The biological pump,driven by phytoplankton production and death,plays a crucial role in the ocean’s sequestration of atmospheric CO_(2).In particular,marginal seas with high primary productivity show a significant c...The biological pump,driven by phytoplankton production and death,plays a crucial role in the ocean’s sequestration of atmospheric CO_(2).In particular,marginal seas with high primary productivity show a significant capacity for carbon fixation.Variations in phytoplankton biomass and community structure are key factors influencing the efficiency of the marine biological pump.The Taiwan Strait(TS)is a unique shallow conduit that connects the East China Sea(ECS)and the South China Sea(SCS),which are subject to seasonal monsoons and episodic events(e.g.,typhoons and floods).Thus,its planktonic ecosystem is significantly influenced by physical processes such as strong ocean currents,coastal upwelling and river discharge,resulting in noticeable seasonal variability.In this study,we examined spatiotemporal patterns of phytoplankton biomass and community structure using phytoplankton-sourced biomarkers from suspended particles in surface waters across all four seasons from 2019 to 2020 in the TS.The findings highlight notable seasonal disparities in phytoplankton biomass,with spring and summer exhibiting significantly higher levels compared to autumn and winter.In order to determine phytoplankton ecosystem responses to various physical and biological processes on a seasonal scale,we used Empirical Orthogonal/Eigen Function(EOF)analysis to investigate the covarying spatiotemporal patterns of:marine-sourced biomarkers and terrestrial-sourced biomarkers in surface suspended particles,a biomass indicator(Chl a),water-mass indicators[sea surface temperature(SST),sea surface salinity(SSS),nutrients],and a hydrodynamic indicator[total suspended solids at surface/bottom water,(TSS_S and TSS_B)].The results identified six physical-biological coupling modes that influence seasonal variations in marine phytoplankton ecosystems within the energetic strait system.Additionally,an in-depth understanding of the coupling between physical process and lipid biomarker signals from suspended particles in the contemporary marine environment can offer valuable insights for interpreting ancient sediment records of phytoplankton ecosystem evolution in the TS.展开更多
Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who atte...Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who attended Hospital 960 from January 2021 to January 2022 were retrospectively analyzed. Clinical data of the patients were collected, including gender, age, and histologic type. Immunohistochemical indexes such as Th17 cell level and monocyte/ lymphocyte ratio in the peripheral blood of patients were also collected. The prognosis of patients after treatment, as well as peripheral blood Th17 and MLR levels, were observed and analyzed. Results: After follow-up after treatment, in the final 74 patients, the prognosis was good in 32 patients, accounting for 43.24%, and the prognosis was bad in 42 patients, accounting for 56.76%. There were no significant differences between the average age and tumor diameters of the good prognosis and poor prognosis groups (P > 0.05). However, the TNM staging, intervention taken, differentiation degree, presence of distant metastasis, presence of lymph node metastasis, Th17 level, and MLR level are significantly different between the two groups (P < 0.05). Conclusion: Peripheral blood Th17 and MLR have predictive value for the prognosis of colorectal cancer patients, and high levels of peripheral blood Th17 and MLR imply poor prognosis. The detection of peripheral blood Th17 and MLR levels is simple and convenient and can be used as indicators to provide a reference for the prognostic assessment of colorectal cancer patients.展开更多
Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root o...Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.展开更多
To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene express...To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene expression data. Supervised learning approaches predicted that the Ras pathway is active in -70% of lung adenocarcinomas but inactive in most squamous cell carcinomas, pulmonary carcinoids, and small cell lung carcinomas. In contrast, the TGF-β, TNF-α, Src, Myc, E2F3, and β-catenin pathways are inactive in lung adenocarcinomas. We predicted an active Ras, Myc, Src, and/or E2F3 pathway in significant percentages of breast cancer, colorectal carcinoma, and gliomas. Our results also suggest that Ras may be the most prevailing oncogenic pathway. Additionally, many NCI60 cell lines exhibited a gene signature indicative of an active Ras, Myc, and/or Src, but not E2F3, β-catenin, TNF-α, or TGF-β pathway. To our knowledge, this is the first comprehensive survey of cancer pathway activities in nine major tumor types and the most widely used NCI60 cell lines. The ＂gene expression pathway signatures＂ we have defined could facilitate the understanding of molecular mechanisms in cancer development and provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screening.展开更多
Understanding the erosion–deposition process of sediments and the associated controlling mechanisms in subaqueous deltas is important for coastal environment protection.In this study,field observations and numerical ...Understanding the erosion–deposition process of sediments and the associated controlling mechanisms in subaqueous deltas is important for coastal environment protection.In this study,field observations and numerical simulations were performed for the Nanliu River subaqueous delta in Guangxi Province(Southern China)to investigate the sediment dynamic processes at the bottom boundary layer.The results show that the sediment resuspension mainly occurs during periods of spring tides and is mainly controlled by the wave action.When the seabed is free from erosion,suspended sediment settling caused by lateral transport is an important source of maintaining near bed suspended sediment concentration.It was also found that increasing the shear parameter could facilitate the formation of flocs,after which the small flocs tend to merge to large flocs.Finally,by performing a consistency analysis between the seabed erosion and deposition processes obtained from numerical simulation,and the changes of seabed level recorded by the equipment during the field observation,we determined that the local erosion coefficient was 5×10^(−5)kg/m^(2)·s.The one-dimensional simulation is also capable of revealing the general trend at the seabed where it is first subjected to erosion and then deposition,as indicated during the field measurements.展开更多
基金The National Key Research and Development Program of China under contract No.2019YFE0124700the Scientific Research Foundation of Third Institute of Oceanography,MNR under contract Nos 2019018 and 2019017+2 种基金the National Natural Science Foundation of China under contract Nos 42076038,U22A20585 and 41776099the Guangdong Basic and Applied Basic Research Foundation under contract No.2021A1515011886the STU Scientific Research Start-Up Foundation for Talents under contract No.NTF18011.
文摘The biological pump,driven by phytoplankton production and death,plays a crucial role in the ocean’s sequestration of atmospheric CO_(2).In particular,marginal seas with high primary productivity show a significant capacity for carbon fixation.Variations in phytoplankton biomass and community structure are key factors influencing the efficiency of the marine biological pump.The Taiwan Strait(TS)is a unique shallow conduit that connects the East China Sea(ECS)and the South China Sea(SCS),which are subject to seasonal monsoons and episodic events(e.g.,typhoons and floods).Thus,its planktonic ecosystem is significantly influenced by physical processes such as strong ocean currents,coastal upwelling and river discharge,resulting in noticeable seasonal variability.In this study,we examined spatiotemporal patterns of phytoplankton biomass and community structure using phytoplankton-sourced biomarkers from suspended particles in surface waters across all four seasons from 2019 to 2020 in the TS.The findings highlight notable seasonal disparities in phytoplankton biomass,with spring and summer exhibiting significantly higher levels compared to autumn and winter.In order to determine phytoplankton ecosystem responses to various physical and biological processes on a seasonal scale,we used Empirical Orthogonal/Eigen Function(EOF)analysis to investigate the covarying spatiotemporal patterns of:marine-sourced biomarkers and terrestrial-sourced biomarkers in surface suspended particles,a biomass indicator(Chl a),water-mass indicators[sea surface temperature(SST),sea surface salinity(SSS),nutrients],and a hydrodynamic indicator[total suspended solids at surface/bottom water,(TSS_S and TSS_B)].The results identified six physical-biological coupling modes that influence seasonal variations in marine phytoplankton ecosystems within the energetic strait system.Additionally,an in-depth understanding of the coupling between physical process and lipid biomarker signals from suspended particles in the contemporary marine environment can offer valuable insights for interpreting ancient sediment records of phytoplankton ecosystem evolution in the TS.
文摘Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who attended Hospital 960 from January 2021 to January 2022 were retrospectively analyzed. Clinical data of the patients were collected, including gender, age, and histologic type. Immunohistochemical indexes such as Th17 cell level and monocyte/ lymphocyte ratio in the peripheral blood of patients were also collected. The prognosis of patients after treatment, as well as peripheral blood Th17 and MLR levels, were observed and analyzed. Results: After follow-up after treatment, in the final 74 patients, the prognosis was good in 32 patients, accounting for 43.24%, and the prognosis was bad in 42 patients, accounting for 56.76%. There were no significant differences between the average age and tumor diameters of the good prognosis and poor prognosis groups (P > 0.05). However, the TNM staging, intervention taken, differentiation degree, presence of distant metastasis, presence of lymph node metastasis, Th17 level, and MLR level are significantly different between the two groups (P < 0.05). Conclusion: Peripheral blood Th17 and MLR have predictive value for the prognosis of colorectal cancer patients, and high levels of peripheral blood Th17 and MLR imply poor prognosis. The detection of peripheral blood Th17 and MLR levels is simple and convenient and can be used as indicators to provide a reference for the prognostic assessment of colorectal cancer patients.
基金This research was based on the‘Functional food research and development of Tetrastigma hemsleyanum’funded by the Shanghai Zhengyue Enterprise Management Co.,Ltd.and the Science and Technology Department of Zhejiang Province(No.2018C02045),China.
文摘Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.
文摘To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene expression data. Supervised learning approaches predicted that the Ras pathway is active in -70% of lung adenocarcinomas but inactive in most squamous cell carcinomas, pulmonary carcinoids, and small cell lung carcinomas. In contrast, the TGF-β, TNF-α, Src, Myc, E2F3, and β-catenin pathways are inactive in lung adenocarcinomas. We predicted an active Ras, Myc, Src, and/or E2F3 pathway in significant percentages of breast cancer, colorectal carcinoma, and gliomas. Our results also suggest that Ras may be the most prevailing oncogenic pathway. Additionally, many NCI60 cell lines exhibited a gene signature indicative of an active Ras, Myc, and/or Src, but not E2F3, β-catenin, TNF-α, or TGF-β pathway. To our knowledge, this is the first comprehensive survey of cancer pathway activities in nine major tumor types and the most widely used NCI60 cell lines. The ＂gene expression pathway signatures＂ we have defined could facilitate the understanding of molecular mechanisms in cancer development and provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screening.
基金supported by the Project Sponsored by the Scientific Research Foundation of Third Institute of Oceanography,SOA,China(Nos.2015007 and 2017015)the National Natural Science Foundation of China(Grant No.41776099).
文摘Understanding the erosion–deposition process of sediments and the associated controlling mechanisms in subaqueous deltas is important for coastal environment protection.In this study,field observations and numerical simulations were performed for the Nanliu River subaqueous delta in Guangxi Province(Southern China)to investigate the sediment dynamic processes at the bottom boundary layer.The results show that the sediment resuspension mainly occurs during periods of spring tides and is mainly controlled by the wave action.When the seabed is free from erosion,suspended sediment settling caused by lateral transport is an important source of maintaining near bed suspended sediment concentration.It was also found that increasing the shear parameter could facilitate the formation of flocs,after which the small flocs tend to merge to large flocs.Finally,by performing a consistency analysis between the seabed erosion and deposition processes obtained from numerical simulation,and the changes of seabed level recorded by the equipment during the field observation,we determined that the local erosion coefficient was 5×10^(−5)kg/m^(2)·s.The one-dimensional simulation is also capable of revealing the general trend at the seabed where it is first subjected to erosion and then deposition,as indicated during the field measurements.
