Aim: The clinical value of FibroScan in evaluation of hepatic fibrosis was assessed in Chinese patients with nonalcoholic fatty liver disease (NAFLD). Methods: Liver stiffness and other NAFLD related variables were me...Aim: The clinical value of FibroScan in evaluation of hepatic fibrosis was assessed in Chinese patients with nonalcoholic fatty liver disease (NAFLD). Methods: Liver stiffness and other NAFLD related variables were measured in NAFLD patients with either elevated or normal hyaluronic acid (HA) levels, and in healthy volunteers respectively. Moreover, hepatic fibrosis rat model was used for the correlation analysis between the measured liver elasticity and its corresponding pathological changes. Results: The severity of NAFLD in patients was positively correlated with both HA level and FibroScan values suggesting that fibrosis occured along with the progression of NAFLD. Interestingly, the FibroScan value was found to change at an earlier stage of the disease than that of HA level. The mean liver elasticity in the sampling volume of the region of interest in experimental rats, which were subject to the induction of liver fibrosis using peritoneal injection of carbon tetrachloride (CCl4) for 6 or 9 weeks respectively, was measured using Fibroscan. The examination of pathological changes of these experimental rats demonstrated the positive correlation of the measured elasticity values with the extent (severity) of the corresponding pathological changes in the rats of 6 weeks post induction of liver fibrosis with a moderate liver fibrosis, or 9 weeks with a severe liver fibrosis. The average liver stiffness for the normal liver (S0) was 4.51 ± 0.82 kPa, for moderate liver fibrosis (S2) was 6.91 ± 1.32 kPa, and for severe liver fibrosis (S3) 9.62 ± 1.82 kPa. Conclusion: FibroScan can be used to objectively and quantitatively identify the trend in the changing stiffness of the liver and noninvasively detect the development of liver fibrosis.展开更多
Senescence is the last developmental process in plant,which has an important impact on crop yield and quality.In this study,a stable hereditary early-senescence line BC64 was isolated from the high-generation recombin...Senescence is the last developmental process in plant,which has an important impact on crop yield and quality.In this study,a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7(W7).Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11.By linkage analysis,the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4,described as ES(4).Through multiple backcrossing with W7,the near-isogenic line of ES(4)(NIL-ES(4))was obtained.Compared with wild-type W7,NIL-ES(4)plants showed more sever senescence phenotype in both nature and dark conditions.In NIL plants,leaves turned yellow at the fully tillering stage;photosynthetic rate,pollen fertility and seed setting rate were decreased.Moreover,the malondialdehyde,proline content and relative conductivity in NIL-ES(4)were significantly higher than those in W7;both transcript level and activities of reactive oxygen species scavenging enzymes were repressed;H2O2 and O^(2−)were significantly accumulated.This study provides a basis for further cloning and function identification of ES(4).展开更多
文摘Aim: The clinical value of FibroScan in evaluation of hepatic fibrosis was assessed in Chinese patients with nonalcoholic fatty liver disease (NAFLD). Methods: Liver stiffness and other NAFLD related variables were measured in NAFLD patients with either elevated or normal hyaluronic acid (HA) levels, and in healthy volunteers respectively. Moreover, hepatic fibrosis rat model was used for the correlation analysis between the measured liver elasticity and its corresponding pathological changes. Results: The severity of NAFLD in patients was positively correlated with both HA level and FibroScan values suggesting that fibrosis occured along with the progression of NAFLD. Interestingly, the FibroScan value was found to change at an earlier stage of the disease than that of HA level. The mean liver elasticity in the sampling volume of the region of interest in experimental rats, which were subject to the induction of liver fibrosis using peritoneal injection of carbon tetrachloride (CCl4) for 6 or 9 weeks respectively, was measured using Fibroscan. The examination of pathological changes of these experimental rats demonstrated the positive correlation of the measured elasticity values with the extent (severity) of the corresponding pathological changes in the rats of 6 weeks post induction of liver fibrosis with a moderate liver fibrosis, or 9 weeks with a severe liver fibrosis. The average liver stiffness for the normal liver (S0) was 4.51 ± 0.82 kPa, for moderate liver fibrosis (S2) was 6.91 ± 1.32 kPa, and for severe liver fibrosis (S3) 9.62 ± 1.82 kPa. Conclusion: FibroScan can be used to objectively and quantitatively identify the trend in the changing stiffness of the liver and noninvasively detect the development of liver fibrosis.
基金supported by grants from the Science Foundation of Jiangxi Province(Grant No.20212ACB215003)the National Natural Science Foundation of China(Grant No.31960403).
文摘Senescence is the last developmental process in plant,which has an important impact on crop yield and quality.In this study,a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7(W7).Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11.By linkage analysis,the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4,described as ES(4).Through multiple backcrossing with W7,the near-isogenic line of ES(4)(NIL-ES(4))was obtained.Compared with wild-type W7,NIL-ES(4)plants showed more sever senescence phenotype in both nature and dark conditions.In NIL plants,leaves turned yellow at the fully tillering stage;photosynthetic rate,pollen fertility and seed setting rate were decreased.Moreover,the malondialdehyde,proline content and relative conductivity in NIL-ES(4)were significantly higher than those in W7;both transcript level and activities of reactive oxygen species scavenging enzymes were repressed;H2O2 and O^(2−)were significantly accumulated.This study provides a basis for further cloning and function identification of ES(4).
