...Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Province is one of the major SFTS epidemic areas in the central part of China.This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017.Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains.It was suggested that five different SFTSV genotypes were circulating in Hubei,and 15 reassortment patterns and migration pathways correlated with each genotype were identified,which was more than previously recognized.Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions.Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1.Subsequently,amino acid variations specific for genotype(s),strain(s),or cluster(s)were inspected,which may be related to differential biological activity of SFTSV strains/genotypes.In conclusion,we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution.It provided an in-depth insight into SFTSV evolution,raising concerns for the use of proper SFTSV strains in future studies.展开更多
Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been...Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord.展开更多
In the south of the Ordos Basin, the oil source of the Upper Triassic Yanchang Formation is confused all the time, which affects further exploration. In this study, oil sources from the oil layers of Ch6, Ch8 and Ch9 ...In the south of the Ordos Basin, the oil source of the Upper Triassic Yanchang Formation is confused all the time, which affects further exploration. In this study, oil sources from the oil layers of Ch6, Ch8 and Ch9 are all analyzed and confirmed. Through their carbon isotope value and biomarkers, characteristics of crude oils from the Yanchang Formation are analyzed. Then, the oil–source relation is discussed, with the source rocks' features.Finally, the oil–source relation is calculated through cluster analysis. It is believed that the oils from the Yanchang Formation deposit in a similar redox environment, with weak oxidation–weak reduction, and have all entered maturity stage. Ch9 crude oil is more mature than crude oils from Ch6 and Ch8, and has more advanced plants and fewer algae. Gas chromatography(GC) and gas chromatography–mass spectrometry(GC–MS) analysis show that crude oils from Ch6 and Ch8 may come from Ch7, and Ch9 crude oil may not. Cluster analysis displays that crude oils from Ch6 and Ch8 have closer squared Euclidean distance with Ch7 source rocks than Ch9 crude oil does,indicating crude oils from Ch6 and Ch8 stem from Ch7 source rocks. And Ch9 crude oil has rather close squared Euclidean distance with Ch9 source rocks, illustrating Ch9 crude oil may be from Ch9 source rocks. This research may provide the theoretical basis for the next exploration deploy in the south of Ordos Basin.展开更多
Using trace elements to reconstruct paleoenvironment is a current hot topic in geochemistry. Through analytical tests of oil yield, ash yield, calorific value, total sulfur, major elements, trace elements, and X-ray d...Using trace elements to reconstruct paleoenvironment is a current hot topic in geochemistry. Through analytical tests of oil yield, ash yield, calorific value, total sulfur, major elements, trace elements, and X-ray diffraction, the quality, mineral content, occurrence mode of elements, and paleoenvironment of the Zhangjiatan oil shale of the Triassic Yanchang Formation in the southern Ordos Basin were studied. The analyses revealed relatively high oil yield(average 6.63%) and medium quality. The mineral content in the oil shale was mainly clay minerals,quartz, feldspar, and pyrite; an illite–smectite mixed layer comprised the major proportion of clay minerals. Compared with marine oil shale in China, the Zhangjiatan oil shale had higher contents of quartz, feldspar, and clay minerals, and lower calcite content. Silica was mainly in quartz and Fe was associated with organic matter, which is different from marine oil shale. The form of calcium varied. Cluster analyses indicated that Fe, Cu, U, V, Zn, As,Cs, Cd, Mo, Ga, Pb, Co, Ni, Cr, Sc, P, and Mn are associated with organic matter while Ca, Na, Sr, Ba, Si, Zr, K,Al, B, Mg, and Ti are mostly terrigenous. Sr/Cu, Ba/Al, V/(V+ Ni), U/Th, AU, and δU of oil shale samples suggest the paleoclimate was warm and humid, paleoproductivity of the lake was relatively high during deposition of the shale—which mainly occurred in fresh water—and the paleo-redox condition was dominated by reducing conditions. Fe/Ti ratios of the oil shale samples suggest clear hydrothermal influence in the eastern portion of the study area and less conspicuous hydrothermal influence in the western portion.展开更多
Severe fever with thrombocytopenia syndrome(SFTS),caused by SFTS virus(SFTSV)infection,was first reported in 2010 in China with an initial fatality of up to 30%.The laboratory confirmation of SFTSV infection in terms ...Severe fever with thrombocytopenia syndrome(SFTS),caused by SFTS virus(SFTSV)infection,was first reported in 2010 in China with an initial fatality of up to 30%.The laboratory confirmation of SFTSV infection in terms of detection of viral RNA or antibody levels is critical for SFTS diagnosis and therapy.In this study,a new luciferase immunoprecipitation system(LIPS)assay based on p REN2 plasmid expressing SFTSV NP gene and tagged with Renilla luciferase(Rluc),was established and used to investigate the levels of antibody responses to SFTSV.Totally 464 serum samples from febrile patients were collected in the hospital of Shaoxing City in Zhejiang Province in 2019.The results showed that 82 of the 464 patients(17.7%)had antibody response to SFTSV,which were further supported by immunofluorescence assays(IFAs).Further,q RT-PCR and microneutralization tests showed that among the 82 positive cases,15 patients had viremia,10 patients had neutralizing antibody,and one had both(totally 26 patient).However,none of these patients were diagnosed as SFTS in the hospital probably because of their mild symptoms or subclinical manifestations.All the results indicated that at least the 26 patients having viremia or neutralizing antibody were the missed diagnosis of SFTS cases.The findings suggested the occurrence of SFTS and the SFTS incidence were higher than the reported level in Shaoxing in 2019,and that LIPS may provide an alternative strategy to confirm SFTSV infection in the laboratory.展开更多
A total of 66 samples （from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, aa 13 cases with optic neuritis） were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an...A total of 66 samples （from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, aa 13 cases with optic neuritis） were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay. The sensitivities and specificities of the two assays were similar. We further analyzed an additional 68 patients and 93 healthy controls using the enzyme-linked immunosorbent assay. A Kappa test showed good consistency between the two methods in terms of detection of anti-aquaporin-4 antibody in the se of neuromyelitis optica patients. No significant correlations were identified with onset age or disea duration, suggesting that aquaporin-4 antibody is a good marker for neuromyelitis optica. The enzyme-linked immunosorbent assay can be used for quantifying aquaporin-4 antibody concentrations and may be useful to dynamically monitor changes in the levels of aquaporin-4 antibody during disease duration.展开更多
The rational construction of electrocatalysts with desired features is significant but challenging for superior water splitting at high current density. Herein, amorphous Co Ni S nanosheets are synthesized on nickel f...The rational construction of electrocatalysts with desired features is significant but challenging for superior water splitting at high current density. Herein, amorphous Co Ni S nanosheets are synthesized on nickel foam(NF) through a facile structure evolution strategy and present advanced performance at high current densities in water splitting. The high catalytic activity can be attributed to the sufficient active sites exposed by the flexible amorphous configuration. Moreover, the hydrophilicity and aerophobicity of a-CoNiS/NF promote surface wettability of the self-supporting electrode and avoid the aggregation of bubbles, which expedites the diffusion of electrolyte and facilitates the mass transfer. As a result, the optimized electrode demonstrates low overpotentials of 289 and 434 m V at 500 m A/cm^(2) under alkaline conditions for hydrogen evolution reaction(HER) and oxygen evolution reaction(OER), respectively. Impressively, an electrolytic water splitting cell assembled by bifunctional a-Co Ni S/NF operates with a low cell voltage of 1.46 V@10 mA/cm^(2) and reaches 1.79 V at 500 mA/cm^(2). The strategy sheds light on a competitive platform for the reasonable design of non-precious-metal electrocatalysts under high current density.展开更多
Hydrothermal fluid activity during sedimenta- tion of the Triassic Yanchang Formation in the Ordos Basin and the impact of said activity on formation and preservation conditions of source rocks have received little at...Hydrothermal fluid activity during sedimenta- tion of the Triassic Yanchang Formation in the Ordos Basin and the impact of said activity on formation and preservation conditions of source rocks have received little attention. Oil yield, major element, trace element, rare earth element, and total sulfur （TS） data from the oil shale within the Yanchang are here presented and discussed in the context of hydrothermal influence. Oil shale samples returned relatively high total organic carbon （TOC）, in the range of 4.69%-25.48%. A high correlation between TS and TOC suggests TS in the oil shale is dominated by organic sulfur and affected by organic matter. The low Al/ Si ratio of oil shale samples implies quartz is a major mineralogical component. Si/（Si ＋ Al ＋ Fe） values suggest close proximity of the oil shale to a terrigenous source. δEu; Fe versus Mn versus （Cu ＋ Co ＋ Ni）× 10; and SiO2/（K2O ＋ Na2O） versus MnO/TiO2, Fe/Ti, and （Fe ＋ Mn）/Ti are evidence of hydrothermal fluid activity during oil shale sedimentation, and δU and U/Th of the oil shale indicate reducing conditions. The Sr/Ba of oil shale samples suggests fresh-water deposition. The high correlations of Fe/Ti and （Fe ＋ Mn）/Ti with δU, U/Th, and TS demonstrate that hydrothermal fluid activity promotes reducing conditions. Sr/Ba ratios had low correlation with Fe/Ti and （Fe ＋ Mn）/Ti, implying that hydrothermal fluid activity had little impact （Fe ＋ Mn）/Ti, δU, U/Th, and on paleosalinity. Fe/Ti, Cu ＋ Pb ＋ Zn all exhibited high positive correlation coefficients with TOC in oil shale samples, suggesting that more intense hydrothermal fluid activity improves conditions in favor of formation and preservation of organic matter.展开更多
At present,many researchers focused on the point-of-care testing（POCT）,a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered...At present,many researchers focused on the point-of-care testing（POCT）,a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection.Herein,we developed a newly QD-immune fluorescence test strips（QD-IFTS） based on quantum dots（QDs） as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen（CEA）,a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis.And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS.Under the optimized reaction conditions,chromatographic time of the newly QD-IFTS was only25 min,sample volume of the newly QD-IFTS was only 40 m L and the LOD of the newly QD-IFTS was 0.72 ng/m L.In addition,the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples,and the results revealed great potential in clinical POCT of other biomarkers.展开更多
基金Supported by Science and Technology Program of Inner Mongolia Autonomous Region"Research and Demonstration of Novel Molecular Biological Identification Technology for Multiple Source Components in Milk and Dairy Products"(2025YFSH0029).
文摘[Objectives]This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR(cdPCR)technique.[Methods]Specific primers and probes were designed and synthesized based on the conserved sequence of the heat-resistant nuclease gene nuc of S.aureus.cdPCR was employed to detect S.aureus,and the sensitivity of this technique was systematically assessed in samples exhibiting low levels of contamination.[Results]cdPCR demonstrated precise quantification when the initial concentration of the sample enrichment solution was equal to or greater than 50 CFU/mL.The detection dynamic range extended across at least five orders of magnitude,with a minimum DNA detection limit of 0.2304 pg/μL.In artificially contaminated cheese samples,the method s lower limit of quantification for detecting S.aureus was 8×10^(2) CFU/g.Regression analysis demonstrated that the gene copy number concentration measured by cdPCR exhibited a strong linear correlation with bacterial contamination concentration across a broad range.[Conclusions]The cdPCR method developed in this study demonstrates high sensitivity and robust quantitative capabilities,offering a reliable technical approach for the precise detection of low-level S.aureus contamination in dairy products.
基金Supported by Key Research and Development and Achievement Transformation Plan Project of Inner Mongolia Autonomous Region(2023YFHH0093).
文摘[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Total flavonoids in milk powder were extracted with ethanol using 45℃ water bath ultrasonication for 60 min.Flavonoids contain a benzopyran ring structure and react with aluminum nitrate in a potassium acetate environment to form a yellow complex.The absorbance of this complex was measured at 420 nm for quantitative determination.[Results]The method exhibited a good linear relationship within the range of 0.2-1.0 mg.The limit of detection(LOD)was 0.05 g/100 g,and the limit of quantification(LOQ)was 0.3 g/100 g.When the spiked amount of total flavonoids was in the range of 0.3% to 1.0%,the spike recovery rates were 99.3% to 103.4%,and the relative standard deviations(RSD s)were less than 3.0%.[Conclusions]This method offers advantages such as rapidness,accuracy,good stability,and high sensitivity,and can be used for the detection of total flavonoid content in milk powder.
基金Supported by the Inner Mongolia Autonomous Region's Key Research and Achievement Transformation Plan(2025YFSH0029).
文摘[Objectives]To evaluate the performance of two rapid chromogenic media for the detection of Bacillus cereus in milk powder,and verify the media's inclusivity,exclusivity,and accuracy,and to assess their applicability for the quantitative detection of B.cereus.[Methods]B.cereus in milk powder samples was quantified using two rapid chromogenic media in combination with the national standard method.Agreement between the quantitative results from the three methods was subsequently assessed for agreement via a paired t-test.[Results]No significant differences were observed between the bacterial counts yielded by the two rapid chromogenic media and the national standard method(P>0.05),with excellent agreement between them.[Conclusions]The method of rapid chromogenic culture medium is rapid and simple.
基金the National Natural Science Foundation of China (No. 22306146)the PhD Scientific Research Startup Foundation of Xihua University (No. RX2200002003) for their financial support。
文摘To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized purge-and-trap(μP&T) device integrating semiconductor refrigeration storage was developed in this work. Water samples were poured into the purge vessels and purged with purified air generated by an air pump. The VOCs in water samples were then separated and preconcentrated with sorbent tubes. After their complete separation and preconcentration, the tubes were subsequently preserved in the semiconductor refrigeration unit of the μP&T device. Notably, the high integration, small size, light weight, and low power consumption of the device makes it easy to be hand-carried to the field and transport by drone from remote locations, significantly enhancing the flexibility of field sampling. The performances of the device were evaluated by comparing analytical figures of merit for the detection of four cyclic volatile methylsiloxanes(cVMSs) in water. Compared to conventional collection and preservation methods, our proposed device preserved the VOCs more consistently in the sorbent tubes, with less than 5% loss of all analytes, and maintained stability for at least 20 days at 4℃. As a proof-of-concept,10 municipal wastewater samples were pretreated using this device with recoveries ranging from 82.5% to 99.9% for the target VOCs.
基金Supported by Research Project on Food Detection Technology Innovation and Standard Integration 2024(YNXM-2024-FW-019).
文摘This paper reviews the research progress on mold detection technologies in milk and dairy products,including rapid test sheet methods,molecular biological detection techniques,metabolomics detection techniques,enzyme-linked immunosorbent assay(ELISA),and microbial rapid photoelectric detection systems,aiming to provide optimal choices for mold detection.
基金supported by the the National Key R&D Program of China(2024YFC2310000)the Key Project of Key Laboratory of VirologyBiosafety in the Wuhan Institute of Virology,Chinese Academy of Sciences(2024JZZD-02),the Youth Project of the Wuhan Institute of Virology,Chinese Academy of Sciences(2023QNTJ-03)+2 种基金the"Open Competition for Selecting the Best Candidates"Project of the Wuhan East Lake New Technology Development Zone(2022KJB117)the Natural Science Foundation of Hubei Province(2024AFB986)the Medical Science Research Project of Wuhan Health Commission(WX23B09).
文摘Crimean-Congo hemorrhagic fever(CCHF)is a hemorrhagic fever caused by infection with the CCHF virus(CCHFV)and has a mortality rate of up to 30%.Thrombocytopenia is a hallmark of CCHF;however,the mechanisms underlying this manifestation remain poorly understood.In addition to hemostasis,platelets play a crucial role in recognizing pathogens and mediating immune responses.We investigated the mechanisms underlying thrombocytopenia associated with CCHFV infection by analyzing the platelet transcriptome in mice.Interferon-induced transmembrane protein 3(IFITM3),a known antiviral factor,was significantly upregulated.The role of IFITM3 in response to CCHFV infection was characterized using the human megakaryoblast cell line MEG-01,considered a parental cell line of platelets.Although the CCHFV infection rate was limited,MEG-01 cells maintained the infection and replication of CCHFV,leading to increased IFITM3 protein expression.We demonstrated that IFITM3 overexpression efficiently inhibited CCHFV infection,whereas IFITM3 knockout promoted viral infection.An interaction between IFITM3 and the CCHFV glycoprotein Gc was identified,which suppressed CCHFV entry into cells.The IFITM3 CIL-TMD domain is critical for this interaction.These results suggest that IFITM3 is a restriction factor and plays an antiviral role during CCHFV infection.Elevated expression of IFITM3 in platelets indicates that this could be a common mechanism by which platelets protect against viruses,including CCHFV,which may reduce platelet consumption and destruction caused by CCHFV infection.These findings provide valuable insights into the pathogenesis of CCHF-associated thrombocytopenia and offer foundational theoretical support for future therapeutic strategies.
文摘Rational laboratory layout design and scientific management systems are key to improving overall laboratory efficiency and safety,providing a solid foundation and guarantee for the smooth progress of scientific research.This article addresses a series of issues such as low testing efficiency caused by unreasonable laboratory layouts,incomplete or outdated equipment configurations affecting testing accuracy,and safety hazards arising from the lack of effective laboratory management systems.It conducts an in-depth exploration of the design and planning strategies for physicochemical laboratories.By proposing specific designs and guidelines for the location selection,functional zoning,and layout requirements of physicochemical laboratories,the aim is to optimize laboratory space utilization,enhance testing efficiency,and ensure the advancement of equipment configurations and the accuracy of testing precision.Simultaneously,it emphasizes the establishment of an effective laboratory management system to prevent and control safety hazards,safeguarding the lives of laboratory personnel and ensuring stable laboratory operations.
基金the National Social Science Foundation of China(Grant No.23FGLB024)Special Project on“Promoting High-Quality Development through the Integration of the Yangtze River Delta”of Shaoxing University(Grant No.2024CSJYB01)to provide fund for the study。
文摘Industrial robot application(IRA)provides an opportunity for the low-carbon development of trade.This study focuses on the green revolution of manufacturing export trade,analyzes the mechanism by which IRA affects CO_(2) emissions embodied in manufacturing exports(CIE),and conducts an empirical test based on panel data from 37 countries from 2000 to 2019.This study found that first,IRA can significantly reduce CIE,but there is a U-shaped nexus between the two,which shows a rebound effect.Second,the heterogeneity test demonstrates that in com-parison to both the low-tech and high-tech sectors,IRA in the medium-tech industry can significantly reduce CIE;compared with the low-IRA sectors,the high-IRA sectors exhibit a more obvious reduction.In addition,IRA has a stronger effect on high-carbon-intensity areas.Third,the mechanism test shows that IRA mainly affects CIE through low-carbon technology and productivity effects.Moreover,environmental regulations and the manufacturing in-telligence process positively moderate the nexus between IRA and CIE.Finally,these conclusions provide possible empirical evidence for the smart evolution of the manufacturing industry and the green development of trade.
文摘According to the announcement of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,the risk of microbial items in frozen drinks is very high,and it is difficult to improve.For example,a recent spot check showed that 42 kinds of frozen drinks had microbial indicators exceeding the standard.Part of the reason is that the design of the production workshop is not conducive to the rapid removal of production water,resulting in continuous moisture throughout the workshop,which provides a breeding bed for microorganisms to breed and then contaminates the product.Therefore,research is carried out from the design point of view to fundamentally reduce the moisture in the workshop and build a dry workshop for frozen drinks production,so as to effectively reduce the risk of microbial contamination of frozen drinks.
文摘This study aimed to determine the effectiveness of comprehensive rehabilitation for patients with aneurysmal subarachnoid hemorrhage (aSAH) and to explore the factors influencing the prognosis of rehabilitation. This was a retrospective study. Twenty-five patients with aSAH were treated with physical therapy, occupational therapy, speech therapy, cognitive therapy, music therapy, Chinese acupuncture, hyperbaric oxygen, and transcranial magnetic stimulation. The general data of all patients were collected, and the functional scores at admission were compared with those at discharge. The Mini Mental State Examination, Fugl-Meyer Assessment Scale (FMAS) for motor and balance assessment, Holden Functional Ambulation Classification (FAC), modified Rankin Scale, National Institute of Health Stroke Scale, Modified Barthel Index for activities of daily living (ADL), and Glasgow Outcome Scale were significantly improved among 25 patients with aSAH after 1 month of comprehensive rehabilitation training. Hydrocephalus was an independent factor of the ability to perform ADLs (odds ratio, 0.29;95% confidence interval, 2.03. 3.15;p = 0.000). The improvement of ADLs in aSAH patients was not related to sex, surgical method, aneurysm location, age, or smoking status. Comprehensive and professional rehabilitation is effective for the cognition, movement, walking, ADLs, and functional prognosis of patients with aSAH, while early hydrocephalus may be a risk factor for poor ADLs.
文摘Introduction: Little is known about the feasibility and effectiveness of rehabilitative treatment for systemic lupus erythematosus (SLE) in individuals with progressive multifocal leukoencephalopathy (PML). We describe a patient with SLE complicated by PML and ameliorated by comprehensive rehabilitation. We also review the epidemiology, pathology, imaging characteristics, and treatment of PML. Patient Concerns: We found a patient with SLE with PML improved by multidisciplinary rehabilitation techniques. Diagnoses, Interventions, and Outcomes: We diagnosed a PML with a 13-year history of SLE and lupus nephritis after longtime immunosuppressive therapy. The patient underwent a comprehensive, multifaceted rehabilitation program, including drug therapy, integrated physical therapy, occupational therapy, acupuncture, music therapy, computer-aided cognitive rehabilitation training, and behavioral management training. This rehabilitation program improved her motor function and activities of daily living. Conclusions: Her condition improved in the short term through comprehensive rehabilitation, including physical, speech, and cognitive therapy. Therefore, we recommend comprehensive rehabilitation to improve the function and activities of daily living in patients with PML.
基金supported by the National Key R&D Programme of China(2018ZX10734404-010)the Nature Science Foundation of Hubei Province(2019CFB790 and 2018CFB471)+2 种基金the Innovation Team Project of Hubei Provincial Health Commission(WJ2019C003)the Open Research Fund of State Key Laboratory of Virology(2018IOV004)the Special Project of Technical Conditions(2060503)。
文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Province is one of the major SFTS epidemic areas in the central part of China.This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017.Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains.It was suggested that five different SFTSV genotypes were circulating in Hubei,and 15 reassortment patterns and migration pathways correlated with each genotype were identified,which was more than previously recognized.Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions.Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1.Subsequently,amino acid variations specific for genotype(s),strain(s),or cluster(s)were inspected,which may be related to differential biological activity of SFTSV strains/genotypes.In conclusion,we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution.It provided an in-depth insight into SFTSV evolution,raising concerns for the use of proper SFTSV strains in future studies.
基金supported by the Foundation for Distinguished Young Talents in Higher Education of Guangdong Province, China (2016KQNCX019 and 2016KQNCX027)the National Natural Science Foundation of China (31571041)+1 种基金the Guangdong Provincial Department of Education Innovating Strong National Engineering Major Project (2014GKXM031)Guangdong Provincial Universities and Colleges Pearl River Scholar Funded Scheme (2016)
文摘Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord.
基金supported with funding from the National Natural Science Foundation of China(No.41173055)
文摘In the south of the Ordos Basin, the oil source of the Upper Triassic Yanchang Formation is confused all the time, which affects further exploration. In this study, oil sources from the oil layers of Ch6, Ch8 and Ch9 are all analyzed and confirmed. Through their carbon isotope value and biomarkers, characteristics of crude oils from the Yanchang Formation are analyzed. Then, the oil–source relation is discussed, with the source rocks' features.Finally, the oil–source relation is calculated through cluster analysis. It is believed that the oils from the Yanchang Formation deposit in a similar redox environment, with weak oxidation–weak reduction, and have all entered maturity stage. Ch9 crude oil is more mature than crude oils from Ch6 and Ch8, and has more advanced plants and fewer algae. Gas chromatography(GC) and gas chromatography–mass spectrometry(GC–MS) analysis show that crude oils from Ch6 and Ch8 may come from Ch7, and Ch9 crude oil may not. Cluster analysis displays that crude oils from Ch6 and Ch8 have closer squared Euclidean distance with Ch7 source rocks than Ch9 crude oil does,indicating crude oils from Ch6 and Ch8 stem from Ch7 source rocks. And Ch9 crude oil has rather close squared Euclidean distance with Ch9 source rocks, illustrating Ch9 crude oil may be from Ch9 source rocks. This research may provide the theoretical basis for the next exploration deploy in the south of Ordos Basin.
基金supported by funding from the National Natural Science Foundation of China (No.41173055)the Fundamental Research Funds for the Central Universities (No.310827172101)
文摘Using trace elements to reconstruct paleoenvironment is a current hot topic in geochemistry. Through analytical tests of oil yield, ash yield, calorific value, total sulfur, major elements, trace elements, and X-ray diffraction, the quality, mineral content, occurrence mode of elements, and paleoenvironment of the Zhangjiatan oil shale of the Triassic Yanchang Formation in the southern Ordos Basin were studied. The analyses revealed relatively high oil yield(average 6.63%) and medium quality. The mineral content in the oil shale was mainly clay minerals,quartz, feldspar, and pyrite; an illite–smectite mixed layer comprised the major proportion of clay minerals. Compared with marine oil shale in China, the Zhangjiatan oil shale had higher contents of quartz, feldspar, and clay minerals, and lower calcite content. Silica was mainly in quartz and Fe was associated with organic matter, which is different from marine oil shale. The form of calcium varied. Cluster analyses indicated that Fe, Cu, U, V, Zn, As,Cs, Cd, Mo, Ga, Pb, Co, Ni, Cr, Sc, P, and Mn are associated with organic matter while Ca, Na, Sr, Ba, Si, Zr, K,Al, B, Mg, and Ti are mostly terrigenous. Sr/Cu, Ba/Al, V/(V+ Ni), U/Th, AU, and δU of oil shale samples suggest the paleoclimate was warm and humid, paleoproductivity of the lake was relatively high during deposition of the shale—which mainly occurred in fresh water—and the paleo-redox condition was dominated by reducing conditions. Fe/Ti ratios of the oil shale samples suggest clear hydrothermal influence in the eastern portion of the study area and less conspicuous hydrothermal influence in the western portion.
基金supported by the National Program on Key Research Project of China(2018YFE0200400,2019YFC1200700)the National Natural Science Foundation of China(U20A20135)+1 种基金the Strategic Biological Resources Capacity Building Project of Chinese Academy of Sciences(KFJ-BRP-017-06)the Key deployment projects of Chinese Academy of Sciences(KJZD-SW-L11)
文摘Severe fever with thrombocytopenia syndrome(SFTS),caused by SFTS virus(SFTSV)infection,was first reported in 2010 in China with an initial fatality of up to 30%.The laboratory confirmation of SFTSV infection in terms of detection of viral RNA or antibody levels is critical for SFTS diagnosis and therapy.In this study,a new luciferase immunoprecipitation system(LIPS)assay based on p REN2 plasmid expressing SFTSV NP gene and tagged with Renilla luciferase(Rluc),was established and used to investigate the levels of antibody responses to SFTSV.Totally 464 serum samples from febrile patients were collected in the hospital of Shaoxing City in Zhejiang Province in 2019.The results showed that 82 of the 464 patients(17.7%)had antibody response to SFTSV,which were further supported by immunofluorescence assays(IFAs).Further,q RT-PCR and microneutralization tests showed that among the 82 positive cases,15 patients had viremia,10 patients had neutralizing antibody,and one had both(totally 26 patient).However,none of these patients were diagnosed as SFTS in the hospital probably because of their mild symptoms or subclinical manifestations.All the results indicated that at least the 26 patients having viremia or neutralizing antibody were the missed diagnosis of SFTS cases.The findings suggested the occurrence of SFTS and the SFTS incidence were higher than the reported level in Shaoxing in 2019,and that LIPS may provide an alternative strategy to confirm SFTSV infection in the laboratory.
基金the grants from the Ministry of Sciences and Technology of China, No. 2006AA02A408, 2008ZX09312-014
文摘A total of 66 samples （from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, aa 13 cases with optic neuritis） were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay. The sensitivities and specificities of the two assays were similar. We further analyzed an additional 68 patients and 93 healthy controls using the enzyme-linked immunosorbent assay. A Kappa test showed good consistency between the two methods in terms of detection of anti-aquaporin-4 antibody in the se of neuromyelitis optica patients. No significant correlations were identified with onset age or disea duration, suggesting that aquaporin-4 antibody is a good marker for neuromyelitis optica. The enzyme-linked immunosorbent assay can be used for quantifying aquaporin-4 antibody concentrations and may be useful to dynamically monitor changes in the levels of aquaporin-4 antibody during disease duration.
基金supported by the National Natural Science Foundation of China (Nos. 51871119, 22101132, and 22075141)Scientific and Technological Innovation Special Fund for Carbon Peak and Carbon Neutrality of Jiangsu Province (No. BK20220039)+4 种基金Jiangsu Provincial Founds for Natural Science Foundation (No. BK20210311)China Postdoctoral Science Foundation (Nos. 2018M640481 and 2019T120426)the Natural Science Foundation of Jiangsu Province (No. BK20210311)Jiangsu Postdoctoral Research Fund (No. 2019K003)the Postgraduate Research & Practice Innovation Program of NUAA (No. xcxjh20210607)。
文摘The rational construction of electrocatalysts with desired features is significant but challenging for superior water splitting at high current density. Herein, amorphous Co Ni S nanosheets are synthesized on nickel foam(NF) through a facile structure evolution strategy and present advanced performance at high current densities in water splitting. The high catalytic activity can be attributed to the sufficient active sites exposed by the flexible amorphous configuration. Moreover, the hydrophilicity and aerophobicity of a-CoNiS/NF promote surface wettability of the self-supporting electrode and avoid the aggregation of bubbles, which expedites the diffusion of electrolyte and facilitates the mass transfer. As a result, the optimized electrode demonstrates low overpotentials of 289 and 434 m V at 500 m A/cm^(2) under alkaline conditions for hydrogen evolution reaction(HER) and oxygen evolution reaction(OER), respectively. Impressively, an electrolytic water splitting cell assembled by bifunctional a-Co Ni S/NF operates with a low cell voltage of 1.46 V@10 mA/cm^(2) and reaches 1.79 V at 500 mA/cm^(2). The strategy sheds light on a competitive platform for the reasonable design of non-precious-metal electrocatalysts under high current density.
基金supported with funding from the National Natural Science Foundation of China (No. 41173055)the Fundamental Research Funds for the Central Universities (No. 310827172101)
文摘Hydrothermal fluid activity during sedimenta- tion of the Triassic Yanchang Formation in the Ordos Basin and the impact of said activity on formation and preservation conditions of source rocks have received little attention. Oil yield, major element, trace element, rare earth element, and total sulfur （TS） data from the oil shale within the Yanchang are here presented and discussed in the context of hydrothermal influence. Oil shale samples returned relatively high total organic carbon （TOC）, in the range of 4.69%-25.48%. A high correlation between TS and TOC suggests TS in the oil shale is dominated by organic sulfur and affected by organic matter. The low Al/ Si ratio of oil shale samples implies quartz is a major mineralogical component. Si/（Si ＋ Al ＋ Fe） values suggest close proximity of the oil shale to a terrigenous source. δEu; Fe versus Mn versus （Cu ＋ Co ＋ Ni）× 10; and SiO2/（K2O ＋ Na2O） versus MnO/TiO2, Fe/Ti, and （Fe ＋ Mn）/Ti are evidence of hydrothermal fluid activity during oil shale sedimentation, and δU and U/Th of the oil shale indicate reducing conditions. The Sr/Ba of oil shale samples suggests fresh-water deposition. The high correlations of Fe/Ti and （Fe ＋ Mn）/Ti with δU, U/Th, and TS demonstrate that hydrothermal fluid activity promotes reducing conditions. Sr/Ba ratios had low correlation with Fe/Ti and （Fe ＋ Mn）/Ti, implying that hydrothermal fluid activity had little impact （Fe ＋ Mn）/Ti, δU, U/Th, and on paleosalinity. Fe/Ti, Cu ＋ Pb ＋ Zn all exhibited high positive correlation coefficients with TOC in oil shale samples, suggesting that more intense hydrothermal fluid activity improves conditions in favor of formation and preservation of organic matter.
基金financially supported by the National Natural Science Foundation of China(Nos.51373117,51303126 and 31600800)Tianjin Natural Science and Technology Foundation(No.16ZXMJSY00010)
文摘At present,many researchers focused on the point-of-care testing（POCT）,a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection.Herein,we developed a newly QD-immune fluorescence test strips（QD-IFTS） based on quantum dots（QDs） as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen（CEA）,a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis.And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS.Under the optimized reaction conditions,chromatographic time of the newly QD-IFTS was only25 min,sample volume of the newly QD-IFTS was only 40 m L and the LOD of the newly QD-IFTS was 0.72 ng/m L.In addition,the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples,and the results revealed great potential in clinical POCT of other biomarkers.
