Transparent materials utilized as underwater optical windows are highly vulnerable to various forms of pollution or abrasion due to their intrinsic hydrophilic properties.This susceptibility is particularly pronounced...Transparent materials utilized as underwater optical windows are highly vulnerable to various forms of pollution or abrasion due to their intrinsic hydrophilic properties.This susceptibility is particularly pronounced in underwater environments where pollutants can impede the operation of these optical devices,significantly degrading or even compromising their optical properties.The glass catfish,known for its remarkable transparency in water,maintains surface cleanliness and clarity despite exposure to contaminants,impurities abrasion,and hydraulic pressure.Inspired by the glass catfish’s natural attributes,this study introduces a new solution named subaquatic abrasion-resistant and anti-fouling window(SAAW).Utilizing femtosecond laser ablation and electrodeposition,the SAAW is engineered by embedding fine metal bone structures into a transparent substrate and anti-fouling sliding layer,akin to the sturdy bones among catfish’s body.This approach significantly bolsters the window’s abrasion resistance and anti-fouling performance while maintaining high light transmittance.The sliding layer on the SAAW’s surface remarkably reduces the friction of various liquids,which is the reason that SAAW owns the great anti-fouling property.The SAAW demonstrates outstanding optical clarity even after enduring hundreds of sandpaper abrasions,attributing to the fine metal bone structures bearing all external forces and protecting the sliding layer of SAAW.Furthermore,it exhibits exceptional resistance to biological adhesion and underwater pressure.In a green algae environment,the window remains clean with minimal change in transmittance over one month.Moreover,it retains its wettability and anti-fouling properties when subjected to a depth of 30 m of underwater pressure for 30 d.Hence,the SAAW prepared by femtosecond laser ablation and electrodeposition presents a promising strategy for developing stable optical windows in liquid environments.展开更多
Hard scales in scaly fish species ensure the structural and functional integrity of the inner skin and body,even when subjected to various types of external forces.Mucus and oil secreted from the inner layer of the fi...Hard scales in scaly fish species ensure the structural and functional integrity of the inner skin and body,even when subjected to various types of external forces.Mucus and oil secreted from the inner layer of the fish skin to the surface exhibit resistance to a wide range of liquids,maintaining the antifouling properties of the fish skin surface.Inspired by these biological structures,ultra-sturdy and durable scale-armored-sliding surfaces(SASSs)were fabricated in this study using femtosecond laser electrodeposition(FED).In the FED method,a scaly structure is grown from the substrate across a sliding layer to form an SASS.The unique scale-armored structure offers protection against impact and abrasion while maintaining the performance and integrity of the structure.The mechanical sturdiness of the SASS improved by four orders of magnitude compared to that of the conventional antifouling surface.In addition,the SASS exhibited remarkable chemical durability,excellent hydraulic pressure resistance,liquid repellency,and good corrosion resistance based on characterization using various methods.FED enables the preparation of SASS on several materials,including Cu and Al and more.SASS fabricated using FED has great potential for the application of antifouling surfaces in extremely harsh environments.展开更多
Electrochemiluminescence (ECL) has established itself as an excellent transduction technique in biosensing and light-emitting device, while conventional ECL mechanism depending on spontaneous emission of luminophores ...Electrochemiluminescence (ECL) has established itself as an excellent transduction technique in biosensing and light-emitting device, while conventional ECL mechanism depending on spontaneous emission of luminophores lacks reversibility and tunable emission characters, limiting the universality of ECL technique in the fields of fundamental research and clinical applications. Here, we report the first observation of stimulated emission route in ECL and thus establish a reversible tuning ECL microscopy for single-cell imaging. This microscopy uses a focused red-shifted beam to transfer spontaneous ECL into stimulated ECL, which enables selective and reversible tuning of ECL emission from homogeneous solution, single particles, and single cells. After excluding other possible competitive routes, the stimulated ECL emission route is confirmed by a dual-objective system in which the suppressed spontaneous ECL is accompanied by the enhanced stimulated ECL. By incorporating a commercial donut-shaped beam, the sharpness of single-cell matrix adhesion is improved 2 to 3 times compared with the counterpart in confocal ECL mode. The successful establishment of this stimulated emission ECL will greatly advance the development of light-emitting device and super-resolution ECL microscopy.展开更多
Electrochemiluminescence(ECL)is a typical luminescence process triggered by electrochemical reactions.Due to the separated signal types,ECL measurements have some merits of high sensitivity,low background,and simple c...Electrochemiluminescence(ECL)is a typical luminescence process triggered by electrochemical reactions.Due to the separated signal types,ECL measurements have some merits of high sensitivity,low background,and simple configuration.Coupled with a microscopy setup,ECL microscopy(ECLM)has the unique characteristics of ECL and is also furnished with spatiotemporal resolution.Thus,many applications have been created,including nanoscale sensing,ECL mechanism deciphering,transient events of single objects,and ECLM crossover methods.In this review,we will overview the development and basic knowledge of ECL and then profile the setup design of ECLM.Through the understanding of these two parts,we will next probe the diverse applications of ECLM,combining the inter-relation with each other.Finally,the outlook discussing the expectations of further progress of ECLM technology.展开更多
基金supported by the National Science Foundation of China under Grant Nos(Nos.12127806,62175195)the International Joint Research Laboratory for Micro/Nano Manufacturing and Measurement Technologies。
文摘Transparent materials utilized as underwater optical windows are highly vulnerable to various forms of pollution or abrasion due to their intrinsic hydrophilic properties.This susceptibility is particularly pronounced in underwater environments where pollutants can impede the operation of these optical devices,significantly degrading or even compromising their optical properties.The glass catfish,known for its remarkable transparency in water,maintains surface cleanliness and clarity despite exposure to contaminants,impurities abrasion,and hydraulic pressure.Inspired by the glass catfish’s natural attributes,this study introduces a new solution named subaquatic abrasion-resistant and anti-fouling window(SAAW).Utilizing femtosecond laser ablation and electrodeposition,the SAAW is engineered by embedding fine metal bone structures into a transparent substrate and anti-fouling sliding layer,akin to the sturdy bones among catfish’s body.This approach significantly bolsters the window’s abrasion resistance and anti-fouling performance while maintaining high light transmittance.The sliding layer on the SAAW’s surface remarkably reduces the friction of various liquids,which is the reason that SAAW owns the great anti-fouling property.The SAAW demonstrates outstanding optical clarity even after enduring hundreds of sandpaper abrasions,attributing to the fine metal bone structures bearing all external forces and protecting the sliding layer of SAAW.Furthermore,it exhibits exceptional resistance to biological adhesion and underwater pressure.In a green algae environment,the window remains clean with minimal change in transmittance over one month.Moreover,it retains its wettability and anti-fouling properties when subjected to a depth of 30 m of underwater pressure for 30 d.Hence,the SAAW prepared by femtosecond laser ablation and electrodeposition presents a promising strategy for developing stable optical windows in liquid environments.
基金supported by the National Science Foundation of China(12127806 and 62175195)the International Joint Research Laboratory for Micro/Nano Manufacturing and Measurement Technologies.
文摘Hard scales in scaly fish species ensure the structural and functional integrity of the inner skin and body,even when subjected to various types of external forces.Mucus and oil secreted from the inner layer of the fish skin to the surface exhibit resistance to a wide range of liquids,maintaining the antifouling properties of the fish skin surface.Inspired by these biological structures,ultra-sturdy and durable scale-armored-sliding surfaces(SASSs)were fabricated in this study using femtosecond laser electrodeposition(FED).In the FED method,a scaly structure is grown from the substrate across a sliding layer to form an SASS.The unique scale-armored structure offers protection against impact and abrasion while maintaining the performance and integrity of the structure.The mechanical sturdiness of the SASS improved by four orders of magnitude compared to that of the conventional antifouling surface.In addition,the SASS exhibited remarkable chemical durability,excellent hydraulic pressure resistance,liquid repellency,and good corrosion resistance based on characterization using various methods.FED enables the preparation of SASS on several materials,including Cu and Al and more.SASS fabricated using FED has great potential for the application of antifouling surfaces in extremely harsh environments.
基金the National Natural Science Foundation of China(grant numbers 22374125,21834004,22174061,22076161,22176086,and 22025403)the Natural Science Foundation of Jiangsu Province(grant number BK20210189)+4 种基金the State Key Laboratory of Pollution Control and Resource Reuse(grant number PCRR-ZZ-202106)the Foundation of State Key Laboratory of Analytical Chemistry for Life Science(grant number SKLACLS2201)Yangzhou University Interdisciplinary Research Foundation for Chemistry Discipline of Targeted Support(grant number yzuxk202009)Fund for Jiangsu Distinguished Professor and Yangzhou University Start-up Fund,Lvyangjinfeng Talent Program of Yangzhou,Young academic leaders of Jiangsu Province(2018)Talent Support Program of Yangzhou University,and The Open Project Program of Jiangsu Key Laboratory of Zoonosis(no.R2013).
文摘Electrochemiluminescence (ECL) has established itself as an excellent transduction technique in biosensing and light-emitting device, while conventional ECL mechanism depending on spontaneous emission of luminophores lacks reversibility and tunable emission characters, limiting the universality of ECL technique in the fields of fundamental research and clinical applications. Here, we report the first observation of stimulated emission route in ECL and thus establish a reversible tuning ECL microscopy for single-cell imaging. This microscopy uses a focused red-shifted beam to transfer spontaneous ECL into stimulated ECL, which enables selective and reversible tuning of ECL emission from homogeneous solution, single particles, and single cells. After excluding other possible competitive routes, the stimulated ECL emission route is confirmed by a dual-objective system in which the suppressed spontaneous ECL is accompanied by the enhanced stimulated ECL. By incorporating a commercial donut-shaped beam, the sharpness of single-cell matrix adhesion is improved 2 to 3 times compared with the counterpart in confocal ECL mode. The successful establishment of this stimulated emission ECL will greatly advance the development of light-emitting device and super-resolution ECL microscopy.
基金supported by the National Natural Science Foundation of China(Grant Nos.21834004,22174061,and 21904063)the Natural Science Foundation of Jiangsu Province(Grant No.BK20190279)+1 种基金Yangzhou University Interdisciplinary Research Foundation for Chemistry Discipline of Targeted Support(yzuxk202009)Foundation of State Key Laboratory of Analytical Chemistry for Life Science(Grant No.SKLACLS2201).
文摘Electrochemiluminescence(ECL)is a typical luminescence process triggered by electrochemical reactions.Due to the separated signal types,ECL measurements have some merits of high sensitivity,low background,and simple configuration.Coupled with a microscopy setup,ECL microscopy(ECLM)has the unique characteristics of ECL and is also furnished with spatiotemporal resolution.Thus,many applications have been created,including nanoscale sensing,ECL mechanism deciphering,transient events of single objects,and ECLM crossover methods.In this review,we will overview the development and basic knowledge of ECL and then profile the setup design of ECLM.Through the understanding of these two parts,we will next probe the diverse applications of ECLM,combining the inter-relation with each other.Finally,the outlook discussing the expectations of further progress of ECLM technology.
