In order to improve the mechanical properties without reducing its flame retardancy of phenolic foams(PFs),9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide(DOPO)was introduced in the structure of cashew nut shell li...In order to improve the mechanical properties without reducing its flame retardancy of phenolic foams(PFs),9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide(DOPO)was introduced in the structure of cashew nut shell liquid(CNSL)to improve its flame retardant,and the product of DOPO grafting CNSL(DOPO-g-CNSL)was obtained to modify phenolic resin,and to prepare DOPO-g-CNSL modified PFs(DCMPFs).The structures of DOPO-g-CNSL were verified by Fourier transform infrared spectroscopy(FT-IR)and nuclear magnetic resonance(1H-NMR).Compared with CNSL,thermal stability of DOPO-g-CNSL was decreased and Ti decreased by 3.53%,but the residual carbon(800°C)was increased by 35.05%.Compared with pure PF,the mechanical properties,toughness and flame retardancy of DCMPFs were increased when the ratio of DOPO-g-CNSL to phenol(DOPO-g-CNSL/P)was no more than 10%.With the dosage of DOPO-g-CNSL/P increased,Ti of DCMPFs was slightly increased,but the carbon residues(800°C)were almost unchanged.And the cell sizes of DCMPFs were basically the same as the pure PF.By comprehensive analysis,the suitable dosage of DOPO-g-CNSL/P was no more than 10%.展开更多
目的 探讨“医物技”多层次教学模式在肿瘤放射治疗学临床实习教学中的应用效果。方法 选取空军军医大学2018级五年制临床医学专业88名学员为研究对象,随机分为试验组(n=44)和对照组(n=44);以外科学中的肿瘤章节(立体定向放射外科)为临...目的 探讨“医物技”多层次教学模式在肿瘤放射治疗学临床实习教学中的应用效果。方法 选取空军军医大学2018级五年制临床医学专业88名学员为研究对象,随机分为试验组(n=44)和对照组(n=44);以外科学中的肿瘤章节(立体定向放射外科)为临床实习教学内容;试验组采用“医物技”多层次教学方式,对照组采用传统教学方式;采用实习结束后和结束3个月时的2次考试成绩和问卷调查进行结果评价。结果 试验组两次考核的成绩均好于对照组(实习结束时:76.91±4.62 vs. 67.80±7.76,P<0.001;实习结束后3个月:70.68±4.47 vs. 62.00±4.47,P<0.001)。问卷调查结果显示,试验组学员在学习兴趣、知识理解、能力提升和视野拓展等方面的评分均高于对照组(依次为7.50±0.88 vs. 5.66±1.14、7.61±0.72 vs. 5.77±1.16、7.07±0.10 vs. 5.43±0.76、6.02±0.95 vs. 4.52±0.88,均P<0.001)。结论 “医物技”多层次教学模式应用于肿瘤放射治疗学临床实习教学能提高学员学习的积极性、知识掌握的全面性和准确性,以及长期记忆的牢固性。展开更多
This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenz...This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.展开更多
Paraphrase generation strives to generate high-quality and diverse expressions of a given text,a domain where diffusion models excel.Though SOTA diffusion generation reconciles generation quality and diversity,textual...Paraphrase generation strives to generate high-quality and diverse expressions of a given text,a domain where diffusion models excel.Though SOTA diffusion generation reconciles generation quality and diversity,textual diffusion suffers from a truncation issue that hinders efficiency and quality control.In this work,we propose Latent Diffusion Paraphraser(LDP),a novel paraphrase generation by modeling a controllable diffusion process given a learned latent space.LDP achieves superior generation efficiency compared to its diffusion counterparts.It can facilitate only input segments to ensure paraphrase semantics,improving the results without external features.Experiments show that LDP better reconciles paraphrase generation quality and diversity than baselines.Further analysis shows that our method is also helpful to other similar text generations and domain adaptations.展开更多
Movement disorders of the human foot-ankle complex are a common occurrence,owing to the altered joint mechanics during foot-ground interactions.Diagnostics of such movement disorders will require quantitative tools to...Movement disorders of the human foot-ankle complex are a common occurrence,owing to the altered joint mechanics during foot-ground interactions.Diagnostics of such movement disorders will require quantitative tools to evaluate in-vivo foot motions,in particular to the multi-segment/joint foot kinematics(MSFK),during gait.Unfortunately,current MSFK analysis largely rely on conventional technologies,such as skin-marker based motion capturing,video fluoroscopy and dynamic 3D scanning,being extremely time-consuming and costly.In this work,a novel movement tracking method,named the point-cloud foot analysis(PFA),was implemented with multi-view depth sensors,to allow fast evaluations of 3D motions of the foot-ankle complex during gait.Quantitative analysis obtained by the PFA methods and their accuracy relative to the conventional MSFK analysis methods were evaluated.The 3D surface reconstructions of the foot-ankle complex were achieved with a RMSE less than 2 mm.It was proven to be feasible to track multi-segment foot motions in both healthy and diseased subjects during walking conditions,with the processing time decreased from more than 4-6 h to less than 6 min for the entire flow of the contact phase analysis.The PFA method can be useful for fast evaluations of the movement disorders of the foot-ankle complex in diagnostics and design of therapeutic interventions and rehabilitation programs for clinical applications.展开更多
基金by The Youth Innovation Fund of Nanjing Forestry University(CX2016011)Nanjing Forestry University High-Level(High-Educated)Talents Scientific Research Funds(GXL2014033)。
文摘In order to improve the mechanical properties without reducing its flame retardancy of phenolic foams(PFs),9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide(DOPO)was introduced in the structure of cashew nut shell liquid(CNSL)to improve its flame retardant,and the product of DOPO grafting CNSL(DOPO-g-CNSL)was obtained to modify phenolic resin,and to prepare DOPO-g-CNSL modified PFs(DCMPFs).The structures of DOPO-g-CNSL were verified by Fourier transform infrared spectroscopy(FT-IR)and nuclear magnetic resonance(1H-NMR).Compared with CNSL,thermal stability of DOPO-g-CNSL was decreased and Ti decreased by 3.53%,but the residual carbon(800°C)was increased by 35.05%.Compared with pure PF,the mechanical properties,toughness and flame retardancy of DCMPFs were increased when the ratio of DOPO-g-CNSL to phenol(DOPO-g-CNSL/P)was no more than 10%.With the dosage of DOPO-g-CNSL/P increased,Ti of DCMPFs was slightly increased,but the carbon residues(800°C)were almost unchanged.And the cell sizes of DCMPFs were basically the same as the pure PF.By comprehensive analysis,the suitable dosage of DOPO-g-CNSL/P was no more than 10%.
文摘目的 探讨“医物技”多层次教学模式在肿瘤放射治疗学临床实习教学中的应用效果。方法 选取空军军医大学2018级五年制临床医学专业88名学员为研究对象,随机分为试验组(n=44)和对照组(n=44);以外科学中的肿瘤章节(立体定向放射外科)为临床实习教学内容;试验组采用“医物技”多层次教学方式,对照组采用传统教学方式;采用实习结束后和结束3个月时的2次考试成绩和问卷调查进行结果评价。结果 试验组两次考核的成绩均好于对照组(实习结束时:76.91±4.62 vs. 67.80±7.76,P<0.001;实习结束后3个月:70.68±4.47 vs. 62.00±4.47,P<0.001)。问卷调查结果显示,试验组学员在学习兴趣、知识理解、能力提升和视野拓展等方面的评分均高于对照组(依次为7.50±0.88 vs. 5.66±1.14、7.61±0.72 vs. 5.77±1.16、7.07±0.10 vs. 5.43±0.76、6.02±0.95 vs. 4.52±0.88,均P<0.001)。结论 “医物技”多层次教学模式应用于肿瘤放射治疗学临床实习教学能提高学员学习的积极性、知识掌握的全面性和准确性,以及长期记忆的牢固性。
文摘This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.
基金supported by the National Natural Science Foundation of China(Grant Nos.62376116,62176120)the Liaoning Provincial Research Foundation for Basic Research(No.2022-KF-26-02)a research project of Nanjing University-China Mobile Joint Institute.
文摘Paraphrase generation strives to generate high-quality and diverse expressions of a given text,a domain where diffusion models excel.Though SOTA diffusion generation reconciles generation quality and diversity,textual diffusion suffers from a truncation issue that hinders efficiency and quality control.In this work,we propose Latent Diffusion Paraphraser(LDP),a novel paraphrase generation by modeling a controllable diffusion process given a learned latent space.LDP achieves superior generation efficiency compared to its diffusion counterparts.It can facilitate only input segments to ensure paraphrase semantics,improving the results without external features.Experiments show that LDP better reconciles paraphrase generation quality and diversity than baselines.Further analysis shows that our method is also helpful to other similar text generations and domain adaptations.
基金supported by National Key Research and Development Project,China(No.2022YFC2009500)Shanghai Science and Technology Development Funds(No.20S31901000&No.21511102200)Medical Engineering Fund of Fudan University(No.yg2021-019).
文摘Movement disorders of the human foot-ankle complex are a common occurrence,owing to the altered joint mechanics during foot-ground interactions.Diagnostics of such movement disorders will require quantitative tools to evaluate in-vivo foot motions,in particular to the multi-segment/joint foot kinematics(MSFK),during gait.Unfortunately,current MSFK analysis largely rely on conventional technologies,such as skin-marker based motion capturing,video fluoroscopy and dynamic 3D scanning,being extremely time-consuming and costly.In this work,a novel movement tracking method,named the point-cloud foot analysis(PFA),was implemented with multi-view depth sensors,to allow fast evaluations of 3D motions of the foot-ankle complex during gait.Quantitative analysis obtained by the PFA methods and their accuracy relative to the conventional MSFK analysis methods were evaluated.The 3D surface reconstructions of the foot-ankle complex were achieved with a RMSE less than 2 mm.It was proven to be feasible to track multi-segment foot motions in both healthy and diseased subjects during walking conditions,with the processing time decreased from more than 4-6 h to less than 6 min for the entire flow of the contact phase analysis.The PFA method can be useful for fast evaluations of the movement disorders of the foot-ankle complex in diagnostics and design of therapeutic interventions and rehabilitation programs for clinical applications.
