Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared us...Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared using static wet chemical technique,but these face risks of delamination or deactivation induced by blood flow.Inspired by the flow-shear-stress mediated conformation changes of von Willebrand factor,a novel fluid-driven deposition technique was employed to apply polydopamine-heparin coatings within CBPs.Moreover,most FDA-approved CBPs are designed for high-flow-rate CBPs of major organs like the heart and lungs(1000~8000 ml/min).Few are tailored for low-flow-rate perfusion of other organs such as the liver,kidney and brain(<50-300 mL/min).Our approach addresses this gap by developing low-flow-rate CBPs through anti-thrombogenic coat-ings and anti-hemolytic structural optimizations.In this study,we introduced an axial magnetic direct drive motor with our optimized low-flow-rate CBPs,achieving a stable-low-flow-rate rate ranging from 16.3 mL/min(300 rpm)to 121.0 mL/min(2000 rpm).The resulting CBPs system exhibited enhanced flow stability and hemocompatibility in rabbit model experiments,demonstrating significantly lower hemolysis rates and lower thrombus formation risks.These results indicate that the polydopamine-assisted heparin coating provides shortterm stability under dynamic flow,offering a promising strategy for low-flow-rate CBPs,though its long-term durability and clinical translation potential require further validation.展开更多
Selectively electrochemical conversion of CO2 into organic fuel using renewable electricity is one of the most sought-after processes.In this paper,we report the electrochemical reduction of CO2(CO2RR)on the nanoporou...Selectively electrochemical conversion of CO2 into organic fuel using renewable electricity is one of the most sought-after processes.In this paper,we report the electrochemical reduction of CO2(CO2RR)on the nanoporous Ag electrodes made of compacted Ag nanoparticles(AgNPs),which were prepared by one-step reduction in the water phase with or without the surfactant sodium dodecyl sulfate(SDS).The scanning electron microscope(SEM)characterizations show that the compacted Ag electrodes have the nanoporous morphology formed by stacking AgNPs.Compared with the nanoporous Ag electrode without SDS modification(C-AgNPs),the SDS-modified AgNPs electrode(C-AgNPs-SDS)is highly effective in improving selective CO production in a wide range of potentials(-0.69 V--1.19 V,vs.RHE),with a Faradaic efficiency of 92.2% and a current density of -8.23 mA·cm^-2 for CO production at -0.79 V(vs.RHE).C-AgNPs-SDS is also catalytically stable with only less than 7% deactivation after 8 h of continuous electrolysis.展开更多
When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins ...When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins involved in cellular stress are heat shock proteins （HSPs）.展开更多
基金Beijing Natural Science Foundation 7244510(MJ)CHF-brain 2024-1-5041(MJ)+7 种基金CHF-brain 2024-3-2062(ML)Collaborative innovation project by Chinese Institutes for Medical Research CX25XT02(MJ)National Natural Science Foundation of China 82402444(MJ),52531008(ML),82102220(ML),82027802(XJ)Beijing Hospitals Authority Clinical Medicine Development of special funding support(YGLX202527)(MJ)Excellent Youth Fund of Capital Medical University B2305(ML)The Non-profit Central Research Institute Fund of Chinese Academy of Medical 2023-JKCS-09(ML),2023-JKCS-13(MJ)Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support from Yangfan Project YGLX202325(ML)Research Funding on Translational Medicine from Beijing Municipal Science and Technology Commission Z221100007422023(ML)。
文摘Long-lasting stability of the anticoagulant coating on centrifugal blood pumps(CBPs)is of vital importance to ensure the hemocompatibility of the blood circulation system therein.Heparin coatings are often prepared using static wet chemical technique,but these face risks of delamination or deactivation induced by blood flow.Inspired by the flow-shear-stress mediated conformation changes of von Willebrand factor,a novel fluid-driven deposition technique was employed to apply polydopamine-heparin coatings within CBPs.Moreover,most FDA-approved CBPs are designed for high-flow-rate CBPs of major organs like the heart and lungs(1000~8000 ml/min).Few are tailored for low-flow-rate perfusion of other organs such as the liver,kidney and brain(<50-300 mL/min).Our approach addresses this gap by developing low-flow-rate CBPs through anti-thrombogenic coat-ings and anti-hemolytic structural optimizations.In this study,we introduced an axial magnetic direct drive motor with our optimized low-flow-rate CBPs,achieving a stable-low-flow-rate rate ranging from 16.3 mL/min(300 rpm)to 121.0 mL/min(2000 rpm).The resulting CBPs system exhibited enhanced flow stability and hemocompatibility in rabbit model experiments,demonstrating significantly lower hemolysis rates and lower thrombus formation risks.These results indicate that the polydopamine-assisted heparin coating provides shortterm stability under dynamic flow,offering a promising strategy for low-flow-rate CBPs,though its long-term durability and clinical translation potential require further validation.
文摘Selectively electrochemical conversion of CO2 into organic fuel using renewable electricity is one of the most sought-after processes.In this paper,we report the electrochemical reduction of CO2(CO2RR)on the nanoporous Ag electrodes made of compacted Ag nanoparticles(AgNPs),which were prepared by one-step reduction in the water phase with or without the surfactant sodium dodecyl sulfate(SDS).The scanning electron microscope(SEM)characterizations show that the compacted Ag electrodes have the nanoporous morphology formed by stacking AgNPs.Compared with the nanoporous Ag electrode without SDS modification(C-AgNPs),the SDS-modified AgNPs electrode(C-AgNPs-SDS)is highly effective in improving selective CO production in a wide range of potentials(-0.69 V--1.19 V,vs.RHE),with a Faradaic efficiency of 92.2% and a current density of -8.23 mA·cm^-2 for CO production at -0.79 V(vs.RHE).C-AgNPs-SDS is also catalytically stable with only less than 7% deactivation after 8 h of continuous electrolysis.
基金supported by the National Natural Science Foundation of China (31571321, 31171428)the National Key Research and Develepment Program of China (2016YFE0129200)+1 种基金the Institute of the Fundamental Research Funds of Shandong University (2015JC036)the Open Projects of State Key Laboratory of Molecular Oncology (SKL-KF-2017-17)
文摘When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins involved in cellular stress are heat shock proteins （HSPs）.
