Objective:Patients with colon adenocarcinoma(COAD)who undergo radiation therapy develop radiation enteritis(RE).The predictive value of RE in COAD is yet to be established.Berberine,an active compound derived from the...Objective:Patients with colon adenocarcinoma(COAD)who undergo radiation therapy develop radiation enteritis(RE).The predictive value of RE in COAD is yet to be established.Berberine,an active compound derived from the traditional Chinese medicinal plant,Coptis chinensis,has notable anti-inflammatory properties and offers protection to the intestinal mucosa.This study aimed to evaluate the possible therapeutic effect and mechanism of berberine as a treatment for COAD complicated with RE(COAD&RE).Methods:Relevant genetic features of diverse COAD&RE populations were analyzed using bioinformatics and the Cox proportional hazards regression model.The therapeutic targets of berberine were predicted using network pharmacology and molecular docking.In vivo and in vitro experiments were conducted to validate the core genes identified using molecular docking.Results:RE has a certain impact on the prognosis of COAD and berberine may play an important role in the treatment of COAD&RE.In addition,we identified five core therapeutic targets of berberine by network pharmacology and molecular docking:CCND1,MYC,AR,LEP,and CYP19A1.In vivo experiments showed that berberine increased short-term survival rate,body weight,and intestinal epithelial cell recovery in mice after radiation.In an in vitro study,berberine promoted the proliferation of human intestinal epithelial cells and enhanced the radiosensitivity of HT29 cells after radiation,and the relative mRNA expression levels of CCND1 and MYC closely correlated with these effects.Conclusions:This study predicted the potential therapeutic effects of berberine on COAD&RE and verified the relevant mechanisms,which may provide insights and suggestions for the clinical treatment of COAD&RE.展开更多
Purpose Treatment with immune checkpoint inhibitors(ICIs)improves the prognoses of patients with non-small cell lung cancer(NSCLC)but is ineffective in some patients.The lung immune prognostic index(LIPI)can predict r...Purpose Treatment with immune checkpoint inhibitors(ICIs)improves the prognoses of patients with non-small cell lung cancer(NSCLC)but is ineffective in some patients.The lung immune prognostic index(LIPI)can predict response to ICIs treatment in European patients with NSCLC.This study assessed the correlation of LIPI score with outcomes in Chinese patients with advanced NSCLC receiving ICIs.Methods A total of 305 Chinese patients with advanced NSCLC who received ICIs were ultimately included.LIPI score was determined by a high derived neutrophil-to-lymphocyte ratio(dNLR>3)and elevated lactate dehydrogenase(LDH)and classified as“good”(0),“intermediate”(1),or“poor”(2).The effects of baseline LIPI on overall survival(OS),progression-free survival(PFS),disease control rate(DCR),and overall response rate(ORR)were analyzed.Results The good LIPI group had better OS(26.0 months,P<0.0001)and PFS(10.5 months,P<0.0001)than the other two groups,but the three groups had similar ORR(P=0.222).DCR was 79%,65%,and 47%in the good,intermediate,and poor LIPI groups,respectively(P=0.002).Multivariate analysis indicated that an intermediate LIPI score(HR=1.56,P=0.005)and a poor LIPI score(HR=2.68,P<0.001)were independent predictors of poor OS.The findings were simi-lar for PFS.DCR had a significant negative correlation with the LIPI score(P=0.045).Conclusion Our results confirmed that a good LIPI score was related to prolonged survival and better response to ICIs in Chinese patients with advanced NSCLC.The LIPI score might be useful for selecting patients most likely to benefit from ICIs treatment.展开更多
文摘Objective:Patients with colon adenocarcinoma(COAD)who undergo radiation therapy develop radiation enteritis(RE).The predictive value of RE in COAD is yet to be established.Berberine,an active compound derived from the traditional Chinese medicinal plant,Coptis chinensis,has notable anti-inflammatory properties and offers protection to the intestinal mucosa.This study aimed to evaluate the possible therapeutic effect and mechanism of berberine as a treatment for COAD complicated with RE(COAD&RE).Methods:Relevant genetic features of diverse COAD&RE populations were analyzed using bioinformatics and the Cox proportional hazards regression model.The therapeutic targets of berberine were predicted using network pharmacology and molecular docking.In vivo and in vitro experiments were conducted to validate the core genes identified using molecular docking.Results:RE has a certain impact on the prognosis of COAD and berberine may play an important role in the treatment of COAD&RE.In addition,we identified five core therapeutic targets of berberine by network pharmacology and molecular docking:CCND1,MYC,AR,LEP,and CYP19A1.In vivo experiments showed that berberine increased short-term survival rate,body weight,and intestinal epithelial cell recovery in mice after radiation.In an in vitro study,berberine promoted the proliferation of human intestinal epithelial cells and enhanced the radiosensitivity of HT29 cells after radiation,and the relative mRNA expression levels of CCND1 and MYC closely correlated with these effects.Conclusions:This study predicted the potential therapeutic effects of berberine on COAD&RE and verified the relevant mechanisms,which may provide insights and suggestions for the clinical treatment of COAD&RE.
基金supported by the National Natural Science Foundation of China(grant number 81902910).
文摘Purpose Treatment with immune checkpoint inhibitors(ICIs)improves the prognoses of patients with non-small cell lung cancer(NSCLC)but is ineffective in some patients.The lung immune prognostic index(LIPI)can predict response to ICIs treatment in European patients with NSCLC.This study assessed the correlation of LIPI score with outcomes in Chinese patients with advanced NSCLC receiving ICIs.Methods A total of 305 Chinese patients with advanced NSCLC who received ICIs were ultimately included.LIPI score was determined by a high derived neutrophil-to-lymphocyte ratio(dNLR>3)and elevated lactate dehydrogenase(LDH)and classified as“good”(0),“intermediate”(1),or“poor”(2).The effects of baseline LIPI on overall survival(OS),progression-free survival(PFS),disease control rate(DCR),and overall response rate(ORR)were analyzed.Results The good LIPI group had better OS(26.0 months,P<0.0001)and PFS(10.5 months,P<0.0001)than the other two groups,but the three groups had similar ORR(P=0.222).DCR was 79%,65%,and 47%in the good,intermediate,and poor LIPI groups,respectively(P=0.002).Multivariate analysis indicated that an intermediate LIPI score(HR=1.56,P=0.005)and a poor LIPI score(HR=2.68,P<0.001)were independent predictors of poor OS.The findings were simi-lar for PFS.DCR had a significant negative correlation with the LIPI score(P=0.045).Conclusion Our results confirmed that a good LIPI score was related to prolonged survival and better response to ICIs in Chinese patients with advanced NSCLC.The LIPI score might be useful for selecting patients most likely to benefit from ICIs treatment.
