Gastric cancer is one of the most frequent neoplasms and a main cause of death worldwide, especially in China and Japan. Numerous epidemiological, animal and experimental studies support a positive association between...Gastric cancer is one of the most frequent neoplasms and a main cause of death worldwide, especially in China and Japan. Numerous epidemiological, animal and experimental studies support a positive association between chronic Helicobacter pylori(H. pylori) infection and the development of gastric cancer. However, the exact mechanism whereby H. pylori causes gastric carcinogenesis remains unclear. It has been demonstrated that expression of cyclooxygenase-2(COX-2) is elevated in gastric carcinomas and in their precursor lesions. In this review, we present the latest clinical and experimental evidence showing the role of gastrin and COX-2 in H. pylori-infected patients and their possible association with gastric cancer risk.展开更多
AIM： To explore the effect of trichostatin A （TSA） on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS： The effect of TSA on growth inhibition and apoptosis was...AIM： To explore the effect of trichostatin A （TSA） on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS： The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot. RESULTS： TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups （37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line） and control group （0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02）. Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of Gl-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.CONCLUSION： TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.展开更多
It has been demonstrated that pitavastatin can significantly reduce low-density lipoprotein(LDL)cholesterol(LDL-C),but its impact on lipoprotein subfractions and oxidized low-density lipoprotein(oxLDL)has not been det...It has been demonstrated that pitavastatin can significantly reduce low-density lipoprotein(LDL)cholesterol(LDL-C),but its impact on lipoprotein subfractions and oxidized low-density lipoprotein(oxLDL)has not been determined.The aim of the present study was to investigate the potential effects of pitavastatin on subfractions of LDL and high-density lipoprotein(HDL)as well as oxLDL in untreated patients with coronary atherosclerosis(AS).Thirty-six subjects were enrolled in this study.O f them,18 patients with AS were administered pitavastatin 2 mg/day for 8 weeks and 18 healthy subjects without therapy served as controls.The plasma lipid profile,lipoprotein subfractions and circulating oxLDL were determined at baseline and 8 weeks respectively.The results showed that pitavastatin treatment indeed not only decreased LDL-C,total cholesterol(TC),triglycerides(TG)and apolipoprotein B(ApoB)levels,and increased HDL cholesterol(HDL-C),but also reduced the cholesterol concentration of all of the LDL subfractions and the percentage of intermediate and small LDL subfractions.Meanwhile,pitavastatin could decrease plasma oxLDL levels.Furthermore,a more close correlation was found between oxLDL and LDL-C as well as LDL subfractions after pitavastatin treatment.We concluded that a moderate dose of pitavastatin therapy not only decreases LDL-C and oxLDL concentrations but also improves LDL subfractions in patients with AS.展开更多
The influence of cooling rate on microstructural evolution and precipitation behavior in Ti,Ti–Nb and Ti-Mo low-carbon steels during the continuous cooling process was studied by dilatometer method,optical microscopy...The influence of cooling rate on microstructural evolution and precipitation behavior in Ti,Ti–Nb and Ti-Mo low-carbon steels during the continuous cooling process was studied by dilatometer method,optical microscopy,and transmission electron microscopy.The results indicated that austenite transformation temperature decreased with the increasing cooling rate in three steels.The addition of Nb and Mo promoted bainite and martensite transformation and improved the hardenability of steels.In addition,precipitates formed in deformed austenite and ferrite can be observed simultaneously.Deformation in the austenite non-recrystallization zone can introduce a large number of deformation bands,and then,the precipitates preferentially nucleated in these deformation bands.In the following process,randomly distributed precipitates and interphase precipitates will be formed in ferrite.The precipitates formed in deformed austenite obey Kurdjumov-Sachs orientation relationship with the matrix,while the precipitates formed in ferrite obey Baker-Nutting orientation relationship with the matrix.The addition of Nb and Mo in Ti-bearing steels decreased the precipitates size and increased the number density of precipitates and then improved the precipitation hardening.And the effect of Mo addition is more obvious than that of Nb addition.展开更多
Objective: To provide comprehensive data to understand mechanisms of vascular endothelial cell(VEC) response to hypoxia/re-oxygenation. Methods: Human umbilical vein endothelial cells(HUVECs) were employed to construc...Objective: To provide comprehensive data to understand mechanisms of vascular endothelial cell(VEC) response to hypoxia/re-oxygenation. Methods: Human umbilical vein endothelial cells(HUVECs) were employed to construct hypoxia/re-oxygenation-induced VEC transcriptome profiling. Cells incubated under 5% O2, 5% CO2, and 90% N2 for 3 h followed by 95% air and 5% CO2 for 1 h were used in the hypoxia/re-oxygenation group. Those incubated only under 95% air and 5% CO2 were used in the normoxia control group. Results: By using a well-established microarray chip consisting of 58 339 probes, the study identified 372 differentially expressed genes. While part of the genes are known to be VEC hypoxia/re-oxygenation-related, serving as a good control, a large number of genes related to VEC hypoxia/re-oxygenation were identified for the first time. Through bioinformatic analysis of these genes, we identified that multiple pathways were involved in the reaction. Subsequently, we applied real-time polymerase chain reaction(PCR) and western blot techniques to validate the microarray data. It was found that the expression of apoptosis-related proteins, like pleckstrin homology-like domain family A member 1(PHLDA1), was also consistently up-regulated in the hypoxia/re-oxygenation group. STRING analysis found that significantly differentially expressed genes SLC38A3, SLC5A5, Lnc-SLC36 A4-1, and Lnc-PLEKHJ1-1 may have physical or/and functional protein–protein interactions with PHLDA1. Conclusions: The data from this study have built a foundation to develop many hypotheses to further explore the hypoxia/re-oxygenation mechanisms, an area with great clinical significance for multiple diseases.展开更多
Objective To investigate the incidence, imaging and clinical characteristics in elderly patients with coronary artery ectasia （CAE）. Methods A retrospective analysis was conducted on patients with CAE who underwent ...Objective To investigate the incidence, imaging and clinical characteristics in elderly patients with coronary artery ectasia （CAE）. Methods A retrospective analysis was conducted on patients with CAE who underwent coronary angiography between January 2006 and December 2012. According to age, the enrolled patients were divided into two groups （elderly group, age≥ 65 years; non-elderly group, age 〈 65 years）. The clinical feature, imaging characteristics and the 5-year survival rate of the two groups were compared.Results The preva-lence of CAE in elderly patients was 0.33%. Patients in elderly group were found to have significantly higher proportion of female （30.1%vs. 10.1%,P〈 0.001）, three-vessel disease （60.5%vs. 45.2%,P = 0.003） and localized ectasia （55.0%vs. 40.2%,P = 0.003）. In addition, body mass index （20.90 ± 2.71 kg/m2vs. 22.31 ± 2.98 kg/m2,P 〈 0.001） and percentage of current smokers （45.0%vs. 64.6%,P 〈 0.001） were significantly lower in elderly group. Cumulative survival curves demonstrated reduced 5-year cumulative survival at the follow-up in the elderly group compared with the non-elderly group （88.0%vs. 96.0%,P = 0.002）. But the 5-year event free survival rate failed to show a significant difference between the two groups （31.0%vs. 35.0%,P= 0.311）.ConclusionThe prevalence of CAE in elderly patients was 0.33%, which was about 1/3 of the entire numbers of CAE patients. There were significant differences between the elderly and the non-elderly patients with CAE in terms of coronary artery disease risk factors and coronary artery ectatic characteristics. CAE might be asso-ciated with increased mortality risk in the elderly.展开更多
BACKGROUND Obesity is associated with an increased risk of developing Crohn’s disease(CD),higher disease activity,and comparatively worse clinical outcomes.AIM To investigate the role of mesenteric adipose tissue-der...BACKGROUND Obesity is associated with an increased risk of developing Crohn’s disease(CD),higher disease activity,and comparatively worse clinical outcomes.AIM To investigate the role of mesenteric adipose tissue-derived exosomes in the pathogenesis of CD aggravation in obese individuals.METHODS First,we induced colitis in mice initiated on high-fat and normal diets and compared the severity of colitis.We then extracted and identified exosomes from mesenteric adipose tissue and determined the levels of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)in mesenteric adipose tissue-derived exosomes and the colon.Next,we demonstrated an interaction between MALAT1 and the miR-15a-5p/activating transcription factor 6(ATF6)axis.Finally,we explored the effects of mesenteric adipose tissue-derived exosomes extracted from mice fed a high-fat or normal diet on the severity of 2,4,6-trinitrobe-nzenesulfonic acid(TNBS)-induced colitis and ATF6-related endoplasmic reticulum stress pathways.RESULTS High-fat diet was found to aggravate TNBS-induced colitis in mice.The expression of MALAT1 in mesenteric adipose tissue-derived exosomes of high-fat diet-fed mice increased.The increased expression of MALAT1 in colon tissue exacerbated TNBS-induced colitis and activated the ATF6 endoplasmic reticulum stress pathway.This effect was partially reversed by the reduced expression of MALAT1 and overexpression of miR-15a-5p.CONCLUSION Mesenteric adipose tissue-derived exosome-encapsulated long noncoding RNAs MALAT1 targets the colon and aggravates TNBS-induced colitis in obese mice,which may potentially act on the miR-15a-5p/ATF6 axis and activate endoplasmic reticulum stress.展开更多
An improved moving least square meshless method is developed for the numerical solution of the nonlinear improved Boussinesq equation. After the approximation of temporal derivatives, nonlinear systems of discrete alg...An improved moving least square meshless method is developed for the numerical solution of the nonlinear improved Boussinesq equation. After the approximation of temporal derivatives, nonlinear systems of discrete algebraic equations are established and are solved by an iterative algorithm. Convergence of the iterative algorithm is discussed. Shifted and scaled basis functions are incorporated into the method to guarantee convergence and stability of numerical results. Numerical examples are presented to demonstrate the high convergence rate and high computational accuracy of the method.展开更多
目的探索改良传统功法“七字专注功”对注意缺陷多动障碍(attention deficit and hyperactive disorder,ADHD)儿童注意力及感觉统合能力的影响。方法选取上海市7~12岁ADHD儿童23例为研究对象,分为试验组(12例)和对照组(11例)。试验组行...目的探索改良传统功法“七字专注功”对注意缺陷多动障碍(attention deficit and hyperactive disorder,ADHD)儿童注意力及感觉统合能力的影响。方法选取上海市7~12岁ADHD儿童23例为研究对象,分为试验组(12例)和对照组(11例)。试验组行“七字专注功”练习,对照组行常规认知及注意力训练。以小组课形式开展试验,每节课的患儿控制在3~5人以保证试验效果,每周每位患儿开展1节课,时长60 min,试验共8周。所有患儿均在干预前、干预后、干预结束3周后接受SNAP-IV量表、Conners量表和SPM感觉加工量表评估,并比较评估结果。结果干预后,试验组在过度活跃、品行问题、学习问题、社会参与等方面较对照组有明显改善(P<0.05);视觉、听觉、触觉、躯体知觉、平衡和运动等指数得分均显著高于对照组(P<0.05)。结论“七字专注功”在改善ADHD儿童注意力及感觉统合能力方面有显著的成效。展开更多
Busulfan/cyclophosphamide(Bu/Cy) conditioning regimen has been widely used to treat cancer patients,while their effects on major internal organs in females are not fully understood.We treated female mice with Bu/Cy,an...Busulfan/cyclophosphamide(Bu/Cy) conditioning regimen has been widely used to treat cancer patients,while their effects on major internal organs in females are not fully understood.We treated female mice with Bu/Cy,and examined the histopathology of major internal organs on Day 30 after the treatment.The results show that Bu/Cy treatment affected the ovaries most extensively,while it had less effect on the spleen,lungs,and kidneys,and no effect on the heart,liver,stomach,and pancreas.To better understand the effect of Bu/Cy on the ovaries,we counted follicles,and determined the levels of ovarian steroids.The Bu/Cy-treated mice showed a reduction of primordial and primary follicles(P<0.01) on Day 30 and a marked loss of follicles at all developmental stages(P<0.01) on Day 60.Plasma levels of estradiol and progesterone in Bu/Cy-treated mice decreased by 43.9% and 61.4%,respectively.Thus,there was a gradual process of follicle loss and low estradiol in Bu/Cy-treated mice;this is a profile similar to what is found in women with premature ovarian failure(POF).The Bu/Cy-treated mice may serve as a useful animal model to study the dynamics of follicle loss in women undergoing POF.展开更多
La_((1-x))Mg_xNi_(4.25)Al_(0.75)(x = 0.0, 0.1, 0.2, 0.3)alloys for tritium storage were prepared by a method of electromagnetic induction melting. The crystal structure and hydrogen storage performance of the as-cast ...La_((1-x))Mg_xNi_(4.25)Al_(0.75)(x = 0.0, 0.1, 0.2, 0.3)alloys for tritium storage were prepared by a method of electromagnetic induction melting. The crystal structure and hydrogen storage performance of the as-cast alloys were investigated. The results showed that a single phase of La Ni_4Al was in the alloys with x = 0.0 and 0.1 and that LaNi_4Al and second phase of(La,Mg)Ni)_3 and AlNi_3 were in the alloys with x = 0.2 and 0.3. On the other hand, the plateau pressures of P–C isotherms of the alloys were increased with the rise of the x value from 0.2 to 0.3 and the hydrogen storage capacity was obviously degraded simultaneously. It was found that the alloy had faster absorption kinetics as the proportion of Mg increased from 0.1 to 0.3.展开更多
Personal credit scoring is the application of financial risk forecasting. It becomes an even important task as financial institutions have been experiencing serious competition and challenges. In this paper, the techn...Personal credit scoring is the application of financial risk forecasting. It becomes an even important task as financial institutions have been experiencing serious competition and challenges. In this paper, the techniques used for credit scoring are summarized and classified and the new method—ensemble learning model is introduced. This article also discusses some problems in current study. It points out that changing the focus from static credit scoring to dynamic behavioral scoring and maximizing revenue by decreasing the Type I and Type II error are two issues in current study. It also suggested that more complex models cannot always been applied to actual situation. Therefore, how to use the assessment models widely and improve the prediction accuracy is the main task for future research.展开更多
The precipitation behavior of nanometer-sized carbides in ferrite in Nb-V-bearing low-carbon steel was studied by electron microscopy and nanoindentation hardness measurements. The results indicated that interphase pr...The precipitation behavior of nanometer-sized carbides in ferrite in Nb-V-bearing low-carbon steel was studied by electron microscopy and nanoindentation hardness measurements. The results indicated that interphase precipitation and random precipitation could occur simultaneously for the specimen isothermally treated at 700 ℃ for 60 min, while in other specimens, only random precipitation was observed. This phenomenon might be explained by mass balance criterion during the diffusional phase transformation. Nanohardness result indicated that the average hardness of the specimens isothermally held at 600 ℃ for 20 min was 3.87 GPa. For the specimen isothermally holding at 650 ℃ for 20 min, the average hardness was 4.10 GPa and the distribution of the nanohardness was in a narrower range compared with that of the specimen isothermal holding at 600 ℃ for 20 min. These implied that the carbides in the specimens isothermal treated at 650℃ were more uniformly dispersed, and the number density of the carbides was greater than that treated at 600 ℃. Using Ashby-Orowan model, the contribution of precipitation strengthening to yield strength was estimated to be 110 MPa for the specimen isothermally treated at the temperature of 650 ℃ for 20 min.展开更多
Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 eu...Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 euthyroid patients were consecutively enrolled and di- vided into CAD group （n = 3306） and non-CAD group （n = 900）. All patients underwent coronary angiography （CAG）. Gensini score （GS） was used to determine the severity of coronary artery stenosis. Severe CAD was defined as GS 〉 32 and mild CAD was defined as GS 〈 32. Logistic regression analysis and linear regression analysis were conducted to determine the association of FT3 with CAD in patients with different gender and ages. Results Concentration of FT3 was lower in patients with CAD than that in angiography-normal control group （P 〈 0.05）. In addition, concentration of FT3 was lower in severe CAD than that in mild CAD. After adjusting for traditional cardiovascular risk factors and potential confounders, FT3 was negatively correlated with the presence of CAD, but not in the old patients （〉 65 years old）. Mul- tivariable linear regression analysis showed that FT3 was negatively associated with GS in male and young patients with stable CAD, but not in the old patients. Conclusions Low FT3 within normal range was negatively associated with the presence and severity of CAD in young patients, but not in the old ones. Further studies are needed to confirm our findings.展开更多
We theoretically study the transition dynamics of a two-component Bose-Einstein condensate driven by a train of weak δ-shaped laser pulses. We find that the atomic system can experience peculiar resonant transition e...We theoretically study the transition dynamics of a two-component Bose-Einstein condensate driven by a train of weak δ-shaped laser pulses. We find that the atomic system can experience peculiar resonant transition even under weak optical excitations and derive the resonance condition by the perturbation method. Employing this mechanism, we propose a scheme to obtain an atomic ensemble with desired odd/even atom number and also a scheme to prepare a nonclassical state of the many-body system with fixed atom number.展开更多
A novel method for designing a beam-switching antenna with the plane dipole is presented. The antenna is composed of double dipoles placed at the center of an active square structure that is divided in four equal sect...A novel method for designing a beam-switching antenna with the plane dipole is presented. The antenna is composed of double dipoles placed at the center of an active square structure that is divided in four equal sectors by metallic sheets. Metallic patches at the outside of the structure are used to enhance the radiation performance of the antenna. In each step, the diodes in one sector are on, whereas other diodes are off. The sector with off-state diodes defines the direction of the radiation pattern. An antenna model is designed on the substrate of FR4. The proposed antenna operates from 4.8 GHz to 5.5 GHz with gain of 6.3 dBi and F/B （front to back ratio） of 13.2 dBi when the operating frequency is 5.2 GHz. The antenna radiation pattern can be swept in the entire azimuth plane in four steps with a 3 dB beamwidth of 90%. The results reveal that the antenna could be used in the base station of the wireless communication systems.展开更多
AIM： To study the genes responsible for retinitis pigmentosa.METHODS： A total of 15 Chinese families with retinitis pigmentosa, containing 94 sporadically afflicted cases, were recruited. The targeted sequences were...AIM： To study the genes responsible for retinitis pigmentosa.METHODS： A total of 15 Chinese families with retinitis pigmentosa, containing 94 sporadically afflicted cases, were recruited. The targeted sequences were captured using the Target_Eye_365_V3 chip and sequenced using the BGISEQ-500 sequencer, according to the manufacturer＇s instructions. Data were aligned to UCSC Genome Browser build hg19, using the Burroughs Wheeler Aligner MEM algorithm. Local realignment was performed with the Genome Analysis Toolkit（GATK v.3.3.0） Indel Realigner, and variants were called with the Genome Analysis Toolkit Haplotypecaller, without any use of imputation. Variants were filtered against a panel derived from 1000 Genomes Project, 1000 G_ASN, ESP6500, Ex AC and db SNP138. In all members of Family ONE and Family TWO with available DNA samples, the genetic variant was validated using Sanger sequencing.RESULTS： A novel, pathogenic variant of retinitis pigmentosa, c.357_358 del AA（p.Ser119 Serfs X5） was identified in PRPF31 in 2 of 15 autosomal-dominant retinitis pigmentosa（ADRP） families, as well as in one, sporadic case. Sanger sequencing was performed uponprobands, as well as upon other family members. This novel, pathogenic genotype co-segregated with retinitis pigmentosa phenotype in these two families. CONCLUSION： ADRP is a subtype of retinitis pigmentosa, defined by its genotype, which accounts for 20%-40% of the retinitis pigmentosa patients. Our study thus expands the spectrum of PRPF31 mutations known to occur in ADRP, and provides further demonstration of the applicability of the BGISEQ500 sequencer for genomics research.展开更多
Medicated diets,a health regimen that integrates the medicinalproperties of herbs with the nutritional benefits of food,aredesigned to facilitate health maintenance and disease prevention,following the principles of T...Medicated diets,a health regimen that integrates the medicinalproperties of herbs with the nutritional benefits of food,aredesigned to facilitate health maintenance and disease prevention,following the principles of Traditional Chinese Medicine in dietarypreparation.The concept of medicated diets is deeply rooted inhuman civilization.According to Zhou Li Tian Guan,as early asthe Zhou Dynasty(256 BC–11th century),royal physicians weredivided into four distinct specializations,with dietetic physiciansranked foremost.These physicians were responsible for managingthe dietary health of the emperor and empress,highlighting thecritical role of diet in health maintenance,which marks the formative stage of the medicated diet concept.展开更多
Background：The currently available polysomnography （PSG） equipments and operating personnel are facing increasing pressure,such situation may result in the problem that a large number of obstructive sleep apnea （...Background：The currently available polysomnography （PSG） equipments and operating personnel are facing increasing pressure,such situation may result in the problem that a large number of obstructive sleep apnea （OSA） patients cannot receive timely diagnosis and treatment,we sought to develop a nomogram quantifying the risk of OSA for a better decision of using PSG,based on the clinical syndromes and the demographic and anthropometric characteristics.Methods：The nomogram was constructed through an ordinal logistic regression procedure.Predictive accuracy and performance characteristics were assessed with the area under the curve （AUC） of the receiver operating characteristics and calibration plots,respectively.Decision curve analyses were applied to assess the net benefit of the nomogram.Results：Among the 401 patients,73 （18.2%） were diagnosed and grouped as the none OSA （apnea-hypopnea index [AHI] 〈5）,67 （16.7%） the mild OSA （5 ≤ AHI 〈 15）,82 （20.4%） the moderate OSA （15 ≤ AHI 〈 30）,and 179 （44.6%） the severe OSA （AHI ≥ 30）.The multivariable analysis suggested the significant factors were duration of disease,smoking status,difficulty of falling asleep,lack of energy,and waist circumference.A nomogram was created for the prediction of OSA using these clinical parameters and was internally validated using bootstrapping method.The discrimination accuracies of the nomogram for any OSA,moderate-severe OSA,and severe OSA were 83.8%,79.9%,and 80.5%,respectively,which indicated good calibration.Decision curve analysis showed that using nomogram could reduce the unnecessary polysomnography （PSG） by 10% without increasing the false negatives.Conclusions：The established clinical nomogram provides high accuracy in predicting the individual risk of OSA.This tool may help physicians better make decisions on PSG arrangement for the patients referred to sleep centers.展开更多
基金Supported by The National Natural Science Foundation of China,No.81072030 and No.81372659Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Gastric cancer is one of the most frequent neoplasms and a main cause of death worldwide, especially in China and Japan. Numerous epidemiological, animal and experimental studies support a positive association between chronic Helicobacter pylori(H. pylori) infection and the development of gastric cancer. However, the exact mechanism whereby H. pylori causes gastric carcinogenesis remains unclear. It has been demonstrated that expression of cyclooxygenase-2(COX-2) is elevated in gastric carcinomas and in their precursor lesions. In this review, we present the latest clinical and experimental evidence showing the role of gastrin and COX-2 in H. pylori-infected patients and their possible association with gastric cancer risk.
文摘AIM： To explore the effect of trichostatin A （TSA） on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS： The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot. RESULTS： TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups （37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line） and control group （0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02）. Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of Gl-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.CONCLUSION： TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.
基金This work was supported,in part,by Capital Special Foundation of Clinical Application Research(No.Z121107001012015)Capital Health Development Fund(No.201614035)+1 种基金CAMS Major Collaborative Innovation Project(No.2016-I2M-1-011)PUMC Youth Fund(No.3332018200).
文摘It has been demonstrated that pitavastatin can significantly reduce low-density lipoprotein(LDL)cholesterol(LDL-C),but its impact on lipoprotein subfractions and oxidized low-density lipoprotein(oxLDL)has not been determined.The aim of the present study was to investigate the potential effects of pitavastatin on subfractions of LDL and high-density lipoprotein(HDL)as well as oxLDL in untreated patients with coronary atherosclerosis(AS).Thirty-six subjects were enrolled in this study.O f them,18 patients with AS were administered pitavastatin 2 mg/day for 8 weeks and 18 healthy subjects without therapy served as controls.The plasma lipid profile,lipoprotein subfractions and circulating oxLDL were determined at baseline and 8 weeks respectively.The results showed that pitavastatin treatment indeed not only decreased LDL-C,total cholesterol(TC),triglycerides(TG)and apolipoprotein B(ApoB)levels,and increased HDL cholesterol(HDL-C),but also reduced the cholesterol concentration of all of the LDL subfractions and the percentage of intermediate and small LDL subfractions.Meanwhile,pitavastatin could decrease plasma oxLDL levels.Furthermore,a more close correlation was found between oxLDL and LDL-C as well as LDL subfractions after pitavastatin treatment.We concluded that a moderate dose of pitavastatin therapy not only decreases LDL-C and oxLDL concentrations but also improves LDL subfractions in patients with AS.
基金supported by the National Natural Science Foundation of China(Grant No.52004224)the Fundamental Research Funds for the Central Universities(No.D5000200031)China Postdoctoral Science Foundation(2020M683559).
文摘The influence of cooling rate on microstructural evolution and precipitation behavior in Ti,Ti–Nb and Ti-Mo low-carbon steels during the continuous cooling process was studied by dilatometer method,optical microscopy,and transmission electron microscopy.The results indicated that austenite transformation temperature decreased with the increasing cooling rate in three steels.The addition of Nb and Mo promoted bainite and martensite transformation and improved the hardenability of steels.In addition,precipitates formed in deformed austenite and ferrite can be observed simultaneously.Deformation in the austenite non-recrystallization zone can introduce a large number of deformation bands,and then,the precipitates preferentially nucleated in these deformation bands.In the following process,randomly distributed precipitates and interphase precipitates will be formed in ferrite.The precipitates formed in deformed austenite obey Kurdjumov-Sachs orientation relationship with the matrix,while the precipitates formed in ferrite obey Baker-Nutting orientation relationship with the matrix.The addition of Nb and Mo in Ti-bearing steels decreased the precipitates size and increased the number density of precipitates and then improved the precipitation hardening.And the effect of Mo addition is more obvious than that of Nb addition.
基金Project supported by the National Natural Science Foundation of China(Nos.81801572 and 81272075)the Foundation of Key Discipline Construction of Zhejiang Province for Traditional Chinese Medicine(No.2017-XKA36)+5 种基金the Foundation of Key Research Project of Zhejiang Province for Traditional Chinese Medicine(No.2019ZZ014)the Medical and Health Science Foundation of Zhejiang Province(No.2019327552)the Key Research and Development Program of Zhejiang Province(No.2019C03076)the General Research Program of Zhejiang Provincial Department of Medical and Health(No.2013KYA066)the Opening Foundation of State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases(Nos.2018KF02 and 2019KF06)the Program of Education Department of Zhejiang Province(No.Y201738150),China。
文摘Objective: To provide comprehensive data to understand mechanisms of vascular endothelial cell(VEC) response to hypoxia/re-oxygenation. Methods: Human umbilical vein endothelial cells(HUVECs) were employed to construct hypoxia/re-oxygenation-induced VEC transcriptome profiling. Cells incubated under 5% O2, 5% CO2, and 90% N2 for 3 h followed by 95% air and 5% CO2 for 1 h were used in the hypoxia/re-oxygenation group. Those incubated only under 95% air and 5% CO2 were used in the normoxia control group. Results: By using a well-established microarray chip consisting of 58 339 probes, the study identified 372 differentially expressed genes. While part of the genes are known to be VEC hypoxia/re-oxygenation-related, serving as a good control, a large number of genes related to VEC hypoxia/re-oxygenation were identified for the first time. Through bioinformatic analysis of these genes, we identified that multiple pathways were involved in the reaction. Subsequently, we applied real-time polymerase chain reaction(PCR) and western blot techniques to validate the microarray data. It was found that the expression of apoptosis-related proteins, like pleckstrin homology-like domain family A member 1(PHLDA1), was also consistently up-regulated in the hypoxia/re-oxygenation group. STRING analysis found that significantly differentially expressed genes SLC38A3, SLC5A5, Lnc-SLC36 A4-1, and Lnc-PLEKHJ1-1 may have physical or/and functional protein–protein interactions with PHLDA1. Conclusions: The data from this study have built a foundation to develop many hypotheses to further explore the hypoxia/re-oxygenation mechanisms, an area with great clinical significance for multiple diseases.
基金This article is partly supported by National Natural Sci-entific Foundation，Specialized Re-search Fund for the Doctoral Program of Higher Education of China，Fund of Capital Special Founda-tion of Clinical Application Research，Capital Health Development Fund，Bei-jing Natural Science Foundation （7131014） awarded to Dr. Jian-Jun Li
文摘Objective To investigate the incidence, imaging and clinical characteristics in elderly patients with coronary artery ectasia （CAE）. Methods A retrospective analysis was conducted on patients with CAE who underwent coronary angiography between January 2006 and December 2012. According to age, the enrolled patients were divided into two groups （elderly group, age≥ 65 years; non-elderly group, age 〈 65 years）. The clinical feature, imaging characteristics and the 5-year survival rate of the two groups were compared.Results The preva-lence of CAE in elderly patients was 0.33%. Patients in elderly group were found to have significantly higher proportion of female （30.1%vs. 10.1%,P〈 0.001）, three-vessel disease （60.5%vs. 45.2%,P = 0.003） and localized ectasia （55.0%vs. 40.2%,P = 0.003）. In addition, body mass index （20.90 ± 2.71 kg/m2vs. 22.31 ± 2.98 kg/m2,P 〈 0.001） and percentage of current smokers （45.0%vs. 64.6%,P 〈 0.001） were significantly lower in elderly group. Cumulative survival curves demonstrated reduced 5-year cumulative survival at the follow-up in the elderly group compared with the non-elderly group （88.0%vs. 96.0%,P = 0.002）. But the 5-year event free survival rate failed to show a significant difference between the two groups （31.0%vs. 35.0%,P= 0.311）.ConclusionThe prevalence of CAE in elderly patients was 0.33%, which was about 1/3 of the entire numbers of CAE patients. There were significant differences between the elderly and the non-elderly patients with CAE in terms of coronary artery disease risk factors and coronary artery ectatic characteristics. CAE might be asso-ciated with increased mortality risk in the elderly.
基金Supported by the National Natural Science Foundation of China,No.81770574the Natural Science Foundation of Zhejiang Province,No.LZ21H030002 and No.LY21H030005.
文摘BACKGROUND Obesity is associated with an increased risk of developing Crohn’s disease(CD),higher disease activity,and comparatively worse clinical outcomes.AIM To investigate the role of mesenteric adipose tissue-derived exosomes in the pathogenesis of CD aggravation in obese individuals.METHODS First,we induced colitis in mice initiated on high-fat and normal diets and compared the severity of colitis.We then extracted and identified exosomes from mesenteric adipose tissue and determined the levels of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)in mesenteric adipose tissue-derived exosomes and the colon.Next,we demonstrated an interaction between MALAT1 and the miR-15a-5p/activating transcription factor 6(ATF6)axis.Finally,we explored the effects of mesenteric adipose tissue-derived exosomes extracted from mice fed a high-fat or normal diet on the severity of 2,4,6-trinitrobe-nzenesulfonic acid(TNBS)-induced colitis and ATF6-related endoplasmic reticulum stress pathways.RESULTS High-fat diet was found to aggravate TNBS-induced colitis in mice.The expression of MALAT1 in mesenteric adipose tissue-derived exosomes of high-fat diet-fed mice increased.The increased expression of MALAT1 in colon tissue exacerbated TNBS-induced colitis and activated the ATF6 endoplasmic reticulum stress pathway.This effect was partially reversed by the reduced expression of MALAT1 and overexpression of miR-15a-5p.CONCLUSION Mesenteric adipose tissue-derived exosome-encapsulated long noncoding RNAs MALAT1 targets the colon and aggravates TNBS-induced colitis in obese mice,which may potentially act on the miR-15a-5p/ATF6 axis and activate endoplasmic reticulum stress.
基金Project supported by the National Natural Science Foundation of China(Grant No.11971085)the Fund from the Chongqing Municipal Education Commission,China(Grant Nos.KJZD-M201800501 and CXQT19018)the Chongqing Research Program of Basic Research and Frontier Technology,China(Grant No.cstc2018jcyjAX0266)。
文摘An improved moving least square meshless method is developed for the numerical solution of the nonlinear improved Boussinesq equation. After the approximation of temporal derivatives, nonlinear systems of discrete algebraic equations are established and are solved by an iterative algorithm. Convergence of the iterative algorithm is discussed. Shifted and scaled basis functions are incorporated into the method to guarantee convergence and stability of numerical results. Numerical examples are presented to demonstrate the high convergence rate and high computational accuracy of the method.
基金Project(No.2010CB945002) supported by the National Basic Research Program(973) of China
文摘Busulfan/cyclophosphamide(Bu/Cy) conditioning regimen has been widely used to treat cancer patients,while their effects on major internal organs in females are not fully understood.We treated female mice with Bu/Cy,and examined the histopathology of major internal organs on Day 30 after the treatment.The results show that Bu/Cy treatment affected the ovaries most extensively,while it had less effect on the spleen,lungs,and kidneys,and no effect on the heart,liver,stomach,and pancreas.To better understand the effect of Bu/Cy on the ovaries,we counted follicles,and determined the levels of ovarian steroids.The Bu/Cy-treated mice showed a reduction of primordial and primary follicles(P<0.01) on Day 30 and a marked loss of follicles at all developmental stages(P<0.01) on Day 60.Plasma levels of estradiol and progesterone in Bu/Cy-treated mice decreased by 43.9% and 61.4%,respectively.Thus,there was a gradual process of follicle loss and low estradiol in Bu/Cy-treated mice;this is a profile similar to what is found in women with premature ovarian failure(POF).The Bu/Cy-treated mice may serve as a useful animal model to study the dynamics of follicle loss in women undergoing POF.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA02020200)the National Natural Science Foundation of China (No. 11475145)the Yangzhou Foundation for Development of Science and Technology (No. YZ2014041)
文摘La_((1-x))Mg_xNi_(4.25)Al_(0.75)(x = 0.0, 0.1, 0.2, 0.3)alloys for tritium storage were prepared by a method of electromagnetic induction melting. The crystal structure and hydrogen storage performance of the as-cast alloys were investigated. The results showed that a single phase of La Ni_4Al was in the alloys with x = 0.0 and 0.1 and that LaNi_4Al and second phase of(La,Mg)Ni)_3 and AlNi_3 were in the alloys with x = 0.2 and 0.3. On the other hand, the plateau pressures of P–C isotherms of the alloys were increased with the rise of the x value from 0.2 to 0.3 and the hydrogen storage capacity was obviously degraded simultaneously. It was found that the alloy had faster absorption kinetics as the proportion of Mg increased from 0.1 to 0.3.
文摘Personal credit scoring is the application of financial risk forecasting. It becomes an even important task as financial institutions have been experiencing serious competition and challenges. In this paper, the techniques used for credit scoring are summarized and classified and the new method—ensemble learning model is introduced. This article also discusses some problems in current study. It points out that changing the focus from static credit scoring to dynamic behavioral scoring and maximizing revenue by decreasing the Type I and Type II error are two issues in current study. It also suggested that more complex models cannot always been applied to actual situation. Therefore, how to use the assessment models widely and improve the prediction accuracy is the main task for future research.
基金supported financially by National Science Foundation of China(Grant Nos.51234002,51504064,and 51474064)National Key Research and Development Program 2016YFB0300601+1 种基金China Postdoctoral Science Foundation 2016M591443the Fundamental Research Funds for the Central Universities N160704002,N160708001
文摘The precipitation behavior of nanometer-sized carbides in ferrite in Nb-V-bearing low-carbon steel was studied by electron microscopy and nanoindentation hardness measurements. The results indicated that interphase precipitation and random precipitation could occur simultaneously for the specimen isothermally treated at 700 ℃ for 60 min, while in other specimens, only random precipitation was observed. This phenomenon might be explained by mass balance criterion during the diffusional phase transformation. Nanohardness result indicated that the average hardness of the specimens isothermally held at 600 ℃ for 20 min was 3.87 GPa. For the specimen isothermally holding at 650 ℃ for 20 min, the average hardness was 4.10 GPa and the distribution of the nanohardness was in a narrower range compared with that of the specimen isothermal holding at 600 ℃ for 20 min. These implied that the carbides in the specimens isothermal treated at 650℃ were more uniformly dispersed, and the number density of the carbides was greater than that treated at 600 ℃. Using Ashby-Orowan model, the contribution of precipitation strengthening to yield strength was estimated to be 110 MPa for the specimen isothermally treated at the temperature of 650 ℃ for 20 min.
文摘Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 euthyroid patients were consecutively enrolled and di- vided into CAD group （n = 3306） and non-CAD group （n = 900）. All patients underwent coronary angiography （CAG）. Gensini score （GS） was used to determine the severity of coronary artery stenosis. Severe CAD was defined as GS 〉 32 and mild CAD was defined as GS 〈 32. Logistic regression analysis and linear regression analysis were conducted to determine the association of FT3 with CAD in patients with different gender and ages. Results Concentration of FT3 was lower in patients with CAD than that in angiography-normal control group （P 〈 0.05）. In addition, concentration of FT3 was lower in severe CAD than that in mild CAD. After adjusting for traditional cardiovascular risk factors and potential confounders, FT3 was negatively correlated with the presence of CAD, but not in the old patients （〉 65 years old）. Mul- tivariable linear regression analysis showed that FT3 was negatively associated with GS in male and young patients with stable CAD, but not in the old patients. Conclusions Low FT3 within normal range was negatively associated with the presence and severity of CAD in young patients, but not in the old ones. Further studies are needed to confirm our findings.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFA0301504)the National Natural Science Foundation of China(Grant Nos.61835013 and 11774362)
文摘We theoretically study the transition dynamics of a two-component Bose-Einstein condensate driven by a train of weak δ-shaped laser pulses. We find that the atomic system can experience peculiar resonant transition even under weak optical excitations and derive the resonance condition by the perturbation method. Employing this mechanism, we propose a scheme to obtain an atomic ensemble with desired odd/even atom number and also a scheme to prepare a nonclassical state of the many-body system with fixed atom number.
基金supported by the Chongqing Municipal Science and Technology Commission of Natural Science Foundation of China under Grant No.cstcjjA40013
文摘A novel method for designing a beam-switching antenna with the plane dipole is presented. The antenna is composed of double dipoles placed at the center of an active square structure that is divided in four equal sectors by metallic sheets. Metallic patches at the outside of the structure are used to enhance the radiation performance of the antenna. In each step, the diodes in one sector are on, whereas other diodes are off. The sector with off-state diodes defines the direction of the radiation pattern. An antenna model is designed on the substrate of FR4. The proposed antenna operates from 4.8 GHz to 5.5 GHz with gain of 6.3 dBi and F/B （front to back ratio） of 13.2 dBi when the operating frequency is 5.2 GHz. The antenna radiation pattern can be swept in the entire azimuth plane in four steps with a 3 dB beamwidth of 90%. The results reveal that the antenna could be used in the base station of the wireless communication systems.
文摘AIM： To study the genes responsible for retinitis pigmentosa.METHODS： A total of 15 Chinese families with retinitis pigmentosa, containing 94 sporadically afflicted cases, were recruited. The targeted sequences were captured using the Target_Eye_365_V3 chip and sequenced using the BGISEQ-500 sequencer, according to the manufacturer＇s instructions. Data were aligned to UCSC Genome Browser build hg19, using the Burroughs Wheeler Aligner MEM algorithm. Local realignment was performed with the Genome Analysis Toolkit（GATK v.3.3.0） Indel Realigner, and variants were called with the Genome Analysis Toolkit Haplotypecaller, without any use of imputation. Variants were filtered against a panel derived from 1000 Genomes Project, 1000 G_ASN, ESP6500, Ex AC and db SNP138. In all members of Family ONE and Family TWO with available DNA samples, the genetic variant was validated using Sanger sequencing.RESULTS： A novel, pathogenic variant of retinitis pigmentosa, c.357_358 del AA（p.Ser119 Serfs X5） was identified in PRPF31 in 2 of 15 autosomal-dominant retinitis pigmentosa（ADRP） families, as well as in one, sporadic case. Sanger sequencing was performed uponprobands, as well as upon other family members. This novel, pathogenic genotype co-segregated with retinitis pigmentosa phenotype in these two families. CONCLUSION： ADRP is a subtype of retinitis pigmentosa, defined by its genotype, which accounts for 20%-40% of the retinitis pigmentosa patients. Our study thus expands the spectrum of PRPF31 mutations known to occur in ADRP, and provides further demonstration of the applicability of the BGISEQ500 sequencer for genomics research.
基金supported by the National Natural Science Foundation of China(82274053 and 82325049)the Scientific and Technological Innovation Project of China Academy of Chinese MedicalSciences(CI2021A04001)the CACMS Innovation Fund(CI2023E002 and CI2023D001).
文摘Medicated diets,a health regimen that integrates the medicinalproperties of herbs with the nutritional benefits of food,aredesigned to facilitate health maintenance and disease prevention,following the principles of Traditional Chinese Medicine in dietarypreparation.The concept of medicated diets is deeply rooted inhuman civilization.According to Zhou Li Tian Guan,as early asthe Zhou Dynasty(256 BC–11th century),royal physicians weredivided into four distinct specializations,with dietetic physiciansranked foremost.These physicians were responsible for managingthe dietary health of the emperor and empress,highlighting thecritical role of diet in health maintenance,which marks the formative stage of the medicated diet concept.
文摘Background：The currently available polysomnography （PSG） equipments and operating personnel are facing increasing pressure,such situation may result in the problem that a large number of obstructive sleep apnea （OSA） patients cannot receive timely diagnosis and treatment,we sought to develop a nomogram quantifying the risk of OSA for a better decision of using PSG,based on the clinical syndromes and the demographic and anthropometric characteristics.Methods：The nomogram was constructed through an ordinal logistic regression procedure.Predictive accuracy and performance characteristics were assessed with the area under the curve （AUC） of the receiver operating characteristics and calibration plots,respectively.Decision curve analyses were applied to assess the net benefit of the nomogram.Results：Among the 401 patients,73 （18.2%） were diagnosed and grouped as the none OSA （apnea-hypopnea index [AHI] 〈5）,67 （16.7%） the mild OSA （5 ≤ AHI 〈 15）,82 （20.4%） the moderate OSA （15 ≤ AHI 〈 30）,and 179 （44.6%） the severe OSA （AHI ≥ 30）.The multivariable analysis suggested the significant factors were duration of disease,smoking status,difficulty of falling asleep,lack of energy,and waist circumference.A nomogram was created for the prediction of OSA using these clinical parameters and was internally validated using bootstrapping method.The discrimination accuracies of the nomogram for any OSA,moderate-severe OSA,and severe OSA were 83.8%,79.9%,and 80.5%,respectively,which indicated good calibration.Decision curve analysis showed that using nomogram could reduce the unnecessary polysomnography （PSG） by 10% without increasing the false negatives.Conclusions：The established clinical nomogram provides high accuracy in predicting the individual risk of OSA.This tool may help physicians better make decisions on PSG arrangement for the patients referred to sleep centers.
