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TFFl甲基化和甲基转移酶SETDB1表达在肺腺癌中的相关性研究
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作者 郭超 韩晓丽 +3 位作者 黄景涛 杨悦 孙光蕊 梁宗英 《中国肿瘤临床》 北大核心 2025年第1期7-11,共5页
目的:探究SET结构域分支型1(SET domain bifurcated 1,SETDB1)表达与三叶因子1(trefoil factor 1,TFF1)基因甲基化的相关性及临床意义。方法:选取于河北省承德医学院附属医院就诊的55例肺腺癌患者的肺腺癌及远癌组织,焦磷酸测序法检测T... 目的:探究SET结构域分支型1(SET domain bifurcated 1,SETDB1)表达与三叶因子1(trefoil factor 1,TFF1)基因甲基化的相关性及临床意义。方法:选取于河北省承德医学院附属医院就诊的55例肺腺癌患者的肺腺癌及远癌组织,焦磷酸测序法检测TFF1基因甲基化水平,实时荧光定量聚合酶链反应(qRT-PCR)检测TFF1 mRNA相对表达,免疫组织化学方法检测TFF1和SETDB1蛋白表达。统计分析TFF1甲基化水平与SETDB1的相关性及临床意义。采用脂质体介导法将SETDB1 siRNA和阴性对照siRNA转染至肺腺癌A549细胞,应用qRT-PCR和Wesrern blot检测SETDB1 mRNA和蛋白表达水平;采用焦磷酸测序检测各组TFF1基因甲基化水平。结果:肺腺癌组织和远癌组织中TFF1基因甲基化率分别为(70.16±6.32)%和(12.48±2.22)%;TFF1 mRNA相对表达量分别为0.56±0.17和1.56±0.22,差异均具有统计学意义(均P<0.05)。免疫组织化学结果显示,TFF1蛋白阳性表达率分别为29.09%(16/55)和65.45%(36/55);SETDB1蛋白阳性表达率分别为74.55%(41/55)和23.64%(13/55),差异均有统计学意义(均P<0.05)。Western blot结果显示SETDB1在癌组织中表达为(72.89±5.27)%,高于远癌组织(24.27±2.37)%;TFF1在癌组织中表达为(15.38±2.33)%,低于远癌组织(72.72±4.48)%,差异均具有统计学意义(均P<0.05)。肺腺癌组织中TFF1甲基化和SETDB1表达具有相关性(r=0.486,P<0.05);TFF1甲基化和SETDB1与肿瘤TNM分期、组织分化和淋巴结转移密切相关(P<0.05)。下调SETDB1后,TFF1基因甲基化率上升(P<0.05)。结论:在肺腺癌病变进程中,SETDB1的表达与TFF1基因甲基化水平有相关性,SETDB1高表达可能是TFF1基因甲基化的催化酶。 展开更多
关键词 肺腺癌 TFF1 基因 甲基化 SETDB1 表观遗传学
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Intermolecular[4+2]process of N-acyliminium ions with simple olefins for construction of functional substituted-1,3-oxazinan-2-ones
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作者 xiaoli han Xiaodi Nie +3 位作者 Yiman Feng Bangguo Wei Changmei Si Guoqiang Lin 《Chinese Chemical Letters》 SCIE CAS CSCD 2021年第11期3526-3530,共5页
An efficient approach to functionalized 4,6-disubstituted-and 4,6,6-trisubstituted-1,3-oxazinan-2-ones skeleton has been developed through the reaction of semicyclic N,O-acetals 4a and 4b with 1,1-disubstituted ethyle... An efficient approach to functionalized 4,6-disubstituted-and 4,6,6-trisubstituted-1,3-oxazinan-2-ones skeleton has been developed through the reaction of semicyclic N,O-acetals 4a and 4b with 1,1-disubstituted ethylenes 5 or 8.As a result of such a[4+2]cycloaddition process,4,6,6-trisubstituted-1,3-oxazinan-2-ones 6aa,6af-6au,7ba,7bf-7bw and 6,6-spiro containing 1,3-oxazinan-2-ones 9ad,9ae,10ba-10bg were obtained in 36%-96%yields and with moderate to excellent diastereoselectivities.In addition,the synthesis of(±)-norallosedamine 12 could be conveniently achieved from the cycloadduct 7bf. 展开更多
关键词 N-acyliminium ions Simple olefins Intermolecular[4+2]process Substituted-1 3-oxazinan-2-ones Spiro
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Lorentz调和映射及其热流
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作者 韩小利 刘磊 赵亮 《中国科学:数学》 CSCD 北大核心 2018年第10期1303-1314,共12页
本文系统地介绍了与映到Lorentz流形的调和映射相关的背景知识,并且介绍了与其相关的最新研究进展,主要包括同伦类中的存在性、热流的整体弱解和爆破分析等结果.
关键词 调和映射 LORENTZ流形 热流
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The Second Type Singularities of Symplectic and Lagrangian Mean Curvature Flows 被引量:2
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作者 xiaoli han Jiayu LI Jun SUN 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2011年第2期223-240,共18页
This paper mainly deals with the type II singularities of the mean curvature flow from a symplectic surface or from an almost calibrated Lagrangian surface in a K¨ahler surface.The relation between the maximum of... This paper mainly deals with the type II singularities of the mean curvature flow from a symplectic surface or from an almost calibrated Lagrangian surface in a K¨ahler surface.The relation between the maximum of the Kahler angle and the maximum of |H|2 on the limit flow is studied.The authors also show the nonexistence of type II blow-up flow of a symplectic mean curvature flow which is normal flat or of an almost calibrated Lagrangian mean curvature flow which is flat. 展开更多
关键词 Symplectic surface Lagrangian surface Mean curvature flow
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The Second Variation of the Functional L of Symplectic Critical Surfaces in Kähler Surfaces 被引量:2
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作者 xiaoli han Jiayu Li 《Communications in Mathematics and Statistics》 SCIE 2014年第3期311-330,共20页
Let M be a complete Kähler surface andbe a symplectic surface which is smoothly immersed in M.Letαbe the Kähler angle ofin M.In the previous paper Han and Li(JEMS 12:505–527,2010)2010,we study the symple... Let M be a complete Kähler surface andbe a symplectic surface which is smoothly immersed in M.Letαbe the Kähler angle ofin M.In the previous paper Han and Li(JEMS 12:505–527,2010)2010,we study the symplectic critical surfaces,which are critical points of the functional L=1 cosαdμin the class of symplectic surfaces.In this paper,we calculate the second variation of the functional L and derive some consequences.In particular,we show that,if the scalar curvature of M is positive,is a stable symplectic critical surface with cosα≥δ>0,whose normal bundle admits a holomorphic section X∈L2(),thenis holomorphic.We construct symplectic critical surfaces in C2.We also prove a Liouville theorem for symplectic critical surfaces in C2. 展开更多
关键词 Symplectic critical surface Holomorphic curve Kähler surface
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A global weak solution to the Lorentzian harmonic map flow
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作者 xiaoli han Lei Liu Liang Zhao 《Science China Mathematics》 SCIE CSCD 2020年第1期155-166,共12页
We investigate a parabolic-elliptic system which is related to a harmonic map from a compact Riemann surface with a smooth boundary into a Lorentzian manifold with a warped product metric.We prove that there exists a ... We investigate a parabolic-elliptic system which is related to a harmonic map from a compact Riemann surface with a smooth boundary into a Lorentzian manifold with a warped product metric.We prove that there exists a unique global weak solution for this system which is regular except for at most finitely many singular points. 展开更多
关键词 harmonic map heat flow Lorentzian manifold warped product blow up weak solution
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Gradient Flow of the L_(β)-Functional
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作者 xiaoli han Jiayu Li Jun Sun 《Communications in Mathematical Research》 CSCD 2021年第1期113-140,共28页
In this paper,we start to study the gradient flow of the functional L_(β) introduced by Han-Li-Sun in[8].As a first step,we show that if the initial surface is symplectic in a Kähler surface,then the symplectic ... In this paper,we start to study the gradient flow of the functional L_(β) introduced by Han-Li-Sun in[8].As a first step,we show that if the initial surface is symplectic in a Kähler surface,then the symplectic property is preserved along the gradient flow.Then we show that the singularity of the flow is characterized by the maximal norm of the second fundamental form.When β=1,we derive a monotonicity formula for the flow.As applications,we show that the l-tangent cone of the flow consists of the finite flat planes. 展开更多
关键词 β-symplectic critical surfaces gradient flow monotonicity formula tangent cone
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