Vanadium alloys, especially that of V4Cr4Ti, are supposed as the promising candidate structural material for fusion applications for their good thermal properties, high temperature strength and the inherent low activa...Vanadium alloys, especially that of V4Cr4Ti, are supposed as the promising candidate structural material for fusion applications for their good thermal properties, high temperature strength and the inherent low activation characteristics. However, in展开更多
With the deepening of enterprise reform, the vast majority of internal management systems have developed towards standardization, and many enterprises have vigorously promoted the innovation of management mode accordi...With the deepening of enterprise reform, the vast majority of internal management systems have developed towards standardization, and many enterprises have vigorously promoted the innovation of management mode according to the actual development needs, so that the overall level of enterprise management has been continuously improved. Budget management is an important part of enterprise's financial accounting work. By vigorously strengthening and improving enterprise's budget management, it has a strong basic and supporting role in promoting enterprise's financial accounting work and enterprise's strategic development. Especially, many enterprises use budget management to promote enterprise's high-quality development, optimize resource allocation and prevent operational risks. Through "excellent mechanism, excellent management and control, and excellent system", we will constantly improve the budget management level, enhance the market competitiveness of enterprises, and realize their healthy, stable and long-term development. However, we should also see that under the new historical conditions, especially with the increasing stimulation of market competition, some enterprises still have many problems in budget management, especially the lack of strategic and comprehensive budget management, which directly leads to the inability of budget management to create favorable conditions for the sustainable development of enterprises. Based on the brief analysis of the importance of enterprise budget management, this paper focuses on the achievements and outstanding problems of enterprise budget management, and finally puts forward optimization countermeasures on how to strengthen and improve enterprise budget management, aiming at providing reference for promoting enterprise budget management to achieve better results.展开更多
In the era of big data, enterprise fiscal and taxation management has ushered in a new direction. Through big data technology, it can realize the further optimization of fiscal and taxation management, the full integr...In the era of big data, enterprise fiscal and taxation management has ushered in a new direction. Through big data technology, it can realize the further optimization of fiscal and taxation management, the full integration of online and offline and the full penetration of the front and back offices, thus promoting the effective construction of internet pluss tax management mode. Under this development background, enterprises should actively respond to the call of fiscal and taxation policy reform, apply big data technology in fiscal and taxation management, dig deep into high-quality commercial value information, continuously improve the informatization level of fiscal and taxation treatment, effectively control fiscal and taxation risk factors, promote financial information sharing and then meet the development needs of enterprises, solve various problems in fiscal and taxation management, effectively control tax costs and improve business efficiency.展开更多
Tungsten is envisaged as one of the main candidate materials for divertor plate of ITER and future fusion reactors. Due to D-T reaction, PFMs would suffer helium irradiation from plasma additional to the high heat loa...Tungsten is envisaged as one of the main candidate materials for divertor plate of ITER and future fusion reactors. Due to D-T reaction, PFMs would suffer helium irradiation from plasma additional to the high heat loads. Helium retention and thermal desorption behavior are largely concerned.展开更多
目的微小RNA相关单核苷酸多态性(microRNA-related single nucleotide polymorphism,miR-SNP)与肿瘤的发生发展关系密切。本研究对中国人群中miR-SNP与结直肠癌(colorectal cancer,CRC)发生发展及化疗疗效的关系进行研究。方法首先,选...目的微小RNA相关单核苷酸多态性(microRNA-related single nucleotide polymorphism,miR-SNP)与肿瘤的发生发展关系密切。本研究对中国人群中miR-SNP与结直肠癌(colorectal cancer,CRC)发生发展及化疗疗效的关系进行研究。方法首先,选择较小等位基因频率>10%的miR-SNP;采用单碱基延伸结合基质辅助激光解吸离子化-飞行时间-质谱法(MALDI-TOF-MS)检测2013-05-10-2015-10-22无锡市第四人民医院收治的504例CRC患者和714名健康对照的miR-SNPs基因型。统计分析miR-SNP基因型与CRC临床病理参数及化疗疗效的关联性。结果rs61992671基因型在病例组和健康对照组中的分布差异有统计学意义,χ2=6.44,P=0.026;A/G型个体患CRC的风险为3.2%,比A/A型(5.5%)更低,OR=1.77。经性别和年龄调整后,rs12402181A等位基因携带者的肿瘤体积比G/G型更大,χ2=3.85,OR=1.67,P=0.047,矫正OR=1.68,矫正P=0.045;rs12402181杂合子患结肠癌的风险为54.4%,比纯合子(45.0%)高,χ2=3.95,P=0.047,矫正P=0.018。rs67106263基因型与CRC的肿瘤分化程度显著相关,χ2=8.46,P=0.020,矫正P=0.026;与G/G型相比,G/A型(OR=2.35,矫正OR=2.30)、A/A型(OR=2.78,矫正OR=2.69)和A等位基因携带者(OR=2.38,矫正OR=2.33)的肿瘤分化程度均明显更差。此外,在接受基于5-氟尿嘧啶化疗的CRC患者中,rs61992671基因型与化疗效果有关联,χ2=2.75,P=0.041,矫正P=0.046。结论 rs61992671与CRC的发生和5-氟尿嘧啶化疗疗效有关联;rs12402181和rs67106263与CRC的发展有关联。展开更多
Vitellogenin (Vtg) is the precursor of yolk protein. Its expression and secretion are estrogen-regulated and are crucial for oocyte maturation. An in vitro xenoestro- gen screening model was established by measuring V...Vitellogenin (Vtg) is the precursor of yolk protein. Its expression and secretion are estrogen-regulated and are crucial for oocyte maturation. An in vitro xenoestro- gen screening model was established by measuring Vtg in- duction in cultured primary hepatocytes from crucian carp. Vtg production was detected by biotin-avidin sandwich ELISA method while Vtg and cytochrome P4501A1 (CYP1A1) mRNA induction were measured by semi- quan- titative PCR-primer dropping technique. Vtg and Vtg mRNA were dose-dependently induced by diethylstilbestrol (DES, 0.2—200 ng/mL) in hepatocytes of crucian carp. Co-treatment of the DES-induced hepatocytes with either 2,3,7,8-TCDD (TCDD, 0.1—4 pg/mL) or benzo[a]pyrene (B[a]P, 5—1000 ng/mL) resulted in a reduction of Vtg pro- duction and an increment of CYP1A1 mRNA expression both in a dose dependent manner, indicating the anti-estro- genic effects of the compounds. However, at lower tested concentrations, TCDD (0.1, 0.2 pg/mL), B[a]P (5 ng/mL) seemed to have a potentiating effect on Vtg expression and secretion, although by their own these compounds had no observable estrogenic effect on Vtg induction. Tamoxifen (a selective estrogen receptor modulators, 1 nmol/L—1 μmol/L), and β-naphtho-flavone (β-NF, an aryl hydrocarbon receptor inducing compounds, 2.5—1000 ng/mL) also were employed to study the possible interactions in DES-induced Vtg ex- pression. In co-treatment of the DES-induced hepatocytes with β-NF or tamoxifen, the decrease in Vtg production did parallel induction of CYP1A1 for β-NF, but tamoxifen inhib- ited Vtg induction did not parallel induced CYP1A1 expres- sion in all test concentrations. On the contrary, it was found that in co-treatment of the TCDD-induced hepatocytes with DES, TCDD induced CYP1A1 mRNA production was inhib- ited by DES also. These results implicated a possible cross talk between estrogen receptor- and aryl hydrocarbon re- ceptor-mediated pathways in the hepatocytes.展开更多
RNA interference (RNAi) causes degradation of targeted endogenous RNA in many diverse organisms. To investigate the effect of dsRNA on silkworm cells, we trans- fected three kinds of synthetic dsRNAs of 435 bp(Ap1), 3...RNA interference (RNAi) causes degradation of targeted endogenous RNA in many diverse organisms. To investigate the effect of dsRNA on silkworm cells, we trans- fected three kinds of synthetic dsRNAs of 435 bp(Ap1), 300 bp(Ap2) and 399 bp(AH) in length against the various regions of BmNPV’s DNA polymerase gene and DNA helicase gene, which are indispensable for viral replication in silkworm cells by TransMessengerTM transfection Reagent. Results indicated that in the experiment where silkworm cells were infected with wild-strain BmNPV of the three dsRNAs, Ap2 and AH can effectively suppress the replication of virus, but Ap1 had no effect on the inhibition of viral replication. Ap2 and AH can reduce the infective titer of BmNPV with a peak change of approximately 3—4 logs on day 4 post-infection. The results of reverse transcript polymerase chain reaction (RT-PCR) and DNA dot blotting also indicated that the ex- pression level of the two target genes and the quantity of viral DNA both distinctly decreased under the influence of Ap2 or AH. Furthermore, using fluorescence microscopy we analyzed the distribution patterns of dsRNA. Our studies revealed that a majority of dsRNA was localized in the nu- clear periphery discontinuously after 24 h of transfection.展开更多
In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer em- bryos, the enucleated MⅡ oocytes, enucleated zygotes and...In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer em- bryos, the enucleated MⅡ oocytes, enucleated zygotes and 2-cell blastomere were used to produce cloned mouse em-bryos. Using fibroblast deriving from C57/BL6 ear tissue as nuclear donor, we produced cloned embryos by transferring the fibroblast nuclei into enucleated KM mouse oocytes (sin-gle nuclear transfer, SNT), transferring pronuclei from the SNT embryos into enucleated KM zygotes (nuclear into zy-gote, NZ), and 2-cell blastomere nuclei from SNT embryos into enucleated KM mouse oocytes (nuclear into oocytes, NO); tetraploid embryos (tetraploid embryos, TE) were ob-tained by fusing two blastomeres, one is from the SNT cloned embryos, and the other from normal 2-cell KM mouse em-bryos. In group SNT, the cloned embryos could not develop beyond 8-cell stage and the rate of 8-cell stage is only 0.3%; in group NO, the reconstructed embryos could develop to morula stage, the rate of 8-cell stage was significantly greater than that of SNT group (P < 0.05); in group NZ, the devel-opment rate was further improved, and the reconstructed embryos could develop into blastocyst stage, the rate of blastocyst was 1.9%; in group TE, as high as 62.3% of the reconstructed embryos could develop into blastocyst. Results suggested that different nuclear recipients could significantly affect the developmental potential of cloned mouse embryos; KM MⅡ oocyte cytoplasm was not so effective as zygotes to reprogram the mouse somatic cell nuclei; serial nuclear transfer could improve the developmental potential of cloned mouse embryos.展开更多
文摘Vanadium alloys, especially that of V4Cr4Ti, are supposed as the promising candidate structural material for fusion applications for their good thermal properties, high temperature strength and the inherent low activation characteristics. However, in
文摘With the deepening of enterprise reform, the vast majority of internal management systems have developed towards standardization, and many enterprises have vigorously promoted the innovation of management mode according to the actual development needs, so that the overall level of enterprise management has been continuously improved. Budget management is an important part of enterprise's financial accounting work. By vigorously strengthening and improving enterprise's budget management, it has a strong basic and supporting role in promoting enterprise's financial accounting work and enterprise's strategic development. Especially, many enterprises use budget management to promote enterprise's high-quality development, optimize resource allocation and prevent operational risks. Through "excellent mechanism, excellent management and control, and excellent system", we will constantly improve the budget management level, enhance the market competitiveness of enterprises, and realize their healthy, stable and long-term development. However, we should also see that under the new historical conditions, especially with the increasing stimulation of market competition, some enterprises still have many problems in budget management, especially the lack of strategic and comprehensive budget management, which directly leads to the inability of budget management to create favorable conditions for the sustainable development of enterprises. Based on the brief analysis of the importance of enterprise budget management, this paper focuses on the achievements and outstanding problems of enterprise budget management, and finally puts forward optimization countermeasures on how to strengthen and improve enterprise budget management, aiming at providing reference for promoting enterprise budget management to achieve better results.
文摘In the era of big data, enterprise fiscal and taxation management has ushered in a new direction. Through big data technology, it can realize the further optimization of fiscal and taxation management, the full integration of online and offline and the full penetration of the front and back offices, thus promoting the effective construction of internet pluss tax management mode. Under this development background, enterprises should actively respond to the call of fiscal and taxation policy reform, apply big data technology in fiscal and taxation management, dig deep into high-quality commercial value information, continuously improve the informatization level of fiscal and taxation treatment, effectively control fiscal and taxation risk factors, promote financial information sharing and then meet the development needs of enterprises, solve various problems in fiscal and taxation management, effectively control tax costs and improve business efficiency.
文摘Tungsten is envisaged as one of the main candidate materials for divertor plate of ITER and future fusion reactors. Due to D-T reaction, PFMs would suffer helium irradiation from plasma additional to the high heat loads. Helium retention and thermal desorption behavior are largely concerned.
文摘Vitellogenin (Vtg) is the precursor of yolk protein. Its expression and secretion are estrogen-regulated and are crucial for oocyte maturation. An in vitro xenoestro- gen screening model was established by measuring Vtg in- duction in cultured primary hepatocytes from crucian carp. Vtg production was detected by biotin-avidin sandwich ELISA method while Vtg and cytochrome P4501A1 (CYP1A1) mRNA induction were measured by semi- quan- titative PCR-primer dropping technique. Vtg and Vtg mRNA were dose-dependently induced by diethylstilbestrol (DES, 0.2—200 ng/mL) in hepatocytes of crucian carp. Co-treatment of the DES-induced hepatocytes with either 2,3,7,8-TCDD (TCDD, 0.1—4 pg/mL) or benzo[a]pyrene (B[a]P, 5—1000 ng/mL) resulted in a reduction of Vtg pro- duction and an increment of CYP1A1 mRNA expression both in a dose dependent manner, indicating the anti-estro- genic effects of the compounds. However, at lower tested concentrations, TCDD (0.1, 0.2 pg/mL), B[a]P (5 ng/mL) seemed to have a potentiating effect on Vtg expression and secretion, although by their own these compounds had no observable estrogenic effect on Vtg induction. Tamoxifen (a selective estrogen receptor modulators, 1 nmol/L—1 μmol/L), and β-naphtho-flavone (β-NF, an aryl hydrocarbon receptor inducing compounds, 2.5—1000 ng/mL) also were employed to study the possible interactions in DES-induced Vtg ex- pression. In co-treatment of the DES-induced hepatocytes with β-NF or tamoxifen, the decrease in Vtg production did parallel induction of CYP1A1 for β-NF, but tamoxifen inhib- ited Vtg induction did not parallel induced CYP1A1 expres- sion in all test concentrations. On the contrary, it was found that in co-treatment of the TCDD-induced hepatocytes with DES, TCDD induced CYP1A1 mRNA production was inhib- ited by DES also. These results implicated a possible cross talk between estrogen receptor- and aryl hydrocarbon re- ceptor-mediated pathways in the hepatocytes.
文摘RNA interference (RNAi) causes degradation of targeted endogenous RNA in many diverse organisms. To investigate the effect of dsRNA on silkworm cells, we trans- fected three kinds of synthetic dsRNAs of 435 bp(Ap1), 300 bp(Ap2) and 399 bp(AH) in length against the various regions of BmNPV’s DNA polymerase gene and DNA helicase gene, which are indispensable for viral replication in silkworm cells by TransMessengerTM transfection Reagent. Results indicated that in the experiment where silkworm cells were infected with wild-strain BmNPV of the three dsRNAs, Ap2 and AH can effectively suppress the replication of virus, but Ap1 had no effect on the inhibition of viral replication. Ap2 and AH can reduce the infective titer of BmNPV with a peak change of approximately 3—4 logs on day 4 post-infection. The results of reverse transcript polymerase chain reaction (RT-PCR) and DNA dot blotting also indicated that the ex- pression level of the two target genes and the quantity of viral DNA both distinctly decreased under the influence of Ap2 or AH. Furthermore, using fluorescence microscopy we analyzed the distribution patterns of dsRNA. Our studies revealed that a majority of dsRNA was localized in the nu- clear periphery discontinuously after 24 h of transfection.
基金the Climbing Project of the Ministry of Science and Technology of China (Grant No. 97021109-2) and the Major Project of Knowledge Innovation of the Chinese Academy of Sciences (Grant No. KSCX1-05-01)
文摘In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer em- bryos, the enucleated MⅡ oocytes, enucleated zygotes and 2-cell blastomere were used to produce cloned mouse em-bryos. Using fibroblast deriving from C57/BL6 ear tissue as nuclear donor, we produced cloned embryos by transferring the fibroblast nuclei into enucleated KM mouse oocytes (sin-gle nuclear transfer, SNT), transferring pronuclei from the SNT embryos into enucleated KM zygotes (nuclear into zy-gote, NZ), and 2-cell blastomere nuclei from SNT embryos into enucleated KM mouse oocytes (nuclear into oocytes, NO); tetraploid embryos (tetraploid embryos, TE) were ob-tained by fusing two blastomeres, one is from the SNT cloned embryos, and the other from normal 2-cell KM mouse em-bryos. In group SNT, the cloned embryos could not develop beyond 8-cell stage and the rate of 8-cell stage is only 0.3%; in group NO, the reconstructed embryos could develop to morula stage, the rate of 8-cell stage was significantly greater than that of SNT group (P < 0.05); in group NZ, the devel-opment rate was further improved, and the reconstructed embryos could develop into blastocyst stage, the rate of blastocyst was 1.9%; in group TE, as high as 62.3% of the reconstructed embryos could develop into blastocyst. Results suggested that different nuclear recipients could significantly affect the developmental potential of cloned mouse embryos; KM MⅡ oocyte cytoplasm was not so effective as zygotes to reprogram the mouse somatic cell nuclei; serial nuclear transfer could improve the developmental potential of cloned mouse embryos.
