Objective:To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis(KOA)model.Methods: Rabbits(n=18)were randomly divided into control,KOA,and KOA+acup...Objective:To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis(KOA)model.Methods: Rabbits(n=18)were randomly divided into control,KOA,and KOA+acupotomy(Apo)groups(n=6).The rabbits in the KOA and Apo groups were modeled using the modified Videman's method for 6 weeks.After modeling,the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis,vastus lateralis,rectus femoris,biceps femoris,and anserine bursa tendons around the knee.The behavior of all animals was recorded,rectus femoris tissue was obtained,and histomorphological changes were observed using Masson staining and transmission electron microscopy.The expression of transforming growth factor-β1(TGF-β1),Smad 3,Smad 7,fibrillar collagen types I(Col-I)and III(Col-III)was detected using Western blot and real-time polymerase chain reaction(RT-PCR).Results: Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris,compared with the KOA group(P=.034).Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA,thus preventing skeletal muscle fibrosis.Western blot and RT-PCR analysis revealed that in the Apo group,Col-I,and Col-III protein levels were significantly lower than those in the KOA group(both P<.01),same as Col-I and Col-III mRNA levels(P=.0031;P=.0046).Compared with the KOA group,the protein levels of TGF-β1 and Smad 3 were significantly reduced(both P<.01),as were the mRNA levels of TGF-β1 and Smad 3(P=.0007;P=.0011).Conversely,the levels of protein and mRNA of Smad 7 were significantly higher than that in the KOA group(P<.01;P=.0271).Conclusion: Acupotomy could alleviate skeletal muscle fibrosis and delay KOA progress by inhibiting collagen deposition through the TGF-β/Smad pathway in the skeletal muscle of KOA rabbits.展开更多
The development of a combined photocatalytic system with peroxymonosulfate (PMS) has great potential applications in the degradation and treatment of aqueous organic pollutants. Herein, a Co_(3)O_(4)-ZnO/rGO was prepa...The development of a combined photocatalytic system with peroxymonosulfate (PMS) has great potential applications in the degradation and treatment of aqueous organic pollutants. Herein, a Co_(3)O_(4)-ZnO/rGO was prepared by a hydrothermal method using cobalt acetate, zinc acetate, and reduced graphene oxide (rGO) as the main raw materials. The physical and chemical characteristics of the obtained catalyst were analyzed using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS),scanning electron microscopy (SEM), and Fourier transform infrared (FT-IR). The photocatalytic features and capacities of the catalytic materials to activate PMS were investigated. Co_(3)O_(4)-ZnO/rGO exhibited stronger photocatalytic activity and ability to activate PMS than Co_(3)O_(4)/rGO or ZnO/rGO, and significantly improved the ability of PMS and photocatalysis to synergistically degrade rhodamine B (RhB), with a degradation rate of 90.40% within 40 min. The mechanism of RhB degradation was proposed based on characterization of materials, evaluation of RhB degradation efficiency, and analysis of the active species involved. The unique particle/sheet structure of Co_(3)O_(4)-ZnO/rGO provides a large number of active sites, and the formation of heterojunctions between Co_(3)O_(4) and ZnO improves carrier separation and transport in the reaction system. Our study offers a reference for designing more effective heterojunction catalysts based on the combination of PMS and photocatalytic technology.展开更多
AIM:To evaluate impact of radiation therapy dose escalation through intensity modulated radiation therapy with simultaneous integrated boost(IMRT-SIB).METHODS:We retrospectively reviewed the patients who underwent fou...AIM:To evaluate impact of radiation therapy dose escalation through intensity modulated radiation therapy with simultaneous integrated boost(IMRT-SIB).METHODS:We retrospectively reviewed the patients who underwent four-dimensional-based IMRT-SIBbased neoadjuvant chemoradiation protocol.During the concurrent chemoradiation therapy,radiation therapy was through IMRT-SIB delivered in 28 consecutive daily fractions with total radiation doses of 56 Gy to tumor and 5040 Gy dose-painted to clinical tumor volume,with a regimen at the discretion of the treating medical oncologist.This was followed by surgical tumor resection.We analyzed pathological completion response(p CR)rates its relationship with overall survival and event-freesurvival.RESULTS:Seventeen patients underwent dose escalation with the IMRT-SIB protocol between 2007 and 2014 and their records were available for analysis.Among the IMRT-SIB-treated patients,the toxicity appeared mild,the most common side effects were grade 1-3 esophagitis(46%)and pneumonitis(11.7%).There were no cardiac events.The Ro resection rate was 94%(n=16),the p CR rate was 47%(n=8),and the postoperative morbidity was zero.There was one mediastinal failure found,one patient had local failure at the anastomosis site,and the majority of failures were distant in the lung or bone.The 3-year diseasefree survival and overall survival rates were 41%(n=7)and 53%(n=9),respectively.CONCLUSION:The dose escalation through IMRT-SIB in the chemoradiation regimen seems responsible for down-staging the distal esophageal with well-tolerated complications.展开更多
1 IntroductionOver the last several decades, although there have beenadvances in the treatment of diseases such as children leukemia, Hodgkin's disease, and testicular cancer, however,
Objective:To investigate whether acupotomy could inhibit subchondral bone remodeling in knee osteoarthritis(KOA)rabbits by regulating the activity of osteoblasts and osteoclasts.Methods:KOA rabbits were prepared by im...Objective:To investigate whether acupotomy could inhibit subchondral bone remodeling in knee osteoarthritis(KOA)rabbits by regulating the activity of osteoblasts and osteoclasts.Methods:KOA rabbits were prepared by immobilization for 6 and 9 weeks by Videman method.Nine groups of rabbits(control,6 weeks and 9 weeks model,6 weeks and 9 weeks acupotomy,6 weeks and 9 weeks electroacupuncture,and 6 weeks and 9 weeks drug groups)received acupotomy,electroacupuncture and risedronate sodium intervention,respectively,for 3 weeks.Results:Acupotomy can inhibit the activity of osteoclasts and osteoblasts in subchondral bone by reducing the proteins expression of cathepsin K(CK)and tartrate-resistant acid phosphatase(TRAP)and decreasing the proteins expression of osteocalcin(OCN)and alkaline phosphatase(ALP),to intercept the abnormal bone resorption and bone formation of subchondral bone in 6-week and 9-week immobilization-induced KOA rabbits.Conclusion:These findings indicated that acupotomy may be more advantageous than risedronate sodium intervention in modulating subchondral bone remodeling in KOA rabbits,especially in 9-week immobilization-induced KOA rabbits.展开更多
Background There is a large group of deaf-mutes worldwide, and sign language is a major communication tool in this community. It is necessary for deaf-mutes to be able to communicate with others who are capable of hea...Background There is a large group of deaf-mutes worldwide, and sign language is a major communication tool in this community. It is necessary for deaf-mutes to be able to communicate with others who are capable of hearing, and hearing people also need to understand sign language, which produces a great demand for sign language tuition. Even though there have already been a large number of books written about sign language, it is inefficient to learn sign language through reading alone, and the same can be said on watching videos. To solve this problem, we developed a smartphone-based interactive Chinese sign language teaching system that facilitates sign language learning. Methods The system provides a learner with some learning modes and captures the learner's actions using the front camera of the smartphone. At present, the system provides a vocabulary set with 1000 frequently used words, and the learner can evaluate his/her sign action by subjective or objective comparison. In the mode of word recognition, the users can play any word within the vocabulary, and the system will return the top three retrieved candidates;thus, it can remind the learners what the sign is. Results This system provides interactive learning to enable a user to efficiently learn sign language. The system adopts an algorithm based on point cloud recognition to evaluate a user's sign and costs about 700ms of inference time for each sample, which meets the real-time requirements. Conclusion This interactive learning system decreases the communication barriers between deaf-mutes and hearing people.展开更多
Hands play an important role in our daily life.We use our hands for manipulation in working,emphasis in speaking,communication in non-verbal environment,etc.Hand gesture not only uses for simple commands in traffic co...Hands play an important role in our daily life.We use our hands for manipulation in working,emphasis in speaking,communication in non-verbal environment,etc.Hand gesture not only uses for simple commands in traffic control,but also extends as a kind of language-sign language.In the areas of VR/AR and HCI,understanding hand and its action can greatly improve user experience.This covers a broad topics related to hands,including hand detection,tracking,hand pose estimation,gesture recognition,and sign language translation.Four papers are collected in this issue.They cover different topics related to hand and gesture.展开更多
1 Introduction.Learning visual speech representations from talking face videos is an important problem for several speech-related tasks,such as lip reading,talking face generation,and audiovisual speech separation[1,2...1 Introduction.Learning visual speech representations from talking face videos is an important problem for several speech-related tasks,such as lip reading,talking face generation,and audiovisual speech separation[1,2].The key difficulty lies in tackling speech-irrelevant factors presented in the videos,such as lighting,resolution,viewpoints,and head motion.展开更多
Robust face representation is imperative to highly accurate face recognition. In this work, we propose an open source face recognition method with deep representation named as VIPLFaceNet, which is a lO-layer deep con...Robust face representation is imperative to highly accurate face recognition. In this work, we propose an open source face recognition method with deep representation named as VIPLFaceNet, which is a lO-layer deep convolu- tional neural network with seven convolutional layers and three fully-connected layers. Compared with the well-known AlexNet, our VIPLFaceNet takes only 20% training time and 60% testing time, but achieves 40% drop in error rate on the real-world face recognition benchmark LFW. Our VIPLFaceNet achieves 98.60% mean accuracy on LFW us- ing one single network. An open-source C＋＋ SDK based on VIPLFaceNet is released under BSD license. The SDK takes about 150ms to process one face image in a single thread on an i7 desktop CPU. VIPLFaceNet provides a state-of-the-art start point for both academic and industrial face recognition applications.展开更多
In traditional hybrid DC circuit breakers(HCBs)schemes,complex equipment is usually required to ensure current commutation from mechanical to semiconductor switches,which cause not only additional construction costs a...In traditional hybrid DC circuit breakers(HCBs)schemes,complex equipment is usually required to ensure current commutation from mechanical to semiconductor switches,which cause not only additional construction costs and volume but also additional losses and maintenance work.Different from this condition,a low-loss and compact HCB scheme that does not use separate current commutation equipment is proposed in this study.By rationally using the charge and discharge of the snubber capacitor,the self-commutated semiconductor switch(SCS)module can integrate both the current commutation and shutdown functions,thereby greatly reducing cost and volume.The working process is discussed and analyzed in detail,and then a 10-kA prototype is developed and tested,which verifies the feasibility and effectiveness of the proposed scheme.Index Terms-HVDC circuit breakers,hybrid DC circuit breakers(HCBs),self-commutated semiconductor switch(SCS).展开更多
Although the conventional active appearance model (AAM) has achieved some success for face alignment, it still suffers from the generalization problem when be applied to unseen subjects and images. To deal with the ge...Although the conventional active appearance model (AAM) has achieved some success for face alignment, it still suffers from the generalization problem when be applied to unseen subjects and images. To deal with the gem eralization problem of AAM, we first reformulate the original AAM as sparsity-regularized AAM, which can achieve more compact/better shape and appearance priors by selecting nearest neighbors as the bases of the shape and appearance model. To speed up the fitting procedure, the sparsity in sparsity?regularized AAM is approximated by using the locality (i.e., AT-nearest neighbor), and thus inducing the locality-constrained active appearance model (LC-AAM). The LC-AAM solves a constrained AAM-like fitting problem with the K-nearest neighbors as the bases of shape and appearance model. To alleviate the adverse influence of inaccurate AT-nearest neighbor results, the locality constraint is further embedded in the discriminative fitting method denoted as LC-DFM, which can find better K-nearest neighbor results by employing shape-indexed feature, and can also tolerate some inaccurate neighbors benefited from the regression model rather than the generative model in AAM. Extensive experiments on several datasets demonstrate that our methods outperform the state-of-the-arts in both detection accuracy and generalization ability.展开更多
To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2(Adanti-ERK2)gene therapy upon chronic allograft nephropathy(CAN)of rats,male Lewis(LEW,RT11)rats received male Fisher(F344,RT11v...To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2(Adanti-ERK2)gene therapy upon chronic allograft nephropathy(CAN)of rats,male Lewis(LEW,RT11)rats received male Fisher(F344,RT11v1)renal allografts.The recipients were divided into three groups:(1)empty control group;(2)vector control group;(3)gene therapy group.All recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation.Morphometric analysis was used to determine thefibrosis of grafts.Immunohistochemistry was used to detect the expression of E-Cadherin,Vimentin,TβR I and the infiltration of CD4+T lymphocyte,CD8+T lymphocyte and ED-1+monocytes.Enzyme linked immunosorbent assay(ELISA)was used to detect TGF-β1 in serum.The grafts in the empty control group and vector control group showed CAN.There was less E-Cadherin in renal tubular epithelial cells in the empty control group but more Vimentin and TβR I.In the gene therapy group,thefibrosis was ameliorated and fewer T lymphocytes and ED-1+monocytes infiltrated in the interstitium.There was no significant difference in the expression of E-Cadherin between the gene therapy group and normal rats.Compared with the empty control group,the expression of TGF-β1 in the gene therapy group was down-regulated.Adanti-ERK2 gene therapy protects the renal allograft and attenuates graftfibrosis,which may be correlated with a decreased renal tubular epithelial mesenchymal transition,a decreased infiltration of CD4+T lymphocyte,CD8+T lymphocytes and ED-1+monocytes in renal interstitium,and the down-regulated TGF-β1 expression.展开更多
基金supported by the National Natural Science Foundation of China(82074523)the National Natural Youth Science Foundation of China(82004448).
文摘Objective:To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis(KOA)model.Methods: Rabbits(n=18)were randomly divided into control,KOA,and KOA+acupotomy(Apo)groups(n=6).The rabbits in the KOA and Apo groups were modeled using the modified Videman's method for 6 weeks.After modeling,the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis,vastus lateralis,rectus femoris,biceps femoris,and anserine bursa tendons around the knee.The behavior of all animals was recorded,rectus femoris tissue was obtained,and histomorphological changes were observed using Masson staining and transmission electron microscopy.The expression of transforming growth factor-β1(TGF-β1),Smad 3,Smad 7,fibrillar collagen types I(Col-I)and III(Col-III)was detected using Western blot and real-time polymerase chain reaction(RT-PCR).Results: Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris,compared with the KOA group(P=.034).Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA,thus preventing skeletal muscle fibrosis.Western blot and RT-PCR analysis revealed that in the Apo group,Col-I,and Col-III protein levels were significantly lower than those in the KOA group(both P<.01),same as Col-I and Col-III mRNA levels(P=.0031;P=.0046).Compared with the KOA group,the protein levels of TGF-β1 and Smad 3 were significantly reduced(both P<.01),as were the mRNA levels of TGF-β1 and Smad 3(P=.0007;P=.0011).Conversely,the levels of protein and mRNA of Smad 7 were significantly higher than that in the KOA group(P<.01;P=.0271).Conclusion: Acupotomy could alleviate skeletal muscle fibrosis and delay KOA progress by inhibiting collagen deposition through the TGF-β/Smad pathway in the skeletal muscle of KOA rabbits.
基金the National Natural Science Foundation of China(No.21906012).
文摘The development of a combined photocatalytic system with peroxymonosulfate (PMS) has great potential applications in the degradation and treatment of aqueous organic pollutants. Herein, a Co_(3)O_(4)-ZnO/rGO was prepared by a hydrothermal method using cobalt acetate, zinc acetate, and reduced graphene oxide (rGO) as the main raw materials. The physical and chemical characteristics of the obtained catalyst were analyzed using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS),scanning electron microscopy (SEM), and Fourier transform infrared (FT-IR). The photocatalytic features and capacities of the catalytic materials to activate PMS were investigated. Co_(3)O_(4)-ZnO/rGO exhibited stronger photocatalytic activity and ability to activate PMS than Co_(3)O_(4)/rGO or ZnO/rGO, and significantly improved the ability of PMS and photocatalysis to synergistically degrade rhodamine B (RhB), with a degradation rate of 90.40% within 40 min. The mechanism of RhB degradation was proposed based on characterization of materials, evaluation of RhB degradation efficiency, and analysis of the active species involved. The unique particle/sheet structure of Co_(3)O_(4)-ZnO/rGO provides a large number of active sites, and the formation of heterojunctions between Co_(3)O_(4) and ZnO improves carrier separation and transport in the reaction system. Our study offers a reference for designing more effective heterojunction catalysts based on the combination of PMS and photocatalytic technology.
文摘AIM:To evaluate impact of radiation therapy dose escalation through intensity modulated radiation therapy with simultaneous integrated boost(IMRT-SIB).METHODS:We retrospectively reviewed the patients who underwent four-dimensional-based IMRT-SIBbased neoadjuvant chemoradiation protocol.During the concurrent chemoradiation therapy,radiation therapy was through IMRT-SIB delivered in 28 consecutive daily fractions with total radiation doses of 56 Gy to tumor and 5040 Gy dose-painted to clinical tumor volume,with a regimen at the discretion of the treating medical oncologist.This was followed by surgical tumor resection.We analyzed pathological completion response(p CR)rates its relationship with overall survival and event-freesurvival.RESULTS:Seventeen patients underwent dose escalation with the IMRT-SIB protocol between 2007 and 2014 and their records were available for analysis.Among the IMRT-SIB-treated patients,the toxicity appeared mild,the most common side effects were grade 1-3 esophagitis(46%)and pneumonitis(11.7%).There were no cardiac events.The Ro resection rate was 94%(n=16),the p CR rate was 47%(n=8),and the postoperative morbidity was zero.There was one mediastinal failure found,one patient had local failure at the anastomosis site,and the majority of failures were distant in the lung or bone.The 3-year diseasefree survival and overall survival rates were 41%(n=7)and 53%(n=9),respectively.CONCLUSION:The dose escalation through IMRT-SIB in the chemoradiation regimen seems responsible for down-staging the distal esophageal with well-tolerated complications.
文摘1 IntroductionOver the last several decades, although there have beenadvances in the treatment of diseases such as children leukemia, Hodgkin's disease, and testicular cancer, however,
基金supported by the Beijing Municipal Natural Science Foundation(7192110)。
文摘Objective:To investigate whether acupotomy could inhibit subchondral bone remodeling in knee osteoarthritis(KOA)rabbits by regulating the activity of osteoblasts and osteoclasts.Methods:KOA rabbits were prepared by immobilization for 6 and 9 weeks by Videman method.Nine groups of rabbits(control,6 weeks and 9 weeks model,6 weeks and 9 weeks acupotomy,6 weeks and 9 weeks electroacupuncture,and 6 weeks and 9 weeks drug groups)received acupotomy,electroacupuncture and risedronate sodium intervention,respectively,for 3 weeks.Results:Acupotomy can inhibit the activity of osteoclasts and osteoblasts in subchondral bone by reducing the proteins expression of cathepsin K(CK)and tartrate-resistant acid phosphatase(TRAP)and decreasing the proteins expression of osteocalcin(OCN)and alkaline phosphatase(ALP),to intercept the abnormal bone resorption and bone formation of subchondral bone in 6-week and 9-week immobilization-induced KOA rabbits.Conclusion:These findings indicated that acupotomy may be more advantageous than risedronate sodium intervention in modulating subchondral bone remodeling in KOA rabbits,especially in 9-week immobilization-induced KOA rabbits.
文摘Background There is a large group of deaf-mutes worldwide, and sign language is a major communication tool in this community. It is necessary for deaf-mutes to be able to communicate with others who are capable of hearing, and hearing people also need to understand sign language, which produces a great demand for sign language tuition. Even though there have already been a large number of books written about sign language, it is inefficient to learn sign language through reading alone, and the same can be said on watching videos. To solve this problem, we developed a smartphone-based interactive Chinese sign language teaching system that facilitates sign language learning. Methods The system provides a learner with some learning modes and captures the learner's actions using the front camera of the smartphone. At present, the system provides a vocabulary set with 1000 frequently used words, and the learner can evaluate his/her sign action by subjective or objective comparison. In the mode of word recognition, the users can play any word within the vocabulary, and the system will return the top three retrieved candidates;thus, it can remind the learners what the sign is. Results This system provides interactive learning to enable a user to efficiently learn sign language. The system adopts an algorithm based on point cloud recognition to evaluate a user's sign and costs about 700ms of inference time for each sample, which meets the real-time requirements. Conclusion This interactive learning system decreases the communication barriers between deaf-mutes and hearing people.
文摘Hands play an important role in our daily life.We use our hands for manipulation in working,emphasis in speaking,communication in non-verbal environment,etc.Hand gesture not only uses for simple commands in traffic control,but also extends as a kind of language-sign language.In the areas of VR/AR and HCI,understanding hand and its action can greatly improve user experience.This covers a broad topics related to hands,including hand detection,tracking,hand pose estimation,gesture recognition,and sign language translation.Four papers are collected in this issue.They cover different topics related to hand and gesture.
基金the National Natural Science Foundation of China(Grant Nos.62276247,62076250)。
文摘1 Introduction.Learning visual speech representations from talking face videos is an important problem for several speech-related tasks,such as lip reading,talking face generation,and audiovisual speech separation[1,2].The key difficulty lies in tackling speech-irrelevant factors presented in the videos,such as lighting,resolution,viewpoints,and head motion.
基金This work was partially supported by the National Basic Research Program of China （973 Program） （2015CB351802）, and the National Natural Science Foundation of China （Grant Nos. 61402443, 61390511, 61379083, 61222211）.
文摘Robust face representation is imperative to highly accurate face recognition. In this work, we propose an open source face recognition method with deep representation named as VIPLFaceNet, which is a lO-layer deep convolu- tional neural network with seven convolutional layers and three fully-connected layers. Compared with the well-known AlexNet, our VIPLFaceNet takes only 20% training time and 60% testing time, but achieves 40% drop in error rate on the real-world face recognition benchmark LFW. Our VIPLFaceNet achieves 98.60% mean accuracy on LFW us- ing one single network. An open-source C＋＋ SDK based on VIPLFaceNet is released under BSD license. The SDK takes about 150ms to process one face image in a single thread on an i7 desktop CPU. VIPLFaceNet provides a state-of-the-art start point for both academic and industrial face recognition applications.
基金supported by the National Natural Science Foundation of China(No.52107004)the China Postdoctoral Science Foundation(No.2020M680484 and No.2021T140201)the Fundamental Research Funds for the Central Universities(No.2021MS003)。
文摘In traditional hybrid DC circuit breakers(HCBs)schemes,complex equipment is usually required to ensure current commutation from mechanical to semiconductor switches,which cause not only additional construction costs and volume but also additional losses and maintenance work.Different from this condition,a low-loss and compact HCB scheme that does not use separate current commutation equipment is proposed in this study.By rationally using the charge and discharge of the snubber capacitor,the self-commutated semiconductor switch(SCS)module can integrate both the current commutation and shutdown functions,thereby greatly reducing cost and volume.The working process is discussed and analyzed in detail,and then a 10-kA prototype is developed and tested,which verifies the feasibility and effectiveness of the proposed scheme.Index Terms-HVDC circuit breakers,hybrid DC circuit breakers(HCBs),self-commutated semiconductor switch(SCS).
基金the National Natural Science Foundation of China (Grant Nos. 61650202, 61402443, 61672496)the Strategic Priority Research Program of the CAS (XDB02070004).
文摘Although the conventional active appearance model (AAM) has achieved some success for face alignment, it still suffers from the generalization problem when be applied to unseen subjects and images. To deal with the gem eralization problem of AAM, we first reformulate the original AAM as sparsity-regularized AAM, which can achieve more compact/better shape and appearance priors by selecting nearest neighbors as the bases of the shape and appearance model. To speed up the fitting procedure, the sparsity in sparsity?regularized AAM is approximated by using the locality (i.e., AT-nearest neighbor), and thus inducing the locality-constrained active appearance model (LC-AAM). The LC-AAM solves a constrained AAM-like fitting problem with the K-nearest neighbors as the bases of shape and appearance model. To alleviate the adverse influence of inaccurate AT-nearest neighbor results, the locality constraint is further embedded in the discriminative fitting method denoted as LC-DFM, which can find better K-nearest neighbor results by employing shape-indexed feature, and can also tolerate some inaccurate neighbors benefited from the regression model rather than the generative model in AAM. Extensive experiments on several datasets demonstrate that our methods outperform the state-of-the-arts in both detection accuracy and generalization ability.
基金partially supported by the National Basic Research Program of China (2015CB351802)the National Natural Science Foundation of China (Grant Nos.61390511,61222211, 61379083,61271445)+1 种基金the Strategic Priority Research Program of the CAS (XDB02070004) the Youth Innovation Promotion Association CAS (2015085).
基金supported by the National Natural Science Foundation of China(Grant No.30300324).
文摘To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2(Adanti-ERK2)gene therapy upon chronic allograft nephropathy(CAN)of rats,male Lewis(LEW,RT11)rats received male Fisher(F344,RT11v1)renal allografts.The recipients were divided into three groups:(1)empty control group;(2)vector control group;(3)gene therapy group.All recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation.Morphometric analysis was used to determine thefibrosis of grafts.Immunohistochemistry was used to detect the expression of E-Cadherin,Vimentin,TβR I and the infiltration of CD4+T lymphocyte,CD8+T lymphocyte and ED-1+monocytes.Enzyme linked immunosorbent assay(ELISA)was used to detect TGF-β1 in serum.The grafts in the empty control group and vector control group showed CAN.There was less E-Cadherin in renal tubular epithelial cells in the empty control group but more Vimentin and TβR I.In the gene therapy group,thefibrosis was ameliorated and fewer T lymphocytes and ED-1+monocytes infiltrated in the interstitium.There was no significant difference in the expression of E-Cadherin between the gene therapy group and normal rats.Compared with the empty control group,the expression of TGF-β1 in the gene therapy group was down-regulated.Adanti-ERK2 gene therapy protects the renal allograft and attenuates graftfibrosis,which may be correlated with a decreased renal tubular epithelial mesenchymal transition,a decreased infiltration of CD4+T lymphocyte,CD8+T lymphocytes and ED-1+monocytes in renal interstitium,and the down-regulated TGF-β1 expression.
