In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the che...In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the chemical components and potential targets of Xiaoyao Wan.The databases of Malacards and Genecards were used to mine the targets related to depression and anxiety.With the help of Cytoscape3.2.1 software,the“Xiaoyao Wan-targets-diseases”network is constructed.The PPI network is constructed through String database.Common targets are enriched by cluster Profiler software package in R language.161 active components of Xiaoyao Wan were screened,and 247 targets related to Xiaoyao Wan were predicted(excluding repetitive targets).The related targets of depression and anxiety were 379,337 respectively.Through the intersection of Venny diagram,24 targets of Xiaoyao Wan,depression and anxiety were obtained.24 key targets were screened out by constructing the interaction network of Xiaoyao Wan,depression and anxiety.Pathway enrichment analysis showed that Xiaoyao Wan mainly acts on key targets involves D(2)dopamine receptor(DRD2),Interleukin-6(IL6),5-hydroxytryptamine receptor 2A(HTR2A),Interleukin-1 beta(IL1B),5-hydroxytryptamine receptor 3A(HTR3A),Sodium-dependent dopamine transporter(SLC6A3),D(1A)dopamine receptor(DRD1),Interleukin-10(IL10),Sodium-dependent noradrenaline transporterin(SLC6A2)and other key targets in the treatment of depression and anxiety,and participates in important pathway processes such as Serotonergic synapse,Dopaminergic synapse,Neuroactive ligand-receptor interaction and regulation of neurotransmitter levels.It is speculated that Xiaoyao Wan plays a role in the treatment of depression and anxiety by affecting neurotransmitters 5-HT and DA,participating in hypothalamic-pituitary-adrenal(HPA)and interfering in the process of inflammation.展开更多
Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when s...Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when subjected to stress stimuli that cause damage,cells initiate the process of mitophagy,resulting in mitochondrial acidification.Therefore,monitoring changes in mitochondrial p H to comprehend the physiological processes associated with mitophagy is essential.In this study,we developed an asymmetric pentamethine cyanine dye Cy5.5-H-Cy N as a probe for continuous monitoring of mitophagy in living cells.By incorporating an azaindole structure into the dye molecule,a ratiometric fluorescence response was achieved that is specifically responsive to p H variations while preserving its ability to target mitochondria and emit near-infrared fluorescence.Through various methods inducing mitophagy,Cy5.5-H-Cy N was employed to determine mitochondrial p H quantitatively,demonstrating its suitability as an ideal probe for continuous monitoring of mitophagy in living cells.展开更多
Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk...Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk fac-tors that remain insufficiently documented.To fill this knowledge gap,we have compiled a global dataset for its prevalence,drawing upon a collection of literature from the last seven decades obtained from bilingual databases.However,a more interactive and dynamic platform is still needed for both academics and policymakers to improve biosecurity measures,limit disease transmission,and prevent future outbreaks at the global and local levels.Here,we developed an advanced visualization platform to depict the prevalence of Salmonella Pullorum and Gallinarum,espe-cially in China,which is categorized by geographical region and temporal span.The platform offers a user-friendly,efficient,and visually engaging tool to explore the prevalence of pullorum disease and fowl typhoid between 1945 and 2021 in different regions.Additionally,this platform allows users to understand the influence of various fac-tors,such as breed,farm mode,economic usage and even the sex of the primary host,chickens,on the prevalence of this disease.We further provided a detailed overview of individual province within China.In particular,by selecting two different provinces on the interface,users can quickly visualize and grasp the disparities in disease prevalence between the chosen regions.This interactive toolkit enables a dynamic exploration of the patterns and factors con-tributing to the prevalence of Salmonella Pullorum and Gallinarum.This interactive platform is freely available open source at http://139.9.85.208/.展开更多
Objective: Around 50% of new nasopharyngeal carcinoma (NPC) cases come from China. The present study aimed to update the surveillance of NPC survival in southern China, and investigate the survival disparities between...Objective: Around 50% of new nasopharyngeal carcinoma (NPC) cases come from China. The present study aimed to update the surveillance of NPC survival in southern China, and investigate the survival disparities between sexes within this patient population. Methods: Patients diagnosed with primary and invasive NPC between 2000 and 2015 were included in this study. Data on demographics, diagnosis, and follow-up to December 2020 were collected. Patients were stratified by diagnosis period, sex, and age at diagnosis. Survival analysis employed cohort and Life Table methods, Kaplan-Meier curves, log-rank tests, and Cox regression. Results: The study included 32,901 patients, of whom 69.6% were males. The overall 5-year survival rate rose from 69.6% in 2000-2003 to 83.3% in 2013-2015, with a consistent average increase of 3.3% every 3 years. For males, the 5-year survival rate increased from 66.3% to 82.0%, faster than females. Kaplan-Meier curves demonstrated a significantly higher survival rate for females than males, and subgroup analysis confirmed this advantage. The Cox proportional hazards model confirmed the lower mortality risk for females (HR 0.75, 95% CI: 0.71 - 0.78), patients with younger ages at diagnosis, and patients diagnosed in more recent years (All P Conclusions: The 5-year survival rate for NPC patients in southern China has significantly and steadily improved from 2000 to 2015, indicating the improved quality of cancer care in China. The survival advantage of female patients is not limited to younger patients but is also observed in postmenopausal patients, despite the gradual narrowing of the gender gap.展开更多
The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural r...The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural resources of the habitat of giant pandas. Analyzing the natural resource utilization of farmers in communities around the habitat of giant pandas is conducive to formulating more reasonable management policies and protecting the habitat of giant pandas more effectively. Based on the field survey data, this paper classifies the main ways of community farmers using the natural resources of the habitat of giant pandas, counts the quantity of resources used and analyzes their change trends. On this basis, this paper selects indicators, constructs a model and analyzes the main influencing factors affecting the natural resources utilization of community farmers from three aspects: the individual characteristics of household heads, the family characteristics of community farmers and the residence characteristics of community farmers.展开更多
Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the is...Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the issue of polyester plastic pollution.Therefore,we explored the interaction between PETase and the substrate(a dimer of the PET monomer ethylene terephthalate,2PET),using a model of PETase and its substrate.In this study,we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering.These PETase mutants were designed and tested.The enzymatic activities of the R61A,L88F,and I179F mutants,which were obtained with a rapid cell-free screening system,exhibited 1.4 fold,2.1 fold,and 2.5 fold increases,respectively,in comparison with wild-type PETase.The I179F mutant showed the highest activity,with the degradation rate of a PET film reaching 22.5 mg perμmol·L^-1 PETase per day.Thus,this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites,and has further illustrated the potential of biodegradable plastics.展开更多
EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several ...EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several deep photometric surveys and there were about 40 785 EW-type binary systems listed in the international variable star index(VSX)by 2017 March 13.7938 of them were observed with LAMOST by 2016 November 30 and their spectral types were identified.Stellar atmospheric parameters of 5363 EW-type binary stars were determined based on good spectroscopic observations.In the paper,those EWs are cataloged and their properties are analyzed.The distributions of orbital period(P),effective temperature(T),gravitational acceleration(log(g)),metallicity([Fe/H])and radial velocity(RV)are presented for these observed EW-type systems.It is shown that about 80.6% of sample stars have metallicity below zero,indicating that EW-type systems are old stellar populations.This is in agreement with the conclusion that EW binaries are formed from moderately close binaries through angular momentum loss via magnetic braking that takes a few hundred million to a few billion years.The unusually high metallicities of a few percent of EWs may be caused by contamination of material from the evolution of unseen neutron stars or black holes in the systems.The correlations between orbital period and effective temperature,gravitational acceleration and metallicity are presented and their scatters are mainly caused by(i)the presence of third bodies and(ii)sometimes wrongly determined periods.It is shown that some EWs contain evolved component stars and the physical properties of EWs mainly depend on their orbital periods.It is found that extremely short-period EWs may be older than their long-period cousins because they have lower metallicities.This reveals that they have a longer timescale of pre-contact evolution and their formation and evolution are mainly driven by angular momentum loss via magnetic braking.展开更多
The effect of thermal-mechanical processing on the microstructure and mechanical properties of the duplex phase Mg-8Li-3Al-0.4Y alloy was investigated.The as-cast alloy was composed ofα-Mg,β-Li,AlLi,Al2Y and MgAlLi2...The effect of thermal-mechanical processing on the microstructure and mechanical properties of the duplex phase Mg-8Li-3Al-0.4Y alloy was investigated.The as-cast alloy was composed ofα-Mg,β-Li,AlLi,Al2Y and MgAlLi2phases.Annealing of the cold rolled alloy at350°C for60min was considered to be optimum.This caused full static recrystallization and spheroidization.A significantβ-Li loss occurred when the annealing time was increased to90min.The optimized annealing treatment produced the following values of the yield strength,ultimate strength and elongation:148MPa,184MPa and35%,respectively.The texture evolution of theα-phase and theβ-phase changed remarkably during thermal-mechanical processing.展开更多
Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow captu...Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow capture frequency bandwidth are still technical bottlenecks that restrict the commercial application of wave energy converters(WECs).In recent years,using a nonlinear stiffness mechanism(NSM)for passive control has provided a new way to solve these technical bottlenecks.This literature review focuses on the research performed on the use of nonlinear mechanisms in wave energy device utilization,including the conceptual design of a mechanism,hydrodynamic models,dynamic characteristics,response mechanisms,and some examples of experimental verification.Finally,future research directions are discussed and recommended.展开更多
基金Key discipline project of traditional Chinese medicine pharmaceutical engineering of Shanxi provincial administration of traditional Chinese medicine(2017)Discipline innovation team project of Shanxi university of Chinese medicine(No.2019-YL11)。
文摘In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the chemical components and potential targets of Xiaoyao Wan.The databases of Malacards and Genecards were used to mine the targets related to depression and anxiety.With the help of Cytoscape3.2.1 software,the“Xiaoyao Wan-targets-diseases”network is constructed.The PPI network is constructed through String database.Common targets are enriched by cluster Profiler software package in R language.161 active components of Xiaoyao Wan were screened,and 247 targets related to Xiaoyao Wan were predicted(excluding repetitive targets).The related targets of depression and anxiety were 379,337 respectively.Through the intersection of Venny diagram,24 targets of Xiaoyao Wan,depression and anxiety were obtained.24 key targets were screened out by constructing the interaction network of Xiaoyao Wan,depression and anxiety.Pathway enrichment analysis showed that Xiaoyao Wan mainly acts on key targets involves D(2)dopamine receptor(DRD2),Interleukin-6(IL6),5-hydroxytryptamine receptor 2A(HTR2A),Interleukin-1 beta(IL1B),5-hydroxytryptamine receptor 3A(HTR3A),Sodium-dependent dopamine transporter(SLC6A3),D(1A)dopamine receptor(DRD1),Interleukin-10(IL10),Sodium-dependent noradrenaline transporterin(SLC6A2)and other key targets in the treatment of depression and anxiety,and participates in important pathway processes such as Serotonergic synapse,Dopaminergic synapse,Neuroactive ligand-receptor interaction and regulation of neurotransmitter levels.It is speculated that Xiaoyao Wan plays a role in the treatment of depression and anxiety by affecting neurotransmitters 5-HT and DA,participating in hypothalamic-pituitary-adrenal(HPA)and interfering in the process of inflammation.
基金the Key Research and Development Program of Hunan province(2020WK2008)the Talent Scholars of Hunan Province for the Innovation of Science and Technology(2020RC5002)the Natural Science Foundation of Hunan Province(2021JJ31130,2020JJ5903),China.
基金supported by the Fundamental Research Funds for the Central Universities(Nos.DUT23YG137 and DUT22LAB601)Liaoning Binhai Laboratory(No.LBLB-202303)+1 种基金Liaoning Province Science and Technology Joint Fund(Nos.2023JH2/101800039 and 2023JH2/101800037)National Natural Science Foundation of China(Nos.21925802,22090011,and 21878039)。
文摘Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when subjected to stress stimuli that cause damage,cells initiate the process of mitophagy,resulting in mitochondrial acidification.Therefore,monitoring changes in mitochondrial p H to comprehend the physiological processes associated with mitophagy is essential.In this study,we developed an asymmetric pentamethine cyanine dye Cy5.5-H-Cy N as a probe for continuous monitoring of mitophagy in living cells.By incorporating an azaindole structure into the dye molecule,a ratiometric fluorescence response was achieved that is specifically responsive to p H variations while preserving its ability to target mitochondria and emit near-infrared fluorescence.Through various methods inducing mitophagy,Cy5.5-H-Cy N was employed to determine mitochondrial p H quantitatively,demonstrating its suitability as an ideal probe for continuous monitoring of mitophagy in living cells.
基金supported by the National Program on the Key Research Project of China(2022YFC2604201)the Zhejiang Provincial Natural Science Founda-tion of China(LZ24C180002+2 种基金LR19C180001)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0083)the Open Project Program of the Jiangsu Key Laboratory of Zoonosis(R1902).
文摘Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk fac-tors that remain insufficiently documented.To fill this knowledge gap,we have compiled a global dataset for its prevalence,drawing upon a collection of literature from the last seven decades obtained from bilingual databases.However,a more interactive and dynamic platform is still needed for both academics and policymakers to improve biosecurity measures,limit disease transmission,and prevent future outbreaks at the global and local levels.Here,we developed an advanced visualization platform to depict the prevalence of Salmonella Pullorum and Gallinarum,espe-cially in China,which is categorized by geographical region and temporal span.The platform offers a user-friendly,efficient,and visually engaging tool to explore the prevalence of pullorum disease and fowl typhoid between 1945 and 2021 in different regions.Additionally,this platform allows users to understand the influence of various fac-tors,such as breed,farm mode,economic usage and even the sex of the primary host,chickens,on the prevalence of this disease.We further provided a detailed overview of individual province within China.In particular,by selecting two different provinces on the interface,users can quickly visualize and grasp the disparities in disease prevalence between the chosen regions.This interactive toolkit enables a dynamic exploration of the patterns and factors con-tributing to the prevalence of Salmonella Pullorum and Gallinarum.This interactive platform is freely available open source at http://139.9.85.208/.
文摘Objective: Around 50% of new nasopharyngeal carcinoma (NPC) cases come from China. The present study aimed to update the surveillance of NPC survival in southern China, and investigate the survival disparities between sexes within this patient population. Methods: Patients diagnosed with primary and invasive NPC between 2000 and 2015 were included in this study. Data on demographics, diagnosis, and follow-up to December 2020 were collected. Patients were stratified by diagnosis period, sex, and age at diagnosis. Survival analysis employed cohort and Life Table methods, Kaplan-Meier curves, log-rank tests, and Cox regression. Results: The study included 32,901 patients, of whom 69.6% were males. The overall 5-year survival rate rose from 69.6% in 2000-2003 to 83.3% in 2013-2015, with a consistent average increase of 3.3% every 3 years. For males, the 5-year survival rate increased from 66.3% to 82.0%, faster than females. Kaplan-Meier curves demonstrated a significantly higher survival rate for females than males, and subgroup analysis confirmed this advantage. The Cox proportional hazards model confirmed the lower mortality risk for females (HR 0.75, 95% CI: 0.71 - 0.78), patients with younger ages at diagnosis, and patients diagnosed in more recent years (All P Conclusions: The 5-year survival rate for NPC patients in southern China has significantly and steadily improved from 2000 to 2015, indicating the improved quality of cancer care in China. The survival advantage of female patients is not limited to younger patients but is also observed in postmenopausal patients, despite the gradual narrowing of the gender gap.
文摘The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural resources of the habitat of giant pandas. Analyzing the natural resource utilization of farmers in communities around the habitat of giant pandas is conducive to formulating more reasonable management policies and protecting the habitat of giant pandas more effectively. Based on the field survey data, this paper classifies the main ways of community farmers using the natural resources of the habitat of giant pandas, counts the quantity of resources used and analyzes their change trends. On this basis, this paper selects indicators, constructs a model and analyzes the main influencing factors affecting the natural resources utilization of community farmers from three aspects: the individual characteristics of household heads, the family characteristics of community farmers and the residence characteristics of community farmers.
基金National Program on Key Basic Research Project by the Ministry of Science and Technology of China(2014CB745100)the National Natural Science Foundation of China(21676190 and 21621004)the Innovative Talents and Platform Program of Tianjin(16PTGCCX00140 and 16PTSYJC00050).
文摘Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the issue of polyester plastic pollution.Therefore,we explored the interaction between PETase and the substrate(a dimer of the PET monomer ethylene terephthalate,2PET),using a model of PETase and its substrate.In this study,we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering.These PETase mutants were designed and tested.The enzymatic activities of the R61A,L88F,and I179F mutants,which were obtained with a rapid cell-free screening system,exhibited 1.4 fold,2.1 fold,and 2.5 fold increases,respectively,in comparison with wild-type PETase.The I179F mutant showed the highest activity,with the degradation rate of a PET film reaching 22.5 mg perμmol·L^-1 PETase per day.Thus,this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites,and has further illustrated the potential of biodegradable plastics.
基金supported by the National Natural Science Foundation of China (No. 11325315)National Major Scientific Project built by the Chinese Academy of SciencesFunding for the project has been provided by the National Development and Reform Commission
文摘EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several deep photometric surveys and there were about 40 785 EW-type binary systems listed in the international variable star index(VSX)by 2017 March 13.7938 of them were observed with LAMOST by 2016 November 30 and their spectral types were identified.Stellar atmospheric parameters of 5363 EW-type binary stars were determined based on good spectroscopic observations.In the paper,those EWs are cataloged and their properties are analyzed.The distributions of orbital period(P),effective temperature(T),gravitational acceleration(log(g)),metallicity([Fe/H])and radial velocity(RV)are presented for these observed EW-type systems.It is shown that about 80.6% of sample stars have metallicity below zero,indicating that EW-type systems are old stellar populations.This is in agreement with the conclusion that EW binaries are formed from moderately close binaries through angular momentum loss via magnetic braking that takes a few hundred million to a few billion years.The unusually high metallicities of a few percent of EWs may be caused by contamination of material from the evolution of unseen neutron stars or black holes in the systems.The correlations between orbital period and effective temperature,gravitational acceleration and metallicity are presented and their scatters are mainly caused by(i)the presence of third bodies and(ii)sometimes wrongly determined periods.It is shown that some EWs contain evolved component stars and the physical properties of EWs mainly depend on their orbital periods.It is found that extremely short-period EWs may be older than their long-period cousins because they have lower metallicities.This reveals that they have a longer timescale of pre-contact evolution and their formation and evolution are mainly driven by angular momentum loss via magnetic braking.
基金Project(51461018)supported by the National Natural Science Foundation of ChinaProjects(20133BAB20008,20144ACB20013)supported by the Key Program of Natural Science Foundation of Jiangxi Province,ChinaProject(20151BDH80006)supported by the International Science and Technology Cooperation Program of Jiangxi Province,China
文摘The effect of thermal-mechanical processing on the microstructure and mechanical properties of the duplex phase Mg-8Li-3Al-0.4Y alloy was investigated.The as-cast alloy was composed ofα-Mg,β-Li,AlLi,Al2Y and MgAlLi2phases.Annealing of the cold rolled alloy at350°C for60min was considered to be optimum.This caused full static recrystallization and spheroidization.A significantβ-Li loss occurred when the annealing time was increased to90min.The optimized annealing treatment produced the following values of the yield strength,ultimate strength and elongation:148MPa,184MPa and35%,respectively.The texture evolution of theα-phase and theβ-phase changed remarkably during thermal-mechanical processing.
基金the State Key Laboratory of Coastal and Offshore Engineering of China(No.LP2019)the 2020 Research Program of Sanya Yazhou Bay Science and Technology City of China(No.SKJC-2020-01-006)+1 种基金the Hainan Provincial Natural Science Foundation of China(No.520QN290)the High-tech Ship Research Projects Sponsored by MIIT of China(No.[2019]357)。
文摘Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow capture frequency bandwidth are still technical bottlenecks that restrict the commercial application of wave energy converters(WECs).In recent years,using a nonlinear stiffness mechanism(NSM)for passive control has provided a new way to solve these technical bottlenecks.This literature review focuses on the research performed on the use of nonlinear mechanisms in wave energy device utilization,including the conceptual design of a mechanism,hydrodynamic models,dynamic characteristics,response mechanisms,and some examples of experimental verification.Finally,future research directions are discussed and recommended.
