OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).MET...OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.展开更多
There have already been several interface models for the analyses of thin interfacial layers in bonded materials. To distinguish their corresponding advantages or limitations, a comparative study is carried out, and a...There have already been several interface models for the analyses of thin interfacial layers in bonded materials. To distinguish their corresponding advantages or limitations, a comparative study is carried out, and a new constitutive-based interface model is proposed. Through numerical examinations, the limitations of typical models are clarified. It is found that the new interface model is an efficient and accurate model, by which both the traction and the displacement jumps across the modelled interface with the thickness of zero are allowed, and the stresses within the interracial layer can also be analyzed.展开更多
Background:Asthma is a common chronic inflammatory airway disease and intermittent hypoxia is increasingly recognized as a factor that may impact disease progression.The present study investigated whether intermittent...Background:Asthma is a common chronic inflammatory airway disease and intermittent hypoxia is increasingly recognized as a factor that may impact disease progression.The present study investigated whether intermittent hypoxia(IH)could aggravate asthma by promoting hypoxia-inducible factor-1α(HIF-1α)/nucleotide-binding oligomerization domain(NOD)-like receptor pyrin domain-containing protein 3(NLRP3)/interleukin(IL)-1β-dependent pyroptosis and the inflammatory response and further elucidated the underlying molecular mechanisms involved.Methods:A total of 49 patients diagnosed with severe bronchial asthma and diagnosed by polysomnography were enrolled at Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,between January 2022 and December 2022,and their general data and induced sputum were collected.BEAS-2B cells were treated with IL-13 and subjected to IH.An ovalbumin(OVA)-treated mouse model was also used to assess the effects of chronic intermittent hypoxia(CIH)on asthma.Pyroptosis,the inflammatory response,and related signalling pathways were assessed in vivo and in vitro.Results:In this study,as the apnoea and hypopnea index(AHI)increased,the proportion of patients with uncontrolled asthma increased.The proportions of neutrophils and the levels of IL-6,IL-8,HIF-1αand NLRP3 in induced sputum were related to the AHI.NLRP3-mediated pyroptosis,which could be mediated by the HIF-1αsignalling pathway,was activated in IL-13 plus IH-treated BEAS-2B cells and in the lungs of OVA/CIH mice.HIF-1αdownregulation significantly reduced lung pyroptosis and ameliorated neutrophil inflammation by modulating the NLRP3/IL-1βpathway both in vitro and in vivo.Similarly,pretreatment with LW6,an inhibitor of HIF-1α,effectively blocked the generation of inflammatory cytokines in neutrophils.In addition,administration of the NLRP3 activator nigericin obviously increased lung neutrophil inflammation.Conclusions:Obstructive sleep apnoea–hypopnea syndrome(OSAHS)is a risk factor for asthma exacerbation.IH aggravates neutrophil inflammation in asthma via NLRP3/IL-1β-dependent pyroptosis mediated by the HIF-1αsignalling pathway,which should be considered a potential therapeutic target for the treatment of asthma with OSAHS.展开更多
基金Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission(10411966200)the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau(2009s023)the Shanghai Leading Academic Discipline Project(S30205)
文摘OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
基金supported by the National Natural Science Foundation of China(No.10632040)
文摘There have already been several interface models for the analyses of thin interfacial layers in bonded materials. To distinguish their corresponding advantages or limitations, a comparative study is carried out, and a new constitutive-based interface model is proposed. Through numerical examinations, the limitations of typical models are clarified. It is found that the new interface model is an efficient and accurate model, by which both the traction and the displacement jumps across the modelled interface with the thickness of zero are allowed, and the stresses within the interracial layer can also be analyzed.
基金This work was supported by Noncommunicable Chronic Diseases-National Science and Technology Major Project(Nos.2024ZD0541700,2023ZD0506700)the National Natural Science Foundation of China(No.82270104)+5 种基金Hubei Provincial Natural Science Foundation(Nos.2024AFB050,2025AFB057)the Postdoctor Project of Hubei Province under Grant No.2024HBBHCXB025Health Commission of Hubei Province Scientific Research Project(No.WJ2023Z010)Yunnan Fundamental Research Project(No.202401CF070021)the Natural Science Foundation of Wuhan(No.2024040801020345)Tongji Hospital Fund Cultivation Project(Nos.2024B10,2024B26).
文摘Background:Asthma is a common chronic inflammatory airway disease and intermittent hypoxia is increasingly recognized as a factor that may impact disease progression.The present study investigated whether intermittent hypoxia(IH)could aggravate asthma by promoting hypoxia-inducible factor-1α(HIF-1α)/nucleotide-binding oligomerization domain(NOD)-like receptor pyrin domain-containing protein 3(NLRP3)/interleukin(IL)-1β-dependent pyroptosis and the inflammatory response and further elucidated the underlying molecular mechanisms involved.Methods:A total of 49 patients diagnosed with severe bronchial asthma and diagnosed by polysomnography were enrolled at Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,between January 2022 and December 2022,and their general data and induced sputum were collected.BEAS-2B cells were treated with IL-13 and subjected to IH.An ovalbumin(OVA)-treated mouse model was also used to assess the effects of chronic intermittent hypoxia(CIH)on asthma.Pyroptosis,the inflammatory response,and related signalling pathways were assessed in vivo and in vitro.Results:In this study,as the apnoea and hypopnea index(AHI)increased,the proportion of patients with uncontrolled asthma increased.The proportions of neutrophils and the levels of IL-6,IL-8,HIF-1αand NLRP3 in induced sputum were related to the AHI.NLRP3-mediated pyroptosis,which could be mediated by the HIF-1αsignalling pathway,was activated in IL-13 plus IH-treated BEAS-2B cells and in the lungs of OVA/CIH mice.HIF-1αdownregulation significantly reduced lung pyroptosis and ameliorated neutrophil inflammation by modulating the NLRP3/IL-1βpathway both in vitro and in vivo.Similarly,pretreatment with LW6,an inhibitor of HIF-1α,effectively blocked the generation of inflammatory cytokines in neutrophils.In addition,administration of the NLRP3 activator nigericin obviously increased lung neutrophil inflammation.Conclusions:Obstructive sleep apnoea–hypopnea syndrome(OSAHS)is a risk factor for asthma exacerbation.IH aggravates neutrophil inflammation in asthma via NLRP3/IL-1β-dependent pyroptosis mediated by the HIF-1αsignalling pathway,which should be considered a potential therapeutic target for the treatment of asthma with OSAHS.
