We present a comprehensive electron momentum spectroscopy study on the electronic structure of trifluorobromomethane.The binding energy spectrum and electron momentum profiles of the entire outer-valence orbitals and ...We present a comprehensive electron momentum spectroscopy study on the electronic structure of trifluorobromomethane.The binding energy spectrum and electron momentum profiles of the entire outer-valence orbitals and the first inner-valence orbital along with several shake-up states were measured by using a high-sensitivity(e,2e)apparatus at an electron impact energy of 1213 eV.Theoretical calculations employing the density functional theory with B3LYP hybrid functional and the symmetry-adapted cluster configuration-interaction method were performed to interpret the experimental results.Important effects of electron correlations in the initial neutral and final ionic states on the electron momentum profiles have been observed.展开更多
The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^...The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^(2+),O_(2)^(2+)O^(+),O^(2+),and their total)and two Coulomb explosion channels(O^(+)+O^(+)and O^(2+)+O^(+))are obtained by putting the data of O^(2+)on the scale of Ar+from O_(2)and Ar gases mixed with a fixed relative flow ratio of 1:1.The experimental errors are assessed by taking uncertainties of various factors into account.The present absolute cross sections are well consistent with the previous data in the overlapped energy range below 1000 eV.展开更多
The semiclassical non-perturbative atomic orbital close-coupling approach has been employed to study the electron capture and excitation processes in He^(2+)-H(1s)and He^(2+)-H(2s)collision systems.In order to ensure ...The semiclassical non-perturbative atomic orbital close-coupling approach has been employed to study the electron capture and excitation processes in He^(2+)-H(1s)and He^(2+)-H(2s)collision systems.In order to ensure the accuracy of our calculated cross sections,a large number of high excited states and pseudostates are included in the expansion basis sets which are centered on the target and projectile,respectively.The total and partial charge transfer and excitation cross sections are obtained for a wide-energy domain ranging from 1 keV/amu to 200 keV/amu.The present calculations are also compared with the results from other theoretical methods.These cross section data are useful for the investigation of astrophysics and laboratory plasma.展开更多
Diabetes mellitus(DM)is a major metabolic disease endangering global health,with diabetic nephropathy(DN)as a primary complication lacking curative therapy.Sporoderm-broken spores of Ganoderma lucidum(GLP),an herbal m...Diabetes mellitus(DM)is a major metabolic disease endangering global health,with diabetic nephropathy(DN)as a primary complication lacking curative therapy.Sporoderm-broken spores of Ganoderma lucidum(GLP),an herbal medicine,has been used for the treatment of metabolic disorders.In this study,DN was induced in Sprague-Dawley rats using streptozotocin(STZ)and a high-fat diet(HFD),and the protective mechanisms of GLP were investigated through transcriptomic,metabolomic,and network pharmacology(NP)analyses.Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats.Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes.GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression(Igfbp1 and Angptl4)and enhanced metabolic biomarkers of 4-Aminocatechol.In addition,NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways.In conclusion,this study provides supportive evidence of the anti-inflammatory effects of GLP supplements,highlighting their potential for promising clinical applications in treating DN.展开更多
Studies show that encoding technologies in H.264/AVC,including prediction and conversion,are essential technologies.However,these technologies are more complicated than the MPEG-4,which is a standard method and widely...Studies show that encoding technologies in H.264/AVC,including prediction and conversion,are essential technologies.However,these technologies are more complicated than the MPEG-4,which is a standard method and widely adopted worldwide.Therefore,the amount of calculation in H.264/AVC is significantly up-regulated compared to that of the MPEG-4.In the present study,it is intended to simplify the computational expenses in the international standard compression coding system H.264/AVC for moving images.Inter prediction refers to the most feasible compression technology,taking up to 60%of the entire encoding.In this regard,prediction error and motion vector information are proposed to simplify the computation of inter predictive coding technology.In the initial frame,motion compensation is performed in all target modes and then basic information is collected and analyzed.After the initial frame,motion compensation is performed only in the middle 8×8 modes,and the basic information amount shifts.In order to evaluate the effectiveness of the proposed method and assess the motion image compression coding,four types of motion images,defined by the international telecommunication union(ITU),are employed.Based on the obtained results,it is concluded that the developed method is capable of simplifying the calculation,while it is slightly affected by the inferior image quality and the amount of information.展开更多
Objective： The aim of this study was to evaluate the value of the application of anterolateral thigh flap in postoperative repairing of vulva tumor. Methods： Anterolateral thigh flap with the descending branch of th...Objective： The aim of this study was to evaluate the value of the application of anterolateral thigh flap in postoperative repairing of vulva tumor. Methods： Anterolateral thigh flap with the descending branch of the lateral circumflex femoral artery and the pedicle of anterolaterat thigh cutaneous nerve was used in primary repairing of the defect from postoperative vulva tumor, the sizes of the flaps ranged from 10 cm ×14 cm-14 cm× 20 cm. Results： All flaps survived, during a follow-up of 2 to 12 months, the appearance of the flaps was satisfactory with sensitive function without local recurrences. Conclusion： Anterelateral thigh flap has fairly long vessel pedicle, wider vessel diameter, and is in the covert area. Anterolateral thigh flap with sensory nerve is the ideal choice for postoperative repairing of vulva tumor.展开更多
The outer valence electron momentum distributions of benzene are reinvestigated with theoretical calculations involving the vibrational effects.The results are compared with recent experimental measurements[Phys.Rev.A...The outer valence electron momentum distributions of benzene are reinvestigated with theoretical calculations involving the vibrational effects.The results are compared with recent experimental measurements[Phys.Rev.A 98042705(2018)].The significant discrepancies between theories and experiments in previous works have now been interpreted quantitatively,indicating that the vibrational motion in benzene molecule has noticeable influence on its electron momentum distributions.展开更多
We report the study on the complete three-body Coulomb explosion(CE)of N_(2)O^(q+)(q=5,6)induced by 56-keV/u Ne8+ion collision with N2O gaseous molecule.Six CE channels for N_(2)O^(5+)and seven for N_(2)O^(6+)are iden...We report the study on the complete three-body Coulomb explosion(CE)of N_(2)O^(q+)(q=5,6)induced by 56-keV/u Ne8+ion collision with N2O gaseous molecule.Six CE channels for N_(2)O^(5+)and seven for N_(2)O^(6+)are identified by measuring three ionic fragments and the charge-changed projectile in quadruple coincidence.Correspondingly the kinetic energy release(KER)and momentum correlation angle(MCA)distributions of three ionic fragments for each of the CE channels are also deduced.Numerical computation is presented to reconstruct the geometric structure of N_(2)O^(q+0prior to dissociation based on the measured KER and MCA.The N–N and N–O bond lengths and the N–N–O bond angles of N_(2)O^(q+)for each of the channels are determined.展开更多
We report theoretical studies of electron impact triple differential cross sections of two bio-molecules,pyrimidine and tetrahydrofurfuryl alcohol,in the coplanar asymmetric kinematic conditions with the impact energy...We report theoretical studies of electron impact triple differential cross sections of two bio-molecules,pyrimidine and tetrahydrofurfuryl alcohol,in the coplanar asymmetric kinematic conditions with the impact energy of 250 eV and ejected electron energy of 20 eV at three scattering angles of-5°,-10°,and-15°.Present multi-center distorted-wave method well describes the experimental data,which was obtained by performing(e,2e)experiment.The calculations show that the secondary electron produced by the primary impact electron is strongly influenced by the molecular ionic multi-center potential,which must be considered when the low energy electron interacts with DNA analogues.展开更多
In this work,electron energy spectroscopic mapping of surface plasmon of Ag nanostructures on highly oriented pyrolytic graphite is reported.Benefitting from the angular dispersive feature of the present scanning prob...In this work,electron energy spectroscopic mapping of surface plasmon of Ag nanostructures on highly oriented pyrolytic graphite is reported.Benefitting from the angular dispersive feature of the present scanning probe electron energy spectrometer,a multi-channel detection mode is developed.By scanning along one direction,the two-dimensional intensity distribution of Ag surface plasmon excitation due to the collision of electron emitted from the tip can be obtained in parallel.The spectroscopic spatial resolution is determined to be around 80 nm.展开更多
Background Retinitis pigmentosa(RP)is a genetically and clinically heterogeneous group of hereditary degenerative disorders affecting approximately one in every 4000 people worldwide.Abnormalities in the retina's ...Background Retinitis pigmentosa(RP)is a genetically and clinically heterogeneous group of hereditary degenerative disorders affecting approximately one in every 4000 people worldwide.Abnormalities in the retina's photoreceptors can cause night blindness or even complete vision loss.Retinitis Pigmentosa 1(RP1),also known as the oxygen-regulated protein-1,is a microtubule-associated protein(MAP)that organizes the outer segment of the photoreceptor.Besides,mutations in the RP1 gene are associated with dominant or recessive form of RP.This study aims to identify the potential pathogenic genes in Chinese RP patients and to elucidate the association relationship between the mutant gene and the phenotypes.Methods Multiple ophthalmic examinations,whole-exome sequencing,sanger sequencing,and in silico analysis were performed to evaluate the clinical features and pathogenic genes in a five-generation Chinese family diagnosed with RP.Results Our findings revealed a novel truncating mutation c.2015_2018del p.(Lys672Argfs∗9)in RP1 that may result in the translation of a protein with deleterious effects on photoreceptors.Therefore,resulting in autosomal dominant retinitis pigmentosa(ADRP).Conclusions This study broaden the range of genetic mutations associated with RP1 in ADRP and make a valuable contribution to the ongoing endeavors aimed at characterizing the molecular aspects of Chinese ADRP.Future studies would pay more attention in determining the characterization of the mutantations in RP1 gene and the relationship between genotype and phenotype in RP patients.展开更多
Protein misfolding and aggregation are crucial pathogenic factors for cataracts,which are the leading cause of visual impairment worldwide.α-crystallin,as a small molecular chaperone,is involved in preventing protein...Protein misfolding and aggregation are crucial pathogenic factors for cataracts,which are the leading cause of visual impairment worldwide.α-crystallin,as a small molecular chaperone,is involved in preventing protein misfolding and maintaining lens transparency.The chaperone activity of α-crystallin depends on its oligomeric state.Our previous work identified a natural compound,celastrol,which could regulate the oligomeric state of αB-crystallin.In this work,based on the UNcle and SEC analysis,we found that celastrol induced𝛼αB-crystallin to form large oligomers.Large oligomer formation enhanced the chaperone activity of αB-crystallin and prevented aggregation of the cataract-causing mutant αA3-G91del.The interactions between𝛼αB-crystallin and celastrol were detected by the FRET(Fluorescence Resonance Energy Transfer)technique,and verified by molecular docking.At least 9 binding patterns were recognized,and some binding sites covered the groove structure of αB-crystallin.Interestingly,αB-R120G,a cataract-causing mutation located at the groove structure,and celastrol can decrease the aggregates of αB-R120G.Overall,our results suggested celastrol not only promoted the formation of large αB-crystallin oligomers,which enhanced its chaperone activity,but also bound to the groove structure of its α-crystallin domain to maintain its structural stability.Celastrol might serve as a chemical and pharmacological chaperone for cataract treatment.展开更多
Background:Refractive errors,particularly myopia,are the leading visual disorders worldwide,significantly affecting the quality of life(QOL)even after correction.This scoping review focuses on health-related quality o...Background:Refractive errors,particularly myopia,are the leading visual disorders worldwide,significantly affecting the quality of life(QOL)even after correction.This scoping review focuses on health-related quality of life(HRQOL)measurements for children and adolescents with refractive errors.Main text:We explored generic and disease-specific HRQOL tools,examining their content,psychometric prop-erties,and the impact of various interventions on QOL.Two English databases—PubMed,Embase,and one Chinese database,CNKI,were searched for relevant studies published from January 2001 to October 2023.In-clusion criteria encompassed studies using standardized instruments to assess the QOL of children aged 0–18 with refractive errors.The review compares prevalent HRQOL measurements,analyzes children's refractive error as-sessments,and discusses intervention effects on patient QOL.Conclusions:The study underlines the necessity of developing disease-specific QOL instruments for very young children and serves as a practical guide for researchers in this field.The findings advocate for a targeted approach in HRQOL assessment among the pediatric population,identifying critical gaps in current methodologies.展开更多
The binding energy spectra and electron momentum distributions(EMDs)of valence orbitals in toluene molecule were measured by(e,2e)electron momentum spectrometer.A comprehensive analysis of molecular vibrational effect...The binding energy spectra and electron momentum distributions(EMDs)of valence orbitals in toluene molecule were measured by(e,2e)electron momentum spectrometer.A comprehensive analysis of molecular vibrational effects on the EMDs was conducted through harmonic analytical quantum mechanical approach calculations and molecular dynamics simulations within the plane wave impulse approximation(PWIA).Furthermore,the multicenter three-distorted-wave method was employed to investigate the validity of the PWIA.A comparison between experimental measurements and theoretical predictions demonstrates that molecular vibrations have negligible effects on the EMDs,whereas the distorted-wave effects are obvious,particularly in large momentum regions.展开更多
Wearable biopotential sensing devices are essential to long-term and real-time monitoring of human health.Non-contact,capacitive sensing electrodes prevent potential skin iritations,and are thus beneficial for long-te...Wearable biopotential sensing devices are essential to long-term and real-time monitoring of human health.Non-contact,capacitive sensing electrodes prevent potential skin iritations,and are thus beneficial for long-term monitoring.Existing capacitive electrodes are either connected to a separate control circuit via external wires or have limited sensing capacitances,which leads to low signal qualities.This study demonstrates a stretchable capacitive sensing device with integrated electrodes and control electronics,with enhanced signal qualities.The electrodes and the control electronics are fabricated on a common fabric substrate for breathability and strain-limiting protection.The stretchable electrodes are based on an island-bridge design with a stretchability as high as-100%,and an area ratio as high as-80%.By using a dielectric calcium copper titanate(CCTO)composite as the adhesive layer,the electrode capacitance can be increased,yielding an enhanced signal-to-noise ratio(SNR)in the acquired biopotentials.This device offers a convenient and comfortable approach for long-term non-contact monitoring of biopotential signals.展开更多
The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the...The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell mass.Between June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were recruited.ELVs were isolated by ultra-centrifugation.The proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and ELISA.The role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 preadipocytes.Circulating ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and inflammation.The protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic conditions.The ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling pathway.Moreover,Omentin-1 in ELVs was downregulated by insulin.The circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell proliferation.This effect was associated with downregulated Omentin-1 protein in ELVs.This newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM.展开更多
Corneal collagen cross-linking(CXL)is a therapeutic procedure aiming at increasing the corneal stiffness in the keratoconus eyes by induction of cross-links within the extracellular matrix.It is achieved by ultraviole...Corneal collagen cross-linking(CXL)is a therapeutic procedure aiming at increasing the corneal stiffness in the keratoconus eyes by induction of cross-links within the extracellular matrix.It is achieved by ultraviolet-A(370 nm)irradiation of the cornea after saturation with the photosensitizer riboflavin.In the conventional CXL protocol,a minimum de-epithelialized corneal thickness of 400μm is recommended to avoid potential irradiation damage to the corneal endothelium.In advanced keratoconus,however,stromal thickness is often lower than 400μm,which limits the application of CXL in that category.Efforts have been undertaken to modify the conventional CXL procedure to be applicable in thin corneas.The current review discusses different techniques employed to achieve this end and their results.The overall safety and efficacy of the modified CXL protocols are good,as most of them managed to halt the progression of keratectasia without postoperative complications.However,the evidence of safety and efficacy in the use of modified CXL protocols is still limited to few studies with few patients involved.Controlled studies with long-term follow-up are required to confirm the safety and efficacy of the modified protocols.展开更多
Slurry flow and proppant placement in irregular fractures are crucial to evaluate hydraulic fracturing stimulation but need to be better understood.This study aims to investigate how irregular fracture affects proppan...Slurry flow and proppant placement in irregular fractures are crucial to evaluate hydraulic fracturing stimulation but need to be better understood.This study aims to investigate how irregular fracture affects proppant transport and distribution using laboratory experiments and micro-scale numerical models.The unresolved method of the computational fluid dynamics(CFD)and the discrete element method(DEM)considers Saffman lift force,Magnus force,and virtual mass force to accurately capture the frequent interaction between proppant and slickwater.Experimental results validated the reliability of the optimized CFD-DEM model and calibrated primary parameters.The effects of crack height and width,bending angle,and distance between the crack and inlet on particle distribution were studied.The results indicated that the improved numerical method could rationally simulate proppant transport in fractures at a scale factor.The small crack height causes downward and upward flows,which wash proppant to the fracture rear and form isolated proppant dunes.A wider region in the fracture is beneficial to build up a large dune,and the high dune can hinder particle transport into the fracture rear.When the crack is close to the inlet,the primary fracture without proppants will close to hinder gas production.The smaller the bending angle,the smaller the proppant dune.A regression model can precisely predict the dune coverage ratio.The results fundamentally understand how complex fractures and natural cracks affect slurry flow and proppant distribution.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant No.12127804).
文摘We present a comprehensive electron momentum spectroscopy study on the electronic structure of trifluorobromomethane.The binding energy spectrum and electron momentum profiles of the entire outer-valence orbitals and the first inner-valence orbital along with several shake-up states were measured by using a high-sensitivity(e,2e)apparatus at an electron impact energy of 1213 eV.Theoretical calculations employing the density functional theory with B3LYP hybrid functional and the symmetry-adapted cluster configuration-interaction method were performed to interpret the experimental results.Important effects of electron correlations in the initial neutral and final ionic states on the electron momentum profiles have been observed.
基金supported by the National Key Research and Development Program of China(No.2022YFA1602502)the National Natural Science Foundation of China(No.12127804).
文摘The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^(2+),O_(2)^(2+)O^(+),O^(2+),and their total)and two Coulomb explosion channels(O^(+)+O^(+)and O^(2+)+O^(+))are obtained by putting the data of O^(2+)on the scale of Ar+from O_(2)and Ar gases mixed with a fixed relative flow ratio of 1:1.The experimental errors are assessed by taking uncertainties of various factors into account.The present absolute cross sections are well consistent with the previous data in the overlapped energy range below 1000 eV.
基金supported by the National Key Research and Development Program of China (Grant No.2022YFA 1602500)the National Natural Science Foundation of China (Grant Nos.11934004 and 12241410).
文摘The semiclassical non-perturbative atomic orbital close-coupling approach has been employed to study the electron capture and excitation processes in He^(2+)-H(1s)and He^(2+)-H(2s)collision systems.In order to ensure the accuracy of our calculated cross sections,a large number of high excited states and pseudostates are included in the expansion basis sets which are centered on the target and projectile,respectively.The total and partial charge transfer and excitation cross sections are obtained for a wide-energy domain ranging from 1 keV/amu to 200 keV/amu.The present calculations are also compared with the results from other theoretical methods.These cross section data are useful for the investigation of astrophysics and laboratory plasma.
基金supported by the National Natural Science Foundation of China(Grant Nos.:32271311 and 82200784 to Lidan Hu).
文摘Diabetes mellitus(DM)is a major metabolic disease endangering global health,with diabetic nephropathy(DN)as a primary complication lacking curative therapy.Sporoderm-broken spores of Ganoderma lucidum(GLP),an herbal medicine,has been used for the treatment of metabolic disorders.In this study,DN was induced in Sprague-Dawley rats using streptozotocin(STZ)and a high-fat diet(HFD),and the protective mechanisms of GLP were investigated through transcriptomic,metabolomic,and network pharmacology(NP)analyses.Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats.Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes.GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression(Igfbp1 and Angptl4)and enhanced metabolic biomarkers of 4-Aminocatechol.In addition,NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways.In conclusion,this study provides supportive evidence of the anti-inflammatory effects of GLP supplements,highlighting their potential for promising clinical applications in treating DN.
基金supported by QingLan Project of Jiangsu Province and National Science Fund of China(Nos.61806088,61902160)was supported by Changzhou Science and Technology Support Plan(No.CE20185044).
文摘Studies show that encoding technologies in H.264/AVC,including prediction and conversion,are essential technologies.However,these technologies are more complicated than the MPEG-4,which is a standard method and widely adopted worldwide.Therefore,the amount of calculation in H.264/AVC is significantly up-regulated compared to that of the MPEG-4.In the present study,it is intended to simplify the computational expenses in the international standard compression coding system H.264/AVC for moving images.Inter prediction refers to the most feasible compression technology,taking up to 60%of the entire encoding.In this regard,prediction error and motion vector information are proposed to simplify the computation of inter predictive coding technology.In the initial frame,motion compensation is performed in all target modes and then basic information is collected and analyzed.After the initial frame,motion compensation is performed only in the middle 8×8 modes,and the basic information amount shifts.In order to evaluate the effectiveness of the proposed method and assess the motion image compression coding,four types of motion images,defined by the international telecommunication union(ITU),are employed.Based on the obtained results,it is concluded that the developed method is capable of simplifying the calculation,while it is slightly affected by the inferior image quality and the amount of information.
文摘Objective： The aim of this study was to evaluate the value of the application of anterolateral thigh flap in postoperative repairing of vulva tumor. Methods： Anterolateral thigh flap with the descending branch of the lateral circumflex femoral artery and the pedicle of anterolaterat thigh cutaneous nerve was used in primary repairing of the defect from postoperative vulva tumor, the sizes of the flaps ranged from 10 cm ×14 cm-14 cm× 20 cm. Results： All flaps survived, during a follow-up of 2 to 12 months, the appearance of the flaps was satisfactory with sensitive function without local recurrences. Conclusion： Anterelateral thigh flap has fairly long vessel pedicle, wider vessel diameter, and is in the covert area. Anterolateral thigh flap with sensory nerve is the ideal choice for postoperative repairing of vulva tumor.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFA0402300).
文摘The outer valence electron momentum distributions of benzene are reinvestigated with theoretical calculations involving the vibrational effects.The results are compared with recent experimental measurements[Phys.Rev.A 98042705(2018)].The significant discrepancies between theories and experiments in previous works have now been interpreted quantitatively,indicating that the vibrational motion in benzene molecule has noticeable influence on its electron momentum distributions.
基金Project supported by the National Key Research and Development Program of China(Grant Nos.2017YFA0402300 and 2017YFA0303501)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB34000000)。
文摘We report the study on the complete three-body Coulomb explosion(CE)of N_(2)O^(q+)(q=5,6)induced by 56-keV/u Ne8+ion collision with N2O gaseous molecule.Six CE channels for N_(2)O^(5+)and seven for N_(2)O^(6+)are identified by measuring three ionic fragments and the charge-changed projectile in quadruple coincidence.Correspondingly the kinetic energy release(KER)and momentum correlation angle(MCA)distributions of three ionic fragments for each of the CE channels are also deduced.Numerical computation is presented to reconstruct the geometric structure of N_(2)O^(q+0prior to dissociation based on the measured KER and MCA.The N–N and N–O bond lengths and the N–N–O bond angles of N_(2)O^(q+)for each of the channels are determined.
基金the National Natural Science Foundation of China(Grant Nos.12004370,11534011,and 11934004)the National Key Research and Development Program of China(Grant Nos.2017YFA0402300 and 2019YFA0210004).
文摘We report theoretical studies of electron impact triple differential cross sections of two bio-molecules,pyrimidine and tetrahydrofurfuryl alcohol,in the coplanar asymmetric kinematic conditions with the impact energy of 250 eV and ejected electron energy of 20 eV at three scattering angles of-5°,-10°,and-15°.Present multi-center distorted-wave method well describes the experimental data,which was obtained by performing(e,2e)experiment.The calculations show that the secondary electron produced by the primary impact electron is strongly influenced by the molecular ionic multi-center potential,which must be considered when the low energy electron interacts with DNA analogues.
基金supported by the National Key Research and Development Program of China(No.2017YFA0303500)the National Natural Science Foundation of China(No.11674302)。
文摘In this work,electron energy spectroscopic mapping of surface plasmon of Ag nanostructures on highly oriented pyrolytic graphite is reported.Benefitting from the angular dispersive feature of the present scanning probe electron energy spectrometer,a multi-channel detection mode is developed.By scanning along one direction,the two-dimensional intensity distribution of Ag surface plasmon excitation due to the collision of electron emitted from the tip can be obtained in parallel.The spectroscopic spatial resolution is determined to be around 80 nm.
基金funded by the Project from the Science Technology Department of Zhejiang Province[No.LGC19H120002]the Natural Science Foundation of Zhejiang Province[No.LQ22C070004]National Natural Science Foundation of China[No.82271065 and No.82471154].
文摘Background Retinitis pigmentosa(RP)is a genetically and clinically heterogeneous group of hereditary degenerative disorders affecting approximately one in every 4000 people worldwide.Abnormalities in the retina's photoreceptors can cause night blindness or even complete vision loss.Retinitis Pigmentosa 1(RP1),also known as the oxygen-regulated protein-1,is a microtubule-associated protein(MAP)that organizes the outer segment of the photoreceptor.Besides,mutations in the RP1 gene are associated with dominant or recessive form of RP.This study aims to identify the potential pathogenic genes in Chinese RP patients and to elucidate the association relationship between the mutant gene and the phenotypes.Methods Multiple ophthalmic examinations,whole-exome sequencing,sanger sequencing,and in silico analysis were performed to evaluate the clinical features and pathogenic genes in a five-generation Chinese family diagnosed with RP.Results Our findings revealed a novel truncating mutation c.2015_2018del p.(Lys672Argfs∗9)in RP1 that may result in the translation of a protein with deleterious effects on photoreceptors.Therefore,resulting in autosomal dominant retinitis pigmentosa(ADRP).Conclusions This study broaden the range of genetic mutations associated with RP1 in ADRP and make a valuable contribution to the ongoing endeavors aimed at characterizing the molecular aspects of Chinese ADRP.Future studies would pay more attention in determining the characterization of the mutantations in RP1 gene and the relationship between genotype and phenotype in RP patients.
基金supported by the National Natural Science Foundation of China(31872724,and 81900837)the Natural Science Foundation of Zhejiang Province(LR21H120001).
文摘Protein misfolding and aggregation are crucial pathogenic factors for cataracts,which are the leading cause of visual impairment worldwide.α-crystallin,as a small molecular chaperone,is involved in preventing protein misfolding and maintaining lens transparency.The chaperone activity of α-crystallin depends on its oligomeric state.Our previous work identified a natural compound,celastrol,which could regulate the oligomeric state of αB-crystallin.In this work,based on the UNcle and SEC analysis,we found that celastrol induced𝛼αB-crystallin to form large oligomers.Large oligomer formation enhanced the chaperone activity of αB-crystallin and prevented aggregation of the cataract-causing mutant αA3-G91del.The interactions between𝛼αB-crystallin and celastrol were detected by the FRET(Fluorescence Resonance Energy Transfer)technique,and verified by molecular docking.At least 9 binding patterns were recognized,and some binding sites covered the groove structure of αB-crystallin.Interestingly,αB-R120G,a cataract-causing mutation located at the groove structure,and celastrol can decrease the aggregates of αB-R120G.Overall,our results suggested celastrol not only promoted the formation of large αB-crystallin oligomers,which enhanced its chaperone activity,but also bound to the groove structure of its α-crystallin domain to maintain its structural stability.Celastrol might serve as a chemical and pharmacological chaperone for cataract treatment.
基金the Natural Science Foundation of Zhejiang Province under grant number LQ22C070004.
文摘Background:Refractive errors,particularly myopia,are the leading visual disorders worldwide,significantly affecting the quality of life(QOL)even after correction.This scoping review focuses on health-related quality of life(HRQOL)measurements for children and adolescents with refractive errors.Main text:We explored generic and disease-specific HRQOL tools,examining their content,psychometric prop-erties,and the impact of various interventions on QOL.Two English databases—PubMed,Embase,and one Chinese database,CNKI,were searched for relevant studies published from January 2001 to October 2023.In-clusion criteria encompassed studies using standardized instruments to assess the QOL of children aged 0–18 with refractive errors.The review compares prevalent HRQOL measurements,analyzes children's refractive error as-sessments,and discusses intervention effects on patient QOL.Conclusions:The study underlines the necessity of developing disease-specific QOL instruments for very young children and serves as a practical guide for researchers in this field.The findings advocate for a targeted approach in HRQOL assessment among the pediatric population,identifying critical gaps in current methodologies.
基金supported by the National Natural Science Foundation of China(Grant No.12127804)the Supercomputing Center of University of Science and Technology of China.
文摘The binding energy spectra and electron momentum distributions(EMDs)of valence orbitals in toluene molecule were measured by(e,2e)electron momentum spectrometer.A comprehensive analysis of molecular vibrational effects on the EMDs was conducted through harmonic analytical quantum mechanical approach calculations and molecular dynamics simulations within the plane wave impulse approximation(PWIA).Furthermore,the multicenter three-distorted-wave method was employed to investigate the validity of the PWIA.A comparison between experimental measurements and theoretical predictions demonstrates that molecular vibrations have negligible effects on the EMDs,whereas the distorted-wave effects are obvious,particularly in large momentum regions.
基金research sponsored by Air Force Research Laboratory under agreement number FA8650-18-2-5402.
文摘Wearable biopotential sensing devices are essential to long-term and real-time monitoring of human health.Non-contact,capacitive sensing electrodes prevent potential skin iritations,and are thus beneficial for long-term monitoring.Existing capacitive electrodes are either connected to a separate control circuit via external wires or have limited sensing capacitances,which leads to low signal qualities.This study demonstrates a stretchable capacitive sensing device with integrated electrodes and control electronics,with enhanced signal qualities.The electrodes and the control electronics are fabricated on a common fabric substrate for breathability and strain-limiting protection.The stretchable electrodes are based on an island-bridge design with a stretchability as high as-100%,and an area ratio as high as-80%.By using a dielectric calcium copper titanate(CCTO)composite as the adhesive layer,the electrode capacitance can be increased,yielding an enhanced signal-to-noise ratio(SNR)in the acquired biopotentials.This device offers a convenient and comfortable approach for long-term non-contact monitoring of biopotential signals.
基金This work was supported by the National Key R&D Program of China(No.2018YFA0800401)the National Natural Science Foundation of China(No.81200629,81570763,81770861 and 31571401)+2 种基金the Fundamental Science and Advanced Technology Research of Chongqing(Major Project,No.CSTC2015jcyjB0146)the Chongqing Science and Technology Foundation(No.cstc2018jcyjAX0232)the Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJZD-K201800402).
文摘The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell mass.Between June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were recruited.ELVs were isolated by ultra-centrifugation.The proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and ELISA.The role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 preadipocytes.Circulating ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and inflammation.The protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic conditions.The ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling pathway.Moreover,Omentin-1 in ELVs was downregulated by insulin.The circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell proliferation.This effect was associated with downregulated Omentin-1 protein in ELVs.This newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM.
文摘Corneal collagen cross-linking(CXL)is a therapeutic procedure aiming at increasing the corneal stiffness in the keratoconus eyes by induction of cross-links within the extracellular matrix.It is achieved by ultraviolet-A(370 nm)irradiation of the cornea after saturation with the photosensitizer riboflavin.In the conventional CXL protocol,a minimum de-epithelialized corneal thickness of 400μm is recommended to avoid potential irradiation damage to the corneal endothelium.In advanced keratoconus,however,stromal thickness is often lower than 400μm,which limits the application of CXL in that category.Efforts have been undertaken to modify the conventional CXL procedure to be applicable in thin corneas.The current review discusses different techniques employed to achieve this end and their results.The overall safety and efficacy of the modified CXL protocols are good,as most of them managed to halt the progression of keratectasia without postoperative complications.However,the evidence of safety and efficacy in the use of modified CXL protocols is still limited to few studies with few patients involved.Controlled studies with long-term follow-up are required to confirm the safety and efficacy of the modified protocols.
基金This work was supported by the National Natural Science Foundation of China(grant No.52274035).
文摘Slurry flow and proppant placement in irregular fractures are crucial to evaluate hydraulic fracturing stimulation but need to be better understood.This study aims to investigate how irregular fracture affects proppant transport and distribution using laboratory experiments and micro-scale numerical models.The unresolved method of the computational fluid dynamics(CFD)and the discrete element method(DEM)considers Saffman lift force,Magnus force,and virtual mass force to accurately capture the frequent interaction between proppant and slickwater.Experimental results validated the reliability of the optimized CFD-DEM model and calibrated primary parameters.The effects of crack height and width,bending angle,and distance between the crack and inlet on particle distribution were studied.The results indicated that the improved numerical method could rationally simulate proppant transport in fractures at a scale factor.The small crack height causes downward and upward flows,which wash proppant to the fracture rear and form isolated proppant dunes.A wider region in the fracture is beneficial to build up a large dune,and the high dune can hinder particle transport into the fracture rear.When the crack is close to the inlet,the primary fracture without proppants will close to hinder gas production.The smaller the bending angle,the smaller the proppant dune.A regression model can precisely predict the dune coverage ratio.The results fundamentally understand how complex fractures and natural cracks affect slurry flow and proppant distribution.
