[Objectives]To establish HPLC fingerprints of wild and cultivated Millettia speciosa Champ.,and identify medicinal materials combined with chemical pattern recognition methods,and provide a reference system for the id...[Objectives]To establish HPLC fingerprints of wild and cultivated Millettia speciosa Champ.,and identify medicinal materials combined with chemical pattern recognition methods,and provide a reference system for the identification and quality control of M.speciosa from different sources.[Methods]20 batches of M.speciosa from different sources were determined by HPLC method,and the similarity analysis and evaluation were performed using the Similarity Evaluation System of Traditional Chinese Medicine Chromatographic Fingerprints(2012 edition).Principal component analysis(PCA)and least partial squares method-discrimination analysis(PLS-DA)were used to conduct chemical pattern recognition research on wild and cultivated M.speciosa.[Results]The HPLC fingerprints of wild and cultivated M.speciosa were established,10 common peaks were calibrated,and the similarity of 20 batches of samples was greater than 0.9;PCA can better classify M.speciosa from different sources into 2 categories,and PLS-DA can completely distinguish between wild and cultivated M.speciosa.[Conclusions]The established M.speciosa fingerprint,combined with chemical pattern recognition methods,can effectively distinguish between wild and cultivated M.speciosa,so it can provide a reference for quality control and evaluation of M.speciosa.展开更多
基金City-level Technology Research and Development Project of Qinzhou Science and Technology Bureau(20198509).
文摘[Objectives]To establish HPLC fingerprints of wild and cultivated Millettia speciosa Champ.,and identify medicinal materials combined with chemical pattern recognition methods,and provide a reference system for the identification and quality control of M.speciosa from different sources.[Methods]20 batches of M.speciosa from different sources were determined by HPLC method,and the similarity analysis and evaluation were performed using the Similarity Evaluation System of Traditional Chinese Medicine Chromatographic Fingerprints(2012 edition).Principal component analysis(PCA)and least partial squares method-discrimination analysis(PLS-DA)were used to conduct chemical pattern recognition research on wild and cultivated M.speciosa.[Results]The HPLC fingerprints of wild and cultivated M.speciosa were established,10 common peaks were calibrated,and the similarity of 20 batches of samples was greater than 0.9;PCA can better classify M.speciosa from different sources into 2 categories,and PLS-DA can completely distinguish between wild and cultivated M.speciosa.[Conclusions]The established M.speciosa fingerprint,combined with chemical pattern recognition methods,can effectively distinguish between wild and cultivated M.speciosa,so it can provide a reference for quality control and evaluation of M.speciosa.
