BACKGROUND Enteric anastomotic(EA)bleeding is a potentially life-threatening surgical complication associated with enteric anastomosis during simultaneous pancreas and kidney transplantation(SPKT).AIM To investigate w...BACKGROUND Enteric anastomotic(EA)bleeding is a potentially life-threatening surgical complication associated with enteric anastomosis during simultaneous pancreas and kidney transplantation(SPKT).AIM To investigate whether suture ligation(SL)for submucosal hemostasis during hand-sewn enteric anastomosis could decrease the morbidity of early EA bleeding in SPKT.METHODS We compared the outcomes of 134 patients classified into SL(n=44)and no SL(NSL)groups(n=90).This study adheres to the declarations of Istanbul and Helsinki and all donors were neither paid nor coerced.RESULTS During the first postoperative week,the EA bleeding rate in the SL group was lower than that in the NSL group(2.27%vs 15.56%;P=0.021);no relationship was found between EA bleeding and donor age,mean pancreatic cold ischemia time,platelet count,prothrombin time international normalized rate,activated partial thromboplastin time,and thrombin time.Anastomotic leakage was observed in one case in the SL group at postoperative day(POD)14 and in one case at POD 16 in the NSL group(P=0.754).No significant difference was found between the two groups in the patient survival,pancreas graft survival,or kidney graft survival.CONCLUSION SL for submucosal hemostasis during hand-sewn enteric anastomosis in SPKT can decrease the morbidity of early EA bleeding without increasing the anastomotic leakage rate.展开更多
The photon Doppler velocimetry(PDV) spectrum is investigated in an attempt to reveal the particle parameters of ejecta from shock-loaded samples in a vacuum. A GPU-accelerated Monte–Carlo algorithm, which considers t...The photon Doppler velocimetry(PDV) spectrum is investigated in an attempt to reveal the particle parameters of ejecta from shock-loaded samples in a vacuum. A GPU-accelerated Monte–Carlo algorithm, which considers the multiplescattering effects of light, is applied to reconstruct the light field of the ejecta and simulate the corresponding PDV spectrum.The influence of the velocity profile, total area mass, and particle size of the ejecta on the simulated spectra is discussed qualitatively. To facilitate a quantitative discussion, a novel theoretical optical model is proposed in which the singlescattering assumption is applied. With this model, the relationships between the particle parameters of ejecta and the peak information of the PDV spectrum are derived, enabling direct extraction of the particle parameters from the PDV spectrum.The values of the ejecta parameters estimated from the experimental spectrum are in good agreement with those measured by a piezoelectric probe.展开更多
Multiple steady solutions and hysteresis phenomenon in the square cavity flows driven by the surface with antisymmetric velocity profile are investigated by numerical simulation and bifurcation analysis.A high order s...Multiple steady solutions and hysteresis phenomenon in the square cavity flows driven by the surface with antisymmetric velocity profile are investigated by numerical simulation and bifurcation analysis.A high order spectral element method with the matrix-free pseudo-arclength technique is used for the steady-state solution and numerical continuation.The complex flow patterns beyond the symmetry-breaking at Re≈320 are presented by a bifurcation diagram for Re<2500.The results of stable symmetric and asymmetric solutions are consistent with those reported in literature,and a new unstable asymmetric branch is obtained besides the stable branches.A novel hysteresis phenomenon is observed in the range of 2208<Re<2262,where two pairs of stable and two pairs of unstable asymmetric steady solutions beyond the stable symmetric state coexist.The vortices near the sidewall appear when the Reynolds number increases,which correspond to the bifurcation of topology structure,but not the bifurcation of Navier-Stokes equations.The hysteresis is proposed to be the result of the combined mechanisms of the competition and coalescence of secondary vortices.展开更多
BACKGROUND Accumulating evidence has shown that adipose tissue-derived mesenchymal stem cells(ADSCs)are an effective therapeutic approach for managing coronavirus disease 2019(COVID-19);however,further elucidation is ...BACKGROUND Accumulating evidence has shown that adipose tissue-derived mesenchymal stem cells(ADSCs)are an effective therapeutic approach for managing coronavirus disease 2019(COVID-19);however,further elucidation is required to determine their underlying immunomodulatory effect on the mRNA expression of T helper cell-related transcription factors(TFs)and cytokine release in peripheral blood mononuclear cells(PBMCs).AIM To investigate the impact of ADSCs on the mRNA expression of TFs and cytokine release in PBMCs from colorectal cancer(CRC)patients with severe COVID-19(CRC^(+)patients).METHODS PBMCs from CRC^(+)patients(PBMCs-C+)and age-matched CRC patients(PBMCs-C)were stimulated and cultured in the presence/absence of ADSCs.The mRNA levels of T-box TF TBX21(T-bet),GATA binding protein 3(GATA-3),RAR-related orphan receptor C(RORC),and forkhead box P3(FoxP3)in the PBMCs were determined by reverse transcriptase-polymerase chain reaction.Culture supernatants were evaluated for levels of interferon gamma(IFN-γ),interleukin 4(IL-4),IL-17A,and transforming growth factor beta 1(TGF-β1)using an enzyme-linked immunosorbent assay.RESULTS Compared with PBMCs-C,PBMCs-C+exhibited higher mRNA levels of T-bet and RORC,and increased levels of IFN-γ and IL-17A.Additionally,a significant decrease in FoxP3 mRNA and TGF-β1,as well as an increase in Tbet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios were observed in PBMCs-C+.Furthermore,ADSCs significantly induced a functional regulatory T cell(Treg)subset,as evidenced by an increase in FoxP3 mRNA and TGF-β1 release levels.This was accompanied by a significant decrease in the mRNA levels of T-bet and RORC,release of IFN-γ and IL-17A,and T-bet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios,compared with the PBMCs-C+alone.CONCLUSION The present in vitro studies showed that ADSCs contributed to the immunosuppressive effects on PBMCs-C+,favoring Treg responses.Thus,ADSC-based cell therapy could be a beneficial approach for patients with severe COVID-19 who fail to respond to conventional therapies.展开更多
BACKGROUND The Coexistence of myeloid and lymphoid malignancies is rare.Myeloid leukemia occurs more frequently as a secondary event in patients receiving chemotherapy agents for lymphoid malignancies.Synchronous diag...BACKGROUND The Coexistence of myeloid and lymphoid malignancies is rare.Myeloid leukemia occurs more frequently as a secondary event in patients receiving chemotherapy agents for lymphoid malignancies.Synchronous diagnoses of diffuse large B-cell lymphoma(DLBCL),acute myeloid leukemia(AML),and untreated lymphoplasmacytic lymphoma/Waldenström macroglobulinemia(LPL/WM)in the same patient have not been reported.Here we report one such case.CASE SUMMARY An 89-year-old man had a chest wall mass histopathologically diagnosed as DLBCL.The bone marrow and peripheral blood contained two groups of cells.One group of cells fulfilled the criteria of AML,and the other revealed the features of small B lymphocytic proliferative disorder,which we considered LPL/WM.Multiple chromosomal or genetic changes were detected in bone marrow mononuclear cells,including ATM deletion,CCND1 amplification,mutations of MYD88(L265P)and TP53,WT1 overexpression,and fusion gene of BIRC2-ARAP1,as well as complex chromosomal abnormalities.The patient refused chemotherapy because of old age and died of pneumonia 1 mo after the final diagnosis.CONCLUSION The coexistence of DLBCL,AML,and untreated LPL/WM in the same patient is extremely rare,which probably results from multiple steps of genetic abnormalities.Asymptomatic LPL/WM might have occurred first,then myelodysplastic syndromerelated AML developed,and finally aggressive DLBCL arose.Therefore,medical staff should pay attention to this rare phenomenon to avoid misdiagnoses.展开更多
Alkaline-earth-like(AEL)atoms with two valence electrons and a nonzero nuclear spin can be excited to Rydberg state for quantum computing.Typical AEL ground states possess no hyperfine splitting,but unfortunately a GH...Alkaline-earth-like(AEL)atoms with two valence electrons and a nonzero nuclear spin can be excited to Rydberg state for quantum computing.Typical AEL ground states possess no hyperfine splitting,but unfortunately a GHz-scale splitting seems necessary for Rydberg excitation.Though strong magnetic fields can induce a GHz-scale splitting,weak fields are desirable to avoid noise in experiments.Here,we provide two solutions to this outstanding challenge with realistic data of well-studied AEL isotopes.In the first theory,the two nuclear spin qubit states∣0〉and∣1〉are excited to Rydberg states∣r〉with detuningΔand 0,respectively,where a MHz-scale detuningΔarises from a weak magnetic field on the order of 1 G.With a proper ratio betweenΔandΩ,the qubit state∣1〉can be fully excited to the Rydberg state while∣0〉remains there.In the second theory,we show that by choosing appropriate intermediate states a two-photon Rydberg excitation can proceed with only one nuclear spin qubit state.The second theory is applicable whatever the magnitude of the magnetic field is.These theories bring a versatile means for quantum computation by combining the broad applicability of Rydberg blockade and the incomparable advantages of nuclear-spin quantum memory in two-electron neutral atoms.展开更多
We present a novel class of Rydberg-mediated nuclear-spin entanglement in divalent atoms with global laser pulses.First,we show a fast nuclear-spin controlled phase gate of an arbitrary phase realizable either with tw...We present a novel class of Rydberg-mediated nuclear-spin entanglement in divalent atoms with global laser pulses.First,we show a fast nuclear-spin controlled phase gate of an arbitrary phase realizable either with two laser pulses when assisted by Stark shifts,or with three pulses.Second,we propose to create an electrons−nuclei-entangled state,which is named a super bell state(SBS)for it mimics a large Bell state incorporating three small Bell states.Third,we show a protocol to create a three-atom electrons-nuclei entangled state which contains the three-body W and Greenberger−Horne−Zeilinger(GHZ)states simultaneously.These protocols possess high intrinsic fidelities,do not require single-site Rydberg addressing,and can be executed with large Rydberg Rabi frequencies in a weak,Gauss-scale magnetic field.The latter two protocols can enable measurement-based preparation of Bell,hyperentangled,and GHZ states,and,specifically,SBS can enable quantum dense coding where one can share three classical bits of information by sending one particle.展开更多
To the Editor:A 52-year-old female patient was admitted to the hospital on November 10,2016,due to "four hours of cardiopulmonary arrest after fiber endoscopy." Twelve years ago,she was admitted for a radica...To the Editor:A 52-year-old female patient was admitted to the hospital on November 10,2016,due to "four hours of cardiopulmonary arrest after fiber endoscopy." Twelve years ago,she was admitted for a radical operation of esophageal cancer and underwent radiotherapy and chemotherapy in our hospital.She had no history of respiratory disease,hemoptysis,or drug allergy.Two years ago,the patient underwent a follow-up fiber endoscopy in our hospital.Lidocaine was applied to the throat for surface anesthesia before the endoscopy operation.Propofol,along with oxygen inhalation,was administered intravenously for general anesthesia during the operation.Gastroscopy was performed smoothly,but the patient suddenly became unconscious.Blood spurted out from the airway during tracheal intubation.She was treated with dopamine/metaraminol bitartrate and aminophylline/methylprednisolone.After gaining consciousness,the patient was sent to the Intensive Care Unit.展开更多
基金Supported by National Natural Science Foundation of China,No.81970654.
文摘BACKGROUND Enteric anastomotic(EA)bleeding is a potentially life-threatening surgical complication associated with enteric anastomosis during simultaneous pancreas and kidney transplantation(SPKT).AIM To investigate whether suture ligation(SL)for submucosal hemostasis during hand-sewn enteric anastomosis could decrease the morbidity of early EA bleeding in SPKT.METHODS We compared the outcomes of 134 patients classified into SL(n=44)and no SL(NSL)groups(n=90).This study adheres to the declarations of Istanbul and Helsinki and all donors were neither paid nor coerced.RESULTS During the first postoperative week,the EA bleeding rate in the SL group was lower than that in the NSL group(2.27%vs 15.56%;P=0.021);no relationship was found between EA bleeding and donor age,mean pancreatic cold ischemia time,platelet count,prothrombin time international normalized rate,activated partial thromboplastin time,and thrombin time.Anastomotic leakage was observed in one case in the SL group at postoperative day(POD)14 and in one case at POD 16 in the NSL group(P=0.754).No significant difference was found between the two groups in the patient survival,pancreas graft survival,or kidney graft survival.CONCLUSION SL for submucosal hemostasis during hand-sewn enteric anastomosis in SPKT can decrease the morbidity of early EA bleeding without increasing the anastomotic leakage rate.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11902043 and 11772065)the Science Challenge Project (Grant No. TZ2016001)。
文摘The photon Doppler velocimetry(PDV) spectrum is investigated in an attempt to reveal the particle parameters of ejecta from shock-loaded samples in a vacuum. A GPU-accelerated Monte–Carlo algorithm, which considers the multiplescattering effects of light, is applied to reconstruct the light field of the ejecta and simulate the corresponding PDV spectrum.The influence of the velocity profile, total area mass, and particle size of the ejecta on the simulated spectra is discussed qualitatively. To facilitate a quantitative discussion, a novel theoretical optical model is proposed in which the singlescattering assumption is applied. With this model, the relationships between the particle parameters of ejecta and the peak information of the PDV spectrum are derived, enabling direct extraction of the particle parameters from the PDV spectrum.The values of the ejecta parameters estimated from the experimental spectrum are in good agreement with those measured by a piezoelectric probe.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11902043 and 11772065)the Science Challenge Project(Grant No.TZ2016001).
文摘Multiple steady solutions and hysteresis phenomenon in the square cavity flows driven by the surface with antisymmetric velocity profile are investigated by numerical simulation and bifurcation analysis.A high order spectral element method with the matrix-free pseudo-arclength technique is used for the steady-state solution and numerical continuation.The complex flow patterns beyond the symmetry-breaking at Re≈320 are presented by a bifurcation diagram for Re<2500.The results of stable symmetric and asymmetric solutions are consistent with those reported in literature,and a new unstable asymmetric branch is obtained besides the stable branches.A novel hysteresis phenomenon is observed in the range of 2208<Re<2262,where two pairs of stable and two pairs of unstable asymmetric steady solutions beyond the stable symmetric state coexist.The vortices near the sidewall appear when the Reynolds number increases,which correspond to the bifurcation of topology structure,but not the bifurcation of Navier-Stokes equations.The hysteresis is proposed to be the result of the combined mechanisms of the competition and coalescence of secondary vortices.
基金Supported by National Natural Science Foundation of China,No.81470982.
文摘BACKGROUND Accumulating evidence has shown that adipose tissue-derived mesenchymal stem cells(ADSCs)are an effective therapeutic approach for managing coronavirus disease 2019(COVID-19);however,further elucidation is required to determine their underlying immunomodulatory effect on the mRNA expression of T helper cell-related transcription factors(TFs)and cytokine release in peripheral blood mononuclear cells(PBMCs).AIM To investigate the impact of ADSCs on the mRNA expression of TFs and cytokine release in PBMCs from colorectal cancer(CRC)patients with severe COVID-19(CRC^(+)patients).METHODS PBMCs from CRC^(+)patients(PBMCs-C+)and age-matched CRC patients(PBMCs-C)were stimulated and cultured in the presence/absence of ADSCs.The mRNA levels of T-box TF TBX21(T-bet),GATA binding protein 3(GATA-3),RAR-related orphan receptor C(RORC),and forkhead box P3(FoxP3)in the PBMCs were determined by reverse transcriptase-polymerase chain reaction.Culture supernatants were evaluated for levels of interferon gamma(IFN-γ),interleukin 4(IL-4),IL-17A,and transforming growth factor beta 1(TGF-β1)using an enzyme-linked immunosorbent assay.RESULTS Compared with PBMCs-C,PBMCs-C+exhibited higher mRNA levels of T-bet and RORC,and increased levels of IFN-γ and IL-17A.Additionally,a significant decrease in FoxP3 mRNA and TGF-β1,as well as an increase in Tbet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios were observed in PBMCs-C+.Furthermore,ADSCs significantly induced a functional regulatory T cell(Treg)subset,as evidenced by an increase in FoxP3 mRNA and TGF-β1 release levels.This was accompanied by a significant decrease in the mRNA levels of T-bet and RORC,release of IFN-γ and IL-17A,and T-bet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios,compared with the PBMCs-C+alone.CONCLUSION The present in vitro studies showed that ADSCs contributed to the immunosuppressive effects on PBMCs-C+,favoring Treg responses.Thus,ADSC-based cell therapy could be a beneficial approach for patients with severe COVID-19 who fail to respond to conventional therapies.
基金Supported by the National Natural Science Foundation of China,No.81700130Nanjing Medical University Science and Technology Development Fund.
文摘BACKGROUND The Coexistence of myeloid and lymphoid malignancies is rare.Myeloid leukemia occurs more frequently as a secondary event in patients receiving chemotherapy agents for lymphoid malignancies.Synchronous diagnoses of diffuse large B-cell lymphoma(DLBCL),acute myeloid leukemia(AML),and untreated lymphoplasmacytic lymphoma/Waldenström macroglobulinemia(LPL/WM)in the same patient have not been reported.Here we report one such case.CASE SUMMARY An 89-year-old man had a chest wall mass histopathologically diagnosed as DLBCL.The bone marrow and peripheral blood contained two groups of cells.One group of cells fulfilled the criteria of AML,and the other revealed the features of small B lymphocytic proliferative disorder,which we considered LPL/WM.Multiple chromosomal or genetic changes were detected in bone marrow mononuclear cells,including ATM deletion,CCND1 amplification,mutations of MYD88(L265P)and TP53,WT1 overexpression,and fusion gene of BIRC2-ARAP1,as well as complex chromosomal abnormalities.The patient refused chemotherapy because of old age and died of pneumonia 1 mo after the final diagnosis.CONCLUSION The coexistence of DLBCL,AML,and untreated LPL/WM in the same patient is extremely rare,which probably results from multiple steps of genetic abnormalities.Asymptomatic LPL/WM might have occurred first,then myelodysplastic syndromerelated AML developed,and finally aggressive DLBCL arose.Therefore,medical staff should pay attention to this rare phenomenon to avoid misdiagnoses.
文摘Alkaline-earth-like(AEL)atoms with two valence electrons and a nonzero nuclear spin can be excited to Rydberg state for quantum computing.Typical AEL ground states possess no hyperfine splitting,but unfortunately a GHz-scale splitting seems necessary for Rydberg excitation.Though strong magnetic fields can induce a GHz-scale splitting,weak fields are desirable to avoid noise in experiments.Here,we provide two solutions to this outstanding challenge with realistic data of well-studied AEL isotopes.In the first theory,the two nuclear spin qubit states∣0〉and∣1〉are excited to Rydberg states∣r〉with detuningΔand 0,respectively,where a MHz-scale detuningΔarises from a weak magnetic field on the order of 1 G.With a proper ratio betweenΔandΩ,the qubit state∣1〉can be fully excited to the Rydberg state while∣0〉remains there.In the second theory,we show that by choosing appropriate intermediate states a two-photon Rydberg excitation can proceed with only one nuclear spin qubit state.The second theory is applicable whatever the magnitude of the magnetic field is.These theories bring a versatile means for quantum computation by combining the broad applicability of Rydberg blockade and the incomparable advantages of nuclear-spin quantum memory in two-electron neutral atoms.
基金supported by the National Natural Science Foundation of China under Grant Nos.12074300 and 11805146the Innovation Program for Quantum Science and Technology 2021ZD0302100the Fundamental Research Funds for the Central Universities.
文摘We present a novel class of Rydberg-mediated nuclear-spin entanglement in divalent atoms with global laser pulses.First,we show a fast nuclear-spin controlled phase gate of an arbitrary phase realizable either with two laser pulses when assisted by Stark shifts,or with three pulses.Second,we propose to create an electrons−nuclei-entangled state,which is named a super bell state(SBS)for it mimics a large Bell state incorporating three small Bell states.Third,we show a protocol to create a three-atom electrons-nuclei entangled state which contains the three-body W and Greenberger−Horne−Zeilinger(GHZ)states simultaneously.These protocols possess high intrinsic fidelities,do not require single-site Rydberg addressing,and can be executed with large Rydberg Rabi frequencies in a weak,Gauss-scale magnetic field.The latter two protocols can enable measurement-based preparation of Bell,hyperentangled,and GHZ states,and,specifically,SBS can enable quantum dense coding where one can share three classical bits of information by sending one particle.
文摘To the Editor:A 52-year-old female patient was admitted to the hospital on November 10,2016,due to "four hours of cardiopulmonary arrest after fiber endoscopy." Twelve years ago,she was admitted for a radical operation of esophageal cancer and underwent radiotherapy and chemotherapy in our hospital.She had no history of respiratory disease,hemoptysis,or drug allergy.Two years ago,the patient underwent a follow-up fiber endoscopy in our hospital.Lidocaine was applied to the throat for surface anesthesia before the endoscopy operation.Propofol,along with oxygen inhalation,was administered intravenously for general anesthesia during the operation.Gastroscopy was performed smoothly,but the patient suddenly became unconscious.Blood spurted out from the airway during tracheal intubation.She was treated with dopamine/metaraminol bitartrate and aminophylline/methylprednisolone.After gaining consciousness,the patient was sent to the Intensive Care Unit.
