本试验旨在研究枯草芽孢杆菌(BS)、蒙脱石(MMT)及其互作对产蛋鸡生产性能、养分表观利用率和肠黏膜养分转运载体基因表达的影响。采用2×2双因子随机试验设计,主效应分别为BS(0、0.5 g/kg)、MMT(0、0.5 g/kg)及二者互作。选择360只2...本试验旨在研究枯草芽孢杆菌(BS)、蒙脱石(MMT)及其互作对产蛋鸡生产性能、养分表观利用率和肠黏膜养分转运载体基因表达的影响。采用2×2双因子随机试验设计,主效应分别为BS(0、0.5 g/kg)、MMT(0、0.5 g/kg)及二者互作。选择360只29周龄健康的罗曼粉壳蛋鸡,随机分为4组(每组6个重复,每个重复15只),分别饲喂基础饲粮(对照组)、基础饲粮+0.5 g/kg BS(BS组)、基础饲粮+0.5 g/kg M M T(M M T组)、基础饲粮+0.5 g/kg M M T+0.5 g/kg BS(M M T+BS组)。预试期7 d,正试期70 d。结果显示:1)与对照组相比,各添加组产蛋鸡产蛋率均显著提高(P<0.05);MMT组和MMT+BS组日产蛋量显著提高(P<0.05);MMT+BS组料蛋比显著降低(P<0.05)。2)饲粮中添加BS显著提高了干物质、粗脂肪、能量和粗蛋白质的表观利用率(P<0.05);BS组和MMT+BS组能量表观利用率显著高于对照组(P<0.05)。3)与对照组相比,各添加组产蛋鸡空肠黏膜碱性氨基酸转运载体1 m RNA表达量均显著提高(P<0.05);BS和M M T互作对产蛋鸡空肠黏膜易化葡萄糖转运载体2和碱性氨基酸转运载体1m RNA表达量有显著影响(P<0.05)。由此可见,饲粮中单独添加BS和M M T及二者联用均能够提高产蛋鸡的产蛋率和日产蛋量;饲粮中添加BS能够提高产蛋鸡对饲粮中干物质、粗脂肪、粗蛋白质和能量的表观利用率;饲粮中单独添加BS和MMT及二者联用均能够上调产蛋鸡肠黏膜碱性氨基酸转运载体1基因的表达。展开更多
Hippocampal neuronal apoptosis accompanied by impairment of cognitive function occurs in primary diabetic encephalopathy.In this study,we investigated the neuroprotective mechanism of the iridoid glycoside,aucubin,usi...Hippocampal neuronal apoptosis accompanied by impairment of cognitive function occurs in primary diabetic encephalopathy.In this study,we investigated the neuroprotective mechanism of the iridoid glycoside,aucubin,using rats(n=8).Diabetes mellitus was induced in the rats by intraperitoneal(i.p.)injection of streptozotocin(60 mg/kg body weight).After 65 d,half of the DM rats were administered aucubin(5 mg/kg;i.p.)for 15 d,yielding treatment DM+A.A third group of rats received no strepto-zotocin or aucibin,and served as controls(CON).Encephalopathy was assessed using Y-maze be-havioral testing.Rats were euthanized on Day 87,and hippocampi were excised for visual(light and transmission electron microscopic)and immunochemical(Western blot;immunohistochemical)as-sessments of the CA1 subfield for apoptosis and expression of regulatory proteins Bcl-2 and Bax.Treatment responses to all the parameters examined(body weight,plasma glucose,Y-maze error rates,pyramidal cell ultrastructure,proportions of apoptotic cells,levels of expression of Bcl-2 and Bax,and survivability of neuronal cells)were identical:there were highly significant differences between DM and CON groups(P<0.001),but the effects were significantly moderated(P<0.01)in DM+A compared with DM.These findings confirm the association of apoptosis with the encephalopathic effects of diabetes mellitus,and suggest a major role of the expression levels of Bcl-2 and Bax in the regulation of apop-totic cell death.All of the results suggest that aucubin could effectively inhibit apoptosis by modulating the expressions of Bcl-2 and Bax genes.展开更多
文摘本试验旨在研究枯草芽孢杆菌(BS)、蒙脱石(MMT)及其互作对产蛋鸡生产性能、养分表观利用率和肠黏膜养分转运载体基因表达的影响。采用2×2双因子随机试验设计,主效应分别为BS(0、0.5 g/kg)、MMT(0、0.5 g/kg)及二者互作。选择360只29周龄健康的罗曼粉壳蛋鸡,随机分为4组(每组6个重复,每个重复15只),分别饲喂基础饲粮(对照组)、基础饲粮+0.5 g/kg BS(BS组)、基础饲粮+0.5 g/kg M M T(M M T组)、基础饲粮+0.5 g/kg M M T+0.5 g/kg BS(M M T+BS组)。预试期7 d,正试期70 d。结果显示:1)与对照组相比,各添加组产蛋鸡产蛋率均显著提高(P<0.05);MMT组和MMT+BS组日产蛋量显著提高(P<0.05);MMT+BS组料蛋比显著降低(P<0.05)。2)饲粮中添加BS显著提高了干物质、粗脂肪、能量和粗蛋白质的表观利用率(P<0.05);BS组和MMT+BS组能量表观利用率显著高于对照组(P<0.05)。3)与对照组相比,各添加组产蛋鸡空肠黏膜碱性氨基酸转运载体1 m RNA表达量均显著提高(P<0.05);BS和M M T互作对产蛋鸡空肠黏膜易化葡萄糖转运载体2和碱性氨基酸转运载体1m RNA表达量有显著影响(P<0.05)。由此可见,饲粮中单独添加BS和M M T及二者联用均能够提高产蛋鸡的产蛋率和日产蛋量;饲粮中添加BS能够提高产蛋鸡对饲粮中干物质、粗脂肪、粗蛋白质和能量的表观利用率;饲粮中单独添加BS和MMT及二者联用均能够上调产蛋鸡肠黏膜碱性氨基酸转运载体1基因的表达。
基金the National Natural Science Foundation of China(Grant No.30371053)the National Outstanding Youth Foundation of China(Grant No.30125034)the Scientific and Technological Plan Project of Dalian City(Grant No.2003B3NS024)
文摘Hippocampal neuronal apoptosis accompanied by impairment of cognitive function occurs in primary diabetic encephalopathy.In this study,we investigated the neuroprotective mechanism of the iridoid glycoside,aucubin,using rats(n=8).Diabetes mellitus was induced in the rats by intraperitoneal(i.p.)injection of streptozotocin(60 mg/kg body weight).After 65 d,half of the DM rats were administered aucubin(5 mg/kg;i.p.)for 15 d,yielding treatment DM+A.A third group of rats received no strepto-zotocin or aucibin,and served as controls(CON).Encephalopathy was assessed using Y-maze be-havioral testing.Rats were euthanized on Day 87,and hippocampi were excised for visual(light and transmission electron microscopic)and immunochemical(Western blot;immunohistochemical)as-sessments of the CA1 subfield for apoptosis and expression of regulatory proteins Bcl-2 and Bax.Treatment responses to all the parameters examined(body weight,plasma glucose,Y-maze error rates,pyramidal cell ultrastructure,proportions of apoptotic cells,levels of expression of Bcl-2 and Bax,and survivability of neuronal cells)were identical:there were highly significant differences between DM and CON groups(P<0.001),but the effects were significantly moderated(P<0.01)in DM+A compared with DM.These findings confirm the association of apoptosis with the encephalopathic effects of diabetes mellitus,and suggest a major role of the expression levels of Bcl-2 and Bax in the regulation of apop-totic cell death.All of the results suggest that aucubin could effectively inhibit apoptosis by modulating the expressions of Bcl-2 and Bax genes.
