Medicinal foods(MFs)have been extensively documented in ancient literature worldwide.As the terminology has become increasingly relevant in a population shaped by nutritional excess and sub-healthy status,modern studi...Medicinal foods(MFs)have been extensively documented in ancient literature worldwide.As the terminology has become increasingly relevant in a population shaped by nutritional excess and sub-healthy status,modern studies on these items are imperative.Current omics tools generate sufficiently high-throughput data for molecular-level interaction mechanisms,which contribute to MFs’utilization.This study focused on the applications and trends of multi-omics studies on MFs.Four aspects of the topic were reviewed:resource mining,processing,quality control,and health regulation.Metabolomics was the most applied omics tool in MFs research,especially in the discovery of critical components and adulterants.Furthermore,this work recognized the significance of biosynthetic pathway discovery,given the rapid advances in bioinformatics and biotechnology tools.Since high-throughput data can be obtained through omics studies,it is imperative to guide future multi-omics research to develop a consolidated view of MFs and their prospects.展开更多
Cinnamaldehyde(CA)is the key component of the essential oil derived from cinnamon bark and recommended as a health factor in foods for the prevention and intervention of metabolic disorders.This research aims to revea...Cinnamaldehyde(CA)is the key component of the essential oil derived from cinnamon bark and recommended as a health factor in foods for the prevention and intervention of metabolic disorders.This research aims to reveal the association between the difference in the electrophilic abilities of functional groups in CA and 3-phenylpro-pionaldehyde(PA),and their regulation of lipid peroxidation as well as energy metabolism in free fatty acids(FFAs)overloaded HepG2 cells.CA treatment reduced lipid accumulation and synthesis-related factors,increased levels of factors associated with fatty acid β-oxidation,decreased reactive oxygen species(ROS)content,and restored mitochondrial function and was superior to PA.Moreover,CA treatment increased nuclear Nrf2 protein levels and regulated the nuclear/cytoplasmic Nrf2 ratio,which suggested that CA effectively upregulated the Nrf2 signaling pathway.Furthermore,molecular docking showed that CA possessed an electrophilic index of 2.45 covalently bound to Nrf2-associated Keap1 protein with an affinity of3.6 kcal/mol,which was much stronger than PA,and HPLC-MS/MS analysis further confirmed that CA covalently modified Cys151 on Keap1 protein.In conclusion,CA has the capacity to attenuate hepatic steatosis and cellular oxidative response via Nrf2 signaling pathway,which is closely related to its strong electrophilic ability.展开更多
This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin(WG).The allergenicity-guided assay revealed walnut globulin(WG)from walnut defatted powder ...This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin(WG).The allergenicity-guided assay revealed walnut globulin(WG)from walnut defatted powder without pellicle was the most allergenic protein fraction of walnut.Complexes of phenolic extracts from walnut pellicle(PEWP)and WG were prepared in different ratio and pH conditions to further investigate the effect of PEWP on the protein structure and allergenicity of globulin.The covalent interaction between PEWP and WG in pH 7 was lower than pH 11,according to the free amino groups’content and SDS-PAGE analysis.Comprehensive methods characterized the complexes,including UV-Visible,fluorescence,Fourier transform infrared,and circular dichroism spectroscopy,and indicated that combination of polyphenols with protein altered the secondary and tertiary structure of the protein,leading to the unfolding of the protein structure.Meanwhile,the combination with PEWP reduced the IgG-binding capacity of WG in a dose-dependent manner,reaching a minimum of 26.90±4.31%(at pH 7),which was significantly(P<0.05)lower compared to that at pH 11(41.79±1.25%).Correlation analysis revealed a significant correlation between IgG-binding capacity and protein structure,as well as the addition concentration of PEWP(r=-0.85,P<0.01),and pH conditions may indirectly influence the structure and allergenicity of the protein by affecting the interaction between WG and PEWP.The interaction between WG and PEWP was characterized through fluorescence-quenching mechanism analysis,indicating that polyphenols from PEWP mainly interact with WG through hydrogen bond and hydrophobic interaction.展开更多
Colla Corii Asini(Ejiao)peptide-iron(EPI)chelates formed during digestion have been revealed as potential hematopoietic components.However,it remains unclear how EPI regulates endogenous metabolism in vivo.Iron defici...Colla Corii Asini(Ejiao)peptide-iron(EPI)chelates formed during digestion have been revealed as potential hematopoietic components.However,it remains unclear how EPI regulates endogenous metabolism in vivo.Iron deficiency anemia(IDA)mice were gavaged with EPI chelates in a gradient dosage,while Ejiao hydrolysate-iron(EJI)chelates and FeSO4 were used as positive controls.The effect of EPI on hematopoietic recovery was evaluated.For the plasma untargeted metabolomic analysis,liquid chromatography-tandem mass spectrometry(LC-MS/MS)were applied to identify metabolites in the samples.EPI significantly increased the levels of hemogram in IDA mice in a dose-dependent manner.The relative iron bioavailability in EPI was 123.45%of that in FeSO4 in vivo.After high dose of EPI(EPI-H)supplementation,the levels of 22 potential metabolic markers in the IDA group returned to normal,including sphingomyelin and arachidonic acid.EPI-H affected endogenous metabolites by regulating the metabolism of energy,lipids,and amino acids,promoted hematopoiesis,regulated metabolic pathways,and improved iron homeostasis.It’s a comprehensive nutritional intervention of IDA by EPI supplementation.展开更多
基金supported by the Natural Science Foundation of China,China[Grant Number 31700301].
文摘Medicinal foods(MFs)have been extensively documented in ancient literature worldwide.As the terminology has become increasingly relevant in a population shaped by nutritional excess and sub-healthy status,modern studies on these items are imperative.Current omics tools generate sufficiently high-throughput data for molecular-level interaction mechanisms,which contribute to MFs’utilization.This study focused on the applications and trends of multi-omics studies on MFs.Four aspects of the topic were reviewed:resource mining,processing,quality control,and health regulation.Metabolomics was the most applied omics tool in MFs research,especially in the discovery of critical components and adulterants.Furthermore,this work recognized the significance of biosynthetic pathway discovery,given the rapid advances in bioinformatics and biotechnology tools.Since high-throughput data can be obtained through omics studies,it is imperative to guide future multi-omics research to develop a consolidated view of MFs and their prospects.
文摘Cinnamaldehyde(CA)is the key component of the essential oil derived from cinnamon bark and recommended as a health factor in foods for the prevention and intervention of metabolic disorders.This research aims to reveal the association between the difference in the electrophilic abilities of functional groups in CA and 3-phenylpro-pionaldehyde(PA),and their regulation of lipid peroxidation as well as energy metabolism in free fatty acids(FFAs)overloaded HepG2 cells.CA treatment reduced lipid accumulation and synthesis-related factors,increased levels of factors associated with fatty acid β-oxidation,decreased reactive oxygen species(ROS)content,and restored mitochondrial function and was superior to PA.Moreover,CA treatment increased nuclear Nrf2 protein levels and regulated the nuclear/cytoplasmic Nrf2 ratio,which suggested that CA effectively upregulated the Nrf2 signaling pathway.Furthermore,molecular docking showed that CA possessed an electrophilic index of 2.45 covalently bound to Nrf2-associated Keap1 protein with an affinity of3.6 kcal/mol,which was much stronger than PA,and HPLC-MS/MS analysis further confirmed that CA covalently modified Cys151 on Keap1 protein.In conclusion,CA has the capacity to attenuate hepatic steatosis and cellular oxidative response via Nrf2 signaling pathway,which is closely related to its strong electrophilic ability.
基金supported by the National Natural Science Foundation of China(31700301).
文摘This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin(WG).The allergenicity-guided assay revealed walnut globulin(WG)from walnut defatted powder without pellicle was the most allergenic protein fraction of walnut.Complexes of phenolic extracts from walnut pellicle(PEWP)and WG were prepared in different ratio and pH conditions to further investigate the effect of PEWP on the protein structure and allergenicity of globulin.The covalent interaction between PEWP and WG in pH 7 was lower than pH 11,according to the free amino groups’content and SDS-PAGE analysis.Comprehensive methods characterized the complexes,including UV-Visible,fluorescence,Fourier transform infrared,and circular dichroism spectroscopy,and indicated that combination of polyphenols with protein altered the secondary and tertiary structure of the protein,leading to the unfolding of the protein structure.Meanwhile,the combination with PEWP reduced the IgG-binding capacity of WG in a dose-dependent manner,reaching a minimum of 26.90±4.31%(at pH 7),which was significantly(P<0.05)lower compared to that at pH 11(41.79±1.25%).Correlation analysis revealed a significant correlation between IgG-binding capacity and protein structure,as well as the addition concentration of PEWP(r=-0.85,P<0.01),and pH conditions may indirectly influence the structure and allergenicity of the protein by affecting the interaction between WG and PEWP.The interaction between WG and PEWP was characterized through fluorescence-quenching mechanism analysis,indicating that polyphenols from PEWP mainly interact with WG through hydrogen bond and hydrophobic interaction.
基金supported by Open Project Program of State Key Laboratory of Food Science and Resources,Jiangnan University,China(No.SKLF-KF-202111)the National Natural Science Foundation of China(No.82103836).
文摘Colla Corii Asini(Ejiao)peptide-iron(EPI)chelates formed during digestion have been revealed as potential hematopoietic components.However,it remains unclear how EPI regulates endogenous metabolism in vivo.Iron deficiency anemia(IDA)mice were gavaged with EPI chelates in a gradient dosage,while Ejiao hydrolysate-iron(EJI)chelates and FeSO4 were used as positive controls.The effect of EPI on hematopoietic recovery was evaluated.For the plasma untargeted metabolomic analysis,liquid chromatography-tandem mass spectrometry(LC-MS/MS)were applied to identify metabolites in the samples.EPI significantly increased the levels of hemogram in IDA mice in a dose-dependent manner.The relative iron bioavailability in EPI was 123.45%of that in FeSO4 in vivo.After high dose of EPI(EPI-H)supplementation,the levels of 22 potential metabolic markers in the IDA group returned to normal,including sphingomyelin and arachidonic acid.EPI-H affected endogenous metabolites by regulating the metabolism of energy,lipids,and amino acids,promoted hematopoiesis,regulated metabolic pathways,and improved iron homeostasis.It’s a comprehensive nutritional intervention of IDA by EPI supplementation.
