Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and ...Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.展开更多
Sirtuins(SIRTs) are nicotinamide adenine dinucleotide(NAD^+)-dependent protein deacetylases,which regulate important biological processes ranging from apoptosis,age-associated pathophysiologies,adipocyte and muscle di...Sirtuins(SIRTs) are nicotinamide adenine dinucleotide(NAD^+)-dependent protein deacetylases,which regulate important biological processes ranging from apoptosis,age-associated pathophysiologies,adipocyte and muscle differentiation,and energy expenditure to gluconeogenesis.Very recently,sirtuin 5(SIRT5) has received considerable attention due to that it was found to have weak deacetylase activity but strong desuccinylase,demalonylase and deglutarylase activities,and it was also found to be associated with several human diseases such as cancer,Alzheimer's disease,and Parkinson's disease.In this review,we for the first time summarized the structure characteristics,known peptide and small-molecule inhibitors of SIRT5,extracted some clues from current available information and introduced some feasible,practical in silico methods,which might be useful in further efforts to develop new SIRT5 inhibitors.展开更多
Chronic inflammatory bowel disease(IBD)is a debilitating inflammatory disorder that significantly impacts patients’quality of life.Exploring natural dietary interventions to regulate gut microbiota and mitigate IBD i...Chronic inflammatory bowel disease(IBD)is a debilitating inflammatory disorder that significantly impacts patients’quality of life.Exploring natural dietary interventions to regulate gut microbiota and mitigate IBD is great significance.This study investigates the role of a sea buckthorn pulp oil(SBPO)nanoemulsion,formulated with a NaCas-Salecanβ-glucan conjugate,in alleviating dextran sulfate sodium(DSS)-induced colitis in C57BL/6 male mice.The nanoemulsion via gavage for 37 days(15μL/10 g BW)significantly reduced colitis symptoms,restored colon length,and lowered the disease activity index(P<0.05).It also significantly inhibited the level of pro-inflammatory cytokines,enhanced antioxidant enzyme activities,and increased short-chain fatty acids(SCFAs)level(P<0.05).High-throughput sequencing revealed that the nanoemulsion can restored gut microbiota balance.Non-targeted fecal metabolomics showed that the nanoemulsion modulated amino acid,organic acid,and purine metabolic pathways.Compared to SBPO alone,the nanoemulsion showed superior effects in alleviating colitis.These findings support the development of SBPO-based functional foods and provide new insights into potential clinical treatments for IBD.展开更多
基金supported by the National Natural Science Foundation of China (31801647)Sichuan Science and Technology Program (2018JY0194,2020YFN0153,2020YFN0151)。
文摘Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.
基金supported by the Chun hui of Ministry of Education Project(Z2015120)the National Natural Science Foundation of China(81502989)the China Postdoctoral Science Foundation Funded Project(2015M570789)
文摘Sirtuins(SIRTs) are nicotinamide adenine dinucleotide(NAD^+)-dependent protein deacetylases,which regulate important biological processes ranging from apoptosis,age-associated pathophysiologies,adipocyte and muscle differentiation,and energy expenditure to gluconeogenesis.Very recently,sirtuin 5(SIRT5) has received considerable attention due to that it was found to have weak deacetylase activity but strong desuccinylase,demalonylase and deglutarylase activities,and it was also found to be associated with several human diseases such as cancer,Alzheimer's disease,and Parkinson's disease.In this review,we for the first time summarized the structure characteristics,known peptide and small-molecule inhibitors of SIRT5,extracted some clues from current available information and introduced some feasible,practical in silico methods,which might be useful in further efforts to develop new SIRT5 inhibitors.
基金supported by the National Natural Science Foundation of China(3197160117)the Science and Technology Program of Sichuan Province(2022JDTD0028,2022YFN0016).
文摘Chronic inflammatory bowel disease(IBD)is a debilitating inflammatory disorder that significantly impacts patients’quality of life.Exploring natural dietary interventions to regulate gut microbiota and mitigate IBD is great significance.This study investigates the role of a sea buckthorn pulp oil(SBPO)nanoemulsion,formulated with a NaCas-Salecanβ-glucan conjugate,in alleviating dextran sulfate sodium(DSS)-induced colitis in C57BL/6 male mice.The nanoemulsion via gavage for 37 days(15μL/10 g BW)significantly reduced colitis symptoms,restored colon length,and lowered the disease activity index(P<0.05).It also significantly inhibited the level of pro-inflammatory cytokines,enhanced antioxidant enzyme activities,and increased short-chain fatty acids(SCFAs)level(P<0.05).High-throughput sequencing revealed that the nanoemulsion can restored gut microbiota balance.Non-targeted fecal metabolomics showed that the nanoemulsion modulated amino acid,organic acid,and purine metabolic pathways.Compared to SBPO alone,the nanoemulsion showed superior effects in alleviating colitis.These findings support the development of SBPO-based functional foods and provide new insights into potential clinical treatments for IBD.
