V-raf-leukemia viral oncogene 1(RAF1),a serine/threonine protein kinase,is well established to play a crucial role in tumorigenesis and cell development.However,the specific role of hypothalamic RAF1 in regulating ene...V-raf-leukemia viral oncogene 1(RAF1),a serine/threonine protein kinase,is well established to play a crucial role in tumorigenesis and cell development.However,the specific role of hypothalamic RAF1 in regulating energy metabolism remains unknown.In this study,we found that the expression of RAF1 was significantly increased in hypothalamic AgRP neurons of diet-induced obesity(DIO)mice.Under normal chow diet feeding,overexpression of Raf1 in AgRP neurons led to obesity in mice characterized by increased body weight,fat mass,and impaired glucose tolerance.Conversely,Raf1 knockout in AgRP neurons protected against diet-induced obesity,reducing fat mass and improving glucose tolerance.Mechanistically,Raf1 activated the MAPK signaling pathway,culminating in the phosphorylation of cAMP response element-binding protein(CREB),which enhanced transcription of Agrp and Npy.Insulin stimulation further potentiated the RAF1-MEK1/2-ERK1/2-CREB axis,highlighting RAF1's role in integrating hormonal and nutritional signals to regulate energy balance.Collectively,these findings underscore the important role of RAF1 in AgRP neurons in maintaining energy homeostasis and obesity pathogenesis,positioning it and its downstream pathways as potential therapeutic targets for innovative strategies to combat obesity and related metabolic diseases.展开更多
Bio-based organic room-temperature phosphorescence(RTP)materials have drawn considerable interest due to their potential to replace conventional petroleum-based RTP materials and attain comprehensive full life-cycle c...Bio-based organic room-temperature phosphorescence(RTP)materials have drawn considerable interest due to their potential to replace conventional petroleum-based RTP materials and attain comprehensive full life-cycle carbon reduction,a feat attributable to their renewable,biocompatible,and environmentally friendly characteristics.Bio-based organic RTP materials derived from natural biomass(e.g.,cellulose,lignin,chitosan)or biologically produced substances possess the capacity to spontaneously generate RTP or contribute to its generation.In this paper,the development lineage of bio-based RTP materials is introduced from the above two directions,including different systems,how to construct such systems,and the current progress.With strategies including hydrogen bonding networks,host—vip encapsulation,and polymeric matrices,it achieves RTP lifetimes up to seconds and full visible-band emission.It then explores the application scenarios that emerge from the natural advantages of these materials,including anti-counterfeiting and encryption,environmental monitoring,and bioimaging.Finally,it brieflydiscusses the potential challenges associated with bio-based RTP materials and envisions future development directions for them.While bio-based RTP materials rival petroleum-based counterparts in RTP efficiency,challenges persist:high production costs,poor environmental/thermal stability,and balancing degradability with durability.These sustainable alternatives offer biodegradability,renewability,and reduced lifecycle carbon emissions,utilizing agricultural byproducts(e.g.,corn stalks,shrimp shells)to enhance circular economies.展开更多
Pure organic room temperature phosphorescence(RTP) has been attracting a lot interest recently. So far,many strategies have succeeded in achieving efficient organic RTP materials by increasing the rate of intersystem ...Pure organic room temperature phosphorescence(RTP) has been attracting a lot interest recently. So far,many strategies have succeeded in achieving efficient organic RTP materials by increasing the rate of intersystem crossing(ISC) and suppressing non-radiative transitions. In supramolecular chemistry, the control and regulation of molecular recognition based on the role of the host and vip in supramolecular polymers matrix, has attracted much attention. Recently, researchers have successfully achieved room temperature phosphorescence of pure organic complexes through host-vip interactions. The host molecule specifically includes the phosphorescent vip to reduce non-radiative transitions and enhance room temperature phosphorescence emission. This review aims to describe the developments and achievements of pure organic room temperature phosphorescence systems through the mechanism of host-vip interactions in recent years, and demonstrates the exploration and pursuit of phosphorescent materials of researchers in different fields.展开更多
Background In China's Mainland,patients with neovascular age-related macular degeneration(nAMD)have approximately an 40%prevalence of polypoidal choroidal vasculopathy(PCV).This disease leads to recurrent retinal ...Background In China's Mainland,patients with neovascular age-related macular degeneration(nAMD)have approximately an 40%prevalence of polypoidal choroidal vasculopathy(PCV).This disease leads to recurrent retinal pigment epithelium detachment(PED),extensive subretinal or vitreous hemorrhages,and severe vision loss.China has introduced various treatment modalities in the past years and gained comprehensive experience in treating PCV.Methods A total of 14 retinal specialists nationwide with expertise in PCV were empaneled to prioritize six questions and address their corresponding outcomes,regarding opinions on inactive PCV,choices of anti-vascular endothelial growth factor(anti-VEGF)monotherapy,photodynamic therapy(PDT)monotherapy or combined therapy,patients with persistent subretinal fluid(SRF)or intraretinal fluid(IRF)after loading dose anti-VEGF,and patients with massive subretinal hemorrhage.An evidence synthesis team conducted systematic reviews,which informed the recommendations that address these questions.This guideline used the GRADE(Grading of Recommendations,Assessment,Development,and Evaluation)approach to assess the certainty of evidence and grade the strengths of recommendations.Results The panel proposed the following six conditional recommendations regarding treatment choices.(1)For patients with inactive PCV,we suggest observation over treatment.(2)For treatment-na?ve PCV patients,we suggest either anti-VEGF monotherapy or combined anti-VEGF and PDT rather than PDT monotherapy.(3)For patients with PCV who plan to initiate combined anti-VEGF and PDT treatment,we suggest later/rescue PDT over initiate PDT.(4)For PCV patients who plan to initiate anti-VEGF monotherapy,we suggest the treat and extend(TE)regimen rather than the pro re nata(PRN)regimen following three monthly loading doses.(5)For patients with persistent SRF or IRF on optical coherence tomography(OCT)after three monthly anti-VEGF treatments,we suggest proceeding with anti-VEGF treatment rather than observation.(6)For PCV patients with massive subretinal hemorrhage(equal to or more than four optic disc areas)involving the central macula,we suggest surgery(vitrectomy in combination with tissue-plasminogen activator(tPA)intraocular injection and gas tamponade)rather than anti-VEGF monotherapy.Conclusions Six evidence-based recommendations support optimal care for PCV patients'management.展开更多
Pure organic room-temperature phosphorescence (RTP) materials have been attracting much attention recently. Herein, we report a facile approach combining heavy atom effect and external solvent stimuli to realize RTP. ...Pure organic room-temperature phosphorescence (RTP) materials have been attracting much attention recently. Herein, we report a facile approach combining heavy atom effect and external solvent stimuli to realize RTP. N-Allylquinolinium bromide under 365 nm UV exhibited intense green RTP emission with response upon adding chloroform. This interesting phenomenon endowed N-allylquinolinium bromide great potential as an anti-counterfeiting material.展开更多
· AIM: To investigate the pharmacokinetics and distributions of bevacizumab by intravitreal injection of prepared bevacizumab-poly(L-lactic-co-glycolic acid)(PLGA) microspheres in rabbits, to provide evidence for...· AIM: To investigate the pharmacokinetics and distributions of bevacizumab by intravitreal injection of prepared bevacizumab-poly(L-lactic-co-glycolic acid)(PLGA) microspheres in rabbits, to provide evidence for clinical application of this kind of bevacizumab sustained release dosage form.·METHODS: Bevacizumab was encapsulated into PLGA microsphere via the solid- in- oil- in- hydrophilic oil(S/O/h O) method. Fifteen healthy New Zealand albino-rabbits were used in experiments. The eyes of each rabbit received an intravitreal injection. The left eyes were injected with prepared bevacizumab-PLGA microspheres and the right eyes were injected with bevacizumab solution. After intravitreal injection, rabbits were randomly selected at day 3, 7, 14, 28 and 42 respectively, three animals each day. Then we used immunofluorescence staining to observe the distribution and duration of bevacizumab in rabbit eye tissues, and used the sandwich ELISA to quantify the concentration of free bevacizumab from the rabbit aqueous humor and vitreous after intravitreal injection.·RESULTS: The results show that the concentration of bevacizumab in vitreous and aqueous humor after administration of PLGA formulation was higher than thatof bevacizumab solution. The T1/2of intravitreal injection of bevacizumab-PLGA microspheres is 9.6d in vitreous and 10.2d in aqueous humor, and the T1/2of intravitreal injection of soluble bevacizumab is 3.91 d in vitreous and4.1d in aqueous humor. There were statistical significant difference for comparison the results of the bevacizumab in vitreous and aqueous humor between the left and right eyes(P 【0.05). The AUC0-tof the sustained release dosage form was 1-fold higher than that of the soluble form. The relative bioavailability was raised significantly.The immunofluorescence staining of PLGA-encapsulated bevacizumab(b-PLGA) in rabbit eye tissues was still observed up to 42 d. It was longer than that of the soluble form.· CONCLUSION: The result of this study shows the beneficial effects of PLGA in prolonging the residency of bevacizumab in the vitreous. And the drug delivery system may have potential as a treatment modality for related disease.展开更多
Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination...Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination polymer,to deliver a‘‘smart’’porous material that can response to external stimuli.The obtained dithienylethene-based infinite coordination polymers（named Cu-DTEDBA）share the advantages of both infinite coordination polymers（porosity and stability）and dithienylethene motifs（photochromism）.The physical and chemical properties of Cu-DTEDBA were characterized by FTIR,TEM,SEM,XRD,TGA,UV–vis,EDX and BET.Moreover,the combination of dithienylethene and infinite coordination polymers gives rise to a synergistic effect,which induces functional behaviors of ammonia sensor applications.Both open and closed forms of Cu-DTEDBA exhibit distinct colorimetric change upon exposure to gaseous ammonia,which is not observed in dithienylethene free molecules.展开更多
Room temperature phosphorescence(RTP)generated by small molecules has attracted great attention due to their unique potentials for biosensor,bioimaging and security protection.While,the design of RTP materials is extr...Room temperature phosphorescence(RTP)generated by small molecules has attracted great attention due to their unique potentials for biosensor,bioimaging and security protection.While,the design of RTP materials is extremely challenging for organic small molecules in non-crystalline solid state.Herein,we report a new strategy for achieving non-crystalline organic small molecules with RTP emission by modifying different phosphors onto diphenylalanine or phenylalanine derivatives.Benefiting from the skeletal structure of the amino acid derivatives,there are intermolecular hydrogen bond formation and rigidification effect,thereby minimizing the intermolecular motions and enhancing their RTP performance.展开更多
Organic room-temperature phosphorescence(RTP)materials have garnered considerable attention in the fields of biosensing,optoelectronic devices,and anticounterfeiting because of their substantial Stokes shifts,tunable ...Organic room-temperature phosphorescence(RTP)materials have garnered considerable attention in the fields of biosensing,optoelectronic devices,and anticounterfeiting because of their substantial Stokes shifts,tunable emission wavelengths,and prolonged lifetimes.These materials offer remarkable advantages for biological imaging applications by effectively reducing environmental autofluorescence and enhancing imaging resolution.Recently,host-vip systems have been employed as efficient approaches to fabricate pure-organic RTP materials for bioimaging,providing benefits such as controllable preparation and flexible modulation.Consequently,an increasing number of corresponding studies are being reported;however,a comprehensive systematic review is still lacking.Therefore,we summarize recent advances in the development of pureorganic RTP materials using host-vip systems with regard to bioimaging,including rigid matrices and sensitization.The challenge and potential of RTP for biological imaging are also proposed to promote the biomedical applications of organic RTP materials with excellent optical properties.展开更多
Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations.Localization based on the relative counts of different detectors has b...Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations.Localization based on the relative counts of different detectors has been widely used for all-sky gamma-ray monitors.There are two major methods for this count distribution localization:χ^(2)minimization method and the Bayesian method.Here we propose a modified Bayesian method that could take advantage of both the accuracy of the Bayesian method and the simplicity of the χ^(2)method.With comprehensive simulations,we find that our Bayesian method with Poisson likelihood is generally more applicable for various bursts than the χ^(2)method,especially for weak bursts.We further proposed a location-spectrum iteration approach based on the Bayesian inference,which could alleviate the problems caused by the spectral difference between the burst and location templates.Our method is very suitable for scenarios with limited computation resources or timesensitive applications,such as in-flight localization software,and low-latency localization for rapidly follow-up observations.展开更多
The Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor(GECAM)mission is designed to monitor the Gamma-Ray Bursts(GRBs)associated with gravitational waves and other high-energy transient sources...The Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor(GECAM)mission is designed to monitor the Gamma-Ray Bursts(GRBs)associated with gravitational waves and other high-energy transient sources.The mission consists of two microsatellites which are planned to operate at the opposite sides of the Earth.Each GECAM satellite could detect and localize GRBs in about 8 keV-5 MeV with its 25 Gamma-Ray Detectors(GRDs).In this work,we report the in-flight energy calibration of GRDs using the characteristic gamma-ray lines in the background spectra,and show their performance evolution during the commissioning phase.Besides,a preliminary cross-calibration of energy response with Fermi GBM data is also presented,validating the energy response of GRDs.展开更多
BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic e...BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic evaluation.AIM To determine PBC-associated hub genes and assess their clinical utility for disease prediction.METHODS PBC expression data were obtained from the Gene Expression Omnibus database.Overlapping genes from differential expression analysis and weighted gene coexpression network analysis(WGCNA)were identified as key genes for PBC.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses were performed to explore the potential roles of key genes.Hub genes were identified in protein-protein interaction(PPI)networks using the Degree algorithm in Cytoscape software.The relationship between hub genes and immune cells was investigated.Finally,a Mendelian randomization study was conducted to determine the causal effects of hub genes on PBC.RESULTS We identified 71 overlapping key genes using differential expression analysis and WGCNA.These genes were primarily enriched in pathways related to cytokinecytokine receptor interaction,and Th1,Th2,and Th17 cell differentiation.We utilized Cytoscape software and identified five hub genes(CD247,IL10,CCL5,CCL3,and STAT3)in PPI networks.These hub genes showed a strong correlation with immune cell infiltration in PBC.However,inverse variance weighting analysis did not indicate the causal effects of hub genes on PBC risk.CONCLUSION Hub genes can potentially serve as valuable biomarkers for PBC prediction and treatment,thereby offering significant clinical utility.展开更多
Exploring a new and robust material for proton conduction is of significant importance to the scientific interest and technological importance.Polyoxometalates(POMs)are a class of molecular anion metal oxide clusters ...Exploring a new and robust material for proton conduction is of significant importance to the scientific interest and technological importance.Polyoxometalates(POMs)are a class of molecular anion metal oxide clusters with well-defined structures and diverse properties.Therefore,the design and synthesis of a POM-based material for proton conduction is extremely vital.Herein,a dimeric four tartaric acid-bridged tetra-Zr-incorporated arsenotungstate.展开更多
实船在波浪中的操纵运动数学建模是船舶操纵性预报的难点。在静水中,实船操纵运动建模相对较易实现,在此基础上结合波浪力数学模型,可以产生虚拟的船舶在波浪中的操纵运动数据,再结合有限的波浪环境实测数据,形成虚实融合的数据,从而可...实船在波浪中的操纵运动数学建模是船舶操纵性预报的难点。在静水中,实船操纵运动建模相对较易实现,在此基础上结合波浪力数学模型,可以产生虚拟的船舶在波浪中的操纵运动数据,再结合有限的波浪环境实测数据,形成虚实融合的数据,从而可以利用数据驱动手段,开展实船在波浪中的操纵运动数学建模研究。该文以某船为例,基于静水环境下实船实测数据,使用物理内嵌神经网络(physics informed neural network,PINN)辨识船舶操纵运动MMG方程水动力导数。记及波浪力模型后,形成船舶在波浪中操纵运动数学模型,基于此数学模型构造操纵运动数据,再结合有限的波浪环境数据,利用长短期记忆神经网络(long short term memory,LSTM)开展实船在波浪中的操纵运动在线预报研究。展开更多
In addition to the popularity of laparoscopic gastrectomy(LG),many reconstructive procedures after LG have been reported.Surgical resection and lymphatic dissection determine long-term survival;however,the election of...In addition to the popularity of laparoscopic gastrectomy(LG),many reconstructive procedures after LG have been reported.Surgical resection and lymphatic dissection determine long-term survival;however,the election of a reconstruction procedure determines the postoperative quality of life for patients with gastric cancer(GC).Presently,no consensus exists regarding the optimal reconstructive procedure.In this review,the current state of digestive tract reconstruction after LG is reviewed.According to the determining influence of the tumor site on the procedures of surgical resection and reconstruction,we divide these reconstruction procedures into three categories consistent with the resection procedures.We focus on the technical tips of every reconstruction procedure and examine the surgical outcomes(length of surgery and blood loss)and postoperative complications(anastomotic leakage and stricture)to facilitate gastrointestinal surgeons to understand the merits and demerits of every reconstruction procedure.展开更多
基金support from various sources,including the National Natural Science Foundation of China(Grant Nos.81570774,82070872,92049118,and 82370854)the Junior Thousand Talents Program of China,and the Nanjing Medical University Startup Fund(All awarded to J.L.)support provided by Jiangsu Province's Innovation Personal as well as Innovative and Entrepreneurial Team of Jiangsu Province(Grant No.JSSCTD2021)(All awarded to J.L.).
文摘V-raf-leukemia viral oncogene 1(RAF1),a serine/threonine protein kinase,is well established to play a crucial role in tumorigenesis and cell development.However,the specific role of hypothalamic RAF1 in regulating energy metabolism remains unknown.In this study,we found that the expression of RAF1 was significantly increased in hypothalamic AgRP neurons of diet-induced obesity(DIO)mice.Under normal chow diet feeding,overexpression of Raf1 in AgRP neurons led to obesity in mice characterized by increased body weight,fat mass,and impaired glucose tolerance.Conversely,Raf1 knockout in AgRP neurons protected against diet-induced obesity,reducing fat mass and improving glucose tolerance.Mechanistically,Raf1 activated the MAPK signaling pathway,culminating in the phosphorylation of cAMP response element-binding protein(CREB),which enhanced transcription of Agrp and Npy.Insulin stimulation further potentiated the RAF1-MEK1/2-ERK1/2-CREB axis,highlighting RAF1's role in integrating hormonal and nutritional signals to regulate energy balance.Collectively,these findings underscore the important role of RAF1 in AgRP neurons in maintaining energy homeostasis and obesity pathogenesis,positioning it and its downstream pathways as potential therapeutic targets for innovative strategies to combat obesity and related metabolic diseases.
基金financial support from the National Natural Science Foundation of China(22125803,T2488302,and 22020102006)Postdoctoral Fellowship Program of CPSF(GZB20240220)+2 种基金the Guangxi Department of Science and Technology(AA23062016)Natural Science Foundation of Shanghai(25ZR1402108)Fundamental Research Funds for the Central Universities.
文摘Bio-based organic room-temperature phosphorescence(RTP)materials have drawn considerable interest due to their potential to replace conventional petroleum-based RTP materials and attain comprehensive full life-cycle carbon reduction,a feat attributable to their renewable,biocompatible,and environmentally friendly characteristics.Bio-based organic RTP materials derived from natural biomass(e.g.,cellulose,lignin,chitosan)or biologically produced substances possess the capacity to spontaneously generate RTP or contribute to its generation.In this paper,the development lineage of bio-based RTP materials is introduced from the above two directions,including different systems,how to construct such systems,and the current progress.With strategies including hydrogen bonding networks,host—vip encapsulation,and polymeric matrices,it achieves RTP lifetimes up to seconds and full visible-band emission.It then explores the application scenarios that emerge from the natural advantages of these materials,including anti-counterfeiting and encryption,environmental monitoring,and bioimaging.Finally,it brieflydiscusses the potential challenges associated with bio-based RTP materials and envisions future development directions for them.While bio-based RTP materials rival petroleum-based counterparts in RTP efficiency,challenges persist:high production costs,poor environmental/thermal stability,and balancing degradability with durability.These sustainable alternatives offer biodegradability,renewability,and reduced lifecycle carbon emissions,utilizing agricultural byproducts(e.g.,corn stalks,shrimp shells)to enhance circular economies.
基金financial support from the National Natural Science Foundation of China (NSFC) (Nos. 21788102, 21722603 and 21871083)Project supported by Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03)+2 种基金the Innovation Program of Shanghai Municipal Education Commission (No. 2017-01-07-00-02-E00010)State Key Laboratory for Modification of Chemical Fibers and Polymer Materials (No. KF1803)Donghua University and the Fundamental Research Funds (No. KF1803) for the Central Universities
文摘Pure organic room temperature phosphorescence(RTP) has been attracting a lot interest recently. So far,many strategies have succeeded in achieving efficient organic RTP materials by increasing the rate of intersystem crossing(ISC) and suppressing non-radiative transitions. In supramolecular chemistry, the control and regulation of molecular recognition based on the role of the host and vip in supramolecular polymers matrix, has attracted much attention. Recently, researchers have successfully achieved room temperature phosphorescence of pure organic complexes through host-vip interactions. The host molecule specifically includes the phosphorescent vip to reduce non-radiative transitions and enhance room temperature phosphorescence emission. This review aims to describe the developments and achievements of pure organic room temperature phosphorescence systems through the mechanism of host-vip interactions in recent years, and demonstrates the exploration and pursuit of phosphorescent materials of researchers in different fields.
文摘Background In China's Mainland,patients with neovascular age-related macular degeneration(nAMD)have approximately an 40%prevalence of polypoidal choroidal vasculopathy(PCV).This disease leads to recurrent retinal pigment epithelium detachment(PED),extensive subretinal or vitreous hemorrhages,and severe vision loss.China has introduced various treatment modalities in the past years and gained comprehensive experience in treating PCV.Methods A total of 14 retinal specialists nationwide with expertise in PCV were empaneled to prioritize six questions and address their corresponding outcomes,regarding opinions on inactive PCV,choices of anti-vascular endothelial growth factor(anti-VEGF)monotherapy,photodynamic therapy(PDT)monotherapy or combined therapy,patients with persistent subretinal fluid(SRF)or intraretinal fluid(IRF)after loading dose anti-VEGF,and patients with massive subretinal hemorrhage.An evidence synthesis team conducted systematic reviews,which informed the recommendations that address these questions.This guideline used the GRADE(Grading of Recommendations,Assessment,Development,and Evaluation)approach to assess the certainty of evidence and grade the strengths of recommendations.Results The panel proposed the following six conditional recommendations regarding treatment choices.(1)For patients with inactive PCV,we suggest observation over treatment.(2)For treatment-na?ve PCV patients,we suggest either anti-VEGF monotherapy or combined anti-VEGF and PDT rather than PDT monotherapy.(3)For patients with PCV who plan to initiate combined anti-VEGF and PDT treatment,we suggest later/rescue PDT over initiate PDT.(4)For PCV patients who plan to initiate anti-VEGF monotherapy,we suggest the treat and extend(TE)regimen rather than the pro re nata(PRN)regimen following three monthly loading doses.(5)For patients with persistent SRF or IRF on optical coherence tomography(OCT)after three monthly anti-VEGF treatments,we suggest proceeding with anti-VEGF treatment rather than observation.(6)For PCV patients with massive subretinal hemorrhage(equal to or more than four optic disc areas)involving the central macula,we suggest surgery(vitrectomy in combination with tissue-plasminogen activator(tPA)intraocular injection and gas tamponade)rather than anti-VEGF monotherapy.Conclusions Six evidence-based recommendations support optimal care for PCV patients'management.
基金the financial support from the Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20170009)the National Natural Science Foundation of China(NSFC Nos. 21722603 and 21871083)the Innovation Program of Shanghai Municipal Education Commission
文摘Pure organic room-temperature phosphorescence (RTP) materials have been attracting much attention recently. Herein, we report a facile approach combining heavy atom effect and external solvent stimuli to realize RTP. N-Allylquinolinium bromide under 365 nm UV exhibited intense green RTP emission with response upon adding chloroform. This interesting phenomenon endowed N-allylquinolinium bromide great potential as an anti-counterfeiting material.
基金Supported by National Natural Science Foundation of China (No.30973263, No.81370869)
文摘· AIM: To investigate the pharmacokinetics and distributions of bevacizumab by intravitreal injection of prepared bevacizumab-poly(L-lactic-co-glycolic acid)(PLGA) microspheres in rabbits, to provide evidence for clinical application of this kind of bevacizumab sustained release dosage form.·METHODS: Bevacizumab was encapsulated into PLGA microsphere via the solid- in- oil- in- hydrophilic oil(S/O/h O) method. Fifteen healthy New Zealand albino-rabbits were used in experiments. The eyes of each rabbit received an intravitreal injection. The left eyes were injected with prepared bevacizumab-PLGA microspheres and the right eyes were injected with bevacizumab solution. After intravitreal injection, rabbits were randomly selected at day 3, 7, 14, 28 and 42 respectively, three animals each day. Then we used immunofluorescence staining to observe the distribution and duration of bevacizumab in rabbit eye tissues, and used the sandwich ELISA to quantify the concentration of free bevacizumab from the rabbit aqueous humor and vitreous after intravitreal injection.·RESULTS: The results show that the concentration of bevacizumab in vitreous and aqueous humor after administration of PLGA formulation was higher than thatof bevacizumab solution. The T1/2of intravitreal injection of bevacizumab-PLGA microspheres is 9.6d in vitreous and 10.2d in aqueous humor, and the T1/2of intravitreal injection of soluble bevacizumab is 3.91 d in vitreous and4.1d in aqueous humor. There were statistical significant difference for comparison the results of the bevacizumab in vitreous and aqueous humor between the left and right eyes(P 【0.05). The AUC0-tof the sustained release dosage form was 1-fold higher than that of the soluble form. The relative bioavailability was raised significantly.The immunofluorescence staining of PLGA-encapsulated bevacizumab(b-PLGA) in rabbit eye tissues was still observed up to 42 d. It was longer than that of the soluble form.· CONCLUSION: The result of this study shows the beneficial effects of PLGA in prolonging the residency of bevacizumab in the vitreous. And the drug delivery system may have potential as a treatment modality for related disease.
基金supported by National Basic Research Program of China (No. 2013CB733501)National Natural Science Foundation of China (Nos. 91334203, 21376074, 21402050)Fundamental Research Funds for the Central Universities of China (No. WK1314008)
文摘Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination polymer,to deliver a‘‘smart’’porous material that can response to external stimuli.The obtained dithienylethene-based infinite coordination polymers（named Cu-DTEDBA）share the advantages of both infinite coordination polymers（porosity and stability）and dithienylethene motifs（photochromism）.The physical and chemical properties of Cu-DTEDBA were characterized by FTIR,TEM,SEM,XRD,TGA,UV–vis,EDX and BET.Moreover,the combination of dithienylethene and infinite coordination polymers gives rise to a synergistic effect,which induces functional behaviors of ammonia sensor applications.Both open and closed forms of Cu-DTEDBA exhibit distinct colorimetric change upon exposure to gaseous ammonia,which is not observed in dithienylethene free molecules.
基金the National Natural Science Foundation of China(NSFC,Nos.21788102,21722603 and 21871083)Shanghai Municipal Science and Technology Major Project(No.2018SHZDZX03)+2 种基金‘Shu Guang’Project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation(No.19SG26)the Innovation Program of Shanghai Municipal Education Commission(No.2017-01-07-00-02-E00010)Fundamental Research Funds for the Central Universities。
文摘Room temperature phosphorescence(RTP)generated by small molecules has attracted great attention due to their unique potentials for biosensor,bioimaging and security protection.While,the design of RTP materials is extremely challenging for organic small molecules in non-crystalline solid state.Herein,we report a new strategy for achieving non-crystalline organic small molecules with RTP emission by modifying different phosphors onto diphenylalanine or phenylalanine derivatives.Benefiting from the skeletal structure of the amino acid derivatives,there are intermolecular hydrogen bond formation and rigidification effect,thereby minimizing the intermolecular motions and enhancing their RTP performance.
基金the financial support from the National Natural Science Foundation of China (Nos. 22125803, 22020102006 and 22307036)a project supported by the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03)+2 种基金the Program of Shanghai Academic/Technology Research Leader (No. 20XD1421300)China Postdoctoral Science Foundation (No. 2023M731079)the Fundamental Research Funds for the Central Universities
文摘Organic room-temperature phosphorescence(RTP)materials have garnered considerable attention in the fields of biosensing,optoelectronic devices,and anticounterfeiting because of their substantial Stokes shifts,tunable emission wavelengths,and prolonged lifetimes.These materials offer remarkable advantages for biological imaging applications by effectively reducing environmental autofluorescence and enhancing imaging resolution.Recently,host-vip systems have been employed as efficient approaches to fabricate pure-organic RTP materials for bioimaging,providing benefits such as controllable preparation and flexible modulation.Consequently,an increasing number of corresponding studies are being reported;however,a comprehensive systematic review is still lacking.Therefore,we summarize recent advances in the development of pureorganic RTP materials using host-vip systems with regard to bioimaging,including rigid matrices and sensitization.The challenge and potential of RTP for biological imaging are also proposed to promote the biomedical applications of organic RTP materials with excellent optical properties.
基金supported by the National Key R&D Program of China(2021YFA0718500)support from the Strategic Priority Research Program on Space Science,the Chinese Academy of Sciences(grant Nos.XDA15360102,XDA15360300,XDA15052700 and E02212A02S)+1 种基金the National Natural Science Foundation of China(grant Nos.12173038 and U2038106)the National HEP Data Center(grant No.E029S2S1)。
文摘Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations.Localization based on the relative counts of different detectors has been widely used for all-sky gamma-ray monitors.There are two major methods for this count distribution localization:χ^(2)minimization method and the Bayesian method.Here we propose a modified Bayesian method that could take advantage of both the accuracy of the Bayesian method and the simplicity of the χ^(2)method.With comprehensive simulations,we find that our Bayesian method with Poisson likelihood is generally more applicable for various bursts than the χ^(2)method,especially for weak bursts.We further proposed a location-spectrum iteration approach based on the Bayesian inference,which could alleviate the problems caused by the spectral difference between the burst and location templates.Our method is very suitable for scenarios with limited computation resources or timesensitive applications,such as in-flight localization software,and low-latency localization for rapidly follow-up observations.
基金supported by the Strategic Priority Program on Space Science,the Chinese Academy of Sciences,grant Nos.XDA15360102,XDA15360300 and E02212A02Sthe National Natural Science Foundation of China(Project:12061131007)。
文摘The Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor(GECAM)mission is designed to monitor the Gamma-Ray Bursts(GRBs)associated with gravitational waves and other high-energy transient sources.The mission consists of two microsatellites which are planned to operate at the opposite sides of the Earth.Each GECAM satellite could detect and localize GRBs in about 8 keV-5 MeV with its 25 Gamma-Ray Detectors(GRDs).In this work,we report the in-flight energy calibration of GRDs using the characteristic gamma-ray lines in the background spectra,and show their performance evolution during the commissioning phase.Besides,a preliminary cross-calibration of energy response with Fermi GBM data is also presented,validating the energy response of GRDs.
基金Supported by School-Level Key Projects at Bengbu Medical College,No.2021byzd109。
文摘BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic evaluation.AIM To determine PBC-associated hub genes and assess their clinical utility for disease prediction.METHODS PBC expression data were obtained from the Gene Expression Omnibus database.Overlapping genes from differential expression analysis and weighted gene coexpression network analysis(WGCNA)were identified as key genes for PBC.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses were performed to explore the potential roles of key genes.Hub genes were identified in protein-protein interaction(PPI)networks using the Degree algorithm in Cytoscape software.The relationship between hub genes and immune cells was investigated.Finally,a Mendelian randomization study was conducted to determine the causal effects of hub genes on PBC.RESULTS We identified 71 overlapping key genes using differential expression analysis and WGCNA.These genes were primarily enriched in pathways related to cytokinecytokine receptor interaction,and Th1,Th2,and Th17 cell differentiation.We utilized Cytoscape software and identified five hub genes(CD247,IL10,CCL5,CCL3,and STAT3)in PPI networks.These hub genes showed a strong correlation with immune cell infiltration in PBC.However,inverse variance weighting analysis did not indicate the causal effects of hub genes on PBC risk.CONCLUSION Hub genes can potentially serve as valuable biomarkers for PBC prediction and treatment,thereby offering significant clinical utility.
基金supported by the National Natural Science Foundation of China(Nos.22071043 and 22203027)Shanxi Province Science Foundation(No.202303021211194)the program for the(Reserved)Discipline Leaders of Taiyuan Institute of Technology.
文摘Exploring a new and robust material for proton conduction is of significant importance to the scientific interest and technological importance.Polyoxometalates(POMs)are a class of molecular anion metal oxide clusters with well-defined structures and diverse properties.Therefore,the design and synthesis of a POM-based material for proton conduction is extremely vital.Herein,a dimeric four tartaric acid-bridged tetra-Zr-incorporated arsenotungstate.
文摘实船在波浪中的操纵运动数学建模是船舶操纵性预报的难点。在静水中,实船操纵运动建模相对较易实现,在此基础上结合波浪力数学模型,可以产生虚拟的船舶在波浪中的操纵运动数据,再结合有限的波浪环境实测数据,形成虚实融合的数据,从而可以利用数据驱动手段,开展实船在波浪中的操纵运动数学建模研究。该文以某船为例,基于静水环境下实船实测数据,使用物理内嵌神经网络(physics informed neural network,PINN)辨识船舶操纵运动MMG方程水动力导数。记及波浪力模型后,形成船舶在波浪中操纵运动数学模型,基于此数学模型构造操纵运动数据,再结合有限的波浪环境数据,利用长短期记忆神经网络(long short term memory,LSTM)开展实船在波浪中的操纵运动在线预报研究。
文摘In addition to the popularity of laparoscopic gastrectomy(LG),many reconstructive procedures after LG have been reported.Surgical resection and lymphatic dissection determine long-term survival;however,the election of a reconstruction procedure determines the postoperative quality of life for patients with gastric cancer(GC).Presently,no consensus exists regarding the optimal reconstructive procedure.In this review,the current state of digestive tract reconstruction after LG is reviewed.According to the determining influence of the tumor site on the procedures of surgical resection and reconstruction,we divide these reconstruction procedures into three categories consistent with the resection procedures.We focus on the technical tips of every reconstruction procedure and examine the surgical outcomes(length of surgery and blood loss)and postoperative complications(anastomotic leakage and stricture)to facilitate gastrointestinal surgeons to understand the merits and demerits of every reconstruction procedure.
