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紫花苜蓿低温诱导蛋白MsLTI65的分离及其对不同逆境的响应
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作者 周昕越 王丽萍 +3 位作者 蒋庆雪 马晓冉 仪登霞 王学敏 《草业学报》 北大核心 2025年第5期89-104,共16页
LTI是植物中一类低温诱导蛋白,在植物响应非生物胁迫中发挥着重要的作用。为明确紫花苜蓿LTI蛋白的结构特征及在不同非生物胁迫下的响应,通过RT-PCR和3′/5′RACE PCR技术,成功从“中苜1号”紫花苜蓿中克隆得到MsLTI65基因cDNA序列。利... LTI是植物中一类低温诱导蛋白,在植物响应非生物胁迫中发挥着重要的作用。为明确紫花苜蓿LTI蛋白的结构特征及在不同非生物胁迫下的响应,通过RT-PCR和3′/5′RACE PCR技术,成功从“中苜1号”紫花苜蓿中克隆得到MsLTI65基因cDNA序列。利用生物信息学软件对基因序列和结构进行分析,并与其他植物的LTI蛋白进行系统进化树构建,分析它们之间的进化关系。采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹(western-blot,WB)技术分析MsLTI65在不同非生物胁迫条件下的表达模式。序列分析表明,MsLTI65基因编码区序列长2016bp,编码671个氨基酸,分子量74 kDa,理论等电点为4.55,MsLTI65蛋白与蒺藜苜蓿的MtLTI65蛋白具有较高的同源性。qRT-PCR检测结果表明,MsLTI65基因在冷、盐、干旱、Cu^(2+)、Zn^(2+)和脱落酸(ABA)胁迫下表达量均受到诱导上调表达。抗体制备结果表明,已成功制备MsLTI65多克隆抗体,该多克隆抗体特异性高,能够识别天然LTI65样本。Western-blot验证结果表明,MsLTI65蛋白受冷、干旱、盐、ABA胁迫诱导表达。以上结果表明,MsLTI65基因可能作为一个正向调控因子在冷、干旱、Cu^(2+)、Zn^(2+)、盐等多种非生物胁迫信号和ABA激素信号转导过程中发挥重要作用。 展开更多
关键词 紫花苜蓿 MsLTI65 基因克隆 表达分析 抗体 非生物胁迫
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开窗髓核摘除联合椎间支点植骨术治疗腰椎间盘突出症 被引量:6
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作者 马潇苒 赵庆安 +5 位作者 李无阴 马虎升 李东方 李俊辉 孙彦鹏 彭晓东 《中国现代医学杂志》 CAS 2019年第9期90-93,共4页
目的探讨开窗髓核摘除联合椎间支点植骨术治疗腰椎间盘突出症的临床疗效。方法选取2010年1月—2012年12月河南洛阳正骨医院脊柱外科收治的42例腰椎间盘突出症患者采用开窗髓核摘除联合椎间支点植骨术治疗。疗效评定依据疼痛视觉模拟评分... 目的探讨开窗髓核摘除联合椎间支点植骨术治疗腰椎间盘突出症的临床疗效。方法选取2010年1月—2012年12月河南洛阳正骨医院脊柱外科收治的42例腰椎间盘突出症患者采用开窗髓核摘除联合椎间支点植骨术治疗。疗效评定依据疼痛视觉模拟评分(VAS)和Oswestry功能障碍指数(ODI)。患者术前及术后随访时二维CT片对椎间隙高度、椎间植骨块长度和直径比较,评价手术疗效。用Bridwell方法评价腰椎融合情况。结果 42例患者均获得随访,随访时间12~48个月。术后48个月下肢疼痛VAS及ODI较术前减低,差异有统计学意义(P <0.05)。术后不同时间点椎间隙高度、植骨柱长度和直径较术前比较无差异(P>0.05)。末次随访,根据Bridwell腰椎间融合评价标准,Ⅱ和Ⅲ级37例(88%)。所有患者无永久性神经根损伤、无大血管损伤和硬膜囊撕裂等并发症。结论开窗单纯髓核摘除联合椎间支点植骨术治疗腰椎间盘突出症手术简单、费用低、临床疗效好、而且可保留椎间盘突出节段活动度。 展开更多
关键词 椎间盘移位 骨移植 髓核摘除
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Polarization-dependent ultrafast carrier dynamics in GaAs with anisotropic response
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作者 Ya-Chao Li Chao Ge +5 位作者 Peng Wang Shuang Liu xiao-ran ma Bing Wang Hai-Ying Song Shi-Bing Liu 《Chinese Physics B》 SCIE EI CAS CSCD 2022年第6期635-642,共8页
The transient dynamics of anisotropic properties of Ga As was systematically studied by polarization-dependent ultrafast time-resolved transient absorption.Our findings revealed that the anisotropy of reflectivity was... The transient dynamics of anisotropic properties of Ga As was systematically studied by polarization-dependent ultrafast time-resolved transient absorption.Our findings revealed that the anisotropy of reflectivity was enhanced in both pump-induced and probe-induced processes,suggesting an extraordinary resonance absorption of photon-phonon coupling(PPC)with intrinsic anisotropic characteristic in carrier relaxation,regardless of the concrete crystallinity and orientation of GaAs sample.The results,delivering in-depth cognition about the polarization-dependent ultrafast carrier dynamics,also proved the paramount importance of interaction between polarized laser and semiconductor. 展开更多
关键词 ultrafast pump-probe polarization-dependence photon-phonon coupling GAAS
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Thoughts on the treatment of cancer pain under the guidance of traditional Chinese medicine
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作者 Jing Zhuang xiao-ran ma Xue Wang 《TMR Theory and Hypothesis》 2018年第3期83-88,共6页
Chinese medicine diagnosis and treatment of cancer is a feature of traditional Chinese medicine in the prevention and treatment of major diseases.Cancer pain is one of the main clinical symptoms of cancer patients,esp... Chinese medicine diagnosis and treatment of cancer is a feature of traditional Chinese medicine in the prevention and treatment of major diseases.Cancer pain is one of the main clinical symptoms of cancer patients,especially those with advanced cancer,which seriously affects the quality of life of patients.At present,the principle of three-step analgesic ladder is mainly used in the treatment of cancer pain.However,the side effects such as constipation,nausea and vomiting caused by long-term use of analgesic drugs are often limited by patients'tolerance,meanwhile the pain is not effectively controlled.The mechanism of cancer pain is extremely complex.To fully understand the pathogenesis and syndromes of cancer pain in traditional Chinese,medicine is an important basis for effectively alleviating the symptoms of cancer pain.Chinese medicine has unique advantages in treating cancer pain,such as addiction,drug resistance and side effects.Based on the etiology and pathogenesis of cancer pain,this paper discusses the application of different classifications of traditional Chinese medicine in the treatment of cancer pain. 展开更多
关键词 Cancer pain Traditional Chinese medicine Modern medicine
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