目的探讨寿命蛋白(Mortalin)在子宫内膜癌(EC)中过表达的临床病理学意义。方法采用免疫组织化学EnVision法检测Mortalin蛋白在88例EC组织和37例正常子宫内膜组织中的表达,分析其过表达与EC临床病理特征之间的关系。应用UALCAN数据库分析...目的探讨寿命蛋白(Mortalin)在子宫内膜癌(EC)中过表达的临床病理学意义。方法采用免疫组织化学EnVision法检测Mortalin蛋白在88例EC组织和37例正常子宫内膜组织中的表达,分析其过表达与EC临床病理特征之间的关系。应用UALCAN数据库分析Mortalin mRNA在EC组织中的表达水平,应用The Human Protein Atlas和The Cancer Genome Atlas(TCGA)数据库进行生存分析。结果Mortalin蛋白主要表达于EC细胞的细胞质中;在EC组织中,Mortalin蛋白的阳性率和强阳性率分别为88.6%(78/88)和56.8%(50/88),高于正常子宫内膜组织(P<0.05)。Mortalin蛋白的表达与EC患者的病理分级、TNM分期和淋巴结转移相关(P<0.05),但与年龄无关(P>0.05)。UALCAN数据库分析显示,EC组织中的Mortalin mRNA表达高于正常子宫内膜组织(P<0.05)。The Human Protein Atlas和TCGA数据库生存分析结果显示,Mortalin蛋白和mRNA高表达的EC患者总生存期低于Mortalin蛋白和mRNA低表达患者(P<0.05)。结论Mortalin的表达与EC的发生、发展及预后相关,其有望成为EC患者靶向治疗及预后评估的重要指标。展开更多
Objective:Through epidemiological investigation,to investigate the etiology and influencing factors of primary gout and hyperuricemia(HUA).Methods:Using case-control methods,designed questionnaires on gout and/or HUA ...Objective:Through epidemiological investigation,to investigate the etiology and influencing factors of primary gout and hyperuricemia(HUA).Methods:Using case-control methods,designed questionnaires on gout and/or HUA related factors(living habits,eating habits and environmental factors,etc).Logistic regression analysis of the survey results was performed with SPSS 19.0 software.Results:(1)Univariate Logistic regression analysis:11 risk factors with statistical significance(P<0.05),often eat sauce food,often eat Fried food,often eat asparagus,often eat nuts,often drink,smoking,drink water<1000ml per day,residential temperature≤17℃in winter,work environment average temperature≤17℃in winter,often exposure to toxic and harmful substances,often use induction cooker or microwave oven(P<0.05),the OR values were 2.063,1.701,3.242,1.560,2.055,1.338,5.700,5.330,2.305,2.821,1.701;3 protective factors,occasionally eat fresh fruit,wake up between 5 and 7 am,use the computer less than 1 hour per day(P<0.05),OR values were 0.723,0.611 and 0.558,respectively.(2)Multivariate Logistic regression analysis was performed for all factors:6 risk factors,The risk factors were often drink,drink water<1000ml per day,residential temperature≤17℃in winter,work environment average temperature≤17℃in winter,use mobile network cellphones and use unicom network cellphones(P<0.05),OR values are 2.008,4.240,4.034,1.889,2.207 and 1.891,respectively;2 protective factors:occasionally eat agaric and use the computer less than 1 hour per day(P<0.05),OR values were 0.551 and 0.536,respectively.Conclusions:Proper improvement of diet structure,adjustment of life and behavior,and avoidance of environmental risk factors can effectively reduce uric acid level,control the incidence of HUA and gout,and improve health level.展开更多
Objective:To explore the tophi in different parts of the human body of microstructure and main chemical components.To lay the foundation for further revealing the inflammatory response mechanism of gout patients.Metho...Objective:To explore the tophi in different parts of the human body of microstructure and main chemical components.To lay the foundation for further revealing the inflammatory response mechanism of gout patients.Methods:Use microscope,scanning electron microscope,etc.to observe the microstructure of tophi,and use scanning electron microscope energy spectrum analysis,X-ray fluorescence analysis,X-ray diffraction analysis,etc.to detect and analyze the main crystalline components and chemical elements of tophi.Results:Through HE staining and scanning electron microscopy,the tophi is irregular block shape and its interior is needle shaped and radial.The energy spectrum analysis showed that the main elements of tophi were C,N,O,Na,etc.X-ray fluorescence analysis was used to detect tophi in the knees,elbows,and feet,the main elements of tophi were Cl,S,Ca,P,etc.X-ray diffraction analysis showed that the main crystalline components of tophi were sodium urate hydrate and sodium dihydrogen urate hydrate.Conclusion:The mainly elements of tophi are C,N,O,Na,Ca,Cl,S,P,etc.The main crystalline components of tophi were sodium urate hydrate and sodium dihydrogen urate hydrate.展开更多
Objective:To investigate the effect of pH value on uric acid (UA) solubility at room temperature (20-22℃), and to provide a theoretical basis for further understanding the dissolution and precipitation of UA in vivo....Objective:To investigate the effect of pH value on uric acid (UA) solubility at room temperature (20-22℃), and to provide a theoretical basis for further understanding the dissolution and precipitation of UA in vivo.Methods:UA was dissolved in NaOH solution and PBS solution respectively to prepare mother solutions, and then the mother solution was prepared into UA solutions with different concentration and pH value. The UA solutions at the same pH were taken the 1000 μmol/L as the UA standard solution. The absorbance value was measured by an ultraviolet spectrophotometer, and each sample was detected 3 times. The mean value was taken to establish the standard curve equation. Other sample solutions with different concentrations and pH values were tested at 0 h and 24 hrs, and repeated 3 times.Results:(1) In NaOH solution, the UA solubility of 1000, 2000, 3000 and 4000 μmol/L solution was detected at 0 h and 24 hrs, respectively. The results showed that the UA solubility increased with the pH value increase. However, in the sample solutions of 5000 and 6000μmol/L, the UA solubility increased with the pH increase at 0 h, and that increased and then decreased with the pH increase at 24 hrs. When the pH value was 7.0, the UA solubility was the highest, and that was basically consistent with the theoretical concentration. (2) In PBS solution, the UA solubility of 1000, 2000, 3000, 4000, 5000 and 6000 μmol/L was detected at 0 h. The results showed that the UA solubility increased with the pH value increase. When the pH value was 7.0 or above, the UA solubility that be measured was basically consistent with the theoretical concentration. While testing the above solution at 24 hrs, the results showed that each of the sample solution UA solubility in the pH 8.0, that measured concentrations are consistent with the basic theory of concentration. With the pH value decrease, the UA solubility decreased continuously. In the sample solutions of 1000, 2000 and 3000 μmol/L, when the pH value of 6.0 and 7.5, the UA solubility decreased significantly. In NaOH and PBS solutions, crystal precipitation occurred in all sample solutions except 1000 μmol/L. The crystals were determined as UA by infrared spectroscopy.Conclusion:The pH value can significantly affect the dissolution and precipitation of UA.展开更多
文摘目的探讨寿命蛋白(Mortalin)在子宫内膜癌(EC)中过表达的临床病理学意义。方法采用免疫组织化学EnVision法检测Mortalin蛋白在88例EC组织和37例正常子宫内膜组织中的表达,分析其过表达与EC临床病理特征之间的关系。应用UALCAN数据库分析Mortalin mRNA在EC组织中的表达水平,应用The Human Protein Atlas和The Cancer Genome Atlas(TCGA)数据库进行生存分析。结果Mortalin蛋白主要表达于EC细胞的细胞质中;在EC组织中,Mortalin蛋白的阳性率和强阳性率分别为88.6%(78/88)和56.8%(50/88),高于正常子宫内膜组织(P<0.05)。Mortalin蛋白的表达与EC患者的病理分级、TNM分期和淋巴结转移相关(P<0.05),但与年龄无关(P>0.05)。UALCAN数据库分析显示,EC组织中的Mortalin mRNA表达高于正常子宫内膜组织(P<0.05)。The Human Protein Atlas和TCGA数据库生存分析结果显示,Mortalin蛋白和mRNA高表达的EC患者总生存期低于Mortalin蛋白和mRNA低表达患者(P<0.05)。结论Mortalin的表达与EC的发生、发展及预后相关,其有望成为EC患者靶向治疗及预后评估的重要指标。
基金Heilongjiang natural science foundation project(No.H201374)
文摘Objective:Through epidemiological investigation,to investigate the etiology and influencing factors of primary gout and hyperuricemia(HUA).Methods:Using case-control methods,designed questionnaires on gout and/or HUA related factors(living habits,eating habits and environmental factors,etc).Logistic regression analysis of the survey results was performed with SPSS 19.0 software.Results:(1)Univariate Logistic regression analysis:11 risk factors with statistical significance(P<0.05),often eat sauce food,often eat Fried food,often eat asparagus,often eat nuts,often drink,smoking,drink water<1000ml per day,residential temperature≤17℃in winter,work environment average temperature≤17℃in winter,often exposure to toxic and harmful substances,often use induction cooker or microwave oven(P<0.05),the OR values were 2.063,1.701,3.242,1.560,2.055,1.338,5.700,5.330,2.305,2.821,1.701;3 protective factors,occasionally eat fresh fruit,wake up between 5 and 7 am,use the computer less than 1 hour per day(P<0.05),OR values were 0.723,0.611 and 0.558,respectively.(2)Multivariate Logistic regression analysis was performed for all factors:6 risk factors,The risk factors were often drink,drink water<1000ml per day,residential temperature≤17℃in winter,work environment average temperature≤17℃in winter,use mobile network cellphones and use unicom network cellphones(P<0.05),OR values are 2.008,4.240,4.034,1.889,2.207 and 1.891,respectively;2 protective factors:occasionally eat agaric and use the computer less than 1 hour per day(P<0.05),OR values were 0.551 and 0.536,respectively.Conclusions:Proper improvement of diet structure,adjustment of life and behavior,and avoidance of environmental risk factors can effectively reduce uric acid level,control the incidence of HUA and gout,and improve health level.
基金Jiamusi University Scientific Research Project(No.22zq201503)。
文摘Objective:To explore the tophi in different parts of the human body of microstructure and main chemical components.To lay the foundation for further revealing the inflammatory response mechanism of gout patients.Methods:Use microscope,scanning electron microscope,etc.to observe the microstructure of tophi,and use scanning electron microscope energy spectrum analysis,X-ray fluorescence analysis,X-ray diffraction analysis,etc.to detect and analyze the main crystalline components and chemical elements of tophi.Results:Through HE staining and scanning electron microscopy,the tophi is irregular block shape and its interior is needle shaped and radial.The energy spectrum analysis showed that the main elements of tophi were C,N,O,Na,etc.X-ray fluorescence analysis was used to detect tophi in the knees,elbows,and feet,the main elements of tophi were Cl,S,Ca,P,etc.X-ray diffraction analysis showed that the main crystalline components of tophi were sodium urate hydrate and sodium dihydrogen urate hydrate.Conclusion:The mainly elements of tophi are C,N,O,Na,Ca,Cl,S,P,etc.The main crystalline components of tophi were sodium urate hydrate and sodium dihydrogen urate hydrate.
基金Basic scientific research business fee project of university in Heilongjiang(2016-KYYWF-0591).
文摘Objective:To investigate the effect of pH value on uric acid (UA) solubility at room temperature (20-22℃), and to provide a theoretical basis for further understanding the dissolution and precipitation of UA in vivo.Methods:UA was dissolved in NaOH solution and PBS solution respectively to prepare mother solutions, and then the mother solution was prepared into UA solutions with different concentration and pH value. The UA solutions at the same pH were taken the 1000 μmol/L as the UA standard solution. The absorbance value was measured by an ultraviolet spectrophotometer, and each sample was detected 3 times. The mean value was taken to establish the standard curve equation. Other sample solutions with different concentrations and pH values were tested at 0 h and 24 hrs, and repeated 3 times.Results:(1) In NaOH solution, the UA solubility of 1000, 2000, 3000 and 4000 μmol/L solution was detected at 0 h and 24 hrs, respectively. The results showed that the UA solubility increased with the pH value increase. However, in the sample solutions of 5000 and 6000μmol/L, the UA solubility increased with the pH increase at 0 h, and that increased and then decreased with the pH increase at 24 hrs. When the pH value was 7.0, the UA solubility was the highest, and that was basically consistent with the theoretical concentration. (2) In PBS solution, the UA solubility of 1000, 2000, 3000, 4000, 5000 and 6000 μmol/L was detected at 0 h. The results showed that the UA solubility increased with the pH value increase. When the pH value was 7.0 or above, the UA solubility that be measured was basically consistent with the theoretical concentration. While testing the above solution at 24 hrs, the results showed that each of the sample solution UA solubility in the pH 8.0, that measured concentrations are consistent with the basic theory of concentration. With the pH value decrease, the UA solubility decreased continuously. In the sample solutions of 1000, 2000 and 3000 μmol/L, when the pH value of 6.0 and 7.5, the UA solubility decreased significantly. In NaOH and PBS solutions, crystal precipitation occurred in all sample solutions except 1000 μmol/L. The crystals were determined as UA by infrared spectroscopy.Conclusion:The pH value can significantly affect the dissolution and precipitation of UA.
